scholarly journals Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 194 ◽  
Author(s):  
Ayesha Manzoor ◽  
Touqeer Ahmad ◽  
Muhammad Bashir ◽  
Ishfaq Hafiz ◽  
Cristian Silvestri
Keyword(s):  
Chromosome Doubling ◽  
Ornamental Plants ◽  
Plant Size ◽  
Unreduced Gametes ◽  
Chromosome Counting ◽  
Morphological And Physiological Traits ◽  
Routine Procedures ◽  
Mitotic Inhibitor ◽  
Intense Color

Polyploidy has the utmost importance in horticulture for the development of new ornamental varieties with desirable morphological traits referring to plant size and vigor, leaf thickness, larger flowers with thicker petals, intense color of leaves and flowers, long lasting flowers, compactness, dwarfness and restored fertility. Polyploidy may occur naturally due to the formation of unreduced gametes or can be artificially induced by doubling the number of chromosomes in somatic cells. Usually, natural polyploid plants are unavailable, so polyploidy is induced synthetically with the help of mitotic inhibitors. Colchicine is a widely used mitotic inhibitor for the induction of polyploidy in plants during their cell division by inhibiting the chromosome segregation. Different plant organs like seeds, apical meristems, flower buds, and roots can be used to induce polyploidy through many application methods such as dipping/soaking, dropping or cotton wool. Flow cytometry and chromosome counting, with an observation of morphological and physiological traits are routine procedures for the determination of ploidy level in plants.

scholarly journals Methodology for the Evaluation of the Electrical Energy Potential of Residual Biomass from the Wood Industry: A Case Study in Brazil

2021 ◽  
Author(s):  
Augusto César de Mendonça Brasil
Keyword(s):  
Power Plants ◽  
Real Data ◽  
Electrical Energy ◽  
Plant Size ◽  
The State ◽  
Wood Industry ◽  
Energy Potential ◽  
Curve Fit ◽  
Wood Residues

This chapter presents in a consolidated manner the step-by-step methodology to estimate the electrical energy potential of industrial wood residues considering the dependency of the efficiency of the power plants with their size. A function of the overall efficiency with power was obtained from a best curve fit of real data both taken from the literature and from Brazilian biomass-fired power plants. The methodology was applied to the determination of the electrical energy potential of wood industry residues in the State of Pará (data collected in 2004). Two cases were analyzed: one where a constant electrical efficiency of 25% was considered (independently of the amount of residues generated) and another where the proposed function of efficiency with power was used. Results show that in the State of Pará, the existent 675 sawmills generated 2.95 × 106 t in dry basis. When the dependency of efficiency with plant size is not considered, the electrical energy potential and average installed power (3140.4 GWh and 2 MWe) are overestimated in comparison to the herein proposed methodology (1868.8 GWh and 1 MWe). The present methodology, considering the efficiency as a function of the power, results in an average efficiency of 12.3% (lower than 25%).

scholarly journals Colchicine-induced chromosome doubling in Pennisetum interspecific hybrids and its effect on plant morphology

2020 ◽  
Vol 80 (01) ◽  
Author(s):  
Arshpreet Kaur ◽  
Rahul Kapoor ◽  
Yogesh Vikal ◽  
Anu Kalia
Keyword(s):  
Interspecific Hybrids ◽  
Biomass Yield ◽  
Chromosome Doubling ◽  
Plant Morphology ◽  
Colchicine Treatment ◽  
Stem Cuttings ◽  
Chromosome Counting ◽  
Stomatal Frequency ◽  
Preliminary Screening ◽  
Stomata Size

We report the production of hexaploid plants of interspecific hybrids of Pennisetum, with the ultimate aim to improve the biomass yield, drought tolerance and multicut behaviour of this genus. Chromosome doubling was achieved with the application of colchicine at three different concentrations (0.05, 0.1 and 0.2%) for two time durations (12 and 24 hours). The root slips and stem cuttings of interspecific hybrids were used for treatment and the root slips were found to be more efficient. The preliminary screening to select the putative hexaploid plant was done based on stomatal frequency and morphology. Plants containing significantly lower stomatal frequency and larger stomata size were selected for further analysis by chromosome counting. This experiment confirmed that 0.1% concentration of colchicine treatment to root slips for 24 hours was more effective to induce the amphiploids in Pennisetum.

scholarly journals THE MOST COMMON DISEASES OF ORNAMENTAL PLANTS IN THE ENTITY OF REPUBLIC OF SRPSKA (BOSNIA AND HERZEGOVINA)

AGROFOR ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Vojislav TRKULJA ◽  
Bojana ĆURKOVIĆ ◽  
Jelena VASIĆ ◽  
Bojana VUKOVIĆ ◽  
Gordana BABIĆ ◽  
...  
Keyword(s):  
Ornamental Plants ◽  
Plant Viruses ◽  
Plant Diseases ◽  
Impatiens Necrotic Spot Virus ◽  
Molecular Characteristics ◽  
Bacterial Diseases ◽  
Tomato Spotted Wilt ◽  
Alternaria Sp ◽  
Wilt Virus

During five year period (2011-2015) on territory of Republic of Srpska thecontinuous monitoring was carried out on different ornamental flowers for thepresence of plant diseases. For laboratory analysis following species Alyssum sp.,Aster sp., Bacopa sp., Begonia sp., Bellis perennis, Calceolaria sp.,Chrysanthemum sp., Cyclamen sp., Dahlia sp., Dianthus sp., Fuchsia sp., Gazaniasp., Gloxinia sp., Lobelia sp., Myosotis sp., Pelargonium sp., Petunia sp.,Petuniaxhybrida, Phlox sp., Plectranthus sp., Portulaca grandiflora, Primula sp.,Ranunculus sp., Rusmarinus officinalis, Salvia sp., Sansevaria sp., Tagetes sp.,Impatiens sp., Verbena sp. and Viola sp. were taken. Determination of causalpathogens was carried out on the basis of studying their morphological, pathogenic,biochemical and physiological, serological and molecular characteristics. The mostcommon causal pathogens were Alternaria sp., Botrytis sp., Golvonomyces sp.,Pestalotia sp., Phomopsis sp., Peronospora sp., Phytophtora sp., Puccinia sp. andSeptoria sp. Also, in much less extent, bacterial and viruses diseases were present.The most common bacterial diseases appear to be genera Pseudomonas andErwinia, while the most frequent plant viruses were Tomato spotted wilt virus(TSWV) and Impatiens necrotic spot virus (INSV).

scholarly journals Effects of Fertilization on the Growth and Quality of Container-grown Areca Palm and Chinese Hibiscus during Establishment in the Landscape

2010 ◽  
Vol 20 (2) ◽  
pp. 389-394
Author(s):  
Timothy K. Broschat ◽  
Kimberly Anne Moore
Keyword(s):  
Ornamental Plants ◽  
Plant Size ◽  
N Fertilization ◽  
N Application ◽  
Landscape Plants ◽  
Application Rates ◽  
Affected Plant ◽  
Highly Correlated ◽  
Chinese Hibiscus

The roots of container-grown ornamental plants primarily are concentrated within the original container substrate root ball during the establishment period following transplanting into the landscape. Plants growing in container substrates containing pine bark or peatmoss have higher nitrogen (N) requirements than in most landscape soils due to microbial immobilization of N by these organic components. However, use of high-N fertilizers, such as those used in container production of ornamentals, can cause imbalances with potassium (K) and magnesium (Mg) when used on palms in sandy landscape soils. Areca palm (Dypsis lutescens) and chinese hibiscus (Hibiscus rosa-sinensis ‘President’) that had been growing in containers were transplanted into a landscape soil to determine if high N fertilization during the establishment period could accelerate the rate of establishment without exacerbating K and Mg deficiencies. Although plants of both species had the darkest green color and largest size when continuously fertilized with high N fertilizer, this treatment did induce Mg deficiency in both species. Plant size and color for both species were highly correlated with cumulative N application rates, but also with initial N application rates, suggesting that high N fertilization during the first 6 months affected plant quality at 12 and 24 months after planting, even if high N fertilization was discontinued. However, continued use of a moderate N landscape palm maintenance fertilizer ultimately produced areca palm plants as good as those receiving high N during the establishment period.

scholarly journals 085 Horticultural (Cultivated Plant) Taxonomic Research within ASHS

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 456A-456
Author(s):  
Paul R. Fantz ◽  
Donglin Zhang
Keyword(s):  
Ornamental Plants ◽  
Sem Analysis ◽  
Cultivated Plants ◽  
Correct Identification ◽  
Breeding Programs ◽  
Green Industry ◽  
Code Of Nomenclature ◽  
Taxonomic Research ◽  
Cultivated Plant

Horticultural Science in the past quarter of a century has been shifting to increased emphasis on ornamental plants due to the growth of the modern green industry. Numerous species are being introduced into the exterior and interior landscapes. For popular species, the cultivar, as defined by the International Code of Nomenclature for Cultivated Plants (ICNCP), has become the basic taxon of cultivated plants. Named ornamental plant cultivars are rising at a rapid rate creating identification and segregation problems in the landscape industry, nurseries, botanic gardens, arboreta, and breeding programs. Government regulations and legal issues are beginning to infringe as solutions to the problems. There is a critical need existing for taxonomic research on ornamental cultivars utilizing classical morphological analysis supplemented with modern biotechnological techniques (e.g., anatomical, chemical, cytological, DNA, Sem analysis). Taxonomic research on existing and newer cultivars can provide quantitative botanical descriptions, keys of segregation, correct identification, determination of correct names and synonymy, improved cultivar documentation, and grouping of similar cultivars in large complexes. The taxonomic research is basic science that has immediate applied application within the horticultural society, and results should be published in the journals of ASHS.

scholarly journals Capture of Sprinkler Irrigation Water by Container-grown Ornamental Plants

HortScience ◽  
2015 ◽  
Vol 50 (3) ◽  
pp. 442-446 ◽  
Author(s):  
Jeff B. Million ◽  
Thomas H. Yeager
Keyword(s):  
Plant Species ◽  
Irrigation Water ◽  
Critical Role ◽  
Ornamental Plants ◽  
Sprinkler Irrigation ◽  
Dynamic Parameter ◽  
Plant Size ◽  
Ligustrum Japonicum ◽  
Container Nurseries ◽  
Size Container

The capacity for container-grown plants to capture sprinkler irrigation water plays a critical role in adjusting irrigation rates to deliver required amounts of water to the container substrate. The capture factor (CF) used to describe this capacity was defined as the amount of water captured with a plant relative to the amount captured without a plant. A wind-sheltered, irrigation test area was established to measure CF as affected by plant species, plant size, container size, container spacing, and sprinkler type. CF values for 11 marketable-sized, commonly grown plant species ranged from 1 to 4 with highest values exhibited by plant species with an upright, spreading growth habit. CF values increased as plant size increased. Close container spacings (less than one container diameter between adjacent containers) reduced CF when the allotted area outside the container limited the potential amount of water that could be captured. Compared with impact sprinklers, wobbler sprinklers increased irrigation capture 7% for Ligustrum japonicum grown in 27-cm-diameter containers but not in 16-cm-diameter containers. Results showed that CF is a dynamic parameter that depends on canopy size, container size, container spacing, and sprinkler type. A working knowledge of CF is crucial for determining irrigation requirements to maximize sprinkler irrigation efficiency in container nurseries.

scholarly journals REPRODUCTIVE BEHAVIOR OF DIPLOID AND ALLOTETRAPLOID AZALEODENDRONS

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 497E-498
Author(s):  
Ryan N. Contreras ◽  
Thomas G. Ranney
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Reproductive Behavior ◽  
Chromosome Doubling ◽  
Pollen Viability ◽  
Viable Pollen ◽  
Wide Hybrids ◽  
Viable Seeds ◽  
Cold Hardy ◽  
Mitotic Inhibitor

Wide hybridization can potentially lead to the combination of diverse traits, but these hybrids are often sterile as is the case with the inter-subgeneric hybrid Rhododendron `Fragrant Affinity'. Induction of polyploidy can restore chromosome homology and fertility in wide hybrids. In this study we successfully developed an allopolyploid form of R. `Fragrant Affinity' using oryzalin as a mitotic inhibitor and chromosome doubling agent. Approximate genome size (2C), determined using flow cytometry, was 1.6 pg for the diploid and 3.2 pg for the allotetraploid. Pollen viability, determined by staining and germination tests, was 4% and 0%, respectively for the diploid and 68% and 45%, respectively for the allotetraploid. No seeds were produced when the diploid R. `Fragrant Affinity' was crossed with pollen from viable diploid and tetraploid parents. The allotetraploid produced viable seeds and seedlings when crossed with viable pollen from either diploid or tetraploid parents, including self pollination, demonstrating restored fertility. Additional crosses were successfully completed using the allotetraploid as part of an ongoing breeding program to develop new fragrant, cold hardy, evergreen rhododendron.

scholarly journals FEATURES OF DETERMINATION OF THE COST OF PLANTS CERTAIN KINDS WHEN CARRYING OUT FORENSIC MERCHANDISING EXAMINATIONS

2017 ◽  
Vol 17 ◽  
pp. 351-359
Author(s):  
О. S. Obolentseva-Krasivska
Keyword(s):  
Ornamental Plants ◽  
Biological Properties ◽  
Planting Material ◽  
The Subject ◽  
Definition Of ◽  
The Cost ◽  
Market Relations

Flowers, ornamental plants, planting material (saplings) and other plants with regard to which the market with possibility of carrying out independent adequate marketing was formed, are the subject of evaluation by the experts in field of forensic merchandising. With the development of the market and market relations such goods as flowers, planting material and others are increasingly encashed in the market of Ukraine and become the objects of forensic merchandising examinations and expert researches. Special feature of merchandising researches of the plant origin objects is the use of the comprehensive approach to the estimation of their quality and cost. Researches are carried out with the purpose of determination of properties, consumer value, conformity to standards and specifications of plants certain kinds. When studying a considerable variety of flower plants, one distinguishes scientific and industrial classifications. In merchandising flowers industrial classification of flower ornamental plants which provides distribution of flower plants to the separate groups similar on biological properties, the agricultural technician of cultivation and practical application in gardening is used. During carrying out merchandising examinations on an establishment of consumer properties of certain kinds plants, namely establishments organoleptic indicators of quality and quantity of flower decorative production given for research, the expert checks conformity of this production to requirements of normative and technical documents. Requirements to quality of flower production are normalised by standards depending on production kind – cutf lowers, pottery blossoming plants, pottery decorative sheet plants, sprouts of flower decorative production, planting material, seeds of flower ornamental plants. At carrying out merchandising researches of flower decorative production, plants and saplings it’s necessary for merchandising experts to pay special attention on definition of quality indicators and a grade of plants according to standards, and also to consider data concerning age of a plant, novelty of a grade and origin country of production.

Comparison of Chlorophyll Fluorescence and Fresh Weight as Herbicide Bioassay Techniques

Weed Science ◽  
1985 ◽  
Vol 33 (1) ◽  
pp. 29-33 ◽  
Author(s):  
David R. Shaw ◽  
Thomas F. Peeper ◽  
D. L. Nofziger
Keyword(s):  
Chlorophyll Fluorescence ◽  
Avena Sativa ◽  
Inflection Point ◽  
Plant Size ◽  
Variable Fluorescence ◽  
Fresh Weight ◽  
Reliable Technique ◽  
Treated Soil ◽  
Fluorescence Maximum

The sensitivities of chlorophyll fluorescence and fresh weight as bioassay techniques for the determination of metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5 (4H)-one], diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], and atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] concentrations in soil were compared. The ratio of the initial inflection point (I) to the variable fluorescence maximum (P) of intact oat (Avena sativaL. ‘Chilocco’) leaves was determined for plants seeded directly into herbicide-treated soil and for those transplanted into treated soil after 14 days of growth in nutrient solution. Using the chlorophyll fluorescence of transplanted oats bioassay, 0-, 0.13-, 0.25-, and 0.50-ppm concentrations could be distinguished from one another within 8 h for metribuzin, within 24 h for diuron, and within 48 h for atrazine. These distinctions between rates could not be made 17 days after seeding into treated soil when using fresh weight as the bioassay indicator. Chlorophyll fluorescence of oats seeded directly into treated soil was also a reliable technique, but required much more time to attain sufficient plant size for convenient chlorophyll fluorescence determinations.

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