scholarly journals The Covalent Tethering of Poly(ethylene glycol) to Nylon 6 Surface via N,N′-Disuccinimidyl Carbonate Conjugation: A New Approach in the Fight against Pathogenic Bacteria

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2181
Author(s):  
Sumita Swar ◽  
Veronika Máková ◽  
Ivan Stibor
Keyword(s):  
Photoelectron Spectroscopy ◽  
Pathogenic Bacteria ◽  
Polymer Surface ◽  
Nylon 6 ◽  
Secondary Amines ◽  
Surface Immobilization ◽  
Water Contact ◽  
Force Microscopy ◽  
Poly Ethylene

Different forms of unmodified and modified Poly(ethylene glycols) (PEGs) are widely used as antifouling and antibacterial agents for biomedical industries and Nylon 6 is one of the polymers used for biomedical textiles. Our recent study focused on an efficient approach to PEG immobilization on a reduced Nylon 6 surface via N,N′–disuccinimidyl carbonate (DSC) conjugation. The conversion of amide functional groups to secondary amines on the Nylon 6 polymer surface was achieved by the reducing agent borane-tetrahydrofuran (BH3–THF) complex, before binding the PEG. Various techniques, including water contact angle and free surface energy measurements, atomic force microscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy, were used to confirm the desired surface immobilization. Our findings indicated that PEG may be efficiently tethered to the Nylon 6 surface via DSC, having an enormous future potential for antifouling biomedical materials. The bacterial adhesion performances against S. aureus and P. aeruginosa were examined. In vitro cytocompatibility was successfully tested on pure, reduced, and PEG immobilized samples.

scholarly journals Synthesis of amphiphilic ABA triblock oligomer via ATRP and its surface properties

2017 ◽  
Vol 95 (5) ◽  
pp. 605-611 ◽  
Author(s):  
Lei Wang ◽  
Shaoqing Wen ◽  
Zhanxiong Li
Keyword(s):  
Photoelectron Spectroscopy ◽  
Microphase Separation ◽  
Gel Permeation Chromatography ◽  
Contact Angles ◽  
Chemical Compositions ◽  
Water Contact ◽  
Force Microscopy ◽  
Atomic Force ◽  
Poly Ethylene ◽  
Magnetic Resonances

A series of novel amphiphilic ABA-type poly(tridecafluorooctylacrylate)-poly(ethylene glycol)-poly(tridecafluorooctylacrylate) (henceforth referred to as p-TDFA-PEG-p-TDFA) triblock oligomers were successfully synthesized via atom transfer radical polymerization (ATRP) using well-defined Br-PEG-Br as macroinitiator and copper as catalyst. The block oligomers were characterized by Fourier transform infrared (FTIR) spectroscopy and 1H and 19F nuclear magnetic resonances (NMR). Gel permeation chromatography (GPC) showed that the block oligomers have been obtained with narrow molecular weight distributions of 1.22–1.33. X-ray photoelectron spectroscopy (XPS) was carried out to confirm the attachment of p-TDFA-PEG-p-TDFA onto the silicon substrate, together with the chemical compositions of p-TDFA-PEG-p-TDFA. The wetabilities of the oligomer films were measured by water contact angles (CAs). Water CAs of p-TDFA-PEG-p-TDFA film were measured and their morphologies were tested by atomic force microscopy (AFM). The result showed that the CAs of the oligomer films, which possess fluoroalkyl groups assembled on the outer surface, increase after heating due to the migration of fluoroalkyl groups and the resulted microphase separation of the p-TDFA-PEG-p-TDFA.

Surface Modification of Polycarbonateurethane by Grafting Phosphorylcholine Glyceraldehydes for Improving Hemocompatibility

2012 ◽  
Vol 1403 ◽  
Author(s):  
Wei Gao ◽  
Yakai Feng ◽  
Jian Lu ◽  
Jintang Guo
Keyword(s):  
Contact Angle ◽  
Photoelectron Spectroscopy ◽  
Polymer Surface ◽  
Xps Analysis ◽  
Poly Ethylene Glycol ◽  
Water Contact ◽  
X Ray ◽  
Poly Ethylene ◽  
Covalently Linked ◽  
Scanning Electron

ABSTRACTPhosphorylcholine glyceraldehyde (PCGA) was used as a phosphorylcholine (PC) group containing compound to graft onto the surface of polycarbonateurethane (PCU) film using 1,6-hexanediamine (HDA) or α,ω-diamino-poly(ethylene glycol) (APEG, Mn = 200) as a spacer, in order to introduce biomimetic structure onto the polymer surface. X-ray photoelectron spectroscopy (XPS) analysis shows that PCGA has been covalently linked to the PCU surface. Water contact angle test suggests that the surface hydrophilicity has been improved after PCGA is grafted onto the surface of PCU film. Scanning electron microscope (SEM) observation of the modified PCU films after contacting with plasma-rich plasma demonstrates that platelets rarely adhere but a large number of platelets adhere to the original PCU surface. The hemocompatibility of the PC modified PCU film has been improved obviously after grafting with PCGA with PEG spacer.

Biomimetic Hemo-compatible Surfaces of Polyurethane by Grafting Copolymer Brushes of Poly(ethylene glycol) and Poly(phosphorylcholine methacrylate)

2012 ◽  
Vol 1403 ◽  
Author(s):  
Dazhi Yang ◽  
Yakai Feng ◽  
Marc Behl ◽  
Andreas Lendlein ◽  
Haiyang Zhao ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Photoelectron Spectroscopy ◽  
Platelet Adhesion ◽  
Extended Period ◽  
Phase Image ◽  
Poly Ethylene Glycol ◽  
Water Contact ◽  
Modified Surfaces ◽  
Poly Ethylene

ABSTRACTPolyurethanes (PU) have been widely used as biomaterial in recent years, while thrombus may still occur when contacting with blood especially for extended period of time. Poly(ethylene glycol) (PEG) and phosphorylcholine (PC)-based polymers are commonly employed for surface modification to create protein repellent surfaces. PC-based polymers have been investigated as biomimetic materials because PC is the major component in the outer layer of cell membranes. In this study, the biomimetic copolymer brush of PEG-b-poly(2-methacryloyloxyethyl phosphorylcholine) on PU surfaces was synthesized via atom transfer radical polymerization (ATRP) with a surface initiator. The flexible PEG chain was 200 g·mol-1, while the poly(2-methacryloyloxyethyl phosphorylcholine) (poly(MPC)) chain length was controlled by the ratio of monomer to sacrificial initiator in solution. The topology of the modified surfaces was characterized by the phase image of atomic force microscopy (AFM) to study the synergy effect between PEG chains and poly(MPC) chains. The unmodified and modified surfaces were characterized by Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), water contact angle and platelet adhesion. The results demonstrated that efficient grafting of PEG-b-poly(MPC) brushes on the surfaces was achieved. The PU surfaces modified with PEG and phosphorylcholine zwitterionic brushes showed effective resistance to platelet adhesion and high hemocompatibility in vitro. These PEG and PC-grafted PU materials might be potentially applied in blood-contacting materials or devices due to their good mechanical and hemocompatible properties.

scholarly journals In Vitro Corrosion Studies of Surface Modified NiTi Alloy for Biomedical Applications

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Manju Chembath ◽  
J. N. Balaraju ◽  
M. Sujata
Keyword(s):  
Contact Angle ◽  
Photoelectron Spectroscopy ◽  
Electrochemical Impedance ◽  
Niti Alloy ◽  
Passive Layer ◽  
Water Contact ◽  
Force Microscopy ◽  
Corrosion Studies ◽  
Surface Modified

Electropolishing was conducted on NiTi alloy of composition 49.1 Ti-50.9 Ni at.% under potentiostatic regime at ambient temperature using perchloric acid based electrolyte for 30 sec followed by passivation treatment in an inorganic electrolyte. The corrosion resistance and biocompatibility of the electropolished and passivated alloys were evaluated and compared with mechanically polished alloy. Various characterization techniques like scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy were employed to analyze the properties of surface modified and mechanically polished alloys. Water contact angle measurements made on the passivated alloy after electropolishing showed a contact angle of 35.6°, which was about 58% lower compared to mechanically polished sample, implying more hydrophilicity. The electrochemical impedance studies showed that, for the passivated alloy, threefold increase in the barrier layer resistance was obtained when compared to electropolished alloy due to the formation of compact titanium oxide. The oxide layer thickness of the passivated samples was almost 18 times higher than electropolished samples. After 14 days immersion in Hanks’ solution, the amount of nickel released was 315 ppb which was nearly half of that obtained for mechanically polished NiTi alloy, confirming better stability of the passive layer.

Surface Characterization and In Vitro Blood Compatibility of Poly(Ethylene Terephthalate) Immobilized with Hirudin

2005 ◽  
Vol 288-289 ◽  
pp. 421-424
Author(s):  
F. Li ◽  
Jin Wang ◽  
H. Sun ◽  
Nan Huang
Keyword(s):  
Free Energy ◽  
Photoelectron Spectroscopy ◽  
Surface Characterization ◽  
Ethylene Terephthalate ◽  
Blood Compatibility ◽  
Argon Plasma ◽  
Angle Measurement ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene

Poly(ethylene terephthalate) films were exposed under argon plasma glow discharge and induced polymerization of acrylic acid (AA) in order to introduce carboxylic acid group onto PET (PET-AA) assisting by ultroviolet radiation. Hirudin-immobilized PETs were prepared by the grafting of PET-AA, followed by chemical reaction with hirudin. The surface structure of the treated PET is determined by X-ray photoelectron spectroscopy (XPS). The wettability and surface free energy, interface free energy of the films is investigated by contact angle measurement. Platelet adhesion evaluatiion is conducted to examine the blood compatibility in vitro. Scanning electron microscopy (SEM) and optical microscopy reveal that the amounts of adhered, aggregated and morphologically changed platelets are reduced on hirudin-immobilized PET films.

Surface Modification of PEOT/PBT Membrane with Silk Fibroin Anchoring and its Potential Application in Artificial Salivary Gland Construct

2012 ◽  
Vol 538-541 ◽  
pp. 52-59
Author(s):  
Jie Zhu ◽  
Ming Shi Li ◽  
Li Qun Wang ◽  
Xiao Lin Zhu
Keyword(s):  
Salivary Gland ◽  
Silk Fibroin ◽  
Potential Application ◽  
Contact Angles ◽  
Water Contact ◽  
Polar Groups ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Surface Modified

We reported the preparation of surface modified poly (ethylene oxide terephthalate) - poly (butylene terephthalate) membrane by the method of silk fibroin anchoring, namely SF/(PEOT/PBT). Its surface properties were characterized by contact angles and XPS and the biocompatibility of the composite membrane was further evaluated by human salivary epithelial cells (HSG cells) growth in vitro. Results revealed that SF/(PEOT/PBT) possessed the low water contact angle (48.0±3.0°) and immobilized a great amount of fibroin (fibroin surface coverage: 26.39 wt%), which attributed to the formation of polar groups such as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. HSG cells growth in vitro indicated that the silk fibroin anchoring could significantly enhance the biocompatibility of PEOT/PBT membrane, which suggested the potential application of fibroin anchoring PEOT/PBT for clinical HSG cells transplantation in the artificial salivary gland construct.

scholarly journals Superhydrophilic Coating with Antibacterial and Oil-Repellent Properties via NaIO4-Triggered Polydopamine/Sulfobetaine Methacrylate Polymerization

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2008
Author(s):  
Hsiu-Wen Chien ◽  
Hong-Yu Lin ◽  
Chau-Yi Tsai ◽  
Tai-Yu Chen ◽  
Wei-Nian Chen
Keyword(s):  
Surface Modification ◽  
Photoelectron Spectroscopy ◽  
Deposition Process ◽  
Water Contact ◽  
Water Separation ◽  
Force Microscopy ◽  
Atomic Force ◽  
Oil Water Separation ◽  
One Step ◽  
Oil Repellent

Superhydrophilic coatings have been widely used for the surface modification of membranes or biomedical devices owing to their excellent antifouling properties. However, simplifying the modification processes of such materials remains challenging. In this study, we developed a simple and rapid one-step co-deposition process using an oxidant trigger to fabricate superhydrophilic surfaces based on dopamine chemistry with sulfobetaine methacrylate (SBMA). We studied the effect of different oxidants and SBMA concentrations on surface modification in detail using UV–VIS spectrophotometry, dynamic light scattering, atomic force microscopy, X-ray photoelectron spectroscopy, and surface plasmon resonance. We found that NaIO4 could trigger the rate of polymerization and the optimum ratio of dopamine to SBMA is 1:25 by weight. This makes the surface superhydrophilic (water contact angle < 10°) and antifouling. The superhydrophilic coating, when introduced to polyester membranes, showed great potential for oil/water separation. Our study provides a complete description of the simple and fast preparation of superhydrophilic coatings for surface modification based on mussel-inspired chemistry.

Electrically-Controlled Penicillin/Streptomycin Release from Nanostructured Polypyrrole Coated on Titanium for Orthopedic Implants

2009 ◽  
Vol 151 ◽  
pp. 197-202 ◽  
Author(s):  
Sirinrath Sirivisoot ◽  
Rajesh A. Pareta ◽  
Thomas J. Webster
Keyword(s):  
Photoelectron Spectroscopy ◽  
Orthopedic Implants ◽  
Nanometer Scale ◽  
Force Microscopy ◽  
Atomic Force ◽  
Osteoblast Adhesion ◽  
Antibiotic Release ◽  
Orthopedic Applications ◽  
Scale Roughness

Implant infection leading to revision surgery can be avoided by incorporating controllable antibiotic release from titanium (Ti) implant surfaces. In this study, penicillin/streptomycin (P/S) and dexamethasone (Dex) were successfully immobilized via electropolymerization within polypyrrole membranes coated on the surface of Ti, which is widely used in orthopedic applications. In vitro results showed that greater numbers of osteoblasts adhered on these polymer-coated substrates than on currently-used unmodified Ti. X-ray photoelectron spectroscopy was used to monitor and compare the reaction effectiveness and the yield of electropolymerization. Polypyrrole membranes conjugated with P/S and Dex, and then coated with PLGA, all possessed nanometer scale roughness, as analyzed by atomic force microscopy. In summary, this study demonstrated that drugs incorporated within electroactive polypyrrole membranes, whose release was controlled by applying voltages, supported osteoblast adhesion and could potentially fight bacterial infection.

A Facile Method for Construction of Antifouling Surfaces by Self-Assembled Polymeric Monolayers of PEG-Silane Copolymers Formed in Aqueous Medium

2006 ◽  
Vol 6 (11) ◽  
pp. 3507-3511
Author(s):  
Sangjin Park ◽  
Young Shik Chi ◽  
Insung S. Choi ◽  
Jiehyun Seong ◽  
Sangyong Jon
Keyword(s):  
Photoelectron Spectroscopy ◽  
Random Copolymers ◽  
Water Contact ◽  
Protein Resistance ◽  
Grafting Density ◽  
Model Protein ◽  
Monomer Feed ◽  
Self Assembled ◽  
Poly Ethylene ◽  
Nonspecific Protein Adsorption

Self-assembled polymeric monolayers (PMs) on Si/SiO2 wafers were prepared in water from a series of random copolymers of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and 3-(trimethoxysilyl)propyl methacrylate (TMSMA), denoted as poly(TMSMA-r-PEGMA). Four polymers of poly(TMSMA-r-PEGMA) were synthesized by free radical polymerization with a systematic variation of co-monomer feed ratios. Regardless of PEG grafting density in the copolymers, all PMs formed approximately 1 nm-thick film as measured by ellipsometry. However, the PMs with a higher grafting density of PEG resulted in more hydrophilic surfaces in terms of water contact angle. The protein resistance of the PMs was evaluated using bovine serum albumin (BSA) as a model protein. Analyses by ellipsometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) showed that the PMs of the copolymers markedly reduced the nonspecific adsorption of proteins compared to the unmodified Si/SiO2 wafers. The study also revealed that the PMs prepared from the copolymers with a higher PEG grafting density were more effective in resisting the nonspecific protein adsorption.

scholarly journals Effect of Sputtering Temperature on Fluorocarbon Films: Surface Nanostructure and Fluorine/Carbon Ratio

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 848 ◽  
Author(s):  
Qi Zhao ◽  
Feipeng Wang ◽  
Kaizheng Wang ◽  
Guibai Xie ◽  
Wanzhao Cui ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Target Material ◽  
Chemical Compositions ◽  
Water Contact ◽  
Force Microscopy ◽  
Fluorocarbon Films ◽  
Atomic Force ◽  
Surface Nanostructure ◽  
Deposited Film ◽  
Fluorocarbon Film

In this work, fluorocarbon film was deposited on silicon (P/100) substrate using polytetrafluoroethylene (PTFE) as target material at elevated sputtering temperature. Field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) were employed to investigate the surface morphology as well as structural and chemical compositions of the deposited film. The surface energy, as well as the polar and dispersion components, were determined by water contact angle (WCA) measurement. The experimental results indicated that increasing sputtering temperature effectively led to higher deposition rate, surface roughness and WCA of the film. It was found that the elevated temperature contributed to increasing saturated components (e.g., C–F2 and C–F3) and decreasing unsaturated components (e.g., C–C and C–CF), thus enhancing the fluorine-to-carbon (F/C) ratio. The results are expected aid in tailoring the design of fluorocarbon films for physicochemical properties.

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