A Bibliometric Analysis of the Global Trend of Using Alginate, Gelatine, and Hydroxyapatite for Bone Tissue Regeneration Applications

Collecting information from previous investigations and expressing it in a scientometrics study can be a priceless guide to getting a complete overview of a specific research area. The aim of this study is to explore the interrelated connection between alginate, gelatine, and hydroxyapatite within the scope of bone tissue and scaffold. A review of traditional literature with data mining procedures using bibliometric analyses was considered to identify the evolution of the selected research area between 2009 and 2019. Bibliometric methods and knowledge visualization technologies were implemented to investigate diverse publications based on the following indicators: year of publication, document type, language, country, institution, author, journal, keyword, and number of citations. An analysis using a bibliometric study found that 7446 papers were located with the keywords “bone tissue” and “scaffold”, and 1767 (alginate), 185 (gelatine), 5658 (hydroxyapatite) papers with those specific sub keywords. The number of publications that relate to “tissue engineering” and bone more than doubled between 2009 (1352) and 2019 (2839). China, the United States and India are the most productive countries, while Sichuan University and the Chinese Academy of Science from China are the most important institutions related to bone tissue scaffold. Materials Science and Engineering C is the most productive journal, followed by the Journal of Biomedical Materials Research Part A. This paper is a starting point, providing the first bibliometric analysis study of bone tissue and scaffold considering alginate, gelatine and hydroxyapatite. A bibliometric analysis would greatly assist in giving a scientific insight to support desired future research work, not only associated with bone tissue engineering applications. It is expected that the analysis of alginate, gelatine and hydroxyapatite in terms of 3D bioprinting, clinical outcomes, scaffold architecture, and the regenerative medicine approach will enhance the research into bone tissue engineering in the near future. Continued studies into these research fields are highly recommended.

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Dual Network Composites of Poly(vinyl alcohol)-Calcium Metaphosphate/Alginate with Osteogenic Ions for Bone Tissue Engineering in Oral and Maxillofacial Surgery

Bioengineering ◽

10.3390/bioengineering8080107 ◽

2021 ◽

Vol 8 (8) ◽

pp. 107

Author(s):

Lilis Iskandar ◽

Lucy DiSilvio ◽

Jonathan Acheson ◽

Sanjukta Deb

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Vinyl Alcohol ◽

Maxillofacial Surgery ◽

Bone Defects ◽

Bone Tissue Regeneration ◽

Poly Vinyl Alcohol ◽

Oral And Maxillofacial Surgery

Despite considerable advances in biomaterials-based bone tissue engineering technologies, autografts remain the gold standard for rehabilitating critical-sized bone defects in the oral and maxillofacial (OMF) region. A majority of advanced synthetic bone substitutes (SBS’s) have not transcended the pre-clinical stage due to inferior clinical performance and translational barriers, which include low scalability, high cost, regulatory restrictions, limited advanced facilities and human resources. The aim of this study is to develop clinically viable alternatives to address the challenges of bone tissue regeneration in the OMF region by developing ‘dual network composites’ (DNC’s) of calcium metaphosphate (CMP)—poly(vinyl alcohol) (PVA)/alginate with osteogenic ions: calcium, zinc and strontium. To fabricate DNC’s, single network composites of PVA/CMP with 10% (w/v) gelatine particles as porogen were developed using two freeze–thawing cycles and subsequently interpenetrated by guluronate-dominant sodium alginate and chelated with calcium, zinc or strontium ions. Physicochemical, compressive, water uptake, thermal, morphological and in vitro biological properties of DNC’s were characterised. The results demonstrated elastic 3D porous scaffolds resembling a ‘spongy bone’ with fluid absorbing capacity, easily sculptable to fit anatomically complex bone defects, biocompatible and osteoconductive in vitro, thus yielding potentially clinically viable for SBS alternatives in OMF surgery.

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3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

Polymers ◽

10.3390/polym12091962 ◽

2020 ◽

Vol 12 (9) ◽

pp. 1962 ◽

Cited By ~ 1

Author(s):

Abdullah M. Cakmak ◽

Semra Unal ◽

Ali Sahin ◽

Faik N. Oktar ◽

Mustafa Sengor ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bacterial Cellulose ◽

Bone Tissue ◽

Composite Scaffold ◽

Bone Tissue Regeneration ◽

Engineering Applications ◽

Bioactive Materials ◽

Degradation Rates

Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (~300 µm) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.

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Nanomaterial-based scaffolds for bone tissue engineering and regeneration

Nanomedicine ◽

10.2217/nnm-2020-0112 ◽

2020 ◽

Vol 15 (20) ◽

pp. 1995-2017

Author(s):

Guo Ye ◽

Fangyuan Bao ◽

Xianzhu Zhang ◽

Zhe Song ◽

Youguo Liao ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Tissue Regeneration ◽

Tissue Formation ◽

Topological Features ◽

Potential Risks ◽

Underlying Mechanisms ◽

Nanocomposite Scaffolds ◽

Bone Tissue Formation

The global incidence of bone tissue injuries has been increasing rapidly in recent years, making it imperative to develop suitable bone grafts for facilitating bone tissue regeneration. It has been demonstrated that nanomaterials/nanocomposites scaffolds can more effectively promote new bone tissue formation compared with micromaterials. This may be attributed to their nanoscaled structural and topological features that better mimic the physiological characteristics of natural bone tissue. In this review, we examined the current applications of various nanomaterial/nanocomposite scaffolds and different topological structures for bone tissue engineering, as well as the underlying mechanisms of regeneration. The potential risks and toxicity of nanomaterials will also be critically discussed. Finally, some considerations for the clinical applications of nanomaterials/nanocomposites scaffolds for bone tissue engineering are mentioned.

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Production Trends, Collaboration, and Main Topics of the Integrative and Complementary Oncology Research Area: A Bibliometric Analysis

Integrative Cancer Therapies ◽

10.1177/1534735419846401 ◽

2019 ◽

Vol 18 ◽

pp. 153473541984640 ◽

Cited By ~ 11

Author(s):

Jose A. Moral-Munoz ◽

Lidia Carballo-Costa ◽

Enrique Herrera-Viedma ◽

Manuel J. Cobo

Keyword(s):

Bibliometric Analysis ◽

The United States ◽

Research Area ◽

Current Status ◽

Future Research ◽

Nf Kappa B ◽

Republic Of China ◽

The Past ◽

Oncology Research ◽

Complementary Oncology

Background: The prevalence of cancer has increased over time worldwide. Nevertheless, the number of deaths has been reduced during the past 2 decades. Thus, one-third of the cancer patients are users of complementary and alternative therapies, looking for other types of interventions. The main aim of the present study is to understand the current status of the research in integrative and complementary oncology. Three different aspects were analyzed: production trends, country collaboration, and leading research topics. Methods: The dataset was obtained from the documents indexed under the Integrative and Complementary Medicine category of the Web of Science database from 1976 to 2017. VOSviewer and SciMAT software were employed to perform the bibliometric analysis. Results: The Journal of Ethnopharmacology, China Medical University and the People’s Republic of China are the leading producers in the field. Regarding the collaboration, the United States and China present a close connection. The scientific community is focused on the following topics: apoptosis, breast cancer, oxidative stress, chemotherapy, and nuclear factor-Kappa-B (NF-Kappa-B). Conclusions: The present article shows potentially important information that allows understanding of the past, present, and future of research in integrative and complementary oncology. It is a useful evidence-based framework on which to base future research actions and academic directions.

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PCL and PCL/PLA Scaffolds for Bone Tissue Regeneration

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.683.168 ◽

2013 ◽

Vol 683 ◽

pp. 168-171 ◽

Cited By ~ 2

Author(s):

Tatiana Patrício ◽

Antonio Gloria ◽

Paulo J. Bártolo

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Regeneration ◽

Cell Attachment ◽

Bone Tissue Regeneration ◽

Engineering Applications ◽

Printing System

This paper investigates the use of PCL and PCL/PLA scaffolds, produced using a novel additive biomanufacturing system called BioCell Printing, for bone tissue engineering applications. Results show that the BioCell Printing system produces scaffolds with regular and reproducible architecture, presenting no toxicity and enhancing cell attachment and proliferation. It was also possible to observe that the addition of PLA to PCL scaffolds strongly improves the biomechanical performance of the constructs.

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Bone Tissue Regeneration in the Oral and Maxillofacial Region: A Review on the Application of Stem Cells and New Strategies to Improve Vascularization

Stem Cells International ◽

10.1155/2019/6279721 ◽

2019 ◽

Vol 2019 ◽

pp. 1-15 ◽

Cited By ~ 8

Author(s):

Vivian Wu ◽

Marco N. Helder ◽

Nathalie Bravenboer ◽

Christiaan M. ten Bruggenkate ◽

Jianfeng Jin ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Regeneration ◽

Host Tissue ◽

Bone Tissue Regeneration ◽

Maxillofacial Region ◽

Oral And Maxillofacial Region ◽

New Strategies

Bone tissue engineering techniques are a promising alternative for the use of autologous bone grafts to reconstruct bone defects in the oral and maxillofacial region. However, for successful bone regeneration, adequate vascularization is a prerequisite. This review presents and discusses the application of stem cells and new strategies to improve vascularization, which may lead to feasible clinical applications. Multiple sources of stem cells have been investigated for bone tissue engineering. The stromal vascular fraction (SVF) of human adipose tissue is considered a promising single source for a heterogeneous population of essential cells with, amongst others, osteogenic and angiogenic potential. Enhanced vascularization of tissue-engineered grafts can be achieved by different mechanisms: vascular ingrowth directed from the surrounding host tissue to the implanted graft, vice versa, or concomitantly. Vascular ingrowth into the implanted graft can be enhanced by (i) optimizing the material properties of scaffolds and (ii) their bioactivation by incorporation of growth factors or cell seeding. Vascular ingrowth directed from the implanted graft towards the host tissue can be achieved by incorporating the graft with either (i) preformed microvascular networks or (ii) microvascular fragments (MF). The latter may have stimulating actions on both vascular ingrowth and outgrowth, since they contain angiogenic stem cells like SVF, as well as vascularized matrix fragments. Both adipose tissue-derived SVF and MF are cell sources with clinical feasibility due to their large quantities that can be harvested and applied in a one-step surgical procedure. During the past years, important advancements of stem cell application and vascularization in bone tissue regeneration have been made. The development of engineered in vitro 3D models mimicking the bone defect environment would facilitate new strategies in bone tissue engineering. Successful clinical application requires innovative future investigations enhancing vascularization.

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Transformed Cuttlefish Bone Scaffolds for Bone Tissue Engineering

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.89-91.47 ◽

2010 ◽

Vol 89-91 ◽

pp. 47-52 ◽

Cited By ~ 4

Author(s):

Elisa Battistella ◽

Silvia Mele ◽

S. Pietronave ◽

Ismaela Foltran ◽

G.I. Lesci ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Cell Attachment ◽

Engineering Application ◽

Surface Characteristics ◽

Bone Tissue Regeneration ◽

Tissue Engineering Application ◽

Bone Scaffolds ◽

Surface Development

Nature is full of many interesting things to work with, but many natural resources are also protected. In this view the recycling of aquaculture and fishery residues may lead to the manufacture of new devices and the isolation of new molecules with potential application in medicine. The aim of the present study was to explore the possibility to transform the cuttlefish bone into an hydroxyapatite scaffold suitable for bone tissue engineering application. The mixture of different lamellar porous structure of cuttlefish bone from the species Sepia Officinalis was selected and characterized, according to morphology (including porosity, surface development, surface characteristics) and mechanical properties. The material was transformed into suitable scaffold for bone tissue regeneration, trying to totally or partially convert calcium carbonate (aragonite) into calcium phosphate (hydroxyapatite HA) using hydrothermal transformation. The studies on cell attachment and proliferation (by MTT assay at different experimental times), cell morphology with Scanning Electron Microscopy (SEM), alkaline phosphatase (ALP) and osteocalcin (OC) activities and expressions by mouse osteoblast-like MC3T3-E1 cells on HA were investigated at different experimental times in cultures, in comparison with those observed on titanium specimens used as a control (ET and ST). Cell proliferation was less in HA transformed cuttlefish bone scaffolds than in ET and ST specimens. In contrast, good performance for osteoblasts differentiation was observed on HA transformed cuttlefish bone scaffolds, similar to those observed onto titanium scaffolds.

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Carbon Nanostructures in Bone Tissue Engineering

The Open Orthopaedics Journal ◽

10.2174/1874325001610010877 ◽

2016 ◽

Vol 10 (1) ◽

pp. 877-899 ◽

Cited By ~ 14

Author(s):

Brian Lee Perkins ◽

Naghmeh Naderi

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Regeneration ◽

Carbon Nanostructures ◽

Bone Tissue Regeneration ◽

Biophysical Properties ◽

Carbon Nanostructure ◽

Natural Bone ◽

Wide Range

Background:Recent advances in developing biocompatible materials for treating bone loss or defects have dramatically changed clinicians’ reconstructive armory. Current clinically available reconstructive options have certain advantages, but also several drawbacks that prevent them from gaining universal acceptance. A wide range of synthetic and natural biomaterials is being used to develop tissue-engineered bone. Many of these materials are currently in the clinical trial stage.Methods:A selective literature review was performed for carbon nanostructure composites in bone tissue engineering.Results:Incorporation of carbon nanostructures significantly improves the mechanical properties of various biomaterials to mimic that of natural bone. Recently, carbon-modified biomaterials for bone tissue engineering have been extensively investigated to potentially revolutionize biomaterials for bone regeneration.Conclusion:This review summarizes the chemical and biophysical properties of carbon nanostructures and discusses their functionality in bone tissue regeneration.

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Preparation and Evaluation of Biocompatible Composite for Bone Tissue Engineering

International Journal of Pharmacy and Biomedical Engineering ◽

10.14445/23942576/ijpbe-v4i3p102 ◽

2017 ◽

Vol 4 (3) ◽

pp. 7-14

Author(s):

Masud Rana Md. ◽

Naznin Akhtar ◽

Zahid Hasan Md. ◽

Asaduzzaman S M

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biomedical Application ◽

Bone Tissue Regeneration ◽

Precipitation Method ◽

Tissue Engineering Scaffolds ◽

Fixed Amount ◽

Co Precipitation

Bone tissue engineering with cells and synthetic extracellular matrix represents a new approach for the regeneration of mineralized tissue compared with the transplantation of bone. Hydroxyapatite (HA) and its composite with biopolymer are extensively developed and applied in bone tissue regeneration. The main aim of this study was to fabricate and characterize of HA apatite based biocompatible scaffold for bone tissue engineering. Scaffolds with different ratio of polymers (chitosan & alginate), and fixed amount of synthetic HA were prepared using in situ co precipitation method and mineral to polymer ratio was 1:1 to 1: 2 . A cross linker agent, 2-Hydroxylmethacrylate (HEMA) was added at different percentage (0.5-2%) into the selected composition and irradiated at 5- 25 kGy to optimize the proper mixing of components at the presence of HEMA. Fabricated scaffolds were analyzed to determine porosity, density, biodegradability, morphology and structural properties. Porosity and density of the prepared scaffold were 75 to 92% and 0.21 to 0.42 g/cm3 respectively. However, the swelling ratio of the fabricated scaffolds was ranged from 133 to 197%. Nonetheless, there had a reasonable in-vitro degradation of prepared scaffolds. Flourier transform infrared spectroscopy (FTIR) analysis showed intermolecular interaction between components in the scaffold. Pore size of scaffold was measured by scanning electron microscope and the value was 162-510 μm. It could be proposed that this scaffold fulfills all the main requirements to be considered as a bone substitute for biomedical application in near future.

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Mesoporous Silica Based Nanostructures for Bone Tissue Regeneration

Frontiers in Materials ◽

10.3389/fmats.2021.692309 ◽

2021 ◽

Vol 8 ◽

Author(s):

Sougata Ghosh ◽

Thomas J. Webster

Keyword(s):

Tissue Engineering ◽

Mesoporous Silica ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Regeneration ◽

Mesoporous Silica Nanoparticles ◽

Fluorescein Isothiocyanate ◽

Bone Tissue Regeneration ◽

Surface Modified ◽

Remarkable Progress

Porous nano-scaffolds provide for better opportunities to restore, maintain, and improve functions of damaged tissues and organs by facilitating tissue regeneration. Various nanohybrids composed of mesoporous silica nanoparticles (MSNs) are being widely explored for tissue engineering. Since biological activity is enhanced by several orders of magnitude in multicomponent scaffolds, remarkable progress has been observed in this field, which has aimed to develop the controlled synthesis of multifunctional MSNs with tuneable pore size, efficient delivering capacity of bioactive factors, as well as enhanced biocompatibility and biodegradability. In this review, we aim to provide a broad survey of the synthesis of multifunctional MSN based nanostructures with exotic shapes and sizes. Further, their promise as a novel nanomedicine is also elaborated with respect to their role in bone tissue engineering. Also, recent progress in surface modification and functionalization with various polymers like poly (l-lactic acid)/poly (ε-caprolactone), polylysine-modified polyethylenimine, poly (lactic-co-glycolic acid), and poly (citrate-siloxane) and biological polymers like alginate, chitosan, and gelatine are also covered. Several attempts for conjugating drugs like dexamethasone and β–estradiol, antibiotics like vancomycin and levofloxaci, and imaging agents like fluorescein isothiocyanate and gadolinium, on the surface modified MSNs are also covered. Finally, the scope of developing orthopaedic implants and potential trends in 3D bioprinting applications of MSNs are also discussed. Hence, MSNs based nanomaterials may serve as improved candidate biotemplates or scaffolds for numerous bone tissue engineering, drug delivery and imaging applications deserving our full attention now.

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