scholarly journals Development of Antibacterial Protective Coatings Active against MSSA and MRSA on Biodegradable Polymers

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 659
Author(s):  
Iva Rezić ◽  
Mislav Majdak ◽  
Vanja Ljoljić Bilić ◽  
Ivan Pokrovac ◽  
Lela Martinaga ◽  
...  

In this work the in vitro antimicrobial activity of colloidal solutions of nine different commercially available nanoparticles were investigated against Staphylococcus aureus strains, both methicillin-sensitive (MSSA) and methicillin-resistant (MRSA). Research covered antimicrobial investigation of different metal and metal-oxide nanoparticles, including Ag 10 nm, Ag 40 nm, Al2O3 100 nm, Au 20 nm, Pt 4 nm, TiO2 100 nm, Y2O3 100 nm, ZnO 100 nm and ZrO2 100 nm nanoparticles. Such materials were foreseen to be applied as coatings on 3D-printed biodegradable polymers: i.e., catheters, disposable materials, hospital bedding items, disposable antimicrobial linings and bandages for chronic wounds. Therefore, the antimicrobial activity of the nanoparticles was determined by agar well diffusion assays and serial microdilution broth assays. In addition, the chromatographic characterization of elements present in trace amounts was performed as a method for tracing the nanoparticles. Moreover, the potential of preparing the rough surface of biodegradable polymers for coating with antimicrobial nanoparticles was tested by 3D-printing fused deposition methodology. The in vitro results have shown that particular nanoparticles provided powerful antimicrobial effects against MSSA and MRSA strains, and can be easily applied on different biopolymers.

2017 ◽  
Vol 13 ◽  
pp. 239-247 ◽  
Author(s):  
Georgy A. Frolov ◽  
Yakov N. Karasenkov ◽  
Alexander A. Gusev ◽  
Olga V. Zakharova ◽  
Anna Yu. Godymchuk ◽  
...  

Preparation of nontoxic antimicrobial solutions is a priority in the development of new antibiotics. The in vitro effect of the chemical composition of the dispersed phase and dissolved solids in the dispersion medium on the antimicrobial properties of aqueous colloidal solutions of nanoparticles of a number of metals and metal oxides against microorganisms of different species - bacterial dental plaque, Bacillus cereus, and spores of vaccine strain Bacillus anthracis STI - 1 were studied in this work.


2021 ◽  
Vol 11 (23) ◽  
pp. 11526
Author(s):  
Florina Miere (Groza) ◽  
Alin Cristian Teușdea ◽  
Vasile Laslo ◽  
Simona Cavalu ◽  
Luminița Fritea ◽  
...  

The healing of skin wounds remains an important concern in medicine, especially in chronic wounds caused by various diseases such as diabetes. Using herbs or herbal products to heal skin wounds is a therapeutic challenge for traditional medicine. In this context, the main aim of our work was to highlight the in vitro healing potential of Stellaria media (L.) Vill. (SM) extract using the scratch assay on normal human dermal fibroblasts (NHDF). The ability to stimulate cell migration and proliferation under the influence of different concentrations of SM extract (range between 12.5 and 200 µg/mL) was determined compared to the control (untreated in vitro-simulated wound) and positive control (allantoin 50 µg/mL). Our results showed that the concentration of 100 µg/mL SM extract applied on the simulated wound recorded the strongest and fastest (24 h) migration (with wound closure) and proliferation of NHDF compared with the control. In addition, the SM extract was characterized in terms of bioactive compounds (total phenols and flavonoids content), antioxidant capacity (FRAP (The Ferric-Reducing Antioxidant Power) assay and electrochemical method), and antimicrobial activity. The results show that the SM extract contains a considerable amount of polyphenols (17.19 ± 1.32 mg GAE/g dw and 7.28 ± 1.18 mg QE/g dw for total phenol and flavonoid content, respectively) with antioxidant capacity. Antimicrobial activity against Gram-positive bacteria (S. aureus) is higher than E. coli at a dose of 15 µg/mL. This study showed that Stellaria media is a source of polyphenols compounds with antioxidant capacity, and for the first time, its wound healing potential was emphasized.


2021 ◽  
Vol 22 (8) ◽  
pp. 3996
Author(s):  
Karolina Dydak ◽  
Adam Junka ◽  
Agata Dydak ◽  
Malwina Brożyna ◽  
Justyna Paleczny ◽  
...  

Local administration of antiseptics is required to prevent and fight against biofilm-based infections of chronic wounds. One of the methods used for delivering antiseptics to infected wounds is the application of dressings chemisorbed with antimicrobials. Dressings made of bacterial cellulose (BC) display several features, making them suitable for such a purpose. This work aimed to compare the activity of commonly used antiseptic molecules: octenidine, polyhexanide, povidone-iodine, chlorhexidine, ethacridine lactate, and hypochlorous solutions and to evaluate their usefulness as active substances of BC dressings against 48 bacterial strains (8 species) and 6 yeast strains (1 species). A silver dressing was applied as a control material of proven antimicrobial activity. The methodology applied included the assessment of minimal inhibitory concentrations (MIC) and minimal biofilm eradication concentration (MBEC), the modified disc-diffusion method, and the modified antibiofilm dressing activity measurement (A.D.A.M.) method. While in 96-well plate-based methods (MIC and MBEC assessment), the highest antimicrobial activity was recorded for chlorhexidine, in the modified disc-diffusion method and in the modified A.D.A.M test, povidone-iodine performed the best. In an in vitro setting simulating chronic wound conditions, BC dressings chemisorbed with polyhexanide, octenidine, or povidone-iodine displayed a similar or even higher antibiofilm activity than the control dressing containing silver molecules. If translated into clinical conditions, the obtained results suggest high applicability of BC dressings chemisorbed with antiseptics to eradicate biofilm from chronic wounds.


2020 ◽  
Vol 36 (6) ◽  
pp. 1043-1048
Author(s):  
Goutam Kumar ◽  
P.P. Badoni ◽  
Arun K. Khajuria ◽  
Mahender Singh ◽  
Sapna Tyagi ◽  
...  

In recent years, there is significant increase in the attention towards the green synthesis of metal oxide nanoparticles, particularly by the interaction of plant extracts and metal salts; still there is no such published evidence is available on the synthesis of ZnO nanoparticles from aqueous leaf extract of Cotoneaster acuminatus. The synthesis of ZnO nanoparticles was confirmed by using several spectroscopic techniques i.e., UV-Vis, FT-IR, powder-XRD and TEM. It was confirmed from the spectroscopic data that the synthesized nanoparticles were hexagonally orientated, size in the 16-38 nm range and encapsulated by biochemicals present in the leaf extract. These nanoparticles were further evaluated for antimicrobial activity Bacillus subtilisNCFT.583.08, Staphylococcus aureusNCFT.576.08, Pseudomonas aeruginosaNCFT.645.11, Candida albicansNCFT.1006.11 and Saccharomyces cerevisiae NCFT.1008.11strains. This research work might be considered as a successful attempt to create and evaluate medicinal properties of ZnO nanoparticles in combination with Cotoneaster acuminatus leaf extract.


2019 ◽  
Author(s):  
Julian-Dario Rembe ◽  
Vivian-Denise Thompson ◽  
Nina Hauer ◽  
Ewa Klara Klara Stuermer

Abstract Background: Due to rising numbers microbial resistance to established antibiotics and first described tolerance developments for local wound antimicrobials a continuous need for alternative antimicrobial agents exists. Due to complex conditions in the microenvironment of especially chronic wounds, such as high protein levels, novel antimicrobials need to meet advanced requirements. Aim: Compare the antimicrobial efficacy of Cetylpiridinium-chloride (CPC) and miramistin (MST) to established antimicrobials under protein-challenge in-vitro. Methods: Antimicrobial activity of octenidin-dihydrochloride, povidon-iodine, polyhexamethylene-biguanide hydrochloride, chlorhexidine, cetylpiridinium-chloride and Miramistin after 0.5, 1, 3, 5 and 10 min of exposure against S. aureus, P. aeruginosa, E. coli, E. faecium and C. albicans was tested, using a quantitative suspension method with either 0.3% or 3% bovine albumin challenge, based on DIN EN 13727 (‘Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity in the medical area - Test method and requirements (phase 2, step 1)’). Results: CPC and MST demonstrated no inferiority to the established agents in-vitro. Especially CPC showed equal reduction rates as octenidin and povidon-iodine and achieved significantly higher reduction rates within shorter exposure times than polyhexanide and chlorhexidine (p ≤ 0.01) for S. aureus, P. aeruginosa, E. faecium and C. albicans. Both agents demonstrated no significant loss of efficacy under high protein-challenge (3% albumin). Conclusion: In terms of antimicrobial activity cetylpyridinium-chloride and miramistin proved to be at least equally effective as established agents. No protein error was detected in the tested concentrations. More complex in-vitro assays and comprehensive in-vivo and clinical studies will be needed to determine their clinical value.


2014 ◽  
Vol 7 ◽  
pp. MBI.S13914 ◽  
Author(s):  
Purushottam V. Gawande ◽  
Allie P. Clinton ◽  
Karen LoVetri ◽  
Nandadeva Yakandawala ◽  
Kendra P. Rumbaugh ◽  
...  

Chronic wounds including diabetic foot ulcers, pressure ulcers, and venous leg ulcers are a worldwide health problem. As the traditional methods of treatment have proven ineffective against chronic wounds involving biofilms, there is an unmet clinical need for developing products with an antibiofilm component that inhibits and/or disrupts biofilms and thus make the biofilm-embedded bacteria more susceptible to antimicrobial therapy. We developed a DispersinB® antibiofilm enzyme-based wound spray for treating chronic wounds in conjunction with an antimicrobial. Under in vitro conditions, the DispersinB® and Acticoat™ combination performed significantly better ( P < 0.05) than Acticoat™ alone, indicating the synergy between the two compounds because of DispersinB® enhancing the antimicrobial activity of Acticoat™. Furthermore, DispersinB® wound spray enhanced the antimicrobial activity of Acticoat™ in a chronic wound mouse model of methicillin-resistant Staphylococcus aureus (MRSA) infection. Thus, this novel combination of DispersinB® and Acticoat™, an antimicrobial dressing, prompts clinical evaluation for potential applications in biofilm-based chronic wound management.


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