scholarly journals Viscoelasticity, Mechanical Properties, and In Vitro Bioactivity of Gelatin/Borosilicate Bioactive Glass Nanocomposite Hydrogels as Potential Scaffolds for Bone Regeneration

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2014
Author(s):  
Asmaa M. Abd El-Aziz ◽  
Ahmed Abd El-Fattah ◽  
Azza El-Maghraby ◽  
Doaa A. Ghareeb ◽  
Sherif Kandil
Keyword(s):  
Mechanical Properties ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Loss Modulus ◽  
Apatite Formation ◽  
Sem Images ◽  
Nanocomposite Hydrogels ◽  
Nanocomposite Scaffolds ◽  
Different Content

Chemical cross-linking was used to create nanocomposite hydrogels made up of gelatin (G) and borosilicate bioactive glass (BBG) with different content (0, 3, and 5 wt.%). The G/BBG nanocomposite hydrogels were studied for their morphology, mechanical properties, and viscoelasticity. SEM images revealed a macroporous interconnected structure with particles scattered across the pore walls. Studies of water absorption and degradation confirmed that the nanocomposite scaffolds were hydrophilic and biodegradable. The addition of 5% BBG to the scaffold formulations increased the compressive modulus by 413% and the compressive intensity by 20%, respectively. At all frequency ranges tested, the storage modulus (G′) was greater than the loss modulus (G″), revealing a self-standing elastic nanocomposite hydrogel. The nanocomposite scaffolds facilitated apatite formation while immersed in simulated body fluid (SBF). According to the findings, G/BBG nanocomposite scaffolds could be a promising biomaterial for bone regeneration.

Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses

2019 ◽  
Vol 132 ◽  
pp. 811-821 ◽  
Author(s):  
Cheisy D.F. Moreira ◽  
Sandhra M. Carvalho ◽  
Rodrigo M. Florentino ◽  
Andressa França ◽  
Bárbara S. Okano ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Bone Regeneration ◽  
Nanocomposite Hydrogels ◽  
Regeneration In Vitro

scholarly journals Quenched/unquenched nano bioactive glass-ceramics: Synthesis and in vitro bioactivity evaluation in Ringer’s solution with BSA

2013 ◽  
Vol 19 (2) ◽  
pp. 231-239 ◽  
Author(s):  
Nima Nabian ◽  
Maedeh Delavar ◽  
Mahmood Rabiee ◽  
Mohsen Jahanshahi
Keyword(s):  
Electron Microscope ◽  
Bioactive Glass ◽  
Glass Ceramic ◽  
Sol Gel ◽  
Glass Ceramics ◽  
Apatite Formation ◽  
In Vitro Bioactivity ◽  
Sem Images ◽  
Transmission Electron

The paper reports the first attempt at changing cooling treatment of synthesizing method in order to investigate its effect on the physical properties of sol-gel derived nano bioactive glass-ceramic in the system 58SiO2-33CaO-9P2O5 (wt.%). We hypothesized that the method of cooling may affect the properties of nano bioactive glass-ceramic. To test this hypothesis, two different method of cooling treatment was applied after calcinations in synthesizing method. Both quenched and unquenched nano bioactive glass-ceramics were soaked in Ringer?s solution with bovine serum albumin (BSA) for bioactivity evaluation. The obtained samples were analyzed for their composition, crystalinity and morphology through X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), surface electron microscope (SEM) and transmission electron microscope (TEM). The SEM images showed that the morphology of nano bioactive glass-ceramics was completely changed by quenching process. Results of in vitro bioactivity evaluation revealed that the unquenched attains faster apatite formation ability than the quenched sample. Other properties of these two morphologically different nano bioactive glass-ceramics were strongly discussed.

scholarly journals Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1319
Author(s):  
Muhammad Umar Aslam Khan ◽  
Wafa Shamsan Al-Arjan ◽  
Mona Saad Binkadem ◽  
Hassan Mehboob ◽  
Adnan Haider ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Guar Gum ◽  
Porous Scaffolds ◽  
Vitro Assay ◽  
Vitro Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

scholarly journals Zn-Containing Membranes for Guided Bone Regeneration in Dentistry

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1797
Author(s):  
Manuel Toledano ◽  
Marta Vallecillo-Rivas ◽  
María T. Osorio ◽  
Esther Muñoz-Soto ◽  
Manuel Toledano-Osorio ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bone Healing ◽  
Bone Repair ◽  
Complex Modulus ◽  
Bacterial Colonization ◽  
Guided Bone Regeneration ◽  
M2 Macrophage

Barrier membranes are employed in guided bone regeneration (GBR) to facilitate bone in-growth. A bioactive and biomimetic Zn-doped membrane with the ability to participate in bone healing and regeneration is necessary. The aim of the present study is to state the effect of doping the membranes for GBR with zinc compounds in the improvement of bone regeneration. A literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. A narrative exploratory review was undertaken, focusing on the antibacterial effects, physicochemical and biological properties of Zn-loaded membranes. Bioactivity, bone formation and cytotoxicity were analyzed. Microstructure and mechanical properties of these membranes were also determined. Zn-doped membranes have inhibited in vivo and in vitro bacterial colonization. Zn-alloy and Zn-doped membranes attained good biocompatibility and were found to be non-toxic to cells. The Zn-doped matrices showed feasible mechanical properties, such as flexibility, strength, complex modulus and tan delta. Zn incorporation in polymeric membranes provided the highest regenerative efficiency for bone healing in experimental animals, potentiating osteogenesis, angiogenesis, biological activity and a balanced remodeling. Zn-loaded membranes doped with SiO2 nanoparticles have performed as bioactive modulators provoking an M2 macrophage increase and are a potential biomaterial for promoting bone repair. Zn-doped membranes have promoted pro-healing phenotypes.

scholarly journals Assessment of Titanate Nanolayers in Terms of Their Physicochemical and Biological Properties

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 806
Author(s):  
Michalina Ehlert ◽  
Aleksandra Radtke ◽  
Katarzyna Roszek ◽  
Tomasz Jędrzejewski ◽  
Piotr Piszczek
Keyword(s):  
Surface Modification ◽  
Substrate Surface ◽  
Biological Properties ◽  
Structure Characterization ◽  
Porous Coating ◽  
Apatite Formation ◽  
Sem Images ◽  
Energy Dispersion Spectroscopy ◽  
X Ray

The surface modification of titanium substrates and its alloys in order to improve their osseointegration properties is one of widely studied issues related to the design and production of modern orthopedic and dental implants. In this paper, we discuss the results concerning Ti6Al4V substrate surface modification by (a) alkaline treatment with a 7 M NaOH solution, and (b) production of a porous coating (anodic oxidation with the use of potential U = 5 V) and then treating its surface in the abovementioned alkaline solution. We compared the apatite-forming ability of unmodified and surface-modified titanium alloy in simulated body fluid (SBF) for 1–4 weeks. Analysis of the X-ray diffraction patterns of synthesized coatings allowed their structure characterization before and after immersing in SBF. The obtained nanolayers were studied using Raman spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy (DRIFT), and scanning electron microscopy (SEM) images. Elemental analysis was carried out using X-ray energy dispersion spectroscopy (SEM EDX). Wettability and biointegration activity (on the basis of the degree of integration of MG-63 osteoblast-like cells, L929 fibroblasts, and adipose-derived mesenchymal stem cells cultured in vitro on the sample surface) were also evaluated. The obtained results proved that the surfaces of Ti6Al4V and Ti6Al4V covered by TiO2 nanoporous coatings, which were modified by titanate layers, promote apatite formation in the environment of body fluids and possess optimal biointegration properties for fibroblasts and osteoblasts.

scholarly journals Development of 3D Bioactive Nanocomposite Scaffolds Made from Gelatin and Nano Bioactive Glass for Biomedical Applications

2010 ◽  
Vol 19 (2) ◽  
pp. 096369351001900 ◽  
Author(s):  
M. Mozafari ◽  
F. Moztarzadeh ◽  
M. Rabiee ◽  
M. Azami ◽  
N. Nezafati ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Biomedical Applications ◽  
Cell Attachment ◽  
Tissue Engineering Scaffolds ◽  
Study Results ◽  
Nanocomposite Scaffold ◽  
Nanocomposite Scaffolds ◽  
First Time

In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fabricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting combined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 μm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaffolds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.

scholarly journals Zinc-containing bioactive glasses for bone regeneration, dental and orthopedic applications

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Preethi Balasubramanian ◽  
Leonie A. Strobel ◽  
Ulrich Kneser ◽  
Aldo R. Boccaccini
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Human Body ◽  
Relevant Literature ◽  
The Body ◽  
Biological Behavior ◽  
Bioactive Glasses ◽  
Immune System Cell

AbstractZinc is a vital and beneficial trace element found in the human body. Though found in small proportions, zinc performs a variety of functions in relation to the immune system, cell division, fertility and the body growth and maintenance. In particular, zinc is proven to be a necessary element for the formation, mineralization, development and maintenance of healthy bones. Considering this attractive attributes of zinc, recent research has widely focused on using zinc along with silicate-based bioactive glasses for bone tissue engineering applications. This paper reviews relevant literature discussing the significance of zinc in the human body, along with its ability to enhance antibacterial effects, bioactivity and distinct physical, structural and mechanical properties of bioactive glasses. In this context, even if the present analysis is not meant to be exhaustive and only representative studies are discussed, literature results confirm that it is essential to understand the properties of zinc-containing bioactive glasses with respect to their in vitro biological behavior, possible cytotoxic effects and degradation characteristics to be able to effectively apply these glasses in bone regeneration strategies. Topics attracting increasing research efforts in this field are elaborated in detail in this review, including a summary of the structural, physical, biological and mechanical properties of zinc-containing bioactive glasses. This paper also presents an overview of the various applications in which zinc-containing bioactive glasses are considered for use as bone tissue scaffolds, bone filling granules, bioactive coatings and bone cements, and advances and remaining challenges are highlighted.

scholarly journals Comparison of the Effect of Sol-Gel and Coprecipitation Routes on the Properties and Behavior of Nanocomposite Chitosan-Bioactive Glass Membranes for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Elke M. F. Lemos ◽  
Sandhra M. Carvalho ◽  
Patrícia S. O. Patrício ◽  
Claudio L. Donnici ◽  
Marivalda M. Pereira
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Sol Gel ◽  
Composite Films ◽  
Maximum Tensile Stress ◽  
Control Group ◽  
Nanoparticle Dispersion

Recent studies in tissue engineering have highlighted the importance of the development of composite materials based on biodegradable polymers containing bioactive glasses, in particular, composites for high load support and excellent cell viability for potential application in bone regeneration. In this work, hybrid composite films were obtained by combining chitosan with bioactive glass in solution form and in nanoparticle dispersion form obtained by the two different synthesis routes: the sol-gel method and coprecipitation. The bioactive glass served both as a mechanical reinforcing agent and as a triggering agent with high bioactivity. The results ofin vitroassays with simulated body fluid demonstrated the formation of a significant layer of fibrils on the surface of the film, with a typical morphology of carbonated hydroxyapatite, reflecting induction of a favorable bioactivity. Maximum tensile stress increased from 42 to 80 MPa to the sample with 5% wt bioactive glass. In addition, samples containing 5% and 10% wt bioactive glass showed a significant increase in cell viability, 18 and 30% increase compared to the control group. The samples showed significant response, indicating that they could be a potential material for use in bone regeneration through tissue engineering.

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