Fibrous Polymer-Based Composites Obtained by Electrospinning for Bone Tissue Engineering

Currently, the significantly developing fields of tissue engineering related to the fabrication of polymer-based materials that possess microenvironments suitable to provide cell attachment and promote cell differentiation and proliferation involve various materials and approaches. Biomimicking approach in tissue engineering is aimed at the development of a highly biocompatible and bioactive material that would most accurately imitate the structural features of the native extracellular matrix consisting of specially arranged fibrous constructions. For this reason, the present research is devoted to the discussion of promising fibrous materials for bone tissue regeneration obtained by electrospinning techniques. In this brief review, we focus on the recently presented natural and synthetic polymers, as well as their combinations with each other and with bioactive inorganic incorporations in order to form composite electrospun scaffolds. The application of several electrospinning techniques in relation to a number of polymers is touched upon. Additionally, the efficiency of nanofibrous composite materials intended for use in bone tissue engineering is discussed based on biological activity and physiochemical characteristics.

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PCL and PCL/PLA Scaffolds for Bone Tissue Regeneration

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.683.168 ◽

2013 ◽

Vol 683 ◽

pp. 168-171 ◽

Cited By ~ 2

Author(s):

Tatiana Patrício ◽

Antonio Gloria ◽

Paulo J. Bártolo

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Regeneration ◽

Cell Attachment ◽

Bone Tissue Regeneration ◽

Engineering Applications ◽

Printing System

This paper investigates the use of PCL and PCL/PLA scaffolds, produced using a novel additive biomanufacturing system called BioCell Printing, for bone tissue engineering applications. Results show that the BioCell Printing system produces scaffolds with regular and reproducible architecture, presenting no toxicity and enhancing cell attachment and proliferation. It was also possible to observe that the addition of PLA to PCL scaffolds strongly improves the biomechanical performance of the constructs.

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Transformed Cuttlefish Bone Scaffolds for Bone Tissue Engineering

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.89-91.47 ◽

2010 ◽

Vol 89-91 ◽

pp. 47-52 ◽

Cited By ~ 4

Author(s):

Elisa Battistella ◽

Silvia Mele ◽

S. Pietronave ◽

Ismaela Foltran ◽

G.I. Lesci ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Cell Attachment ◽

Engineering Application ◽

Surface Characteristics ◽

Bone Tissue Regeneration ◽

Tissue Engineering Application ◽

Bone Scaffolds ◽

Surface Development

Nature is full of many interesting things to work with, but many natural resources are also protected. In this view the recycling of aquaculture and fishery residues may lead to the manufacture of new devices and the isolation of new molecules with potential application in medicine. The aim of the present study was to explore the possibility to transform the cuttlefish bone into an hydroxyapatite scaffold suitable for bone tissue engineering application. The mixture of different lamellar porous structure of cuttlefish bone from the species Sepia Officinalis was selected and characterized, according to morphology (including porosity, surface development, surface characteristics) and mechanical properties. The material was transformed into suitable scaffold for bone tissue regeneration, trying to totally or partially convert calcium carbonate (aragonite) into calcium phosphate (hydroxyapatite HA) using hydrothermal transformation. The studies on cell attachment and proliferation (by MTT assay at different experimental times), cell morphology with Scanning Electron Microscopy (SEM), alkaline phosphatase (ALP) and osteocalcin (OC) activities and expressions by mouse osteoblast-like MC3T3-E1 cells on HA were investigated at different experimental times in cultures, in comparison with those observed on titanium specimens used as a control (ET and ST). Cell proliferation was less in HA transformed cuttlefish bone scaffolds than in ET and ST specimens. In contrast, good performance for osteoblasts differentiation was observed on HA transformed cuttlefish bone scaffolds, similar to those observed onto titanium scaffolds.

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The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications

International Journal of Molecular Sciences ◽

10.3390/ijms22063086 ◽

2021 ◽

Vol 22 (6) ◽

pp. 3086

Author(s):

Stefanos Kikionis ◽

Efstathia Ioannou ◽

Eleni Aggelidou ◽

Leto-Aikaterini Tziveleka ◽

Efterpi Demiri ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Cell Attachment ◽

Hybrid Composites ◽

Sulfated Polysaccharide ◽

Biologically Active ◽

Bone Tissue Regeneration ◽

Natural Polymers ◽

The Impact

Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering. Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties. Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized. The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells. The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.

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A Review on Properties of Natural and Synthetic Based Electrospun Fibrous Materials for Bone Tissue Engineering

Membranes ◽

10.3390/membranes8030062 ◽

2018 ◽

Vol 8 (3) ◽

pp. 62 ◽

Cited By ~ 63

Author(s):

Deval Bhattarai ◽

Ludwig Aguilar ◽

Chan Park ◽

Cheol Kim

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biological Activities ◽

Biomechanical Properties ◽

Electrospun Fibers ◽

Fibrous Materials ◽

Interdisciplinary Field ◽

Versatile Technique ◽

Differentiation And Proliferation

Bone tissue engineering is an interdisciplinary field where the principles of engineering are applied on bone-related biochemical reactions. Scaffolds, cells, growth factors, and their interrelation in microenvironment are the major concerns in bone tissue engineering. Among many alternatives, electrospinning is a promising and versatile technique that is used to fabricate polymer fibrous scaffolds for bone tissue engineering applications. Copolymerization and polymer blending is a promising strategic way in purpose of getting synergistic and additive effect achieved from either polymer. In this review, we summarize the basic chemistry of bone, principle of electrospinning, and polymers that are used in bone tissue engineering. Particular attention will be given on biomechanical properties and biological activities of these electrospun fibers. This review will cover the fundamental basis of cell adhesion, differentiation, and proliferation of the electrospun fibers in bone tissue scaffolds. In the last section, we offer the current development and future perspectives on the use of electrospun mats in bone tissue engineering.

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Selective Laser Sintering of Polycaprolactone Bone Tissue Engineering Scaffolds

MRS Proceedings ◽

10.1557/proc-845-aa9.9 ◽

2004 ◽

Vol 845 ◽

Cited By ~ 2

Author(s):

Brock Partee ◽

Scott J. Hollister ◽

Suman Das

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Cell Attachment ◽

Selective Laser Sintering ◽

Laser Sintering ◽

Engineering Practice ◽

Micro Computed Tomography ◽

Tissue Engineering Scaffolds ◽

Differentiation And Proliferation

ABSTRACTPresent tissue engineering practice requires porous, bioresorbable scaffolds to serve as temporary 3D templates to guide cell attachment, differentiation, and proliferation. Recent research suggests that scaffold material and internal architecture significantly influence regenerate tissue structure and function. However, lack of versatile biomaterials processing methods have slowed progress towards fully testing these findings. Our research investigates using selective laser sintering (SLS) to fabricate bone tissue engineering scaffolds. Using SLS, we have fabricated polycaprolactone (PCL) and polycaprolactone/tri-calcium phosphate composite scaffolds. We report on scaffold design and fabrication, mechanical property measurements, and structural characterization via optical microscopy and micro-computed tomography.

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Dual Network Composites of Poly(vinyl alcohol)-Calcium Metaphosphate/Alginate with Osteogenic Ions for Bone Tissue Engineering in Oral and Maxillofacial Surgery

Bioengineering ◽

10.3390/bioengineering8080107 ◽

2021 ◽

Vol 8 (8) ◽

pp. 107

Author(s):

Lilis Iskandar ◽

Lucy DiSilvio ◽

Jonathan Acheson ◽

Sanjukta Deb

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Vinyl Alcohol ◽

Maxillofacial Surgery ◽

Bone Defects ◽

Bone Tissue Regeneration ◽

Poly Vinyl Alcohol ◽

Oral And Maxillofacial Surgery

Despite considerable advances in biomaterials-based bone tissue engineering technologies, autografts remain the gold standard for rehabilitating critical-sized bone defects in the oral and maxillofacial (OMF) region. A majority of advanced synthetic bone substitutes (SBS’s) have not transcended the pre-clinical stage due to inferior clinical performance and translational barriers, which include low scalability, high cost, regulatory restrictions, limited advanced facilities and human resources. The aim of this study is to develop clinically viable alternatives to address the challenges of bone tissue regeneration in the OMF region by developing ‘dual network composites’ (DNC’s) of calcium metaphosphate (CMP)—poly(vinyl alcohol) (PVA)/alginate with osteogenic ions: calcium, zinc and strontium. To fabricate DNC’s, single network composites of PVA/CMP with 10% (w/v) gelatine particles as porogen were developed using two freeze–thawing cycles and subsequently interpenetrated by guluronate-dominant sodium alginate and chelated with calcium, zinc or strontium ions. Physicochemical, compressive, water uptake, thermal, morphological and in vitro biological properties of DNC’s were characterised. The results demonstrated elastic 3D porous scaffolds resembling a ‘spongy bone’ with fluid absorbing capacity, easily sculptable to fit anatomically complex bone defects, biocompatible and osteoconductive in vitro, thus yielding potentially clinically viable for SBS alternatives in OMF surgery.

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Investigation of vitamin D-loaded silk fibroin electrospun scaffolds for bone tissue engineering applications

Materials Technology ◽

10.1080/10667857.2021.1940426 ◽

2021 ◽

pp. 1-9

Author(s):

Parisa Mostafavi ◽

Mitra Naeimi

Keyword(s):

Tissue Engineering ◽

Vitamin D ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Silk Fibroin ◽

Engineering Applications ◽

Electrospun Scaffolds

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A Bibliometric Analysis of the Global Trend of Using Alginate, Gelatine, and Hydroxyapatite for Bone Tissue Regeneration Applications

Polymers ◽

10.3390/polym13040647 ◽

2021 ◽

Vol 13 (4) ◽

pp. 647

Author(s):

Mohamed Saiful Firdaus Hussin ◽

Aludin Mohd Serah ◽

Khairul Azri Azlan ◽

Hasan Zuhudi Abdullah ◽

Maizlinda Izwana Idris ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bibliometric Analysis ◽

Research Work ◽

The United States ◽

Research Area ◽

Bone Tissue Regeneration ◽

Future Research ◽

Starting Point

Collecting information from previous investigations and expressing it in a scientometrics study can be a priceless guide to getting a complete overview of a specific research area. The aim of this study is to explore the interrelated connection between alginate, gelatine, and hydroxyapatite within the scope of bone tissue and scaffold. A review of traditional literature with data mining procedures using bibliometric analyses was considered to identify the evolution of the selected research area between 2009 and 2019. Bibliometric methods and knowledge visualization technologies were implemented to investigate diverse publications based on the following indicators: year of publication, document type, language, country, institution, author, journal, keyword, and number of citations. An analysis using a bibliometric study found that 7446 papers were located with the keywords “bone tissue” and “scaffold”, and 1767 (alginate), 185 (gelatine), 5658 (hydroxyapatite) papers with those specific sub keywords. The number of publications that relate to “tissue engineering” and bone more than doubled between 2009 (1352) and 2019 (2839). China, the United States and India are the most productive countries, while Sichuan University and the Chinese Academy of Science from China are the most important institutions related to bone tissue scaffold. Materials Science and Engineering C is the most productive journal, followed by the Journal of Biomedical Materials Research Part A. This paper is a starting point, providing the first bibliometric analysis study of bone tissue and scaffold considering alginate, gelatine and hydroxyapatite. A bibliometric analysis would greatly assist in giving a scientific insight to support desired future research work, not only associated with bone tissue engineering applications. It is expected that the analysis of alginate, gelatine and hydroxyapatite in terms of 3D bioprinting, clinical outcomes, scaffold architecture, and the regenerative medicine approach will enhance the research into bone tissue engineering in the near future. Continued studies into these research fields are highly recommended.

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Scaffolds for Growth Factor Delivery as Applied to Bone Tissue Engineering

International Journal of Polymer Science ◽

10.1155/2012/174942 ◽

2012 ◽

Vol 2012 ◽

pp. 1-25 ◽

Cited By ~ 43

Author(s):

Keith A. Blackwood ◽

Nathalie Bock ◽

Tim R. Dargaville ◽

Maria Ann Woodruff

Keyword(s):

Tissue Engineering ◽

Growth Factors ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Donor Site ◽

Structural Features ◽

Donor Site Morbidity ◽

Tissue Type ◽

Specific Cell ◽

Growth Factor Delivery

There remains a substantial shortfall in the treatment of severe skeletal injuries. The current gold standard of autologous bone grafting from the same patient has many undesirable side effects associated such as donor site morbidity. Tissue engineering seeks to offer a solution to this problem. The primary requirements for tissue-engineered scaffolds have already been well established, and many materials, such as polyesters, present themselves as potential candidates for bone defects; they have comparable structural features, but they often lack the required osteoconductivity to promote adequate bone regeneration. By combining these materials with biological growth factors, which promote the infiltration of cells into the scaffold as well as the differentiation into the specific cell and tissue type, it is possible to increase the formation of new bone. However due to the cost and potential complications associated with growth factors, controlling the rate of release is an important design consideration when developing new bone tissue engineering strategies. This paper will cover recent research in the area of encapsulation and release of growth factors within a variety of different polymeric scaffolds.

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