scholarly journals Evaluation of Conditions Affecting Properties of Gac (Momordica Cocochinensis Spreng) Oil-Loaded Solid Lipid Nanoparticles (SLNs) Synthesized Using High-Speed Homogenization Process

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 90 ◽  
Author(s):  
Huynh Mai ◽  
Thai Nguyen ◽  
Thi Le ◽  
Duy Nguyen ◽  
Long Bach
Keyword(s):  
Solid Lipid Nanoparticles ◽  
High Speed ◽  
Lipid A ◽  
Storage Temperature ◽  
Tween 80 ◽  
Storage Conditions ◽  
Lipid Nanoparticles ◽  
Homogenization Method ◽  
Solid Lipid ◽  
The Impact

In this study, we attempted the preparation of gac oil-loaded solid lipid nanoparticles (SLNs) by the high-speed homogenization method using Naterol SE solid lipid, a cosmetic self-emulsifying base, and surfactant and investigated the effects of different conditions on the characteristics of the resulting nanoparticles. The suspensions containing 5% active agents (gac-oil, w/w) were dispersed in a surfactant concentration of 5% (w/w) (Span 80:Tween 80 ratio of 28:72 w/w) and 2.5% (w/w) of solid lipid (Naterol SE) concentration. Suitable conditions for hot homogenization were 13,000 rpm, 60 min and 60 °C for speed, time and temperature, respectively. The suitable conditions for the subsequent cold homogenization were 25 min of homogenization time and 5 °C of temperature. The results showed that the mean size of SLNs-gac oil was 107 nm (measured by laser diffraction spectrometry, LDS), and dried size of SLNs-gac oil ranged from 50 to 80 nm (measured by transmission electron microscope, TEM). In addition, the study investigated the impact of gac oil content on the particle size of SLNs-gac oil and its stability under different storage conditions of UV radiation and storage temperature. At high storage temperatures, the color changes (ΔE) of the samples were more profound in comparison to that at the low storage temperature. The ΔE value of the blank sample (SLN-FREE gac-oil) was higher than that of the Gac oil-loaded SLNs samples (SLN-gac oil).

scholarly journals PREPARATION, CHARACTERIZATION, AND FORMULATION OF SOLID LIPID NANOPARTICLES LOTION FROM MULBERRY ROOTS (MORUS ALBA L.)

2020 ◽  
pp. 182-186
Author(s):  
MUHAMAD WILDAN NUGRAHA ◽  
RADITYA ISWANDANA ◽  
MAHDI JUFRI
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Solid Lipid Nanoparticles ◽  
High Speed ◽  
Tween 80 ◽  
Lipid Nanoparticles ◽  
Morus Alba ◽  
Glyceryl Monostearate ◽  
Optimum Result ◽  
Solid Lipid

Objective: Tween 80 has been used as a solvent for the extraction of phenolic compounds because this surfactant has both hydrophilic and hydrophobicproperties. Solid lipid nanoparticles (SLNs) have been developed to improve penetration through the skin layer. We investigated the efficacy of usingthe microwave-assisted micellar extraction (MAME) approach for extracting oxyresveratrol from Morus alba roots and also to develop an SLN lotion.Methods: The M. alba roots were extracted with Tween 80 in a microwave for 18 min, and the extract was used to develop SLN with differentconcentrations of glyceryl monostearate. The SLNs from M. alba root extracts were prepared by a high-speed homogenization technique (25,000 rpmfor 15 min). The SLNs produced were characterized as per particle size, polydispersity index (PDI), and zeta potential. The SLNs with the bestcharacteristics were used to formulate a lotion using a high-pressure homogenizer.Results: Extraction using MAME showed improved extraction efficiency. The oxyresveratrol concentration from the extract was 2.77%. The SLN with2.5% glyceryl monostearate showed the optimum result, with a particle size of 130.20 nm, a PDI of 0.278, and a zeta potential of −21.8 mV. The SLNlotion exhibited a particle size of 285.9 nm and a PDI of 0.360. The SLN lotion also had good penetration, with a flux of 4.70 μg cm−2/h.Conclusion: MAME is an efficient method for extracting oxyresveratrol from M. alba roots. The SLN with 2.5% glyceryl monostearate exhibited theoptimum characteristics, and the SLN lotion showed good characteristics, including skin penetration.

scholarly journals Formulation and Evaluation of Solid Lipid Nanoparticles of Olanzapine for the Treatment of Psychosis

2020 ◽  
Vol 10 (5-s) ◽  
pp. 25-31
Author(s):  
Shubhangi C. Daswadkar ◽  
Abhijit Vasant Atole
Keyword(s):  
Particle Size ◽  
Solid Lipid Nanoparticles ◽  
High Speed ◽  
Entrapment Efficiency ◽  
Antipsychotic Agent ◽  
Tween 80 ◽  
Lipid Nanoparticles ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Solid Lipid

Solid lipid nanoparticles (SLN) are typically spherical with an average diameter between 1 nm to 1000 nm in range. It is alternative carrier systems to tradition colloidal carriers, such as liposomes emulsions and polymeric micro and nanoparticles. Olanzapine (OZP) is an atypical antipsychotic agent which is used for treatment of Schizophrenia. Its oral bioavailability is around of 40%. OZP is a class II drug so it having low aqueous solubility. To overcome that problem and to increase its bioavailability, the solid lipid nanoparticles of olanzapine are prepared. Formulation batches designed by modifying type of surfactant ( Span 80, Tween 80), concentration of surfactant, Concentration of co-surfactant, type of lipid ( glyceryl monostearate, Stearic acid), Lipid concentration, speed of stirring and time of stirring using customised design  of DOE. The SLN were prepared by high speed homogenization technique, and then characterized by particle size analysis, Drug entrapment efficiency and Drug diffusion study. A formulation containing GMS as a lipid stabilised with tween 80 as surfactant show good drug release, smaller particle size, as compared with other formulations with different lipid and surfactant. The present research findings indicate that OZP loaded solid lipid Nano particulate system for delivery of OZP with better efficacy with minimum adverse effects. Keywords: Olanzapine, SLN, GMS, high speed homogenization and DOE.

Solid Lipid Nanoparticles from Active Compounds of Mango Seed Kernel Extract

2016 ◽  
Vol 675-676 ◽  
pp. 65-68
Author(s):  
Kornuma Kukaittinun ◽  
Chutimon Satirapipathkul
Keyword(s):  
Solid Lipid Nanoparticles ◽  
High Speed ◽  
Storage Temperature ◽  
Antioxidant Activities ◽  
Bioactive Compound ◽  
Lipid Nanoparticles ◽  
Seed Kernel ◽  
Solid Lipid ◽  
Mango Seed Kernel ◽  
Mango Seed

The bioactive compounds in Mango seed kernel have high antioxidant activities which can be used as an ingredient in cosmetic products. The aim of this study was to improve the stability of bioactive compound in seed kernel extract by preparation in the form of solid lipid nanoparticles (SLN). Solid lipid nanoparticles (SLN) were prepared by high-speed homogenization technique. The effect of formulation on introduction the extract in to the base solid lipid nanoparticle system was investigated. At the optimal condition, the particle size was 329.1 nm. The highest load efficiency was 89.12%. The physical stability under the different storage temperature for 15 days indicated that the mango kernel extract entrapped in nanoparticles showed good stability. No obvious changes of clarity and degradation were found.

Optimization of Solid Lipid Nanoparticles of Ezetimibe in Combination with Simvastatin Using Quality by Design (QbD)

2020 ◽  
Vol 10 (4) ◽  
pp. 404-418
Author(s):  
Kruti Borderwala ◽  
Ganesh Swain ◽  
Namrata Mange ◽  
Jaimini Gandhi ◽  
Manisha Lalan ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid Lipid Nanoparticles ◽  
High Speed ◽  
Entrapment Efficiency ◽  
Lipid Nanoparticles ◽  
Water Soluble ◽  
Lipid Matrix ◽  
Solid Lipid ◽  
32 Factorial Design ◽  
Full Factorial Experimental Design

Background: The objective of this study was to develop solid lipid nanoparticles (SLNs) of poorly water soluble anti-hyperlipidemic drugs-Ezetimibe in combination with Simvastatin. Methods: This study describes a 32 full factorial experimental design to optimize the formulation of drug loaded lipid nanoparticles (SLN) by the high speed homogenization technique. The independent variables amount of lipid (GMS) and amount of surfactant (Poloxamer 188) were studied at three levels and arranged in a 32 factorial design to study the influence on the response variables- particle size, % entrapment efficiency (%EE) and cumulative drug release (% CDR) at 24 h. Results: The particle size, % EE and % CDR at 24 h for the 9 batches (B1 to B9) showed a wide variation of 104.6-496.6 nm, 47.80-82.05% (Simvastatin); 48.60-84.23% (Ezetimibe) and 54.64-92.27% (Simvastatin); 43.8-97.1% (Ezetimibe), respectively. The responses of the design were analysed using Design Expert 10.0.2. (Stat-Ease, Inc, USA), and the analytical tools of software were used to draw response surface plots. From the statistical analysis of data, polynomial equations were generated. Optimized formulation showed particle size of 169.5 nm, % EE of 75.43% (Simvastatin); 79.10% (Ezetimibe) and 74.13% (Simvastatin); 77.11% (Ezetimibe) %CDR after 24 h. Thermal analysis of prepared solid lipid nanoparticles gave indication of solubilisation of drugs within lipid matrix. Conclusion: Fourier Transformation Infrared Spectroscopy (FTIR) showed the absence of new bands for loaded solid lipid nanoparticles indicating no interaction between drugs and lipid matrix and being only dissolved in it. Electron microscope of transmission techniques indicated sphere form of prepared solid lipid nanoparticles with smooth surface with size approximately around 100 nm.

Effect of Surfactant on Characteristics of Solid Lipid Nanoparticles (SLN)

2011 ◽  
Vol 364 ◽  
pp. 313-316 ◽  
Author(s):  
Karn Orachai Kullavadee ◽  
Ruktanonchai Uracha ◽  
Siwaporn Meejoo Smith
Keyword(s):  
Particle Size ◽  
Solid Lipid Nanoparticles ◽  
Drug Carrier ◽  
Tween 80 ◽  
Lipid Nanoparticles ◽  
Great Promise ◽  
Solid Lipid ◽  
Alternative Drug ◽  
Model Drug ◽  
Drug Free

SLN have shown a great promise as an alternative drug carrier for intravenous and dermal applications. This work focuses on the basic properties of drug-free Compritol® ATO 888 based SLN systems by using cationic surfactant (CPC) and nonionic surfactant (Tween 80). Effects of surfactant on the physical properties of SLNs were investigated in the absence of model drug to avoid the interaction between drug and surfactant. These SLN samples have different particle size, zeta potential and morphology. DSC was used to quantify the crystallinity of SLN systems. It was found that %RI of both SLNs was similar, indicating that types of surfactant did not affect on crystallization of solid lipid. Spherical-like particle was observed with SLN-C, while rod-like particle was found with SLN-T. The results demonstrated that surfactant plays an important role on SLN physical characteristics.

scholarly journals Formulation, Optimization and Pharmacodynamic Studies of Pioglitazone HCl Solid Lipid Nanoparticles

2021 ◽  
pp. 329-341
Author(s):  
Y. Indira Muzib ◽  
E. Ramya ◽  
Y. R. Ambedkar
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Reference Group ◽  
Test Group ◽  
Tween 80 ◽  
Lipid Nanoparticles ◽  
Control Group ◽  
Albino Rats ◽  
Placebo Control ◽  
Solid Lipid ◽  
Group I

Pioglitazone HCl is an oral anti-diabetic agent used for the treatment of diabetes mellitus type II. The aim of the present work is to evaluate the pharmacodynamic activity of solid lipid nanoparticles of pioglitazone HCL prepared by using solvent injection technique and to compare with the control and test group. Among all the formulations, F5 was found to possess highest in-vitro drug release within 24 hrs i.e., 95.02±1.26%. The in vivo studies were performed using male albino rats of wistar strain (150-200g). Rats were divided in to five groups (n=6), group-I normal, group-II diabetes control, group-III   placebo control, group-IV reference, group-V test group. Diabetes was induced by streptazocin (60 mg/kg) by intraperitonial route. The reference group was treated with marketed tablet of pioglitazone HCL, test groups were treated with SLNs suspended in 0.1% Tween 80 and given to animals through oral gavages. Blood samples were collected by retro-orbital puncture before treatment, and after treatment at time intervals 0, 2, 4, 6, 8, 10, 12 and 24h in anti-coagulated vials. Parameters like glucose, tri glycerides (TG), total cholesterol (TC) and HDL-C were estimated by calorimetric method.  Diabetes induced rats showed elevated levels of glucose, TG, TC and reduced HDL. The oral administration of drug loaded SLNs in 0.1% Tween 80 solution showed reduced levels of glucose, TG and elevated levels of HDL-C and slightly reduced levels of TG in 24 h where as the marketed tablet showed reduced levels of glucose, TG and TC up-to 12 h and in 24thh  the glucose levels get elevated. Thus the optimized SLNs showed prolonged activity.

scholarly journals Long-term Stable Cationic Solid Lipid Nanoparticles for the Enhanced Intracellular Delivery of SMAD3 Antisense Oligonucleotides in Activated Murine Macrophages

2012 ◽  
Vol 15 (3) ◽  
pp. 467 ◽  
Author(s):  
Su-Eon Jin ◽  
Chong-Kook Kim
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Antisense Oligonucleotides ◽  
Lipid Nanoparticles ◽  
Homogenization Method ◽  
Tween 20 ◽  
Solid Lipid ◽  
Intracellular Activity ◽  
Macrophage Cells ◽  
Anti Inflammatory

Purpose: Long-term stable cationic solid lipid nanoparticles (cSLNs) were formulated to transfer SMAD3 antisense oligonucleotides (ASOs) into the cells to enhance the intracellular activity of the ASOs. The SMAD3 ASOs were designed to block the inflammatory processes linked to TGFβ/SMAD3 pathway. Methods: The cSLN formulation was prepared by high-pressure homogenization method composed of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), dioleoylphosphoethanolamine (DOPE), Tween 20, and tricaprin as a solid lipid core (1:1:1:1.67, w/w). The size and the zeta potential of the prepared cSLNs were measured by light scattering. The cSLN/ASO complexes were generated and introduced into the murine macrophage cells. After the treatment of the complexes, the cellular uptake of the complexes was determined by flow cytometry and the intracellular activity of SMAD3 ASOs from the complexes was evaluated by western blotting of SMAD3. In addition, TGFβ1, an upstream molecule of TGFβ/SMAD3 pathway, was monitored by ELISA. Results: The nano-scale sized cSLNs were positively charged and physically stable at 4oC during the storage up to 24 months. The uptake efficiency of the cSLN/ASO complexes into macrophage cells was enhanced up to 80% without cytotoxicity. After the treatment of the cSLN/ASO complexes, SMAD3 as well as TGFβ1 was significantly suppressed based on the SMAD3 ASO activity in the macrophage cells. In addition, the cSLN/ASO complexes prevented the morphological change to dendritic shape in the activated macrophage cells. Conclusion: These results suggest that the cSLNs have a potential to deliver the SMAD3 ASOs to intracellular compartments for the anti-inflammatory effect. The development of this strategy might lead to anti-inflammatory and anti-fibrotic therapies in immunological disorders. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Sign in / Sign up

Export Citation Format

Share Document