Solid Lipid Nanoparticles from Active Compounds of Mango Seed Kernel Extract

The bioactive compounds in Mango seed kernel have high antioxidant activities which can be used as an ingredient in cosmetic products. The aim of this study was to improve the stability of bioactive compound in seed kernel extract by preparation in the form of solid lipid nanoparticles (SLN). Solid lipid nanoparticles (SLN) were prepared by high-speed homogenization technique. The effect of formulation on introduction the extract in to the base solid lipid nanoparticle system was investigated. At the optimal condition, the particle size was 329.1 nm. The highest load efficiency was 89.12%. The physical stability under the different storage temperature for 15 days indicated that the mango kernel extract entrapped in nanoparticles showed good stability. No obvious changes of clarity and degradation were found.

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Evaluation of Conditions Affecting Properties of Gac (Momordica Cocochinensis Spreng) Oil-Loaded Solid Lipid Nanoparticles (SLNs) Synthesized Using High-Speed Homogenization Process

Processes ◽

10.3390/pr7020090 ◽

2019 ◽

Vol 7 (2) ◽

pp. 90 ◽

Cited By ~ 17

Author(s):

Huynh Mai ◽

Thai Nguyen ◽

Thi Le ◽

Duy Nguyen ◽

Long Bach

Keyword(s):

Solid Lipid Nanoparticles ◽

High Speed ◽

Lipid A ◽

Storage Temperature ◽

Tween 80 ◽

Storage Conditions ◽

Lipid Nanoparticles ◽

Homogenization Method ◽

Solid Lipid ◽

The Impact

In this study, we attempted the preparation of gac oil-loaded solid lipid nanoparticles (SLNs) by the high-speed homogenization method using Naterol SE solid lipid, a cosmetic self-emulsifying base, and surfactant and investigated the effects of different conditions on the characteristics of the resulting nanoparticles. The suspensions containing 5% active agents (gac-oil, w/w) were dispersed in a surfactant concentration of 5% (w/w) (Span 80:Tween 80 ratio of 28:72 w/w) and 2.5% (w/w) of solid lipid (Naterol SE) concentration. Suitable conditions for hot homogenization were 13,000 rpm, 60 min and 60 °C for speed, time and temperature, respectively. The suitable conditions for the subsequent cold homogenization were 25 min of homogenization time and 5 °C of temperature. The results showed that the mean size of SLNs-gac oil was 107 nm (measured by laser diffraction spectrometry, LDS), and dried size of SLNs-gac oil ranged from 50 to 80 nm (measured by transmission electron microscope, TEM). In addition, the study investigated the impact of gac oil content on the particle size of SLNs-gac oil and its stability under different storage conditions of UV radiation and storage temperature. At high storage temperatures, the color changes (ΔE) of the samples were more profound in comparison to that at the low storage temperature. The ΔE value of the blank sample (SLN-FREE gac-oil) was higher than that of the Gac oil-loaded SLNs samples (SLN-gac oil).

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Optimization of Solid Lipid Nanoparticles of Ezetimibe in Combination with Simvastatin Using Quality by Design (QbD)

Nanoscience &Nanotechnology-Asia ◽

10.2174/2210681209666190218143736 ◽

2020 ◽

Vol 10 (4) ◽

pp. 404-418

Author(s):

Kruti Borderwala ◽

Ganesh Swain ◽

Namrata Mange ◽

Jaimini Gandhi ◽

Manisha Lalan ◽

...

Keyword(s):

Particle Size ◽

Solid Lipid Nanoparticles ◽

High Speed ◽

Entrapment Efficiency ◽

Lipid Nanoparticles ◽

Water Soluble ◽

Lipid Matrix ◽

Solid Lipid ◽

32 Factorial Design ◽

Full Factorial Experimental Design

Background: The objective of this study was to develop solid lipid nanoparticles (SLNs) of poorly water soluble anti-hyperlipidemic drugs-Ezetimibe in combination with Simvastatin. Methods: This study describes a 32 full factorial experimental design to optimize the formulation of drug loaded lipid nanoparticles (SLN) by the high speed homogenization technique. The independent variables amount of lipid (GMS) and amount of surfactant (Poloxamer 188) were studied at three levels and arranged in a 32 factorial design to study the influence on the response variables- particle size, % entrapment efficiency (%EE) and cumulative drug release (% CDR) at 24 h. Results: The particle size, % EE and % CDR at 24 h for the 9 batches (B1 to B9) showed a wide variation of 104.6-496.6 nm, 47.80-82.05% (Simvastatin); 48.60-84.23% (Ezetimibe) and 54.64-92.27% (Simvastatin); 43.8-97.1% (Ezetimibe), respectively. The responses of the design were analysed using Design Expert 10.0.2. (Stat-Ease, Inc, USA), and the analytical tools of software were used to draw response surface plots. From the statistical analysis of data, polynomial equations were generated. Optimized formulation showed particle size of 169.5 nm, % EE of 75.43% (Simvastatin); 79.10% (Ezetimibe) and 74.13% (Simvastatin); 77.11% (Ezetimibe) %CDR after 24 h. Thermal analysis of prepared solid lipid nanoparticles gave indication of solubilisation of drugs within lipid matrix. Conclusion: Fourier Transformation Infrared Spectroscopy (FTIR) showed the absence of new bands for loaded solid lipid nanoparticles indicating no interaction between drugs and lipid matrix and being only dissolved in it. Electron microscope of transmission techniques indicated sphere form of prepared solid lipid nanoparticles with smooth surface with size approximately around 100 nm.

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PREPARATION, CHARACTERIZATION, AND FORMULATION OF SOLID LIPID NANOPARTICLES LOTION FROM MULBERRY ROOTS (MORUS ALBA L.)

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s1.ff041 ◽

2020 ◽

pp. 182-186

Author(s):

MUHAMAD WILDAN NUGRAHA ◽

RADITYA ISWANDANA ◽

MAHDI JUFRI

Keyword(s):

Particle Size ◽

Zeta Potential ◽

Solid Lipid Nanoparticles ◽

High Speed ◽

Tween 80 ◽

Lipid Nanoparticles ◽

Morus Alba ◽

Glyceryl Monostearate ◽

Optimum Result ◽

Solid Lipid

Objective: Tween 80 has been used as a solvent for the extraction of phenolic compounds because this surfactant has both hydrophilic and hydrophobicproperties. Solid lipid nanoparticles (SLNs) have been developed to improve penetration through the skin layer. We investigated the efficacy of usingthe microwave-assisted micellar extraction (MAME) approach for extracting oxyresveratrol from Morus alba roots and also to develop an SLN lotion.Methods: The M. alba roots were extracted with Tween 80 in a microwave for 18 min, and the extract was used to develop SLN with differentconcentrations of glyceryl monostearate. The SLNs from M. alba root extracts were prepared by a high-speed homogenization technique (25,000 rpmfor 15 min). The SLNs produced were characterized as per particle size, polydispersity index (PDI), and zeta potential. The SLNs with the bestcharacteristics were used to formulate a lotion using a high-pressure homogenizer.Results: Extraction using MAME showed improved extraction efficiency. The oxyresveratrol concentration from the extract was 2.77%. The SLN with2.5% glyceryl monostearate showed the optimum result, with a particle size of 130.20 nm, a PDI of 0.278, and a zeta potential of −21.8 mV. The SLNlotion exhibited a particle size of 285.9 nm and a PDI of 0.360. The SLN lotion also had good penetration, with a flux of 4.70 μg cm−2/h.Conclusion: MAME is an efficient method for extracting oxyresveratrol from M. alba roots. The SLN with 2.5% glyceryl monostearate exhibited theoptimum characteristics, and the SLN lotion showed good characteristics, including skin penetration.

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Curcumin Containing PEGylated Solid Lipid Nanoparticles for Systemic Administration: A Preliminary Study

Molecules ◽

10.3390/molecules25132991 ◽

2020 ◽

Vol 25 (13) ◽

pp. 2991 ◽

Cited By ~ 2

Author(s):

Debora Santonocito ◽

Maria Grazia Sarpietro ◽

Claudia Carbone ◽

Annamaria Panico ◽

Agata Campisi ◽

...

Keyword(s):

Zeta Potential ◽

Solid Lipid Nanoparticles ◽

Antioxidant Activities ◽

Lipid Nanoparticles ◽

Systemic Administration ◽

Term Stability ◽

Long Term Stability ◽

Solid Lipid ◽

Wide Range

Curcumin (CUR) has a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities, and it can be considered a good candidate for the potential treatment of central nervous system (CNS) pathologies, although its use in clinical practice is compromised due to its high lipophilicity. Solid lipid nanoparticles (SLNs) are well-known nanocarriers representing a consolidated approach for the delivery of lipophilic compounds, but their systemic use is limited due their short half-life. The formulation of stealth SLNs (pSLNs) could be a valid strategy to overcome this limit. Curcumin-loaded-pSLNs were prepared by the solvent evaporation method. Formulation was characterized for their mean size, zeta potential, size distribution, and morphology. Drug antioxidant activity was evaluated by Oxygen Radical Absorbance Capacity (ORAC) assay. Finally, the obtained formulations were analyzed in terms of long-term stability. Curcumin-loaded-pSLNs showed good technological parameters with a mean particle size below 200 nm, as confirmed by TEM images, and a zeta potential value around −30 mV, predicting good long-term stability. Differential Scanning Calorimetry (DSC) analysis confirmed that PEG micelles interacted with the SLN surface; this suggests the location of the PEG on the pSLN surface. Therefore, these preliminary studies suggest that the produced formulation could be regarded as a promising carrier for the systemic administration.

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Solid Lipid Nanoparticles: A Promising Nanomaterial in Drug Delivery

Current Pharmaceutical Design ◽

10.2174/1381612825666190903155321 ◽

2019 ◽

Vol 25 (37) ◽

pp. 3943-3959 ◽

Cited By ~ 3

Author(s):

Kuldeep Rajpoot

Keyword(s):

Solid Lipid Nanoparticles ◽

High Speed ◽

Double Emulsion ◽

Lipid Nanoparticles ◽

Suitable Candidate ◽

Pharmaceutical Ingredients ◽

Solid Lipid ◽

Drug Molecules ◽

Efficient Delivery ◽

Specific Ligand

The solid lipid nanoparticles (SLNs) usually consists of active drug molecules along with solid lipids, surfactants, and/or co-surfactants. They possess some potential features such as nano-size, surface with a free functional group to attach ligands, and as well they prove safe homing for both lipophilic as well as hydrophilic molecules. As far as synthesis is concerned, SLNs can be prepared by employing various techniques viz., homogenization techniques (e.g., high-pressure, high-speed, cold, or hot homogenization), spray drying technique, ultrasonication, solvent emulsification, double emulsion technique, etc. Apart from this, they are characterized by different methods for determining various parameters like particle-size, polydispersity-index, surface morphology, DSC, XRD, etc. SLNs show good stability as well as the ability for surface tailoring with the specific ligand, which makes them a suitable candidate in the therapy of numerous illnesses, especially in the targeting of the cancers. In spite of this, SLNs have witnessed their application via various routes e.g., oral, parenteral, topical, pulmonary, rectal routes, etc. Eventually, SLNs have also shown great potential for delivery of gene/DNA, vaccines, as well as in cosmeceuticals. Hence, SLNs have emerged as a promising nanomaterial for efficient delivery of various Active Pharmaceutical Ingredients (APIs).

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Selection of Cryoprotectant in Lyophilization of Progesterone-Loaded Stearic Acid Solid Lipid Nanoparticles

Pharmaceutics ◽

10.3390/pharmaceutics12090892 ◽

2020 ◽

Vol 12 (9) ◽

pp. 892 ◽

Cited By ~ 2

Author(s):

Timothy M. Amis ◽

Jwala Renukuntla ◽

Pradeep Kumar Bolla ◽

Bradley A. Clark

Keyword(s):

Particle Size ◽

Stearic Acid ◽

Solid Lipid Nanoparticles ◽

High Speed ◽

Sodium Taurocholate ◽

Polarized Light ◽

Lipid Nanoparticles ◽

Stability Study ◽

Solid Lipid ◽

Selection Of

Cryoprotectants are often required in lyophilization to reduce or eliminate agglomeration of solute or suspended materials. The aim of this study was to select a cryoprotecting agent and optimize its concentration in a solid lipid nanoparticle formulation. Progesterone-loaded stearic acid solid lipid nanoparticles (SA-P SLNs) were prepared by hot homogenization with high speed mixing and sonication. The stearic acid content was 4.6% w/w and progesterone was 0.46% w/w of the initial formulation. Multiple surfactants were evaluated, and a lecithin and sodium taurocholate system was chosen. Three concentrations of surfactant were then evaluated, and a concentration of 2% w/w was chosen based on particle size, polydispersity, and zeta potential. Agglomeration of SA-P SLNs after lyophilization was observed as measured by increased particle size. Dextran, glycine, mannitol, polyvinylpyrrolidone (PVP), sorbitol, and trehalose were evaluated as cryoprotectants by both an initial freeze–thaw analysis and after lyophilization. Once selected as the cryoprotectant, trehalose was evaluated at 5%, 10%, 15%, and 20% for optimal concentration, with 20% trehalose being finally selected as the level of choice. Evaluation by DSC confirmed intimate interaction between stearic acid and progesterone in the SA-P SLNs, and polarized light microscopy shows successful lyophilization of the trehalose/SA-P SLN. A short term 28-day stability study suggests the need for refrigeration of the final lyophilized SA-P SLNs in moisture vapor impermeable packaging.

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REVIEW ARTIKEL: SOLID LIPID NANOPARTICLES (SLN) METODE DAN KARAKTERISTIK

Jurnal Insan Farmasi Indonesia ◽

10.36387/jifi.v3i2.548 ◽

2020 ◽

Vol 3 (2) ◽

pp. 307-316

Author(s):

Ilhanni Khoerunisa ◽

◽

Aji Najihudin ◽

Siti Hindun

Keyword(s):

Particle Size ◽

Small Particle ◽

Solid Lipid Nanoparticles ◽

High Speed ◽

Entrapment Efficiency ◽

Lipid Nanoparticles ◽

Absorption Efficiency ◽

Small Particle Size ◽

Solid Fat ◽

Solid Lipid

Solid Lipid Nanoparticles are composed of active substances, solid fats, water and surfactants. SLN has advantages by having a small particle size of 50-1000 nm and a large absorption efficiency value of 40-100%. SLN has a homogeneous particle size with a IP value that is closed to zero. One of the methods used to produce SLN is HSH/ High pressure homogeneity where the process uses pressure to produce small particle size. The HSH/ High-speed homogeneity method uses stirring speed as a parameter in the manufacture of nanoparticles. Solvent emulsification method does not use high temperatures in the homogeneity process. The most important SLN characteristics are particle size and entrapment efficiency values. These characteristics are strongly influenced by large concentration of solid fat and surfactants. The greater amount of the surfactant used, the smaller the particle size and the increasing value of the trap efficiency. If the solid fat concentration used is greater, the particle size will be as larger, but the absorption efficiency value will be greater because the fat will provide more space for the active substance to be entrapped.

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Formulation and Evaluation of Solid Lipid Nanoparticles of Olanzapine for the Treatment of Psychosis

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i5-s.4440 ◽

2020 ◽

Vol 10 (5-s) ◽

pp. 25-31

Author(s):

Shubhangi C. Daswadkar ◽

Abhijit Vasant Atole

Keyword(s):

Particle Size ◽

Solid Lipid Nanoparticles ◽

High Speed ◽

Entrapment Efficiency ◽

Antipsychotic Agent ◽

Tween 80 ◽

Lipid Nanoparticles ◽

Particle Size Analysis ◽

Size Analysis ◽

Solid Lipid

Solid lipid nanoparticles (SLN) are typically spherical with an average diameter between 1 nm to 1000 nm in range. It is alternative carrier systems to tradition colloidal carriers, such as liposomes emulsions and polymeric micro and nanoparticles. Olanzapine (OZP) is an atypical antipsychotic agent which is used for treatment of Schizophrenia. Its oral bioavailability is around of 40%. OZP is a class II drug so it having low aqueous solubility. To overcome that problem and to increase its bioavailability, the solid lipid nanoparticles of olanzapine are prepared. Formulation batches designed by modifying type of surfactant ( Span 80, Tween 80), concentration of surfactant, Concentration of co-surfactant, type of lipid ( glyceryl monostearate, Stearic acid), Lipid concentration, speed of stirring and time of stirring using customised design of DOE. The SLN were prepared by high speed homogenization technique, and then characterized by particle size analysis, Drug entrapment efficiency and Drug diffusion study. A formulation containing GMS as a lipid stabilised with tween 80 as surfactant show good drug release, smaller particle size, as compared with other formulations with different lipid and surfactant. The present research findings indicate that OZP loaded solid lipid Nano particulate system for delivery of OZP with better efficacy with minimum adverse effects. Keywords: Olanzapine, SLN, GMS, high speed homogenization and DOE.

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