scholarly journals Screening and Diversity Analysis of Aerobic Denitrifying Phosphate Accumulating Bacteria Cultivated from A2O Activated Sludge

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 827 ◽  
Author(s):  
Yong Li ◽  
Siyuan Zhao ◽  
Jiejie Zhang ◽  
Yang He ◽  
Jianqiang Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Removal Rate ◽  
Sewage Treatment ◽  
Phosphorus Uptake ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Molecular Technique ◽  
Rich Diversity

The aerobic denitrifying phosphate accumulating bacteria (ADPB) use NO3− as an electron acceptor and remove nitrate by denitrification and concomitant uptake of excessive phosphorus in aerobic conditions. Activated sludge was collected from the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town, Chengdu City. The candidate ADPB strains were obtained by cultivation in the enriched denitrification media, followed by repeated isolation and purification on bromothymol blue (BTB) solid plates. The obtained candidates were further screened for ADPB strains by phosphorus uptake experiment, nitrate reduction test, metachromatic granules staining, and poly-β-hydroxybutyrate (PHB) staining. The 16 sedimentation ribosome deoxyribonucleic acid (16 S rDNA) molecular technique was used to determine their taxonomy. Further, the denitrification and dephosphorization capacities of ADPB strains were ascertained through their growth characteristics in nitrogen-phosphorus-rich liquid media. The results revealed a total of 25 ADPB strains screened from the activated sludge of the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town. These strains belonged to two classes, four orders, and five genera. Among them, the strain SW18NP2 was a potentially new species in the Acinetobacter genus, while the strain SW18NP24 was a potential new species in the Pseudomonas genus at the time of their characterization. The Acinetobacter was the dominant genus. The obtained ADPB strains demonstrated a rich diversity. The ADPB strains had significant variations in denitrification and dephosphorization capacities. Twenty-three strains exhibited a total phosphorus removal rate of above 50%, and 19 strains exhibited a total nitrogen removal rate of above 50%. The strain SW18NP2 showed the best denitrifying phosphorus removal (DPR) capacity, with a dephosphorization rate of 82.32% and a denitrification rate of 73.73%. The ADPB in the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town demonstrated a rich diversity and a strong DPR capacity.

scholarly journals The Microbiota and Abundance of the Class 1 Integron-Integrase Gene in Tropical Sewage Treatment Plant Influent and Activated Sludge

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0131532 ◽  
Author(s):  
Magna C. Paiva ◽  
Marcelo P. Ávila ◽  
Mariana P. Reis ◽  
Patrícia S. Costa ◽  
Regina M. D. Nardi ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Class 1 Integron ◽  
Integrase Gene ◽  
Class 1

Start-Up of A2/O Humic Activated Sludge Systemphosphorus Removal Efficiency Analysis

2010 ◽  
Vol 113-116 ◽  
pp. 2201-2207 ◽  
Author(s):  
Jun Yin ◽  
Lei Wu ◽  
Ke Zhao ◽  
Yu Juan Yu
Keyword(s):  
Activated Sludge ◽  
Removal Efficiency ◽  
Phosphorus Removal ◽  
Removal Rate ◽  
Phosphorus Uptake ◽  
Phosphorus Release ◽  
Activated Sludge System ◽  
Start Up ◽  
Article Analysis ◽  
Sludge Activity

In this article, analysis the start-up of A2/O humic activated sludge system phosphorus removal efficiency and the characteristics of anaerobic phosphorus release, aerobic phosphorus uptake, sludge activity and their change in the Series Technologies process. The results show that A2/O humic activated sludge system phosphorus removal rate stabilized at 90.7% ~ 97.6%. Sludge activity except for anoxic zone 2 increased, along the process showed a gradual decrease trend.

Impact of cold and dilute sewage on pre-fermentation - a case study

2001 ◽  
Vol 43 (11) ◽  
pp. 109-117 ◽  
Author(s):  
D. Bixio ◽  
P. van Hauwermeiren ◽  
C. Thoeye ◽  
P. Ockier
Keyword(s):  
Phosphorus Removal ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
High Rate ◽  
Cold Weather ◽  
Biological Nutrient Removal ◽  
Municipal Sewage ◽  
Biological Phosphorus Removal ◽  
Primary Sludge

The municipal sewage treatment plant (STP) of the city of Ghent (Belgium) has to be retrofitted to a 43%-increase in the nitrogen treatment capacity and to phosphorus removal. Cold weather, dilute sewage and a critical COD over N ratio make the retrofit a challenge for full biological nutrient removal. The potential for fermentation of primary sludge to alter those critical feed sewage characteristics was experimentally evaluated. The idea was that the pinpoint introduction of fermentate could optimise the available reactors by achieving high-rate denitrification and enhanced biological phosphorus removal. The fermentation process was evaluated with a bench scale apparatus. At 20°C (heated process), the hydrolysis yield - expressed in terms of soluble COD - varied from 11% to 24% of the total sludge COD. The fermentation yield expressed in VFA COD varied from 8% to 13% of the total sludge COD. The efficiency of heated fermentation of primary sludge was lower during cold and wet weather, due to the different sewage characteristics, as a result of extended dilution periods and low temperature. The raw sewage, the primary effluent and the fermentate were fractionated according to the requirements for the IAWQ Activated Sludge Model No. 2d. The results clearly show that fermentation in the sewer played an important role and temperature was the driving parameter for the characteristics of the dissolved COD. Instead, the weather flow conditions were the driving parameter for the characteristics of the suspended COD. The results of the detailed fractionation were used as background for process evaluation. The final scenario choice for the retrofit depends on a cost-efficiency calculation.

Thermochemical pretreatment in the anaerobic digestion of waste activated sludge

2002 ◽  
Vol 46 (10) ◽  
pp. 173-179 ◽  
Author(s):  
S. Tanaka ◽  
K. Kamiyama
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Municipal Sewage ◽  
Waste Activated Sludge ◽  
Anaerobic Digesters ◽  
Thermochemical Pretreatment ◽  
Methane Production Rate

Effects of a thermochemical pretreatment on the anaerobic digestion of waste activated sludge (WAS) was investigated by semicontinuously-fed digesters operated at 37¡C. WAS from a return sludge line of a municipal sewage treatment plant was pretreated by autoclaving at 130°C for 5 minutes after adding 0.3g NaOH/g VSS. Solids of WAS were thermochemically solubilized to one half and then 60% or more were in totality solubilized in anaerobic digesters fed with pretreated WAS at 2-8 days of hydraulic retention times (HRT), while only 16-36% were solubilized in digesters fed with raw WAS. The adverse effect of the set temperature (130°C) on the biodegradability of protein was not found. As a result, removal rates of COD in digestion was increased from 38% to 57% at 8 days HRT by the pretreatment. A specific methane production rate in the pretreated process was three times as high as the normal process. The thermochemical pretreatment was found to be very effective to enhance biodegradability as well as solubilization of WAS in anaerobic digestion.

scholarly journals Occurrence of Giardia cysts and Cryptosporidium oocysts in activated sludge samples in Campinas, SP, Brazil

2004 ◽  
Vol 46 (6) ◽  
pp. 309-313 ◽  
Author(s):  
Luciana Urbano Santos ◽  
Taís Rondello Bonatti ◽  
Romeu Cantusio Neto ◽  
Regina Maura Bueno Franco
Keyword(s):  
Activated Sludge ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
High Concentration ◽  
Potential Source ◽  
Two Samples ◽  
Cryptosporidium Oocysts ◽  
Flotation Method ◽  
Giardia Cysts

Giardia and Cryptosporidium have caused several outbreaks of gastroenteritis in humans associated with drinking water. Contaminated sewage effluents are recognized as a potential source of waterborne protozoa. Due to the lack of studies about the occurrence of these parasites in sewage samples in Brazil, we compared the efficiency of two procedures for concentrating cysts and oocysts in activated sludge samples of one sewage treatment plant. For this, the samples were submitted to i) concentration by the ether clarification procedure (ECP) and to ii) purification by sucrose flotation method (SFM) and aliquots of the pellets were examined by immunofluorescence. Giardia cysts were present in all samples (100.0%; n = 8) when using ECP and kit 1 reagents, while kit 2 resulted in six positive samples (85.7%; n = 7). As for SFM, cysts were detected in 75.0% and 100.0% of these samples (for kit 1 and 2, respectively). Regarding Cryptosporidium, two samples (25.0%; kit 1 and 28.5% for kit 2) were detected positive by using ECP, while for SFM, only one sample (examined by kit 1) was positive (12.5%). The results of the control trial revealed Giardia and Cryptosporidium recovery efficiency rates for ECP of 54.5% and 9.6%, while SFM was 10.5% and 3.2%, respectively. Considering the high concentration detected, a previous evaluation of the activated sludge before its application in agriculture is recommended and with some improvement, ECP would be an appropriate simple technique for protozoa detection in sewage samples.

The Infuence of the Depth of the Secondary Sedimentation Tanks on the Sedimentation Efficiency at Bromma Sewage Treatment Plant

1988 ◽  
Vol 20 (4-5) ◽  
pp. 143-152 ◽  
Author(s):  
M. Tendaj-Xavier ◽  
J. Hultgren
Keyword(s):  
Activated Sludge ◽  
Suspended Solids ◽  
Ferrous Sulphate ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Design Flow ◽  
Activated Sludge Process ◽  
Solids Concentration ◽  
Rapid Flow

Bromma sewage treatment plant is the second largest plant in Stockholm with a design flow of 160,000 m3/d. The wastewater is treated mechanically, chemically by pre-precipitation with ferrous sulphate, and biologically by the activated sludge process. The requirements for the plant are 8 mg BOD7/l, 0.4 mg P/l and 2 mg NH4+-N/l. The requirement for ammonia refers to the period July-October. In order to meet those rather stringent requirements, the biological step was expanded 3 years ago with 6 new sedimentation tanks. The 6 new tanks have the same area as the 6 old ones but they have only a depth of 3.7 m compared with the depth of the old tanks, 5.7 m. Experience from the first years of operation of the new tanks is that these tanks are more sensitive and less efficient than the older ones. It seems that the effluent suspended solids concentration from the old tanks is less influenced by rapid flow variations than the concentration in the effluent from the new secondary sedimentation tanks. During the nitrification period denitrification takes place to some degree in the secondary sedimentation tanks. This may cause loss of solids and it has been observed that the deeper old tanks usually produce an effluent of better quality and seem to be less influenced by denitrification than the new ones.

Study on Activated Sludge Microorganisms of Northern Winter Sewage Treatment Plant

2013 ◽  
Vol 361-363 ◽  
pp. 1032-1035
Author(s):  
Xi Tian ◽  
Sheng Shu Ai ◽  
Yan Zuo ◽  
Qing Kai Ren ◽  
Zhong Yan Xie
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Activated Sludge ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Sharp Edge ◽  
Northern Winter ◽  
Autumn And Winter ◽  
Scanning Electron

At the sewage temperatures of 7-15°C, it used biological video microscopy and scanning electron microscopy to detect activated sludge morphology, miniature animals and flora species in northern winter sewage treatment plant. The results showed that activated sludge had dense floc with sharp edge, and was bigger at the temperatures of 13 and 15°C than at the temperatures of 7, 9 and 11°C. When the autumn and winter seasons were alternate and the sewage temperature was instability, the edge of the floc was slightly fuzzy and there was a small amount of free bacteria in activated sludge floc. The study had also shown that the temperature was higher, the type of sludge miniature animal and flora species were richer.

The Research of Biological Method Degradation on Cooking Fume

2012 ◽  
Vol 573-574 ◽  
pp. 1106-1111
Author(s):  
Wen Wan Meng ◽  
Lei Liao ◽  
Fei Chao Zhao ◽  
Zhen Zhou Wei
Keyword(s):  
Activated Sludge ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Oxidation Ditch ◽  
Whole Process ◽  
Cooking Fumes ◽  
Cooking Fume ◽  
Oil Concentration ◽  
Degradation Ability

Experiments according to the current domestic situation of bio-treatment cooking fume, domesticated the activated sludge which taken from the oxidation ditch of the sewage treatment plant by using the simulated cooking fumes, discussed the concentration and morphology variation of activated sludge, researched the degradation ability of microorganism on cooking fume, the change of bacteria amount were studied in the whole process. Research showed that the sludge concentration decreased to 2878mg/L when the acclimatization time reached 50days; outlet and inlet oil concentration were consistent when the acclimatization time reached 30days while liquid fume concentration decreased to 8.436mg/L, the degradation rate of activated sludge on liquid fume was about 75% after 30 days; Microbial biomass increased first and then down become stable finally.

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