scholarly journals Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1100
Author(s):  
Wioletta Parys ◽  
Małgorzata Dołowy ◽  
Alina Pyka-Pająk
Keyword(s):  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Residue Analysis ◽  
Food Products ◽  
Vital Role ◽  
Bioactive Molecules ◽  
Validation Parameters ◽  
Layer Chromatography ◽  
Natural And Synthetic

The present study summarizes the new strategies including advanced equipment and validation parameters of liquid and gas chromatography methods i.e., thin-layer chromatography (TLC), column liquid chromatography (CLC), and gas chromatography (GC) suitable for the identification and quantitative determination of different natural and synthetic bioactive compounds present in food and food products, which play an important role in human health, within the period of 2019–2021 (January). Full characteristic of some of these procedures with their validation parameters is discussed in this work. The present review confirms the vital role of HPLC methodology in combination with different detection modes i.e., HPLC-UV, HPLC-DAD, HPLC-MS, and HPLC-MS/MS for the determination of natural and synthetic bioactive molecules for different purposes i.e., to characterize the chemical composition of food as well as in the multi-residue analysis of pesticides, NSAIDs, antibiotics, steroids, and others in food and food products.

Thin-Layer Chromatography for Chlorinated Pesticide Residue Analysis without Cleanup

1964 ◽  
Vol 47 (2) ◽  
pp. 306-310
Author(s):  
H V Morley ◽  
M Chiba
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extracts ◽  
Residue Analysis ◽  
Rapid Screening ◽  
Pesticide Residue Analysis ◽  
Cleanup Procedure ◽  
Layer Chromatography

Abstract Thin-layer chromatography on silica gel may be used for the determination of chlorinated pesticide residues in some plant extracts without prior cleanup, thus eliminating the possible loss of pesticides during the cleanup procedure. Rapid screening of a large number of samples is thus possible with consequent saving of time and labor. When necessary, thin-layer chromatography may be used as a prior cleanup for gas chromatography.

Residue Analysis of Organophosphorus and Organochlorine Pesticides in Fatty Matrices by Gas Chromatography Coupled with Electron-Capture Detection

2006 ◽  
Vol 61 (5-6) ◽  
pp. 341-346 ◽  
Author(s):  
Jae-Woo Park ◽  
A. M. Abd El-Aty ◽  
Myoung-Heon Lee ◽  
Sung-Ok Song ◽  
Jae-Han Shim
Keyword(s):  
Gas Chromatography ◽  
Electron Capture ◽  
Simple Procedure ◽  
Linear Regression Analysis ◽  
Residue Analysis ◽  
Electron Capture Detection ◽  
Relative Standard ◽  
Wide Range ◽  
Repeatability And Reproducibility

A multiresidue method for the simultaneous determination of 22 organochlorine (OCs) and organophosphorus (Ops) pesticides (including isomers and metabolites), representing a wide range of physicochemical properties, was developed in fatty matrices extracted from meat. Pesticides were extracted from samples with acetonitrile/n-hexane (v :v, 1:1). The analytical screening was performed by gas chromatography coupled with electron-capture detection (ECD). The identification of compounds was based on their retention time and on comparison of the primary and secondary ions. The optimized method was validated by determining accuracy (recovery percentages), precision (repeatability and reproducibility), and sensitivity (detection and quantitation limits) from analyses of samples fortified at 38 to 300 ng/g levels. Correlation coefficients for the 22 extracted pesticide standard curves (linear regression analysis, n = 3) ranged from 0.998 to 1.000. Recovery studies from 2 g samples fortified at 3 levels demonstrated that the GC-ECD method provides 64.4-96.0% recovery for all pesticides except 2,4′-DDE (44.6-50.4%), 4,4′-DDE (51.1-57.5%) and 2,4′-DDT (50.0-51.2%). Both repeatability and reproducibility relative standard deviation values were < 20% for all residues. Detection limits ranged from 0.31 to 1.27 ng/g and quantification limits were between 1.04 and 4.25 ng/g. The proposed analytical method may be used as a simple procedure in routine determinations of OCs and Ops in meat. It can also be applied to the determination of pesticide multi-residues in other animal products such as butter and milk.

Determination of multiple organochlorine pesticide residues in shrimp using modified QuEChERS extraction and gas chromatography

2018 ◽  
Vol 16 (1) ◽  
pp. 81-93
Author(s):  
MDH Prodhan ◽  
SN Alam
Keyword(s):  
Gas Chromatography ◽  
Organochlorine Pesticide ◽  
Pesticide Residues ◽  
Limit Of Detection ◽  
Residue Analysis ◽  
Limit Of Quantification ◽  
Modified Quechers ◽  
Endosulfan Sulphate ◽  
Organochlorine Pesticide Residues

Determination of organochlorine pesticide residues in shrimp is very important to ensure the consumer’s safety and to fulfill the importer’s demand. Therefore, a simple and efficient multiple organochlorine pesticide residues analytical method using quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction technique and Gas Chromatography coupled with Electron Capture Detector (ECD) has been developed and validated for the determination of 19 organochlorine pesticides (α- BHC, δ- BHC, β- BHC, γ- BHC, Heptachlor, Aldrin, Heptachlor Epoxide, γ- Chlordane, α- Chlordane, α- Endosulfan, 4,4 DDE, Dieldrin, Endrin, 4,4 DDD, β- Endosulfan, 4,4 DDT, Endosulfan sulphate, Methoxychlor, and Endrin Ketone) in shrimp. The method was validated by evaluating the accuracy, precision, linearity, limit of detection (LOD) and limit of quantification (LOQ). The average recoveries of the selected pesticides ranged from 84% to 106% with RSDr ≤ 14% in four fortification levels of 0.05, 0.1, 0.2 and 0.3 mg kg-1. The linearity was ≥ 0.996 for all of the selected pesticides with matrix matched calibration standards. The LOD ranged from 0.003 to 0.009 mg kg-1 and the LOQ was 0.05 mg kg-1. This method was applied successfully for the residue analysis of 40 shrimp samples collected from different regions in Bangladesh.SAARC J. Agri., 16(1): 81-93 (2018)

Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography

2017 ◽  
Vol 54 (3) ◽  
pp. 294
Author(s):  
Hari Ramakrishnan K. ◽  
Janaky Ranjithkumar
Keyword(s):  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Highly Sensitive ◽  
Layer Chromatography

Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

Rapid Semimicro Method for the Determination of Methyl Mercury in Fish Tissue

1972 ◽  
Vol 55 (3) ◽  
pp. 583-589 ◽  
Author(s):  
J F Uthe ◽  
J Solomon ◽  
B Grift
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Methyl Mercury ◽  
Total Mercury ◽  
Aqueous Ethanol ◽  
Fish Tissue ◽  
Mercuric Iodide ◽  
Mercury In Fish ◽  
Layer Chromatography

Abstract A fast semimicro method for the determination of methyl mercury in fish tissue is described. The procedure involves extracting the methyl mercury into toluene as methyl mercuric bromide, partitioning the bromide into aqueous ethanol as a thiosulfate complex, and re-extracting into benzene as methyl mercuric iodide. Methyl mercury is quantitated with gas chromatography. The method is sensitive to 0.01 ppm. Recoveries of added methyl mercury were 99% and the presence of methyl mercury in the final extract was shown by thin layer chromatography and gas chromatography of the thin layer spot. A variety of mercurial compounds do not interfere in the analyses. The amounts of both methyl and total mercury found in a variety of tissues of aquatic animals are compared. The presence of a demethylase in seal is suggested by the findings of high levels of nonmethyl mercury. Additional cleanup by column chromatography on Florisil was necessary with certain samples. The gas chromatographic columns were kept operational by the intermittent injection of 3M potassium iodide. Due to column bleed and resulting detector contamination, the use of the easily cleaned concentric tube electron capture detector is recommended.

scholarly journals Recent Advances in the Thin-Layer Chromatography of Pesticides: A Review

2003 ◽  
Vol 86 (3) ◽  
pp. 602-611 ◽  
Author(s):  
Joseph Sherma
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Environmental Samples ◽  
High Performance ◽  
Residue Analysis ◽  
Thin Layer Chroma ◽  
Biological And Environmental Samples ◽  
Layer Chromatography ◽  
Related Compounds

Abstract Advances in the applications of thin-layer chroma-tography (TLC) and high-performance TLC for the separation, detection, identification, and determination of pesticides, other agrochemicals, and related compounds are reviewed for the period 2000–2002. Analyses are described for a variety of samples, such as food, biological, and environmental samples, and for residues of pesticides of various types, including insecticides, herbicides, and fungicides, belonging to different chemical classes. References are included for residue analysis, hydrophobicity studies, and the use of TLC and thin-layer radiochromatography for studies of pesticide metabolism, degradation, uptake, and related topics.

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