scholarly journals Ecofriendly Simple UV Spectrophotometric and Chemometric Methods for Simultaneous Estimation of Paracetamol Aceclofenac and Eperisone Hydrochloride in Pharmaceutical Formulation: Assessment of Greenness Profile

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1272
Author(s):  
Seetharaman Rathinam ◽  
Lakshmi Karunanidhi Santhana
Keyword(s):  
Principal Component Regression ◽  
Principal Component ◽  
Cost Effective ◽  
Simultaneous Equation ◽  
Uv Spectra ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Chemometric Methods ◽  
Spectrophotometric Methods ◽  
Significant Difference

This work introduces three eco-friendly UV spectrophotometric methods for the simultaneous estimation of Paracetamol, Aceclofenac and Eperisone Hydrochloride in pharmaceutical tablet formulation. The procedures employed were simultaneous equation method and multivariate chemometric methods with phosphate buffer pH 7.80 as diluent. The simultaneous equation method encompasses absorbance measurement at three different wavelengths (λmax of the drugs). It exhibits linearity between 12–18 µg mL−1 for paracetamol, 3.69–5.53 µg mL−1 for Aceclofenac, and 2.76–4.15 µg mL−1 Eperisone hydrochloride. The results obtained for accuracy and precision by the simultaneous equation method were within the permissible limits. Principal component regression and partial least squares were the tools used for chemometric methods. The calibration set and prediction set were constructed, and the UV spectra were recorded in zero order mode, further subjected to chemometric analysis. The % recoveries obtained for Paracetamol, Aceclofenac, and Eperisone Hydrochloride by chemometric techniques showed good accuracy, and the results obtained for analytical figures of merit were acceptable. Statistical comparison of the assay results obtained for the proposed methods showed no significant difference found among the methods using one way analysis of variance. Greenness evaluation tools revealed the greenness profile of the proposed methods and found them to be ecofriendly. The described methods were appropriate for routine quality control laboratories, facilitating eco-friendly, fast, and cost effective determination of Paracetamol, Aceclofenac, and Eperisone Hydrochloride in Acemyoset P tablets.

scholarly journals Liquid Chromatography and Chemometric-Assisted Spectrophotometric Methods for the Analysis of Two Multicomponent Mixtures Containing Cough Suppressant Drugs

2005 ◽  
Vol 88 (4) ◽  
pp. 1069-1080 ◽  
Author(s):  
Alaa El-Gindy ◽  
Samy Emara ◽  
Mostafa K Mesbah ◽  
Ghada M Hadad
Keyword(s):  
Liquid Chromatography ◽  
Principal Component Regression ◽  
Principal Component ◽  
Uv Spectra ◽  
Chemometric Methods ◽  
Multicomponent Mixtures ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Spectrophotometric Methods ◽  
Acid Sodium Salt

Abstract Three methods were applied for the analysis of 2 multicomponent mixtures containing dextromethorphan hydrobromide, phenylephrine hydrochloride, chlorpheniramine maleate, methylparaben, and propylparaben, together with either sodium benzoate (Mix 1) or ephedrine hydrochloride and benzoic acid (Mix 2). In the first method, liquid chromatography was used for their simultaneous determination using an ODS column with a mobile phase consisting of acetonitrile–phosphate buffer, pH 2.7 (40 + 60, v/v), containing 5mM heptanesulfonic acid sodium salt and ultraviolet (UV) detection at 214 nm. Also, 2 chemometric methods, principal component regression, and partial least squares were used. For both chemometric calibrations, a concentration set of the mixture consisting of each compound in each mixture was prepared in distilled water. The absorbance data in the UV spectra were measured for the 76 or 71 wavelength points in the spectral region 210–240 or 210–224 nm considering the intervals of Δλ = 0.4 or 0.2 nm for Mix 1 and Mix 2, respectively. The 2 chemometric methods did not require any separation step. These methods were successfully applied for the analysis of the 2 multicomponent combinations in synthetic mixtures and in commercial syrups, and the results were compared with each other.

scholarly journals Simultaneous Spectrophotometric Estimation and Validation of Domperidone, Tramadol Hydrochloride and Acetaminophen in Tablet Dosage Form

2011 ◽  
Vol 3 (1) ◽  
pp. 28-33
Author(s):  
Vikas Jain ◽  
Rajesh Sharma
Keyword(s):  
Component Analysis ◽  
Solvent Mixture ◽  
Simultaneous Equation ◽  
Pharmaceutical Products ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Tramadol Hydrochloride ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Tablet Dosage

Simultaneous estimation of active ingredients in multi-component pharmaceutical products normally requires the use of separation techniques, such as HPLC, HPTLC or GC, followed by their quantitation. Presented here are two spectrophotometric methods that do not require prior separation for simultaneous estimation of three drugs; acetaminophen, tramadol hydrochloride and domperidone in a tablet formulation. Shimadzu UV 1700 capable of multi-component analysis was used for quantitation. Method A is based on the simultaneous equation and method B on the multi-component analysis. The absorption maxima of the drugs found to be at 244nm, 271.5nm and 284.5nm respectively for acetaminophen, tramadol hydrochloride and domperidone in methanol/0.1 N HCl (1:2) solvent mixture. Acetaminophen, tramadol hydrochloride and domperidone obeyed Beer's law in the concentration range of 2-22 μg/ml, 10-55 μg/ml and 30-300 μg/ml respectively. The simultaneous equation method is based on the additivity of absorbances and multi-component analysis involves recording of absorbances of standard solutions at 244nm, 250nm, 271.5nm and 284.5nm. These were processed by means of statistical calculations and results of sample solution were obtained. Result of analysis for both methods were tested and validated for various parameters according to ICH guidelines. Key Words: simultaneous equation method; multicomponent analysis; acetaminophen; tramadol hydrochloride; domperidone. DOI: 10.3329/sjps.v3i1.2655S. J. Pharm. Sci. 3(1): 28-33

APPLICATION OF CHEMOMETRIC METHODS FOR DETERMINATION OF CLOTRIMAZOLE AND BECLOMETHASONE DIPROPIONATE IN PHARMACEUTICAL DOSAGE FORM

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (02) ◽  
pp. 45-50
Author(s):  
Umang Shah ◽  
Bhumika Desai ◽  
Vyomesh Nandrubarkar
Keyword(s):  
Beclomethasone Dipropionate ◽  
Principal Component Regression ◽  
Principal Component ◽  
Quality Parameters ◽  
Uv Spectra ◽  
Chemical Information ◽  
Chemometric Methods ◽  
Validation Data ◽  
Pharmaceutical Dosage Form

Chemometry is the use of mathematical and statistical methods to improve the understanding of chemical information and to correlate quality parameters or physical properties to analytical instrument data. In the present work, two chemometric methods, named as principal component regression (PCR) and (PLS) based on the use of spectrophotometric data, were developed for simultaneous determination of clotrimazole (CLO) and beclomethasone dipropionate (BE C) in bulk and cream form. The absorbance of zero order UV spectra of CLO and BE C in the range of 80-400 μg/mL and 2-10 μg/mL, respectively were recorded in the wavelength range 230-272 nm at 3 nm wavelength intervals. Twenty-five (25) mixed solutions were prepared for the chemometric calibration as training set and sixteen varied solutions were prepared as a validation set. The suitability of the models was decided based on the RMSECV, RMSEP and PRESS values of calibration and validation data. The % recovery study of both the methods was compared, and it was found near each other. The assay of CLO and BE C for both the methods was found to be in the range of 99.78 to 101.20%. Hence, the proposed methods can be used for simultaneous analysis of the mixture of the drugs, without chemical pre-treatment, with good speed of analysis.

SIMULTANEOUS ESTIMATION OF DAPAGLIFOZIN AND SAXAGLIPTIN IN TABLETS USING TWO CHEMOMETRIC-ASSISTED UV-SPECTROPHOTOMETRIC METHODS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (03) ◽  
pp. 32-38
Author(s):  
S. S Sonawane ◽  
S. S More ◽  
S. S. Chhajed ◽  
S. J. Kshirsagar ◽  
Keyword(s):  
Linear Regression ◽  
Phosphate Buffer ◽  
Principal Component Regression ◽  
Principal Component ◽  
Simultaneous Estimation ◽  
Training Set ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Validation Set

Two simple, accurate, precise and economical UV spectrophotometric methods, Multiple Linear Regression (MLR) and Principal Component Regression (PCR), were developed for the simultaneous estimation of dapaglifozin (DAPA) and saxagliptin (SAXA) in tablets. Beer’s law was obeyed in the concentration ranges of 10 – 50 μg/mL for DAPA and 5 – 25 μg/mL for SAXA. Synthetic mixtures containing two drugs were prepared to build the training set and validation set in the calibration range using D-optimal mixture design in phosphate buffer pH 6.8 and were recorded at six wavelengths in the range of 230 – 215 nm at intervals of Δλ = 3 nm. Both methods were validated as per ICH guidelines with respect to the accuracy and precision and found suitable for routine analysis of tablets containing DAPA and SAXA without separation.

scholarly journals Simultaneous Spectrophotometric Determination of Elbasvir and Grazoprevir in a Pharmaceutical Preparation

2018 ◽  
Vol 101 (2) ◽  
pp. 394-400 ◽  
Author(s):  
Khalid A M Attia ◽  
Nasr M El-Abasawi ◽  
Ahmed El-Olemy ◽  
Ahmed H Abdelazim
Keyword(s):  
Pharmaceutical Preparation ◽  
Principal Component Regression ◽  
Predictive Ability ◽  
Principal Component ◽  
Spectra Analysis ◽  
Pharmaceutical Dosage Form ◽  
Manufacturing Method ◽  
Spectrophotometric Methods ◽  
Significant Difference

Abstract Three UV spectrophotometric methods have been developed for the simultaneous determination of two new Food and Drug Administration-approved drugs, elbasvir (EBV) and grazoprevir (GRV), in their combined pharmaceutical dosage form. These methods include dual wavelength (DW), classic least-squares (CLS), and principal component regression (PCR). To achieve the DW method, two wavelengths were chosen for each drug in a way to ensure the difference in absorbance was zero from one drug to the other. GRV revealed equal absorbance at 351 and 315 nm, for which the distinctions in absorbance were measured for the determination of EBV. In the same way, distinctions in absorbance at 375 and 334.5 nm were measured for the determination of GRV. Alternatively, the CLS and PCR models were applied to the spectra analysis because the synchronous inclusion of many unreal wavelengths rather than using a single wavelength greatly increased the precision and predictive ability of the methods. The proposed methods were successfully applied to the assay of these drugs in their pharmaceutical formulation. The obtained results were statistically compared with manufacturing methods. The results conclude that there was no significant difference between the proposed methods and the manufacturing method with respect to accuracy and precision.

Comparative Study Between Multivariate and Univariate Analysis of Two Antidiabetic Combinations

2017 ◽  
Vol 100 (5) ◽  
pp. 1379-1391 ◽  
Author(s):  
Maha F Abdel-Ghany ◽  
Omar Abdel-Aziz ◽  
Miriam F Ayad ◽  
Mariam M Tadros
Keyword(s):  
Mobile Phase ◽  
Univariate Analysis ◽  
Principal Component Regression ◽  
Principal Component ◽  
Zero Order ◽  
Spectrophotometric Methods ◽  
Chromatographic Techniques ◽  
Mean Centering ◽  
Significant Difference

Abstract New multivariate and univariate methods were developed for the analysis of two novel gliptin combinations by manipulating the zero-order and ratio spectra of empagliflozin and linagliptin in combination, with application on Glyxambi® tablets, and of alogliptin and pioglitazone in combination, with application on Oseni® tablets. Linearity ranges for chemometric approaches using principal component regression and partial least-squares were found to be 2–10, 2.5–12.5, 5–15, and 5–25 μg/mL for empagliflozin, linagliptin, alogliptin, and pioglitazone, respectively, whereas the respective linearity ranges for the spectrophotometric approaches were found to be 5–15, 2–12, 5–15, and 5–15 μg/mL. The proposed spectrophotometric methods included ratio subtraction coupled withextended ratio subtraction, spectrum subtraction coupled with constant multiplication, and mean centering. Acceptable LOD and LOQ values were obtained by all methods. Statistical analysis showed no significant difference between multivariate and univariate methods in comparison with the reference methods. The optimized methods provide fast and economic determination of the recently approved antidiabetic combinations without the complex instrumentation or time-consuming mobile phase preparations that were used in the chromatographic techniques reported in the literature.

scholarly journals SIMULTANEOUS UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF CEFADROXIL AND PROBENECID IN TABLET DOSAGE FORM

2018 ◽  
Vol 1 (3) ◽  
pp. 19-23
Author(s):  
Mayur S. Jain ◽  
Sunil R. Bavaskar ◽  
Shashikant D. Barhate ◽  
Jintendra D. Fegade
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Spectrophotometric Methods ◽  
Absorption Ratio ◽  
Tablet Dosage

Two methods for simultaneous estimation of Cefadroxil and Probenecid in combined tablet dosage form have been developed. The first UV spectrophotometric method was a determination using the simultaneous equation method at 233 nm and 247 nm. The second UV spectrophotometric method is the Q – analysis (absorption ratio) method, which involves the formation of absorbance equation at 242 nm (Isobestic point) and at 247 nm the maximum absorption of Probenecid . The linearity ranges for Cefadroxil and Probenecid both were 10-60μg/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 99.43±0.75 and 99.69±0.40 for simultaneous equation method and 99.23±0.34 and 99.56±0.16 for absorption ratio method for Cefadroxil and Probenecid respectively. These methods are simple, accurate and rapid; those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF VILANTEROL AND FLUTICASONE FUROATE IN PHARMACEUTICAL FORMULATIONS

2017 ◽  
Vol 10 (4) ◽  
pp. 302
Author(s):  
Siva Kishore Masimukku ◽  
Rambabu Chintal
Keyword(s):  
Method Development ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Fluticasone Furoate ◽  
Limit Of Quantification ◽  
Spectrophotometric Methods ◽  
Development And Validation

Objective:  To develop a simple, rapid,  precise, accurate, sensitive spectrophotometric methods (A&B) were developed for simultaneous estimation and validation of Vilanterol (VTL) and Fluticasone Furoate (FFE) in pure and tablet dosage forms.Method:   Method A is a simultaneous equation method and method B is a first order derivative spectrophotometric method. Pure drug samples of VTL and FFE were dissolved in a mixture of Methanol and Ethanol in the ratio of 1:1 (v/v) and found to have absorbance maxima at 231nm for VTL and 260nm for FFE respectivelyResults:  The linearity lies between 2.5–10µg/ml for VTL and 10–60µg/ml for FFE in these two methods (A&B).  The correlation coefficient (r2) was found to be 0.999 for both VTL and FFE, the limit of detection and limit of quantification were found to be 0.015µg/ml and 0.05µg/ml for VTL and 0.05µg/ml and 0.2µg/ml for FFE respectively. The results of analysis have been validated statistically by recovery studies as per ICH guidelines.Conclusion: The two methods A&B showed good reproducibility and recovery with % RSD less than 2.  Hence both methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of VTL and FFE in pure and combined dosage form.Keywords: Fluticasone furoate, Vilanterol, Derivative spectrophotometric, Simultaneous equation method, Method development and validation.

scholarly journals Novel Stability-Indicating Chemometric-Assisted Spectrophotometric Methods for the Determination of Chlordiazepoxide and Clidinium Bromide in the Presence of Clidinium Bromide’s Alkali-Induced Degradation Product

2018 ◽  
Vol 101 (3) ◽  
pp. 714-722 ◽  
Author(s):  
Christine K Nessim ◽  
Adel M Michael ◽  
Yasmin M Fayez ◽  
Hayam M Lotfy
Keyword(s):  
Degradation Product ◽  
Multivariate Calibration ◽  
Principal Component Regression ◽  
Principal Component ◽  
Uv Spectra ◽  
Commercial Formulation ◽  
Spectrophotometric Methods ◽  
Calibration Models ◽  
Clidinium Bromide

Abstract Two simple and accurate chemometric-assisted spectrophotometric models were developed and validated for the simultaneous determination of chlordiazepoxide (CDZ) and clidinium bromide (CDB) in the presence of an alkali-induced degradation product of CDB in their pure and pharmaceutical formulation. Resolution was accomplished by using two multivariate calibration models, including principal component regression (PCR) and partial least-squares (PLS), applied to the UV spectra of the mixtures. Great improvement in the predictive abilities of these multivariate calibrations was observed. A calibration set was constructed and the best model used to predict the concentrations of the studied drugs. CDZ and CDB were analyzed with mean accuracies of 99.84 ± 1.41 and 99.81 ± 0.89% for CDZ and 99.56 ± 1.43 and 99.44 ± 1.41% for CDB using PLS and PCR models, respectively. The proposed models were validated and applied for the analysis of a commercial formulation and laboratory-prepared mixtures. The developed models were statistically compared with those of the official and reported methods with no significant differences observed. The models can be used for the routine analysis of both drugs in QC laboratories.

scholarly journals Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Furosemide and Spironolactone by Vierordt’s Method in Bulk and Combined Tablet Dosage Form

2018 ◽  
Vol 26 (1) ◽  
pp. 74-90 ◽  
Author(s):  
Rohankumar R. Chavan ◽  
Somnath D. Bhinge ◽  
Mangesh A. Bhutkar ◽  
Dheeraj S. Randive
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Limit Of Detection ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Bulk Drug

Abstract Anew simple, convenient and suitable spectrophotometric method for simultaneous determination of Furosemide and Spironolactone in combined dosage form has been developed and validated. Simultaneous equation method (Vierordt’s method) was used for determination of Furosemide and Spironolactone in combined dosage form. For spectrophotometric method development double distilled water and ethanol were used as a solvent in the ratio of (20:80). The proposed method was quantitatively evaluated in terms of linearity, precision, accuracy, lower limit of detection (LOD) and quantification (LOQ), recovery and robustness. All the parameters were found to be within the acceptance limit. λmax of Furosemide and Spironolactone was found to be 275 and 237 nm respectively. Beer’s law was obeyed over the concentration ranges of 2-10 μg mL−1 for both Furosemide and Spironolactone respectively. The % assay for commercial formulation was found to be 99.60%±0.0500 for Furosemide and 100.26%±1.17 for Spironolactone by the proposed methods. The overall recovery was observed to be 100.38±0.09% for Furosemide and 100.49±0.4197% for Spironolactone by simultaneous equation method (Vierordt’s method). LOD and LOQ were 0.76 and 2.32 μg mL−1 for Furosemide, 1.99 and 6.04 μg mL−1 for Spironolactone. A new simple, convenient, precise, rapid, accurate and economical and reliable spectrophotometric method was developed and validated for the analysis of Furosemide and Spironolactone in bulk drug and their formulations.

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