Improved Production of Kynurenic Acid by Yarrowia lipolytica in Media Containing Different Honeys

Y. lipolytica remains a nonpathogenic, unconventional yeast, which can be applied for the production of bioactive compounds. Our previous study confirmed the ability of yeast Yarrowia lipolytica to produce kynurenic acid (KYNA). Here, we investigated the effectiveness of KYNA production in cultures cultivated in medium containing honey of various origin, used as a source of carbon and energy. It was evidenced that the highest content of KYNA in culture broth (68 mg/L) and yeast biomass (542 mg/kg) was obtained when chestnut honey was used. The content of lipids and amino acids composition in yeast biomass producing KYNA was also determined. It was found that the composition of both amino acids and lipids in yeast biomass depended on the honey type used as a component of the medium. This finding revealed that supplementation of medium broth with honey may significantly affect the nutritional value of yeast biomass. The practical applicability of this finding requires further study.

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The peroxin Pex17p of the yeast Yarrowia lipolytica is associated peripherally with the peroxisomal membrane and is required for the import of a subset of matrix proteins.

Molecular and Cellular Biology ◽

10.1128/mcb.17.5.2511 ◽

1997 ◽

Vol 17 (5) ◽

pp. 2511-2520 ◽

Cited By ~ 30

Author(s):

J J Smith ◽

R K Szilard ◽

M Marelli ◽

R A Rachubinski

Keyword(s):

Amino Acids ◽

Oleic Acid ◽

Yarrowia Lipolytica ◽

Matrix Proteins ◽

Peroxisomal Membrane ◽

Peroxisomal Targeting ◽

Targeting Signal ◽

Carboxyl Terminal ◽

Peroxisomal Targeting Signal ◽

Yeast Yarrowia Lipolytica

PEX genes encode peroxins, which are required for the biogenesis of peroxisomes. The Yarrowia lipolytica PEX17 gene encodes the peroxin Pex17p, which is 671 amino acids in length and has a predicted molecular mass of 75,588 Da. Pex17p is peripherally associated with the peroxisomal membrane. The carboxyl-terminal tripeptide, Gly-Thr-Leu, of Pex17p is not necessary for its targeting to peroxisomes. Synthesis of Pex17p is low in cells grown in glucose-containing medium and increases after the cells are shifted to oleic acid-containing medium. Cells of the pex17-1 mutant, the original mutant strain, and the pex17-KA mutant, a strain in which most of the PEX17 gene is deleted, fail to form normal peroxisomes but instead contain numerous large, multimembraned structures. The import of peroxisomal matrix proteins in these mutants is selectively impaired. This selective import is not a function of the nature of the peroxisomal targeting signal. We suggest a regulatory role for Pex17p in the import of a subset of matrix proteins into peroxisomes.

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Kanatlı Beslenmesinde Dut Yaprağının (Morus alba) Kullanılması

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v4i6.504-507.682 ◽

2016 ◽

Vol 4 (6) ◽

pp. 504

Author(s):

Tugay Ayasan ◽

Mikail Baylan

Keyword(s):

Amino Acids ◽

Nutritional Value ◽

Fish Meal ◽

Essential Amino Acids ◽

Metabolic Energy ◽

Good Source ◽

Mulberry Leaf ◽

Amino Acids Composition ◽

Poultry Diets ◽

The Tropics

The increasing costs of conventional feedstuffs like corn, soybean meal and fish meal for poultry diets is pushing the need to find less expensive alternatives. Mulberry grows well in the tropics and subtropics, and is reported to have excellent nutritional value. Mulberry leaves are very rich in protein (15-35%), minerals (2.42-4.71% Ca, 0.23-0.97% P), 1130-2240 kcal/kg metabolic energy (ME). The amino acids composition of mulberry leaf meal indicates it is a good source of essential amino acids especially lysine (1.80%) and leucine (2.58%). In this paper, studies made on the mulberry leaf used in nutrition of poultries have been reviewed.

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Rye and Oat Agricultural Wastes as Substrate Candidates for Biomass Production of the Non-Conventional Yeast Yarrowia lipolytica

Sustainability ◽

10.3390/su12187704 ◽

2020 ◽

Vol 12 (18) ◽

pp. 7704

Author(s):

Katarzyna Drzymała ◽

Aleksandra Maria Mirończuk ◽

Witold Pietrzak ◽

Adam Dobrowolski

Keyword(s):

Yarrowia Lipolytica ◽

Low Cost ◽

Agricultural Waste ◽

Sugar Content ◽

Total Yield ◽

Dry Weight ◽

Cell Protein ◽

Yeast Biomass ◽

Oat Bran ◽

Yeast Yarrowia Lipolytica

The aim of this study was to test rye straw, rye bran and oat bran hydrolysates as substrates for growth of the yeast Yarrowia lipolytica, a microorganism known to have large biotechnological potential. First, after the combined process of acid-enzymatic hydrolysis, the concentration and composition of fermentable monosaccharides in the obtained hydrolysates were analyzed. Glucose was the main sugar, followed by xylose and arabinose. Rye bran hydrolysate had the highest sugar content—80.8 g/L. The results showed that this yeast was able to grow on low-cost medium and produce biomass that could be used as a feed in the form of single cell protein. The biomass of yeast grown in oat bran hydrolysate was over 9 g/L after 120 h, with the biomass total yield and total productivity values of 0.141 g/g and 0.078 g/h, respectively. The protein contents in yeast biomass were in the range of 30.5–44.5% of dry weight. Results obtained from Y. lipolytica cultivated in rye bran showed high content of exogenous amino acid (leucine 3.38 g, lysine 2.93 g, threonine 2.31 g/100 g of dry mass) and spectrum of unsaturated fatty acid with predominantly oleic acid—59.28%. In conclusion, these results demonstrate that lignocellulosic agricultural waste, after hydrolysis, could be efficiently converted to feed-related yeast biomass.

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Application of a New Engineered Strain of Yarrowia lipolytica for Effective Production of Calcium Ketoglutarate Dietary Supplements

International Journal of Molecular Sciences ◽

10.3390/ijms22147577 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7577

Author(s):

Ludwika Tomaszewska-Hetman ◽

Anita Rywińska ◽

Zbigniew Lazar ◽

Piotr Juszczyk ◽

Magdalena Rakicka-Pustułka ◽

...

Keyword(s):

Dietary Supplements ◽

Yarrowia Lipolytica ◽

Kynurenic Acid ◽

Nutritional Properties ◽

Yeast Biomass ◽

Genes Encoding ◽

Food And Feed ◽

Health Promoting ◽

Methylcitrate Synthase ◽

The Impact

The present study aimed to develop a technology for the production of dietary supplements based on yeast biomass and α-ketoglutaric acid (KGA), produced by a new transformant of Yarrowia lipolytica with improved KGA biosynthesis ability, as well to verify the usefulness of the obtained products for food and feed purposes. Transformants of Y. lipolytica were constructed to overexpress genes encoding glycerol kinase, methylcitrate synthase and mitochondrial organic acid transporter. The strains were compared in terms of growth ability in glycerol- and oil-based media as well as their suitability for KGA biosynthesis in mixed glycerol–oil medium. The impact of different C:N:P ratios on KGA production by selected strain was also evaluated. Application of the strain that overexpressed all three genes in the culture with a C:N:P ratio of 87:5:1 allowed us to obtain 53.1 g/L of KGA with productivity of 0.35 g/Lh and yield of 0.53 g/g. Finally, the possibility of obtaining three different products with desired nutritional and health-beneficial characteristics was demonstrated: (1) calcium α-ketoglutarate (CaKGA) with purity of 89.9% obtained by precipitation of KGA with CaCO3, (2) yeast biomass with very good nutritional properties, (3) fixed biomass-CaKGA preparation containing 87.2 μg/g of kynurenic acid, which increases the health-promoting value of the product.

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A Yarrowia lipolytica Strain Engineered for Pyomelanin Production

Microorganisms ◽

10.3390/microorganisms9040838 ◽

2021 ◽

Vol 9 (4) ◽

pp. 838

Author(s):

Macarena Larroude ◽

Djamila Onésime ◽

Olivier Rué ◽

Jean-Marc Nicaud ◽

Tristan Rossignol

Keyword(s):

Amino Acids ◽

Yarrowia Lipolytica ◽

De Novo ◽

Aromatic Amino Acids ◽

Acid Synthesis ◽

Homogentisic Acid ◽

Lipolytica Strain ◽

Acid Pathway ◽

Extracellular Environment ◽

Yeast Yarrowia Lipolytica

The yeast Yarrowia lipolytica naturally produces pyomelanin. This pigment accumulates in the extracellular environment following the autoxidation and polymerization of homogentisic acid, a metabolite derived from aromatic amino acids. In this study, we used a chassis strain optimized to produce aromatic amino acids for the de novo overproduction of pyomelanin. The gene 4HPPD, which encodes an enzyme involved in homogentisic acid synthesis (4-hydroxyphenylpyruvic acid dioxygenase), was characterized and overexpressed in the chassis strain with up to three copies, leading to pyomelanin yields of 4.5 g/L. Homogentisic acid is derived from tyrosine. When engineered strains were grown in a phenylalanine-supplemented medium, pyomelanin production increased, revealing that the yeast could convert phenylalanine to tyrosine, or that the homogentisic acid pathway is strongly induced by phenylalanine.

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Variability Russian Rice Varieties in Essential Amino Acids Composition

E3S Web of Conferences ◽

10.1051/e3sconf/202128502046 ◽

2021 ◽

Vol 285 ◽

pp. 02046

Author(s):

Yulia Goncharova ◽

Svetlana Verechagina ◽

Nikolay Ochkas ◽

Sergey Goncharov

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Amino Acid Composition ◽

Acid Composition ◽

Nutritional Value ◽

Protein Composition ◽

Essential Amino Acids ◽

Rice Varieties ◽

Amino Acids Composition ◽

White Grain

Rice contains antioxidants, minerals, carbohydrates, lipids, vitamins that make it a functional food. A dozen times higher content of antioxidants was noted in black-grain and red-grain rice varieties as compared to white-grain ones. In terms of protein content, color and white-grain varieties do not differ significantly. The protein composition largely determines the nutritional value and functional properties, as well as the texture and taste of rice. Although rice contains less protein than many cereals, its biological value is highest due to balanced composition of amino acids. Nutritional value of different rice varieties varies greatly due to different amino acid composition. Determination of amino acid composition was carried out on devices Kapel 105 - M. In the work used 50 collection samples of rice. The variability was studied and the sources were identified among domestic rice varieties. To create a new generation varieties with more balanced amino acid composition, it is necessary both to isolate sources with a high content of individual amino acids, and varieties with high characteristics in terms of content complex of amino acids, especially essential ones.

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Screening of amino acid constituents from date palm fruits

International Journal of Bioassays ◽

10.21746/ijbio.2016.10.0011 ◽

2016 ◽

Vol 5 (10) ◽

pp. 4972

Author(s):

Lata Birlangi

Keyword(s):

Amino Acids ◽

Amino Acid ◽

United Arab Emirates ◽

Nutritional Value ◽

Date Palm ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Cultivated Plants ◽

Palm Fruits ◽

Social Part

The date palm (Phoenix dactylifera L.) is one of mankind’s oldest cultivated plants. The fruit of the date palm is an important crop of the hot arid and semi-arid regions of the world. It has always played a genuine economic and social part in the lives of the people of these areas. The present objective in examining the amino acid content of different varieties of date palm fruits from Middle-East region; is to determine whether its protein could effectively supplement the nutritional value and it is also aimed in finding which variety is rich in number of amino acids. The phytochemical screening revealed the presence of eight essential amino acids and five non-essential amino acids in the date fruits. Among all the date fruit varieties taken as samples for the study, Dabbas cultivar of United Arab Emirates found to exhibit eight types of amino acids which includes five as non-essential ones. Total of thirteen amino acids were detected in the seven date cultivars. Determination of amino acid can serve as a guide to the possible nutritional value.

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QUANTITATIVE ANALYSIS OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. LEAF EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s2.13480 ◽

2016 ◽

pp. 183

Author(s):

Parameswari P ◽

Devika Rengaswamy

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Quantitative Analysis ◽

Secondary Metabolites ◽

Bioactive Compounds ◽

Leaf Extract ◽

Metabolic Reaction ◽

Quantitative Investigation ◽

Artemisia Nilagirica ◽

Quantitative Examination

ABSTRACT Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on different human diseases. Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments for quantitative investigation of phytochemicals according to standard strategies. Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows separately. Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward extraction and refinement of specific bioactive mixes for human nourishment. Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.

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