scholarly journals QUANTITATIVE ANALYSIS OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. LEAF EXTRACT

Author(s):  
Parameswari P ◽  
Devika Rengaswamy

<p>ABSTRACT<br />Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely<br />on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on<br />different human diseases.<br />Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments<br />for quantitative investigation of phytochemicals according to standard strategies.<br />Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg<br />terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows<br />separately.<br />Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward<br />extraction and refinement of specific bioactive mixes for human nourishment.<br />Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.</p>

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1669
Author(s):  
Yesica Vilcanqui ◽  
Luis Omar Mamani-Apaza ◽  
Marcos Flores ◽  
Jaime Ortiz-Viedma ◽  
Nalda Romero ◽  
...  

The southern coast of Peru presents a wide diversity of seaweed, which could be used as a new sustainable source of nutritional and bioactive compounds. For the first time, we chemically characterized two species of brown (Macrocystis pyrifera) and red (Chondracanthus chamissoi) Peruvian seaweed. Both species contained significant amounts of proteins (5–12%), lipids (0.16–0.74%), carbohydrates (43.29–62.65%) and minerals (1300–1800 mg kg−1 dw: dry weight). However, the profiles of amino acids, fatty acids and minerals were highly dependent on species type. C. chamissoi had a higher content of essential amino acids and minerals than M. pyrifera (170% and 45%, respectively), while the presence of polyunsaturated fatty acids (ω 6) as well as the content of tocopherols was higher in M. pyrifera (15.77 g 100 g−1 and 2.37 μg 100 g−1, respectively). Additionally, both species presented significant concentrations of total polyphenols (39–59 mg GAE g−1) and a high antioxidant capacity (67–98 µM TE g−1). Although M. pyrifera and C. chamissoi seem to be excellent raw materials for the food and nutraceutical industry, both species contained toxic heavy metals (cadmium: Cd and nickel: Ni) which could affect the safety of their direct use. Therefore, new separation strategies that allow the selective recovery of nutrients and bioactive compounds from Peruvian seaweed are required.


2017 ◽  
Vol 8 (8) ◽  
pp. 2672-2685 ◽  
Author(s):  
J. Matos ◽  
C. Cardoso ◽  
N. M. Bandarra ◽  
C. Afonso

Microalgae are very interesting and valuable natural sources of highly valuable bioactive compounds, such as vitamins, essential amino acids, polyunsaturated fatty acids, minerals, carotenoids, enzymes and fibre.


1981 ◽  
Vol 36 (6) ◽  
pp. 757-762 ◽  
Author(s):  
Hartmut Klein ◽  
Wittko Francke ◽  
Wilfried A. König

Abstract Low molecular weight constituents of 3400 venom sacs of Paravespula vulgaris were extracted with water, diluted acetic acid, and methanol. After conversion to volatile derivatives carbohydrates, amino acids, fatty acids, other constituents of phospho lipids, and biogenic amines were identified by gas chromatography and mass spectrometry. Quantitative analysis was achieved for amino acids by amino acid analysis and for glucose by an enzymatic assay. Results are discussed with regard to biosynthesis of wasp toxins and phylogenetic aspects of hymenopteran venoms.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Dwitiyanti Dwitiyanti ◽  
Yahdiana Harahap ◽  
Berna Elya ◽  
Anton Bahtiar

Patients with diabetes are 1.6 times more likely to use complementary alternative medicine than nondiabetic patients. Previous studies have shown that Anredera cordifolia (Tenore) Steen. (A. cordifolia) leaf extract has the capacity to lower blood glucose, but the actual mechanisms are unclear. Therefore, in this study, we explored the effect of A. cordifolia leaf extract on the metabolism of fatty acids and amino acids. Six-week-old male Wistar rats were randomly divided into six experimental groups (n = 5 per group). Two groups were fed with a regular diet or a high-fat diet (HFD) for six weeks. The regular diet and HFD groups were administered with 0.5% carboxymethylcellulose as a vehicle, and HFD rats were also fed with a suspension of glibenclamide (0.51 mg/kg body weight (BW)) or A. cordifolia leaf extract (25, 50, and 100 mg/kg BW). During the whole treatment, BW and food intake were recorded weekly. The rats were euthanized seven weeks after treatment. Blood glucose was evaluated by spectrophotometry, while fatty acids and amino acids were evaluated using a gas chromatography/flame ionization detector (GC/FID). All doses of A. cordifolia administration reduced blood glucose significantly, and 50 mg/kg BW was most effective in lowering blood glucose, similar to the effects of glibenclamide. A. cordifolia leaf extract affected the levels of medium-chain fatty acids, especially at 50 mg/kg BW. In contrast, glibenclamide affected long-chain fatty acids (LCFAs) to lower blood glucose. Based on the analysis conducted, we conclude that administration of A. cordifolia leaf extract can decrease blood glucose levels by regulating fatty acid metabolism and that a dose of 50 mg/kg BW in rats was the optimal dose.


2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2099-2103
Author(s):  
Kousalya P ◽  
Doss VA

The main goal of the research was to explore the existence of phytochemicals, quantification of primary and secondary metabolites of leaves extract of Artabotrys hexapetalus  (L.f.) Bhandari. The phytochemical activity of leaves of Artabotrys hexapetalus was assessed using different solvent extracts like water, ethanol, acetone, chloroform and petroleum ether. Among the different solvent extracts, aqueous leaves extract revealed the high content of phytochemicals. So the aqueous leaves extract was used for further investigations. Aqueous leaves extract of Artabotrys hexapetalus was subjected to quantitative analysis of primary metabolites like carbohydrates, proteins and amino acids. Quantitative analysis of secondary metabolites like flavonoids, tannins and phenols were performed using aqueous leaves extract of Artabotrys hexapetalus. Qualitative screening of phytochemicals reported the existence of carbohydrates, amino acids, proteins, flavonoids, alkaloids, saponins, phenols, glycosides, tannins and diterpenes. Quantitative analysis showed the presence of carbohydrates (43.16±1.0 mg/g extract), proteins (60.4±0.88 mg/g extract), amino acids (19.33 ± 1.30 mg/g extract), flavonoids (28.3 ±0.91 mg/g extract), tannins (24.53±1.02 mg/g extract) and phenols (7.63±0.85 mg/g extract). The present study concluded that aqueous leaves extract of Artabotrys hexapetalus as a potential source of phytochemicals, primary and secondary metabolites.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Rajani KS ◽  
Swasha Khandeparker Naik ◽  
Subhadarshini Satapathy ◽  
Sweta Mishra

The present study highlights preliminary phytochemical analysis of the leaf extract of Nyctanthes arbor-tristis. Plants produce a wide range of bioactive molecules which make them as a rich source of various types of medicines. Nyctanthes arbor-tristis is one of the well-known medicinal herbs of Indian origin belonging to family Oleaceae, commonly known as night Jasmine. The leaf extract is taken by percolation method. Various bioactive primary and secondary metabolites were tested using colour reactions with specific reagents. The study reveals that it contains some bioactive compounds which indicate that this plant might be used as anti-malarial, anti-viral and antidiabetic.


2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Hafiluddin, Nurjanah, Tati Nurhayati

Abstract One of the commodities produced from the ocean that have a bioactive compounds is a sea slug (Discodoris sp.). That is very interesting to study mainly deals with the nature of both chemical and biochemical characteristics and their use for the field of food and health. The aims of this research was to determine the nutrient content of sea slug, determaine chemical content and determine antioxidant activity. The experiment was conducted with several stages: sample preparation, analysis of nutrient content, extraction bioactive compound, analysis of chemical content and antioxidant activity.  Sea slug from the island of Madura Pamekasan potential as a source of protein, fat, and minerals. Sea slug has esensial amino acids amount to 5.57% was dominated by leucin of 1.42%. Nonesensial amino acids amount to 6.54% wich was dominated by glutamic acid 2.19%.  Saturated fatty acids amounted to 27.53% of sea slug was dominated by palmitic (C16: 0) which was 13.36%. Unsaturated fatty acids amounted to 34.66%, which was dominated by the essential fatty acids linolenic (C18: 3, n-3) 20.91%. The highest yield of the crude extract was ethanol and contained alkaloid compounds, steroids, phenols, carbohydrates and reducing sugar compound. The meat of sea slug with ethanol solvent has an IC best 50 antioxidant activity at 441, 12 ppm.


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