Plant-Microbe Synergism in Floating Treatment Wetlands for the Enhanced Removal of Sodium Dodecyl Sulphate from Water

Excessive use of detergents in wide industrial processes results in unwanted surfactant pollution. Among them, sodium dodecyl sulphate (SDS) has well-known history to be used in pharmaceutical and industrial applications. However, if discharged without treatment, it can cause toxic effects on living organisms especially to the aquatic life. Floating treatment wetlands (FTWs) could be a cost-effective and eco-friendly options for the treatment of wastewater containing SDS. In this study, FTWs mesocosms were established in the presence of hydrocarbons-degrading bacteria. Two plant species (Brachiaria mutica and Leptochloa fusca) were vegetated and a consortium of bacteria (Acinetobacter sp. strain BRSI56, Acinetobacter junii strain TYRH47, and Acinetobacter sp. strain CYRH21) was applied to enhance degradation in a short-time. Results illustrated that FTWs vegetated with both plants successfully removed SDS from water, however, bacterial augmentation further enhanced the removal efficiency. Maximum reduction in SDS concentration (97.5%), chemical oxygen demand (92.0%), biological oxygen demand (94.2%), and turbidity (99.4%) was observed in the water having FTWs vegetated with B. mutica and inoculated with the bacteria. The inoculated bacteria showed more survival in the roots and shoots of B. mutica as compared to L. fusca. This study concludes that FTWs have the potential for the removal of SDS from contaminated water and their remediation efficiency can be enhanced by bacterial augmentation.

Download Full-text

Investigating degradation metabolites and underlying pathway of azo dye “Reactive Black 5” in bioaugmented floating treatment wetlands

10.21203/rs.3.rs-351058/v1 ◽

2021 ◽

Author(s):

Nain Tara ◽

Mazhar Iqbal ◽

Fazale Habib ◽

Qaiser Mahmood Khan ◽

Samina Iqbal ◽

...

Keyword(s):

Azo Dyes ◽

Azo Dye ◽

Dye Degradation ◽

Treatment Wetlands ◽

Reactive Black 5 ◽

Acinetobacter Junii ◽

Degrading Bacteria ◽

Carcinogenic Effects ◽

Successful Removal ◽

Floating Treatment Wetlands

Abstract The direct discharge of azo dyes and/or their metabolites into the environment may exert toxic, mutagenic, and carcinogenic effects on exposed fauna and flora. In this study, we analyzed the metabolites produced during the degradation of an azo dye namely Reactive Black 5 (RB5) in the bacterial augmented-floating treatment wetlands (FTWs), followed by the investigation of their underlying toxicity. To this end, a FTWs system was developed by using a common wetland plant Phragmites australis in the presence of three dye-degrading bacteria (Acinetobacter junii strain NT-15, Pseudomonas indoloxydans strain NT-38, and Rhodococcus sp. strain NT-39). We found that the FTW system effectively degraded RB5 into at least 20 different metabolites with the successful removal of color (95.5%) from the water. The fish toxicity assay revealed the non-toxic characteristics of the metabolites produced after dye degradation. Our study suggests that bacterially aided FTWs could be a suitable option for the successful degradation of azo dyes, and the results presented in this study may help improve the overall textile effluent clean-up processes.

Download Full-text

Remediation of polluted river water by floating treatment wetlands

Water Science & Technology Water Supply ◽

10.2166/ws.2018.154 ◽

2018 ◽

Vol 19 (3) ◽

pp. 967-977 ◽

Cited By ~ 12

Author(s):

Munazzam Jawad Shahid ◽

Razia Tahseen ◽

Muhammad Siddique ◽

Shafaqat Ali ◽

Samina Iqbal ◽

...

Keyword(s):

River Water ◽

Oxygen Demand ◽

Treatment Wetlands ◽

Bacterial Strains ◽

Aquatic Life ◽

Polluted River ◽

Leptochloa Fusca ◽

Quality Guidelines ◽

Floating Treatment Wetlands ◽

Better Than

Abstract In this study, the potential of floating treatment wetlands (FTWs), inoculated with selected bacteria, to ameliorate polluted river water was evaluated. Floating cells were prepared by vegetating plants, Typha domingensis and Leptochloa fusca, on a floating mat. The plants were inoculated with three different pollutant-degrading rhizospheric and endophytic bacterial strains. Significantly greater decrease in chemical oxygen demand (COD), biochemical oxygen demand (BOD5) and total organic carbon (TOC) was observed in inoculated FTWs than in the wetlands without bacterial inoculation. However, a slight decrease in pH and EC was seen in most of the treatments. The total nitrogen (TN), nitrate and total phosphorus (TP) contents decreased to 1.77 mg/L, 0.80 mg/L and 0.60 mg/L, respectively. Additionally, the concentration of iron (Fe), nickel (Ni), manganese (Mn), lead (Pb), and chromium (Cr) in the water lowered to 0.41, 0.16, 0.10, 0.25, and 0.08 mg/L, respectively. Overall the performance of T. domingensis was significantly better than L. fusca. The treated effluents meet the water quality guidelines for irrigation and aquatic life. This study revealed that FTWs supplemented with selective bacteria are a promising approach for the restoration and management of polluted river water.

Download Full-text

Cyperus laevigatus L. Enhances Diesel Oil Remediation in Synergism with Bacterial Inoculation in Floating Treatment Wetlands

Sustainability ◽

10.3390/su12062353 ◽

2020 ◽

Vol 12 (6) ◽

pp. 2353 ◽

Cited By ~ 2

Author(s):

Muhammad Fahid ◽

Shafaqat Ali ◽

Ghulam Shabir ◽

Sajid Rashid Ahmad ◽

Tahira Yasmeen ◽

...

Keyword(s):

Bacterial Consortium ◽

Pilot Scale ◽

Diesel Oil ◽

Treatment Wetlands ◽

Contaminated Water ◽

Bacterial Strains ◽

Joint Application ◽

Degrading Bacteria ◽

Acinetobacter Sp ◽

Floating Treatment Wetlands

Diesel oil is considered a very hazardous fuel due to its adverse effect on the aquatic ecosystem, so its remediation has become the focus of much attention. Taking this into consideration, the current study was conducted to explore the synergistic applications of both plant and bacteria for cleaning up of diesel oil contaminated water. We examined that the application of floating treatment wetlands (FTWs) is an economical and superlative choice for the treatment of diesel oil contaminated water. In this study, a pilot scale floating treatment wetlands system having diesel oil contaminated water (1% w/v), was adopted using Cyperus laevigatus L and a mixture of hydrocarbons degrading bacterial strains; viz., Acinetobacter sp.61KJ620863, Bacillus megaterium 65 KF478214, and Acinetobacter sp.82 KF478231. It was observed that consortium of hydrocarbons degrading bacteria improved the remediation of diesel oil in combination with Cyperus laevigatus L. Moreover, the performance of the FTWs was enhanced by colonization of bacterial strains in the root and shoot of Cyperus laevigatus L. Independently, the bacterial consortium and Cyperus laevigatus L exhibited 37.46% and 56.57% reduction in diesel oil, respectively, while 73.48% reduction in hydrocarbons was exhibited by the joint application of both plant and bacteria in FTWs. Furthermore, microbial inoculation improved the fresh biomass (11.62%), dry biomass (33.33%), and height (18.05%) of plants. Fish toxicity assay evaluated the effectiveness of FTWs by showing the extent of improvement in the water quality to a level that became safe for living organisms. The study therefore concluded that Cyperus laevigatus L augmented with hydrocarbons degrading bacterial consortium exhibited a remarkable ability to decontaminate the diesel oil from water and could enhance the FTWs performance.

Download Full-text

Remediation of sewage and industrial effluent using bacterially assisted floating treatment wetlands vegetated with Typha domingensis

Water Science & Technology ◽

10.2166/wst.2016.405 ◽

2016 ◽

Vol 74 (9) ◽

pp. 2192-2201 ◽

Cited By ~ 35

Author(s):

Amna Ijaz ◽

Zafar Iqbal ◽

Muhammad Afzal

Keyword(s):

Environmental Quality ◽

Industrial Wastewater ◽

Industrial Effluent ◽

Oxygen Demand ◽

Sewage Water ◽

Quality Parameters ◽

Treatment Wetlands ◽

Polluted Water ◽

Typha Domingensis ◽

Floating Treatment Wetlands

This investigation reports the quantitative assessment of endophyte-assisted floating treatment wetlands (FTWs) for the remediation of sewage and industrial wastewater. Typha domingensis was used to vegetate FTWs that were subsequently inoculated with a consortium of pollutant-degrading and plant growth-promoting endophytic bacteria. T. domingensis, being an aquatic species, holds excellent potential to remediate polluted water. Nonetheless, investigation conducted on Madhuana drain carrying industrial and sewage water from Faisalabad City revealed the percentage reduction in chemical oxygen demand (COD) and biochemical oxygen demand (BOD5) to be 87% and 87.5%, respectively, within 96 h on coupling the plant species with a consortium of bacterial endophytes. With the endophytes surviving in plant tissue, maximal reduction was obtained in not only the aforementioned pollution parameters but for other major environmental quality parameters including nutrients (N and P), ions (Na+ and K+), Cl−, and SO42− as well, which showed percentage reductions up to 90%, 39%, 77%, 91.8%, 40%, and 60%, respectively. This significant improvement in polluted wastewater quality treated with the proposed method render it safe to be discharged freely in larger water bodies as per the National Environmental Quality Standards (NEQS) of Pakistan or to be reused safely for irrigation purposes; thus, FTWs provide a sustainable and affordable approach for in situ remediation of sewage and industrial wastewater.

Download Full-text

Bacterial Augmented Floating Treatment Wetlands for Efficient Treatment of Synthetic Textile Dye Wastewater

Sustainability ◽

10.3390/su12093731 ◽

2020 ◽

Vol 12 (9) ◽

pp. 3731 ◽

Cited By ~ 3

Author(s):

Neeha Nawaz ◽

Shafaqat Ali ◽

Ghulam Shabir ◽

Muhammad Rizwan ◽

Muhammad Bilal Shakoor ◽

...

Keyword(s):

Plant Growth ◽

Oxygen Demand ◽

Textile Wastewater ◽

Treatment Wetlands ◽

Textile Dye ◽

Bacterial Strains ◽

Bacterial Inoculation ◽

Removal Rates ◽

Efficient Treatment ◽

Floating Treatment Wetlands

Floating treatment wetland (FTW) is an innovative, cost effective and environmentally friendly option for wastewater treatment. The dyes in textile wastewater degrade water quality and pose harmful effects to living organisms. In this study, FTWs, vegetated with Phragmites australis and augmented with specific bacteria, were used to treat dye-enriched synthetic effluent. Three different types of textile wastewater were synthesized by adding three different dyes in tap water separately. The FTWs were augmented with three pollutants degrading and plant growth promoting bacterial strains (i.e., Acinetobacter junii strain NT-15, Rhodococcus sp. strain NT-39, and Pseudomonas indoloxydans strain NT-38). The water samples were analyzed for pH, electrical conductivity (EC), total dissolved solid (TDS), total suspended solids (TSS), chemical oxygen demand (COD), biological oxygen demand (BOD), color, bacterial survival and heavy metals (Cr, Ni, Mn, Zn, Pb and Fe). The results indicated that the FTWs removed pollutants and color from the treated water; however, the inoculated bacteria in combination with plants further enhanced the remediation potential of floating wetlands. In FTWs with P. australis and augmented with bacterial inoculum, pH, EC, TDS, TSS, COD, BOD and color of dyes were significantly reduced as compared to only vegetated and non-vegetated floating treatment wetlands without bacterial inoculation. Similarly, the FTWs application successfully removed the heavy metal from the treated dye-enriched wastewater, predominately by FTWs inoculated with bacterial strains. The bacterial augmented vegetated FTWs, in the case of dye 1, reduced the concentration of Cu, Ni, Zn, Fe, Mn and Pb by 75%, 73.3%, 86.9%, 75%, 70% and 76.7%, respectively. Similarly, the bacterial inoculation to plants in the case of dye 2 achieved 77.5% (Cu), 73.3% (Ni), 83.3% (Zn), 77.5% (Fe), 66.7% (Mn) and 73.3% (Pb) removal rates. Likewise in the case of dye 3, which was treated with plants and inoculated bacteria, the metals removal rates were 77.5%, 73.3%, 89.7%, 81.0%, 70% and 65.5% for Cu, Ni, Zn, Fe, Mn and Pb, respectively. The inoculated bacteria showed persistence in water, in roots and in shoots of the inoculated plants. The bacteria also reduced the dye-induced toxicity and promoted plant growth for all three dyes. The overall results suggested that FTW could be a promising technology for the treatment of dye-enriched textile effluent. Further research is needed in this regard before making it commercially applicable.

Download Full-text

TREATMENT OF TEXTILEWASTEWATER USING MICROBES’ INOCULATED FREE-FLOATINGAQUATICPLANTSBASED WETLANDS

TEXTEH Proceedings ◽

10.35530/tt.2019.26 ◽

2019 ◽

Vol 2019 ◽

pp. 114-124

Author(s):

Muhammad Qamar TUSIEF ◽

Mumtaz Hasan MALIK ◽

Muhammad MOHSIN ◽

Hafiz Naeem ASGHAR

Keyword(s):

Textile Industry ◽

Municipal Wastewater ◽

Oxygen Demand ◽

Textile Wastewater ◽

Pistia Stratiotes ◽

Physiochemical Parameters ◽

Degrading Bacteria ◽

Alkaline Side ◽

Plant Growth Promoting ◽

Floating Treatment Wetlands

Textile wastewater is a big source of aquatic and environmental pollution. Currently, various physicochemical textile effluent treatments are practiced in the textile industry, but they have challenges with respect to cost, maintenance, labour management, chemicals usage and production of additional sludge. The present study was carried out to develop a less expensive, chemical- free, green and sustainable plant based floating treatment wetlands (FTWs) system augmented with bacteria to remedy the effluents from textile finishing unit. Two free-floating aquatic plants, Eichhornia crassipes and Pistia stratiotes, were vegetated to develop FTWs system and its efficacy was studied with and without inoculating two plant growth-promoting and pollutant- degrading bacteria, Bacillus cereus and Bacillus subtilis. The worth of this system was analyzed by screening physicochemical parameters like potential hydrogen (pH), electric conductivity (EC), total dissolved solids (TDS), total suspended solids (TSS), biological oxygen demand (BOD) and chemical oxygen demand (COD) of wastewater for hydraulic retention periods of 0, 24, 48 and 72 hours. The pH of the treated wastewaters was changed from acidic to neutral/alkaline side while a significant reduction was found in all other physiochemical parameters as per set limits of industrial and municipal wastewater standards as specified by the National Environmental Quality Standards (NEQS) of Pak istan and Zero Discharge of Hazardous Chemicals (ZDHC) program.

Download Full-text

A novel three-stage bioreactor for the effective detoxification of sodium dodecyl sulphate from wastewater

Water Science & Technology ◽

10.2166/wst.2017.389 ◽

2017 ◽

Vol 76 (8) ◽

pp. 2167-2176 ◽

Cited By ~ 2

Author(s):

P. S. Ambily ◽

Sharrel Rebello ◽

K. Jayachandran ◽

M. S. Jisha

Keyword(s):

Pseudomonas Aeruginosa ◽

Sodium Dodecyl Sulphate ◽

Anionic Surfactants ◽

Low Cost ◽

Sodium Dodecyl ◽

Oxygen Demand ◽

Packed Bed ◽

Packed Bed Reactor ◽

Continuous Treatment ◽

After Effects

Anionic surfactants like sodium dodecyl sulphate (SDS), due to its extensive disposal to water bodies cause detrimental effects to the ecosystem. Among the various attempts to reduce the after effects of these toxicants, microbial induced bioremediation serves as a promising strategy. The current study aimed to develop a three stage bioreactor to remediate anionic surfactants in wastewater using effective bacterial isolates. Screening of effective SDS biodegraders led to isolation of Pseudomonas aeruginosa (MTCC 10311). Treatment of synthetic effluent with an immobilized packed bed reactor at a flow rate of 5 mL h−1 resulted in 81 ± 2% SDS eliminations and 70 ± 1% reduction in chemical oxygen demand (COD) in five cycles (6 h per cycle). The hydraulic retention time of the reactor was found to be 6 h. Combinatorial usage of a three stage bioreactor, involving aeration, adsorption with low cost scrap rubber granules and treatment with immobilized Pseudomonas aeruginosa, successfully reduced SDS concentrations and COD of wastewater to 99.8 ± 0.1% and 99 ± 1%, respectively, in 18 h by continuous treatment. Half-life of the three stage bioreactor was 72 h. In addition to reducing the surfactant concentrations, this novel bioreactor could resolve the surfactant associated foaming problems in treatment plants, which make it more unique.

Download Full-text

Identifikasi Daging Tikus Pada Produk Baso Dengan Metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

YARSI Medical Journal ◽

10.33476/jky.v26i2.394 ◽

2018 ◽

Vol 26 (2) ◽

pp. 058

Author(s):

Anna P. Roswiem ◽

Triayu Septiani

Keyword(s):

Sodium Dodecyl Sulphate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Sds Page

Bahan baku untuk membuat baso adalah daging hewan, pada umumnya dari daging sapi, ayam, ikan dan babi. Di beberapa daerah di Indonesia terjadi kasus baso tikus. Tujuan penelitian ini adalah menguji ada tidaknya kandungan daging tikus pada produk baso yang dijual di pasar Cempaka Putih-Kecamatan Kramat Jakarta Pusat dan di pedagang baso atau mie baso di sekitar kampus Universitas YARSI Jakarta. Daging adalah protein salah satu metode untuk mengidentifikasi protein adalah metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Hasil penelitian menunjukkan bahwa dari 6 sampel baso terindikasi ada 2 sampel baso dengan nomor 1 dan 5 yang dibuat dari campuran daging sapi dan tikus; ada 1 sampel baso dengan nomor 6 yang terbuat dari daging tikus; dan 2 sampel baso dengan nomor 2 dan 3 yang terbuat dari campuran sapi dan babi, dan hanya 1 sampel baso dengan nomor sampel 4 yang benar-benar terbuat dari daging sapi.

Download Full-text