The Parental Pesticide and Offspring’s Epigenome Study: Towards an Integrated Use of Human Biomonitoring of Exposure and Effect Biomarkers

In Morocco, due to the lack of education and the presence of a counterfeit market, pesticides constitute a major problem to be addressed by occupational and environmental health agencies. This paper aims to introduce the PaPOE (Parental Pesticides and Offspring Epigenome) prospective study and its goals, to motivate the study rationale and design, and to examine comprehensively whether multi-residue exposure to commonly used pesticides could induce epigenetic alterations through the oxidative stress pathway. The PaPOE project includes a cross-sectional study assessing the occupational exposure among 300 farmworkers in Meknes, and initiates a birth cohort of 1000 pregnant women. Data and biological samples are collected among farmworkers, and throughout pregnancy, and at birth. Oxidative stress biomarkers include Glutathione, Malondialdehyde, and 8-OHdG. Global and gene-specific DNA methylation is assessed. The study began enrollment in 2019 and is ongoing. As of 30 June 2021, 300 farmworkers and 125 pregnant women have enrolled. The results are expected to showcase the importance of biomonitoring for understanding individual risks, and to identify a number of regions where DNA methylation status is altered in the pesticides-exposed population, paving the way for an integrated biomonitoring system in Morocco and Africa to assess environmental exposures and their long-term health consequences.

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Characterization of Differentially Expressed Circulating miRNAs in Metabolically Healthy versus Unhealthy Obesity

Biomedicines ◽

10.3390/biomedicines9030321 ◽

2021 ◽

Vol 9 (3) ◽

pp. 321

Author(s):

Susana Rovira-Llopis ◽

Rubén Díaz-Rúa ◽

Carmen Grau-del Valle ◽

Francesca Iannantuoni ◽

Zaida Abad-Jimenez ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Metabolism ◽

Insulin Signalling ◽

Cross Sectional Study ◽

Differentially Expressed ◽

Circulating Mirnas ◽

Oxidative Stress Biomarkers ◽

Cross Sectional ◽

Metabolically Healthy Obese ◽

Metabolically Healthy

Obese individuals without metabolic comorbidities are categorized as metabolically healthy obese (MHO). MicroRNAs (miRNAs) may be implicated in MHO. This cross-sectional study explores the link between circulating miRNAs and the main components of metabolic syndrome (MetS) in the context of obesity. We also examine oxidative stress biomarkers in MHO vs. metabolically unhealthy obesity (MUO). We analysed 3536 serum miRNAs in 20 middle-aged obese individuals: 10 MHO and 10 MUO. A total of 159 miRNAs were differentially expressed, of which, 72 miRNAs (45.2%) were higher and 87 miRNAs (54.7%) were lower in the MUO group. In addition, miRNAs related to insulin signalling and lipid metabolism pathways were upregulated in the MUO group. Among these miRNAs, hsa-miR-6796-5p and hsa-miR-4697-3p, which regulate oxidative stress, showed significant correlations with glucose, triglycerides, HbA1c and HDLc. Our results provide evidence of a pattern of differentially expressed miRNAs in obesity according to MetS, and identify those related to insulin resistance and lipid metabolism pathways.

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Urinary 8-Hydroxy-2′-deoxyguanosine (8-OHdG) Concentrations and Menstrual Cycle Characteristics in Female University Students

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph15122616 ◽

2018 ◽

Vol 15 (12) ◽

pp. 2616

Author(s):

Shoko Konishi ◽

Jun Yoshinaga ◽

Yukiko Nishihama ◽

Yu Onoda ◽

Youichi Chisaki ◽

...

Keyword(s):

Oxidative Stress ◽

Menstrual Cycle ◽

University Students ◽

Polycystic Ovary ◽

Cross Sectional Study ◽

Menstrual Pain ◽

Oxidative Stress Biomarkers ◽

Cross Sectional ◽

Menstrual Cycles ◽

The Mean

Higher concentrations of oxidative stress biomarkers are found in women with polycystic ovary syndrome (PCOS) and endometriosis, conditions linked to irregular menstrual cycles and menstrual pain. The aim of the present study was to test whether women with higher oxidative stress are more likely to show irregular menstrual cycles and severe menstrual pain compared with women with lower oxidative stress. A cross-sectional study was conducted targeting female university students with a mean (SD) age of 20.5 (1.8) years (n = 188). Participants completed a questionnaire on reproductive characteristics and anthropometry and kept a menstrual cycle diary for 5 consecutive months. Urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), cotinine, and creatinine concentrations were measured once during the study period. The mean (SD) value of the urinary 8-OHdG concentration was 4.7 (2.0) μg/g of creatinine. A total of 1021 menstrual cycles were recorded. The participants were categorized as either having regular (68%) or irregular (18%) cycles or oligomenorrhea (13%) or polymenorrhea (1%). The urinary 8-OHdG concentration did not significantly differ across menstrual cycle regularity or pain categories. Even after adjusting for age, body mass index (BMI), and urinary cotinine concentrations, having irregular cycles or more severe menstrual pain was not associated with urinary 8-OHdG concentration.

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Nitric oxide products are not associated with metabolic syndrome

Journal of Medical Biochemistry ◽

10.2478/jomb-2018-0035 ◽

2019 ◽

Vol 38 (3) ◽

pp. 361-367 ◽

Cited By ~ 2

Author(s):

Aleksandra Klisic ◽

Gordana Kocic ◽

Nebojsa Kavaric ◽

Radmila Pavlovic ◽

Ivan Soldatovic ◽

...

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Metabolic Syndrome ◽

Multivariate Logistic Regression Analysis ◽

International Diabetes Federation ◽

Cross Sectional Study ◽

Oxidative Stress Biomarkers ◽

Anthropometric Parameters ◽

Cross Sectional ◽

Stress Biomarkers

SummaryBackgroundNitric oxide (NO) is oxidative stress biomarker which is regarded as one of the key determinants of energy metabolism and vascular tone. Considering the controversial reports on the association between nitric oxide products (NOx) and metabolic syndrome (MetS), the aim of the current study was to examine that potential relationship. Additionally, we aimed to evaluate a broad spectrum of other oxidative stress biomarkers [i.e., malondialdehyde (MDA), advanced oxidation protein products (AOPP), xanthine oxidoreductase (XOD), xanthine oxidase (XO) xanthine dehydrogenase (XDH)] in relation with MetS.MethodsA total of 109 volunteers (46.8% of them with MetS) were included in this cross-sectional study. Biohemical and anthropometric parameters, as well as blood pressure, were obtained. The MetS was diagnosed according to the International Diabetes Federation criteria.ResultsMultivariate logistic regression analysis showed that XOD (OR=1.011; 95% CI 1.002–1.019; p=0.016), XO (OR=1.014; 95% CI 1.003–1.026; p=0.016), MDA (OR=1.113; 95% CI 1.038–1.192; p=0.003) and AOPP (OR=1.022; 95% CI 1.005–1.039; p=0.012) were the independent predictors of MetS, whereas no association between NOx and MetS was found. As XOD rose for 1 U/L, XO for 1 U/L, MDA for 1 μmol/L and AOPP for 1 T/L, probability for MetS rose for 1.1%, 1.4%, 11.3% and 2.2%, respectively. Adjusted R2for the Model was 0.531, which means that 53.1% of variation in MetS could be explained with this Model.ConclusionUnlike XOD, MDA and AOPP, NOx is not associated with MetS.

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DNA Methylation Status of the Methylenetetrahydrofolate Reductase Gene is associated with Depressive Symptoms in Japanese Workers: A Cross-Sectional Study

Journal of Neurology and Neurological Disorders ◽

10.15744/2454-4981.2.304 ◽

2015 ◽

Vol 2 (3) ◽

Author(s):

Miyaki K ◽

Song Y ◽

Suzuki T ◽

Eguchi H ◽

Kawakami N ◽

...

Keyword(s):

Dna Methylation ◽

Depressive Symptoms ◽

Methylenetetrahydrofolate Reductase ◽

Methylation Status ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Japanese Workers ◽

Reductase Gene ◽

Methylenetetrahydrofolate Reductase Gene

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Dietary total antioxidant capacity is inversely associated with depression, anxiety and some oxidative stress biomarkers in postmenopausal women: a cross-sectional study

Annals of General Psychiatry ◽

10.1186/s12991-019-0225-7 ◽

2019 ◽

Vol 18 (1) ◽

Cited By ~ 15

Author(s):

Maryam Abshirini ◽

Fereydoun Siassi ◽

Fariba Koohdani ◽

Mostafa Qorbani ◽

Hadis Mozaffari ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Cross Sectional Study ◽

Sectional Study ◽

Oxidative Stress Biomarkers ◽

Cross Sectional ◽

Stress Biomarkers ◽

Total Antioxidant ◽

Dietary Total Antioxidant Capacity

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Serum cortisol but not oxidative stress biomarkers are related to frailty: results of a cross-sectional study in Spanish older adults

Journal of Toxicology and Environmental Health Part A ◽

10.1080/15287394.2019.1654639 ◽

2019 ◽

Vol 82 (14) ◽

pp. 815-825 ◽

Cited By ~ 7

Author(s):

Diego Marcos-Pérez ◽

María Sánchez-Flores ◽

Ana Maseda ◽

Laura Lorenzo-López ◽

José C. Millán-Calenti ◽

...

Keyword(s):

Oxidative Stress ◽

Older Adults ◽

Serum Cortisol ◽

Cross Sectional Study ◽

Sectional Study ◽

Oxidative Stress Biomarkers ◽

Cross Sectional ◽

Stress Biomarkers

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Evaluation of Oxidative Stress and Biochemical Biomarkers, and Psychological Parameters in Cement Plant Workers

10.21203/rs.3.rs-121007/v1 ◽

2020 ◽

Author(s):

Faezeh Darabi ◽

Majid Keshavarzi ◽

Media Noori Abdullah ◽

Fatemeh Dehghani ◽

Saeed Yousefinejad ◽

...

Keyword(s):

Oxidative Stress ◽

Cross Sectional Study ◽

Cement Industry ◽

Control Group ◽

Cement Plant ◽

Oxidative Stress Biomarkers ◽

Cross Sectional ◽

Hazardous Chemical ◽

Wide Range ◽

Psychological Parameters

Abstract BackgroundThe cement industry is one of the main world industries with exposure to a wide range of hazardous chemical and physical occupational agents that can increase free radicals and cause diseases. The aim of this study was to evaluate oxidative stress, and biochemical markers and psychological parameters among cement plant workers who were exposed to variety of hazardous occupational agents.MethodsIn this cross-sectional study 40 workers exposed to cement and 40 office employees, were selected as the exposed and control group, respectively. Exposure to cement dust, silica and noise were respectively assessed using the NIOSH 0600, NIOSH 7601 and noise dosimetry methods. Oxidative stress biomarkers including malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC), and biochemical parameters were measured in the serum of all participants. Depression, anxiety and stress were assessed by Depression Anxiety Stress Scales (DASS-21) questioner. Statistical analysis was performed by SPSS22 software. Independent t-test, Mann-Whitney U, Chi-square were used to evaluate the relation between variables. ResultsThe results demonstrated that the level of MDA as a marker of oxidative stress was significantly higher in the exposed group.Antioxidant enzymes including SOD and CAT were significantly higher, and TAC was insignificantly lower in the exposed group. Alkaline phosphatase (ALP), aspartate transaminase (AST), depression and stress were also significantly higher in the exposure group. It seems like working in cement plants, can have hazardous effects on liver function, enzyme activity and mental health.

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