scholarly journals Determination of Cytisine and N-Methylcytisine from Selected Plant Extracts by High-Performance Liquid Chromatography and Comparison of Their Cytotoxic Activity

Toxins ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 557
Author(s):  
Anna Petruczynik ◽  
Karol Wróblewski ◽  
Justyna Misiurek ◽  
Tomasz Plech ◽  
Karolina Szalast ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Plant Extracts ◽  
High Performance ◽  
Cancer Cell Lines ◽  
Breast Adenocarcinoma

Quinolizidine alkaloids exhibit various forms of biological activity. A lot of them were found in the Leguminosae family, including Laburnum and Genista. The aim of the study was the optimization of a chromatographic system for the analysis of cytisine and N-methylcytisine in various plant extracts as well as an investigation of the cytotoxic activities of selected alkaloids and plant extracts obtained from Laburnum anagyroides, Laburnum anagyroides L. quercifolium, Laburnum alpinum, Laburnum watereri, Genista germanica, and Genista tinctoria against various cancer cell lines. The determination of investigated compounds was performed by High Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD), while High Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight–Mass Spectrometry (HPLC-QTOF-MS) was applied for the qualitative analysis of plant extracts. The retention, separation selectivity, peaks shape, and systems efficiency obtained for cytisine and N-methylcytisine in different chromatographic systems were compared. The application of columns with alkylbonded and phenyl stationary phases led to a very weak retention of cytisine and N-methylcytisine, even when the mobile phases containing only 5% of organic modifiers were used. The strongest retention was observed when hydrophilic interaction chromatography (HILIC) or especially when ion exchange chromatography (IEC) were applied. The most optimal system in terms of alkaloid retention, peak shape, and system efficiency containing an strong cation exchange (SCX) stationary phase and a mobile phase consisted of 25% acetonitrile and formic buffer at pH 4.0 was applied for investigating alkaloids analysis in plant extracts. Cytotoxic properties of the investigated plant extracts as well as cytisine and N-methylcytisine were examined using human tongue squamous carcinoma cells (SCC-25), human pharyngeal squamous carcinoma cells (FaDu), human triple-negative breast adenocarcinoma cell line (MDA-MB-231), and human breast adenocarcinoma cell line (MCF-7). The highest cytotoxic activity against FaDu, MCF-7, and MDA-MB cancer cell lines was observed after applying the Genista germanica leaves extract. In contrast, the highest cytotoxic activity against SCC-25 cell line was obtained after treating with the seed extract of Laburnum watereri. The investigated plant extracts exhibit significant cytotoxicity against the tested human cancer cell lines and seem to be promising for further research on its anticancer activity.

scholarly journals PRODUCTION OF CAMPTOTHECIN FROM ENDOPHYTIC FUNGI AND CHARACTERIZATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ANTICANCER ACTIVITY AGAINST COLON CANCER CELL LINE.

2018 ◽  
Vol 11 (3) ◽  
pp. 166 ◽  
Author(s):  
Aswini A ◽  
Soundhari C
Keyword(s):  
Colon Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cell Line ◽  
Cancer Cell ◽  
High Performance ◽  
Cancer Cell Line ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Biologically Active

 Objective: Scientists are showing increasing interest in studying fungal endophytes as sources of novel and biologically active compounds. The present study was attempted to isolate and characterize camptothecin (CPT) from endophytic fungus Aspergillus niger isolated from Indian Piper betel plant and detection of its anticancer potential on colon cancer cell line.Methods: The production of CPT was confirmed by high-performance liquid chromatography techniques. The effect of CPT on hematopoietic cell transplantation (HCT) colon cancer cell line was studied by MTT assay.Results: The amount of CPT which is isolated from A. niger was found to be around 0.175 mg/L. Significant death of HCT cells was observed and was non-toxic to normal vero cell line.Conclusion: Hence, CPT can be used as a potential lead compound in cancer research.

scholarly journals Cytotoxic Activity of Two Cembranoid Diterpenes from Nicotiana Sylvestris Against Three Human Cancer Cell Lines

2017 ◽  
Vol 5 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Amir Reza Jassbi ◽  
Marzieh Vafapour ◽  
Ardeshir Shokrollahi ◽  
Omidreza Firuzi ◽  
Mehdi Zare ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Lymphoblastic Leukemia ◽  
Nicotiana Sylvestris ◽  
Cancer Cell Lines ◽  
Breast Adenocarcinoma ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines

Background: Two cembranoid diterpenes, [(1S, 2E, 4R, 6R, 7E, 11E)-2, 7, 11-cembratriene-4, 6-diol] (1) and its epimer [(1S, 2E, 4S, 6R,7E,11E)-2, 7, 11-cembratriene-4, 6-diol] (2) were isolated from surface dichloromethane washings and chloroform extract of Nicotiana sylvestris leaves. Methods: The compounds were purified using silica gel column- thin layer- and flash column chromatography methods. The structures of the isolated compounds were elucidated using spectroscopic analysis and their 1H and 13C NMR spectroscopic data were compared with those of authentic samples reported in the literature. The cytotoxic activity of 1 and 2 against three human cancer cell lines, including LS180 (human colon adenocarcinoma), MCF-7 (human breast adenocarcinoma) and MOLT-4 (human lymphoblastic leukemia) were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric bioassay. Results: The IC50 values of compounds 1 and 2 were calculated to be between (28.4±3.7 up to 44.0±6.4 µM) (mean±S.E.M.) for the above mentioned cell lines.

Cytotoxic activity of plant extracts from Brazilian biome in prostate cancer cell lines

2017 ◽  
Author(s):  
Sarah Cardoso ◽  
Matheus Andrade ◽  
Vinicius Cunha ◽  
Cristian Silva ◽  
Patricia Rodrigues ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Plant Extracts ◽  
Prostate Cancer Cell ◽  
Cancer Cell Lines ◽  
Prostate Cancer Cell Lines

scholarly journals Determination of Selected Isoquinoline Alkaloids from Mahonia aquifolia; Meconopsis cambrica; Corydalis lutea; Dicentra spectabilis; Fumaria officinalis; Macleaya cordata Extracts by HPLC-DAD and Comparison of Their Cytotoxic Activity

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 575 ◽  
Author(s):  
Anna Petruczynik ◽  
Tomasz Plech ◽  
Tomasz Tuzimski ◽  
Justyna Misiurek ◽  
Barbara Kaproń ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Plant Extracts ◽  
Squamous Carcinoma ◽  
Breast Adenocarcinoma ◽  
Squamous Carcinoma Cells ◽  
Macleaya Cordata ◽  
Fumaria Officinalis ◽  
Mcf 7

Alkaloids have protective functions for plants and can play an important role in living organisms. Alkaloids may have a wide range of pharmacological activities. Many of them have cytotoxic activity. Nowadays, cancer has become a serious public health problem. Searching for effective drugs with anticancer activity is one of the most significant challenges of modern scientific research. The aim of this study was the investigation of cytotoxic activity of extracts obtained from Corydalis lutea root and herb, Dicentra spectabilis root and herb, Fumaria officinalis, Macleaya cordata leaves and herb, Mahonia aquifolia leaves and cortex, Meconopsis cambrica root and herb on FaDu, SCC-25, MCF-7, and MDA-MB-231 cancer cell lines. The cytotoxic activity of these extracts has not been previously tested for these cell lines. The aim was also to quantify selected alkaloids in the investigated extracts by High Performance Liquid Chromatography (HPLC). The analyses of alkaloid content were performed using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water and ionic liquid (IL). Cytotoxic effect of the tested plant extracts and respective alkaloid standards were examined using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), human triple-negative breast adenocarcinoma cell line (MDA-MB-231). All investigated plant extracts possess cytotoxic activity against tested cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu, SCC-25, and MCF-7 cell lines was estimated for Macleaya cordata leaf extract, while the highest cytotoxic activity against MDA-MB-231 cell line was obtained for Macleaya cordata herb extract. Differences in cytotoxic activity were observed for extracts obtained from various parts of investigated plants. In almost all cases the cytotoxic activity of investigated plant extracts, especially at the highest concentration against tested cell lines was significantly higher than the activity of anticancer drug etoposide. Our investigations exhibit that these plant extracts can be recommended for further in vivo experiments to confirm their anticancer activity.

scholarly journals Anatolicin, a Highly Potent and Selective Cytotoxic Sesquiterpene Coumarin from the Root Extract of Heptaptera anatolica

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1153 ◽  
Author(s):  
Fatma Tosun ◽  
John Beutler ◽  
Tanya Ransom ◽  
Mahmut Miski
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Kidney Cancer ◽  
Spectral Properties ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Root Extract ◽  
Selective Cytotoxicity

Seven known sesquiterpene coumarins and a new sesquiterpene coumarin, anatolicin (8), were isolated from the dichloromethane extract of the roots of Heptaptera anatolica. Structures of these compounds were elucidated based on their spectral properties. While some of these sesquiterpene coumarins showed modest cytotoxic activity against COLO205, KM12, A498, UO31, and TC32 cancer cell lines, selective cytotoxicity of anatolicin (8) and 14′-acetoxybadrakemin (7) were observed at nanomolar level against the UO31 kidney cancer cell line.

scholarly journals Cytotoxicity Profile of Calix[4]pyrrole Derivatives on HeLa and MCF-7 Human Cancer Cell Lines via In vitro Study and Molecular Modelling

2021 ◽  
Vol 12 (5) ◽  
pp. 6991-7000
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Quinone Reductase ◽  
Breast Adenocarcinoma ◽  
Molecular Docking Study ◽  
Mcf 7

Amongst significant macromolecules, the cytotoxic activity of calixarene analogs is significant due to their unique biomedical properties. Recently, calix[4]pyrrole have shown remarkable efficacy and have extended new dimensions towards different biomedical and therapeutic applications. Herein, meso-tetra (methyl) meso-tetra (3-methoxy 4-hydroxy phenyl) calix[4]pyrrole (HMCP) have been studied for their remarkable cytotoxic activity against HeLa and human breast adenocarcinoma (MCF-7) cancer cell lines in comparison to its hydrazide derivative, meso-tetra (methyl) meso-tetra (3-methoxy 4-hydroxy phenoxy acetatohydrazide) calix[4]pyrrole (MCPTH). Cytotoxicity assays were studied at varying concentrations where HMCP molecule was very active against cancer cell lines with G150 values less than 10. The cell viability has been examined by SRB assay. The anti-cancerous activity results of HMCP can be potentially extended with applications in cancer therapies. Furthermore, the structure and anti-cancer activity relationship has been supported by molecular docking study of the active compounds against quinone reductase-2 (PDB ID 4ZVM) protein.

scholarly journals Enzymatically Synthesized Ginsenoside Exhibits Antiproliferative Activity in Various Cancer Cell Lines

2019 ◽  
Vol 9 (5) ◽  
pp. 893 ◽  
Author(s):  
Sumangala Darsandhari ◽  
Biplav Shrestha ◽  
Ramesh Pandey ◽  
Sanghun Lee ◽  
Hye Jung ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Cell Lines ◽  
Cancer Cell ◽  
High Performance ◽  
Cancer Cell Lines ◽  
Skin Melanoma ◽  
Compound K ◽  
Melanoma B16f10 ◽  
Pharmacologically Active

A glycoside derivative of compound K (CK) was synthesized by using a glycosyltransferase, and its biological activity was tested against various cancer-cell lines. A regiospecific, β-1,4-galactosyltransferase (LgtB) converted 100% of 0.5 mmol CK into a galactosylated product in 3 h. The structure of the synthesized derivative was revealed with high performance liquid chromatography, mass spectroscopy, as well as nuclear magnetic resonance analyses, and it was recognized as 20-O-β-D-lactopyranosyl-20(S)-protopanaxadiol (CKGal). Out of the four cancer-cell lines tested (gastric carcinoma (AGS), skin melanoma (B16F10), cervical carcinoma (HeLa), and brain carcinoma (U87MG)), CKGal showed the best cytotoxic ability against B16F10 and AGS when compared to other ginsenosides like compound K (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol), Rh2 (3-O-β-D-glucopyranosyl-20(S)-protopanaxadiol), and F12 (3-O-β-D-glucopyranosyl-12-O-β-D-glucopyranosyl-20(S)-protopanaxadiol). Thus, the synthesized derivative (CKGal) is a pharmacologically active ginsenoside.

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