scholarly journals Morphological Analysis Reveals a Compartmentalized Duct in the Venom Apparatus of the Wasp Spider (Argiope bruennichi)

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 270
Author(s):  
Henrike Schmidtberg ◽  
Björn M. von Reumont ◽  
Sarah Lemke ◽  
Andreas Vilcinskas ◽  
Tim Lüddecke

Spiders are one of the most successful groups of venomous animals, but surprisingly few species have been examined in sufficient detail to determine the structure of their venom systems. To learn more about the venom system of the family Araneidae (orb-weavers), we selected the wasp spider (Argiope bruennichi) and examined the general structure and morphology of the venom apparatus by light microscopy. This revealed morphological features broadly similar to those reported in the small number of other spiders subject to similar investigations. However, detailed evaluation of the venom duct revealed the presence of four structurally distinct compartments. We propose that these subunits facilitate the expression and secretion of venom components, as previously reported for similar substructures in pit vipers and cone snails.

2021 ◽  
Vol 63 (9) ◽  
pp. 19-24
Author(s):  
Hong Quang Bui ◽  
◽  

This paper uses scanning electron microscopy (SEM) analyses and light microscopy (LM) to study pollen grains of species of the family Oleaceae Hoffmanns. & Link distributed in Vietnam, including 23 species belonging to 6 genera of the family (Oleaceae). Pollen grains of all species (Oleaceae) in Vietnam are isopolar, radial, 3- prolate pollen grains small size, with two types: subprolate type (Jasminum and Olea) and prolate spheroidal type (Jasminum, Fraxinus, Ligustrum, Osmanthus, and Chengiodendron). The surface of the pollen grains in most species is reticulate. These are important morphological features to classify the Nhai family through pollen morphology


Author(s):  
E. S. Boatman ◽  
G. E. Kenny

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.


2009 ◽  
Vol 18 (1) ◽  
pp. 11-16
Author(s):  
E.V. Soldatenko ◽  
A.A. Petrov

The morphology of the copulatory apparatus and associated cuticular structures in Planorbis planorbis was studied by light microscopy, SEM, TEM and confocal laser scanning microscopy. The significance of these cuticular structures for the taxonomic status of the species and for the systematics of the family Planorbidae in general is discussed.


2021 ◽  
Vol 123 (6) ◽  
pp. 151761
Author(s):  
Tasuku Hiroshige ◽  
Kei-Ichiro Uemura ◽  
Shingo Hirashima ◽  
Kiyosato Hino ◽  
Akinobu Togo ◽  
...  

2018 ◽  
Vol 46 (1) ◽  
pp. 82-89
Author(s):  
Yanfen LU ◽  
Suxiao HAO ◽  
Hui GENG ◽  
Yuncong YAO

Flavonoids are important secondary metabolites, whose content and composition such as anthocyanin and flavonol have been associated with fruit quality and affect fruit coloration. Crabapple (Malus spp.), with rich color and nutritive value, belongs to the family M. genus of the Rosaceae family, and it is widely used in landscape for its rich flavonoid compositions. Radiation mutagenesis breeding may increase the gene mutation frequency and enhance diversity of species, so it is a predominant approach for plant germplasm innovation. However, the changes of crabapple morphological characteristics and flavonoid compositions by radiation are not clear. In this study, we employed 60Coγ radiation to M.cv. ‘Royalty’ crabapple, and then surveyed the changes of leaves and flowers in the growth and morphology traits, color parameters, flavonoid composition, and the genetic diversity. The result found that 60Coγ radiation decreased plant height, stem diameter and leaf and flower areas. And it promoted the flavonoids accumulation in leaves, but inhibited that in flowers. Additionally, 60Coγ radiation improved DNA diversity. W60-7, W40-2 and W40-5 plants showed clear phenotypic variation by AFLP analysis. These results provide evidence that mutagenesis breeding may change the morphological features and flavonoid compositions, and expand crabapple germplasm resources and improve its application value. 


2020 ◽  
Vol 175 ◽  
pp. 15007 ◽  
Author(s):  
Vladimir Meshkov ◽  
Natalia Kochkovaya ◽  
Irina Usova

This work combines two approaches to the definition of the author’s style: statistical and linguistic (method of morphological analysis). The average data of the authors obtained on the basis of statisticalmorphological analysis are taken as an indications vector. The article shows that this technique allows to recognize the author’s style of the work with sufficient accuracy, on an average about 88 percent. It is shown that the closest measure of proximity to this technique is the Euclid distance. The prospects for the development of this approach areoutlined.


2019 ◽  
Vol 8 (4) ◽  
pp. 116-122
Author(s):  
Anna Andreevna Malyutina ◽  
Aleksandr Nikolaevich Vashanov ◽  
Mariya Ivanovna Tkacheva ◽  
Evgenia Sergeevna Tkach

The paper presents the results of a techno-morphological analysis of items made of antler obtained as a result of the collections from the 1960s-1990s from the site near the village of Michnievičy Smorgon District of the Grodno Region (north-western Belarus). Currently, more than 100 artifacts are known from this site, as well as a large number of fauna residues with no visible traces of processing. Radiocarbon dating was obtained for some categories of products, which link them to 9-2 thousand BC. The largest part of the collection refers to the period of the Mesolithic - Neolithic. At the first stage of work, the most expressive and numerous group of artifacts made of horn (24 exemplars), stored in the fonds of the Institute of History of the National Academy of Sciences of Belarus, was selected for a techno-morphological analysis. The degree of preservation of the external surface of objects is relatively good, which made it possible to analyze macro-traces related to the technology of manufacturing various categories of products, on the basis of which a process flow was proposed - from the selection of raw materials to the finished product. The analysis of the technological traces recorded on the products allowed us to highlight the differences in the manufacturing processes of the oldest tools. In addition, on the basis of the macro signs of utilitarian wear, preliminary observations on the functional using of objects were obtained. According to technological and morphological features, the whole of the analyzed material was divided into conditional categories of instruments with a selected heel and without it. The presence or absence of this element, apparently, influenced the method of using objects in various household situations.


Botany ◽  
2020 ◽  
Vol 98 (4) ◽  
pp. 197-208
Author(s):  
Letícia Silva Souto ◽  
Denise Maria Trombert Oliveira

Malpighiaceae have highly varied fruits, and their characteristics are traditionally used in taxa delimitation. However, fruit characters are homoplastic; therefore, detailed analyses are indispensable. Here, we have described winged-fruit ontogeny and anatomy of four Malpighiaceae species from different clades, and compared them with others described in the literature, with the goal of recognizing common developmental and structural patterns. Flowers and fruits were collected and processed for light microscopy. In the ovary, the main patterns were a uniseriate outer epidermis, a mesophyll with two or three distinct regions, and an inner epidermis formed by elongated or isodiametric cells. In the pericarp, the uniseriate exocarp appeared to be consistent throughout the family, and the outermost parenchymatic layers of the mesocarp and the inner layers formed by thickened-lignified cells prevailed. Some species also had a middle layer in the mesocarp, composed of lignified cells. The most substantial variation was in the endocarp and was independent of fruit type and phylogenetic position. Three structural patterns were recognized: sclerenchymatous cells, elongated and non-lignified cells, and a seed cushion. These data allow for the identification of important structural patterns in Malpighiaceae, and the high structural variation in the endocarp is promising for its systematics.


Author(s):  
Douglas C. Walker

In the evolution of morphological studies, morphological features and markedness have come to play increasingly important rôles. Feature notation in morphology allows the development of the notion of natural morphological class, renders explicit much of the internal structure of paradigms and permits the exploitation, suitably adapted, of many of the results obtained in phonology. Markedness considerations, particularly when linked to general theories of morphology, again highlight morphological structure, constrain the variety of permitted analyses, and indicate, at least in part, the expected direction of historical change. In the sections to follow, I will present an analysis of Old French nominal and adjectival inflection which makes key use of morphological features and the differences between the marked and unmarked values of these features. This study will be particularly concerned with constraining the morphological analysis of Old French and conversely, with using Old French data to investigate more general properties of morphological systems.


2014 ◽  
Vol 21 (1) ◽  
pp. 13-17
Author(s):  
Tulika Talukdar ◽  
Sobhan Kumar Mukherjee

The genus Senecio L. is one of the largest genera of flowering plants and is an important member of the tribe Senecioneae (Asteraceae). Phenotypic information, including a broad range of morphological characters is very crucial for phylogenetic reconsideration of any family, tribe or genus. In the family Asteraceae, very little attention has been paid to cypselar diversity, though it is regarded as taxonomically valuable. A sincere attempt has been made to study detailed cypselas macro and micro-morphological features of four species of Senecio L. These diacritical features could be used to strengthen current inter-specific concept of Senecio L.DOI: http://dx.doi.org/10.3329/bjpt.v21i1.19251Bangladesh J. Plant Taxon. 21(1): 13-17, 2014 (June)


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