scholarly journals Production of Microalgal Biomass Using Aquaculture Wastewater as Growth Medium

Water ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 106 ◽  
Author(s):  
Małgorzata Hawrot-Paw ◽  
Adam Koniuszy ◽  
Małgorzata Gałczyńska ◽  
Grzegorz Zając ◽  
Joanna Szyszlak-Bargłowicz
Keyword(s):  
Wastewater Treatment ◽  
Environmental Pollution ◽  
Lipid Content ◽  
Biomass Concentration ◽  
Huge Amount ◽  
Initial Value ◽  
Nitrogen And Phosphorus ◽  
Microalgal Biomass ◽  
High Lipid ◽  
Aquaculture Wastewater

Aquaculture wastewater contains a huge amount of substances that can cause environmental pollution. However, microalgae can absorb these compounds and convert them into useful biomass. In this study, Chlorella minutissima was grown in the wastewater resulting from saline aquaculture. The microalgae were found to effectively utilize nitrogen and phosphorus in the wastewater for its growth. During wastewater treatment, the cell density increased almost fivefold compared to the initial value (OD680 0.502). Moreover, batch culture resulted in the maximum biomass concentration and productivity of 4.77 g/L and 0.55 g/L/day, respectively. The contents of total nitrogen and total phosphorus in wastewater decreased by 88% and over 99%, respectively. In addition, the content of N-NO3 was reduced by 88.6%, N-NO2 by 74.3%, and dissolved orthophosphates (V) by 99%. At the beginning and throughout the experiment, the content of N-NH4 in wastewater remained below 0.05 mg/L. Furthermore, a high lipid content of 46.4% (w/w) was also obtained from the studied microalgae.

Optimization of lipid accumulation in Pleurastrum insigne for biodiesel production

2021 ◽  
Vol 16 (10) ◽  
pp. 144-155
Author(s):  
Van Lal Michael Chhandama ◽  
Belur Kumudini Satyan
Keyword(s):  
Lipid Content ◽  
Lipid Production ◽  
Statistical Design ◽  
High Growth ◽  
High Growth Rate ◽  
Biodiesel Production ◽  
Nitrogen And Phosphorus ◽  
Biodiesel Standards ◽  
High Lipid ◽  
Different Sources

Microalgae emerged as a competent feedstock for biodiesel production because of high growth rate and lipid content. This work focuses on isolation of novel microalgal strain from different sources of water for the production of biodiesel. The isolated microalgae, Pleurastrum insigne possessed high lipid content (~28 % dcw), further optimized to 57.06 % dcw using a statistical design (CCD) under Response Surface Methodology. Lipid production was optimized by nutrient (nitrogen and phosphorus) and pH stress. The different type of fatty acids present in the optimized lipid was also profiled using GCMS. Biodiesel yield was found to be 82.14 % of the total lipid and the fuel properties tested have met IS, ASTM and EN biodiesel standards.

scholarly journals Characterization and identification of freshwater microalgal strains toward biofuel production

BioResources ◽  
2011 ◽  
Vol 7 (1) ◽  
pp. 686-695
Author(s):  
Xun Yang ◽  
Pinghuai Liu ◽  
Zongdi Hao ◽  
Jie Shi ◽  
Sen Zhang
Keyword(s):  
Oleic Acid ◽  
Lipid Content ◽  
Biomass Concentration ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Biofuel Production ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Chlorella Sp ◽  
High Lipid

Fifty-three algal cultures were isolated from freshwater lakes in Hainan, China. Four microalgal isolates were selected because they could be successfully cultivated at high density and demostrated a strong fluorescence after being stained with nile red. These cultures were identified as strains of Chlorella sp. C11, Chlamydomonas reinhardtii C22, Monoraphidium dybowskii C29, and Chlorella sp. HK12 through microscopic and 18S rDNA analysis. Under similar conditions, the lipid productivity of Chlorella sp. C11, Chla. reinhardtii C22, M. dybowskii C29 , and Chlorella sp. HK12 were 1.88, 2.79, 2.00, and 3.25 g L-1, respectively. Chla. reinhardtii C22 yielded a higher lipid content (51%), with a lower biomass concentration (5.47 g dwt L-1). Chlorella sp. HK12 reached a growth rate of 0.88 day-1 at OD540nm and yielded a biomass concentration of 7.56 g dwt L-1, with a high lipid content of 43%. Gas chromatography/ mass spectrometry analysis indicated that lipid fraction mainly comprises hydrocarbons including palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acids. Our results suggest that Chlorella sp. HK12 is a promising species for biodiesel production, because of its high lipid productivity and a relatively high content of oleic acid.

scholarly journals Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content

Catalysts ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 573
Author(s):  
Patryk Ratomski ◽  
Małgorzata Hawrot-Paw
Keyword(s):  
Chlorophyll A ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Synthetic Medium ◽  
Nutrient Stress ◽  
Stress Conditions ◽  
Microalgal Biomass ◽  
Soxhlet Apparatus ◽  
Aquaculture Wastewater ◽  
Cellular Components

Microalgal biomass and its cellular components are used as substrates for the production of fuels. A valuable group among the components of microalgal biomass is lipids, which act as a precursor for the production of biodiesel in the transesterification process. Some methods, including the creation of stressful conditions, are applied to increase the accumulation of lipids. This study aimed to determine the effect of limited nutrient access on the growth and development of the microalga Chlorella vulgaris and the amount of lipids stored in its cells. Aquaculture wastewater (AWW) was used in the study as a source of nutrients at doses of 20%, 40%, 60%, 80% and 100%. The amount of microalgal biomass, optical density, lipid content after extraction of the biomass in Soxhlet apparatus and chlorophyll a content were determined. It was observed that the microalgae efficiently used the nutrients contained in the AWW. The largest amount of biomass was obtained in AWW80 (727 ± 19.64 mg·L−1). The OD680 (0.492 ± 0.00) determined under the same conditions was almost five times higher in AWW than in the synthetic medium. Under nutrient-stress conditions, the content of lipids in biomass ranged from 5.75% (AWW80) to 11.81% (AWW20). The highest content of chlorophyll a in microalgal cells was obtained in AWW20 (206 ± 11.33 mg∙m−3).

Production of Microalgal Biomass with High Lipid Content and Their Catalytic Processing Into Biodiesel: a Review

2019 ◽  
Vol 11 (4) ◽  
pp. 349-359 ◽  
Author(s):  
A. V. Piligaev ◽  
K. N. Sorokina ◽  
Yu. V. Samoylova ◽  
V. N. Parmon
Keyword(s):  
Lipid Content ◽  
Microalgal Biomass ◽  
High Lipid Content ◽  
High Lipid ◽  
Catalytic Processing

Mixed Trophic State Production Process for Microalgal Biomass with High Lipid Content for Generating Biodiesel and Biogas

2014 ◽  
Vol 7 (4) ◽  
pp. 1174-1185 ◽  
Author(s):  
Pavlo Bohutskyi ◽  
Thomas Kula ◽  
Ben A. Kessler ◽  
Yongseok Hong ◽  
Edward J. Bouwer ◽  
...  
Keyword(s):  
Production Process ◽  
Lipid Content ◽  
Trophic State ◽  
Microalgal Biomass ◽  
High Lipid Content ◽  
High Lipid ◽  
State Production

scholarly journals GrowingChlorella vulgarisin Photobioreactor by Continuous Process Using Concentrated Desalination: Effect of Dilution Rate on Biochemical Composition

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ângelo Paggi Matos ◽  
Regina Coeli de Oliveira Torres ◽  
Luiz Rodrigo Ito Morioka ◽  
Elisa Helena Siegel Moecke ◽  
Kepler Borges França ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Lipid Content ◽  
Continuous Process ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Dry Mass ◽  
Microalgal Biomass ◽  
Steady State Condition

Desalination wastewater, which contains large amount of salt waste, might lead to severely environmental pollution. This study evaluated the effect of dilution rate (0.1≤D≤0.3day−1) on microalgal biomass productivity, lipid content, and fatty acid profile under steady-state condition ofChlorella vulgarissupplemented with concentrated desalination. Continuous culture was conducted for 55 days. Results show that the biomass productivity (Px) varied from 57 to 126 mg L−1 d−1(dry mass) when the dilution rate ranged from 0.1 to 0.3 day−1. At lowest dilution rate (D=0.1day−1), the continuous culture regime ensured the highest values of maximum biomass concentration (Xm=570±20 mL−1) and protein content (52%). Biomass lipid content was an increasing function ofD. The most abundant fatty acids were the palmitic (25.3±0.6%) atD=0.1day−1and the gamma-linolenic acid (23.5±0.1%) atD=0.3day−1ones. These fatty acids present 14 to 18 carbons in the carbon chain, being mainly saturated and polyunsaturated, respectively. Overall, the results show that continuous culture is a powerful tool to investigate the cell growth kinetics and physiological behaviors of the algae growing on desalination wastewater.

Upgrading Wastewater Treatment Plants for Biological Nutrient Removal

1990 ◽  
Vol 22 (7-8) ◽  
pp. 53-60 ◽  
Author(s):  
B. Rabinowitz ◽  
T. D. Vassos ◽  
R. N. Dawson ◽  
W. K. Oldham
Keyword(s):  
Wastewater Treatment ◽  
Nutrient Removal ◽  
Wastewater Treatment Plants ◽  
Design Flow ◽  
Biological Nutrient Removal ◽  
Nitrogen And Phosphorus ◽  
Conventional Activated Sludge ◽  
Recent Developments ◽  
Removal Technology ◽  
Biological Nitrogen

A brief review of recent developments in biological nitrogen and phosphorus removal technology is presented. Guidelines are outlined of how current understanding of these two removal mechanisms can be applied in the upgrading of existing wastewater treatment plants for biological nutrient removal. A case history dealing with the upgrading of the conventional activated sludge process located at Penticton, British Columbia, to a biological nutrient removal facility with a design flow of 18,200 m3/day (4.0 IMGD) is presented as a design example. Process components requiring major modification were the headworks, bioreactors and sludge handling facilities.

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