scholarly journals Analysis of the Bioaugmentation Potential of Pseudomonas putida OR45a and Pseudomonas putida KB3 in the Sequencing Batch Reactors Fed with the Phenolic Landfill Leachate

Water ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 906
Author(s):  
Justyna Michalska ◽  
Artur Piński ◽  
Joanna Żur ◽  
Agnieszka Mrozik
Keyword(s):  
Pseudomonas Putida ◽  
Landfill Leachate ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
High Toxicity ◽  
High Enzymatic Activity ◽  
Wastewater Treatment Systems ◽  
Nonspecific Esterases

The treatment of landfill leachate could be challenging for the biological wastewater treatment systems due to its high toxicity and the presence of poorly biodegradable contaminants. In this study, the bioaugmentation technology was successfully applied in sequencing batch reactors (SBRs) fed with the phenolic landfill leachate by inoculation of the activated sludge (AS) with two phenol-degrading Pseudomonas putida OR45a and Pseudomonas putida KB3 strains. According to the results, the SBRs bioaugmented with Pseudomonas strains withstood the increasing concentrations of the leachate. This resulted in the higher removal efficiency of the chemical oxygen demand (COD) of 79–86%, ammonia nitrogen of 87–88% and phenolic compounds of 85–96% as compared to 45%, 64%, and 50% for the noninoculated SBR. Simultaneously, the bioaugmentation of the AS allowed to maintain the high enzymatic activity of dehydrogenases, nonspecific esterases, and catalase in this ecosystem, which contributed to the higher functional capacity of indigenous microorganisms than in the noninoculated AS. Herein, the stress level experienced by the microorganisms in the SBRs fed with the leachate computed based on the cellular ATP measurements showed that the abundance of exogenous Pseudomonas strains in the bioreactors contributed to the reduction in effluent toxicity, which was reflected by a decrease in the stress biomass index to 32–45% as compared to the nonbioaugmented AS (76%).

Co-treatment of landfill leachate in laboratory-scale sequencing batch reactors: analysis of system performance and biomass activity by means of respirometric techniques

2014 ◽  
Vol 69 (6) ◽  
pp. 1267-1274 ◽  
Author(s):  
M. Capodici ◽  
D. Di Trapani ◽  
G. Viviani
Keyword(s):  
Landfill Leachate ◽  
Oxygen Uptake Rate ◽  
Oxygen Demand ◽  
Domestic Wastewater ◽  
Ammonia Nitrogen ◽  
Synthetic Wastewater ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Population Average ◽  
Biomass Activity

Aged or mature leachate, produced by old landfills, can be very refractory; for this reason mature leachate is difficult to treat alone, but it can be co-treated with sewage or domestic wastewater. The aim of the study was to investigate the feasibility of leachate co-treatment with synthetic wastewater, in terms of process performance and biomass activity, by means of respirometric techniques. Two sequencing batch reactors (SBRs), named SBR1 and SBR2, were fed with synthetic wastewater and two different percentages of landfill leachate (respectively 10% and 50% v v−1 in SBR1 and SBR2). The results showed good chemical oxygen demand (COD) removal efficiency for both reactors, with average COD removals equal to 91.64 and 89.04% respectively for SBR1 and SBR2. Furthermore, both SBRs showed good ammonia-nitrogen (AN) removal efficiencies, higher than 60%, thus confirming the feasibility of leachate co-treatment with a readily biodegradable wastewater. Significant respiration rates were obtained for the heterotrophic population (average values of maximum oxygen uptake rate equal to 37.30 and 56.68 mg O2 L−1 h−1 respectively for SBR1 and SBR2), thus suggesting the feasibility of leachate co-treatment with synthetic wastewater.

scholarly journals The comparison of efficiency of Fenton and photo-Fenton treatment of stabilised landfill leachate / Porównanie efektywności oczyszczania odcieków z ustabilizowanego składowiska odpadów komunalnych w procesie Fentona i foto-Fentona

2015 ◽  
Vol 26 (3) ◽  
pp. 49-53 ◽  
Author(s):  
Anna Kwarciak-Kozłowska ◽  
Aleksandra Krzywicka
Keyword(s):  
Landfill Leachate ◽  
Fenton Reaction ◽  
Ferrous Sulphate ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Ammonia Removal ◽  
Mass Ratio ◽  
Leachate Treatment ◽  
Initial Ph ◽  
Removal Efficiencies

Abstract The goal of this article was to compare the efficiency of Fenton and photo-Fenton reaction used for stabilised landfill leachate treatment. The mass ratio of COD:H2O2 was fixed to 1:2 for every stages. The dose of reagents (ferrous sulphate/hydrogen peroxide) was different and ranged from 0.1 to 0.5. To determine the efficiency of treatment, the BOD (biochemical oxygen demand COD (chemical oxygen demand), TOC (total organic carbon) , ammonia nitrogen and BOD/COD ratio was measured. The experiment was carried out under the following conditions: temperature was 25ºC, the initial pH was adjusted to 3.0. Every processes were lasting 60 minutes. The most appropriate dose of reagents was 0.25 (Fe2+/H2O2). It was found that the application of UV contributed to increase of COD, TOC and ammonia removal efficiencies by an average of 14%.

scholarly journals Performance and Biomass Characteristics of SBRs Treating High-Salinity Wastewater at Presence of Anionic Surfactants

2020 ◽  
Vol 17 (8) ◽  
pp. 2689 ◽  
Author(s):  
Huiru Li ◽  
Shaohua Wu ◽  
Chunping Yang
Keyword(s):  
Extracellular Polymeric Substances ◽  
High Salinity ◽  
Anionic Surfactants ◽  
Sodium Dodecyl ◽  
Ammonia Nitrogen ◽  
Sodium Dodecylbenzene Sulfonate ◽  
Batch Reactors ◽  
Sequencing Analysis ◽  
Sequencing Batch Reactors ◽  
Improper Use

Sodium dodecylbenzene sulfonate (SDBS) and sodium dodecyl sulfate (SDS), as two anionic surfactants, have diffused into environments such as surface water and ground water due to extensive and improper use. The effects on the removal performance and microbial community of sequencing batch reactors (SBRs) need to be investigated in the treatment of saline wastewater containing 20 g/L NaCl. The presence of SDS and SDBS could decrease the removal efficiencies of ammonia nitrogen and total phosphorus, and the effect of SDS was more significant. The effect of surfactants on the removal mainly occurred during the aeration phase. Adding SDS and SDBS can reduce the content of extracellular polymeric substances (EPS). In addition, SDS and SDBS also can reduce the inhibition of high salinity on sludge activity. A total of 16 s of rRNA sequencing analysis showed that the addition of surfactants reduced the diversity of microbial communities; besides, the relative abundance value of the dominant population Proteobacteria increased from 91.66% to 97.12% and 93.48% when SDS and SDBS were added into the system, respectively.

Powdered ZELIAC augmented sequencing batch reactors (SBR) process for co-treatment of landfill leachate and domestic wastewater

2014 ◽  
Vol 139 ◽  
pp. 1-14 ◽  
Author(s):  
Amin Mojiri ◽  
Hamidi Abdul Aziz ◽  
Nastaein Q. Zaman ◽  
Shuokr Qarani Aziz ◽  
Mohammad Ali Zahed
Keyword(s):  
Landfill Leachate ◽  
Domestic Wastewater ◽  
Batch Reactors ◽  
Sequencing Batch Reactors

Occurrence of Microthrix parvicella in sequencing batch reactors

2014 ◽  
Vol 69 (10) ◽  
pp. 1984-1995 ◽  
Author(s):  
Lana Mallouhi ◽  
Ute Austermann-Haun
Keyword(s):  
Activated Sludge ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
Oxygen Demand ◽  
Volume Index ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Microthrix Parvicella

Sequencing batch reactors (SBRs) are known for high process stability and usually have a good sludge volume index (SVI). Nevertheless, in many SBRs in Germany for municipal wastewater treatment, scum and foam problems can occur, and SVI can be larger than 200 mL/g. The microscopic investigations of the activated sludge from plants with nitrogen and phosphorus removal have shown that Microthrix parvicella is dominant in the activated sludge in most of them. Studies showed that the optimum growth of M. parvicella is performed at a high sludge age (>20 d) and low sludge load in the range of 0.05–0.2 kg of biochemical oxygen demand per kg of total suspended solids per day (kg BOD5/(TSS·d)). The investigations in 13 SBRs with simultaneous aerobic sludge stabilization (most of them are operated with a system called differential internal cycle strategy sequential batch reactor (DIC-SBR)) show that M. parvicella is able to grow in sludge loads less than 0.05 kg BOD5/(kg TSS·d) as well. To optimize the operation of those SBRs, long cycle times (8–12 h) and dosing of iron salts to eliminate long-chain fatty acids are both recommended. This leads to better SVI and keeps M. parvicella at a low frequency.

Effect of sludge discharge positions on steady-state aerobic granules in sequencing batch reactor (SBR)

2012 ◽  
Vol 66 (8) ◽  
pp. 1722-1727 ◽  
Author(s):  
Lin Liu ◽  
Da-Wen Gao ◽  
Hong Liang
Keyword(s):  
Steady State ◽  
Batch Reactor ◽  
Oxygen Demand ◽  
Aerobic Granule ◽  
Stable Operation ◽  
Batch Reactors ◽  
Aerobic Granules ◽  
Sequencing Batch Reactors ◽  
Discharge Location

We have investigated the effect of sludge discharge location on the steady-state aerobic granules in sequencing batch reactors (SBRs). Two SBRs were operated concurrently with the same sludge retention time using sludge discharge ports at: (a) the reactor bottom in R1; and (b) the reactor middle-lower level in R2. Results indicate that both reactors could maintain sludge granulation and stable operation, but the two different sludge discharge methods resulted in significantly different aerobic granule characteristics. Over 30 days, the chemical oxygen demand (COD) removal of the two reactors was maintained at similar levels (above 96%), and typical bioflocs were not observed. The average aerobic granule size in R2 was twice that in R1, as settling velocity increased in proportion to size increment. Meanwhile, the production yields of polysaccharide and protein content in R2 were always higher than those in R1. However, due to mass transfer limitations and the presence of anaerobes in the aerobic granule cores, larger granules had a tendency to disintegrate in R2. Thus, we conclude that a sludge discharge port situated at the reactor bottom is beneficial for aerobic granule stability, and enhances the potential for long-term aerobic granule SBR operation.

scholarly journals Comparative Study on Advanced Nitrogen Removal of Landfill Leachate Treated by SBR and SBBR

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3240
Author(s):  
Jinfeng Jiang ◽  
Liang Ma ◽  
Lianjie Hao ◽  
Daoji Wu ◽  
Kai Wang
Keyword(s):  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Landfill Leachate ◽  
Batch Reactor ◽  
Nitrogen Concentration ◽  
Carbon Sources ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Two Systems ◽  
Advanced Nitrogen Removal

In order to achieve advanced nitrogen removal from landfill leachate without the addition of external carbon sources, a Sequencing Batch Reactor (SBR) and a Sequencing Biofilm Batch Reactor (SBBR) were proposed for the treatment of actual landfill leachate with ammonia nitrogen (NH4+-N) and chemical oxygen demand (COD) concentrations of 1000 ± 100 mg/L and 4000 ± 100 mg/L, respectively. The operating modes of both systems are anaerobic–aerobic–anoxic. After 110 days of start-up and biomass acclimation, the effluent COD and the total nitrogen (TN) of the two systems were 650 ± 50 mg/L and 20 ± 10 mg/L, respectively. The removal rates of COD and total nitrogen could reach around 85% and above 95%, respectively. Therefore, advanced nitrogen removal was implemented in landfill leachate without adding any carbon sources. After the two systems were acclimated, nitrogen removing cycles of SBR and SBBR were 24 h and 20 h, respectively. The nitrogen removing efficiency of SBBR was improved by 16.7% in comparison to SBR. In the typical cycle of the two groups of reactors, the nitrification time of the system was the same, which was 5.5 h, indicating that although the fiber filler occupied part of the reactor space, it had no significant impact on the nitrification performance of the system. At the end of aeration, the internal carbon source content of sludge of SBBR was equivalent to that of the SBR system. However, the total nitrogen concentration of SBBR was only 129 mg/L, which is 33.8% lower than that of SBR at 195 mg/L. The main reason was that biofilm enhanced the simultaneous nitrification and denitrification (SND) effect of the system.

scholarly journals Treatment of Leachate Using Up-Flow Anaerobic Sludge Blanket Reactors/Vertical Flow Subsurface Constructed Wetlands

2020 ◽  
Vol 27 (1) ◽  
pp. 129-137
Author(s):  
Şevket Tulun
Keyword(s):  
Total Nitrogen ◽  
Landfill Leachate ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Organic Content ◽  
Ammonia Removal ◽  
Anaerobic Sludge ◽  
Vertical Flow ◽  
Physico Chemical ◽  
Anaerobic Sludge Blanket

AbstractThe composition of local solid waste consists mainly of biodegradable waste with high moisture and organic content. After being landfilled, the waste decomposes through a series of combined physico-chemical and biological processes, resulting in the generation of landfill leachate. Unless treated properly, the leachate poses a serious threat to the environment and to public health. In this study, the use of an engineered system consisting of an up-flow anaerobic sludge blanket reactor and a vertical flow subsurface constructed wetland for the treatment of landfill leachate was investigated. The leachate obtained from a landfill facility in Aksaray, Turkey was fed into both systems and laboratory tests showed that, over the 6-week study period, the systems were able to efficiently remove chemical oxygen demand (88.6 %) and total nitrogen (80.7 %). The results of this study suggested that Typha angustifolia significantly increased the removal of total nitrogen. The higher ammonia removal occurred in the anaerobic system and also the removal efficiency increased in planted bed, it is presumed to be the result of the ammonia nitrogen uptake by the roots of the plant.

scholarly journals Perspectives of intracellular polymers in functional evaluation of the microbes for EBPR

2021 ◽  
Author(s):  
Ghazal Srivastava ◽  
Absar Ahmad Kazmi
Keyword(s):  
Evaluation Studies ◽  
Oxygen Demand ◽  
Cyclic Behavior ◽  
Batch Reactors ◽  
Functional Evaluation ◽  
Sequencing Batch Reactors ◽  
Biological Phosphorus Removal ◽  
Biochemical Processes ◽  
Sudan Black B ◽  
Wastewater Quality

Abstract To substantiate and interpret the performance of the Enhanced Biological Phosphorus Removal (EBPR) processes with simultaneous nitrogen removal in five full-scale sequencing batch reactors (SBR) systems (with or without pre-anoxic/anaerobic selector) across India, conventional microscopic examinations were performed. Regular examining and cyclic behavior evaluation studies specified that these systems worked for EBPR with effectiveness depending on the wastewater quality and operational steadiness. Treatment with Neisser stain for identifying polyphosphates (poly-P) and Sudan black B stain for observing poly-β-hydroxybutyrates (PHB) granules showed that the enriched biomass of the SBR plants was very diverse concerning morphology, residing populations of traditional rod-shaped PAOs, tetrad (or Sarcina-like cells) forming organisms (submitted as TFOs instead of GAOs), diplococci-shaped cells, and staphylococci-like clustered populations (CC), including few filaments which correlate well with biochemical processes undergoing in SBR plants. SBR plants with readily biodegradable chemical oxygen demand (rbCOD) fraction in COD > 16% and rbCOD/TP ∼10–20 in Varanasi, Mumbai, and Gurgaon, respectively, have performed for >20% EBPR (∼77.8%, ∼76.6%, and ∼84.8% TP removal, respectively) as well as >85% Simultaneous Nitrification and Denitrification (SND). This study can open novel dimensions for optimization by relating microscopic observations (qualitative examination) with the processes undergoing in the plants under varied physicochemical parameters.

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