scholarly journals Efecto de diferentes sustratos en el crecimiento vegetativo de vitroplantas de piña (Anana comosus L. Merr) var. Perolera

2020 ◽  
pp. 21
Author(s):  
Miguel Ángel Cetre Cortes ◽  
Jorge Arturo Fernández Vélez ◽  
Liliana Corozo Quiñónez
Keyword(s):  
Vegetative Growth ◽  
Block Design ◽  
Ananas Comosus ◽  
Tropical Species ◽  
High Survival ◽  
Scale Production ◽  
Ex Vitro ◽  
River Sand ◽  
Number Of Leaves

Effect of different substrates on the vegetative growth of pineapple (Anana comosus L. Merr) var. Perolera Resumen Ananas comosus L, es una de las especies tropicales de mayor importancia comercial de la familia Bromeliácea, debido a la fragancia, dulzor de su fruto y a la aceptación por parte de los consumidores en el mundo. La propagación convencional por sí sola no proporciona el material de plantación limpio y adecuado que exigen los países productores de piña para expandir los cultivares a nuevas zonas. La multiplicación in vitro se ha convertido en una técnica prometedora para la producción a gran escala. Sin embargo, las pérdidas en la etapa de aclimatación son críticas en el proceso de micropropagación. Por ello, el objetivo del experimento fue evaluar el efecto de diferentes sustratos en el crecimiento vegetativo de vitroplantas de piña (Anana comosus L.). Se utilizó un diseño de bloques completamente al azar con 4 tratamientos que incluyeron sustratos de composición química diferente más un testigo con arena de río. El porcentaje de sobrevivencia se evaluó a los 10 días después del trasplante (DDT), mientras que la altura de planta y número de hojas se evaluaron a los 30 y 90 DDT. En las variables altura y número de hojas, se realizó un análisis de varianza entre los tratamientos (sustratos) y adicionalmente se realizó un contraste ortogonal entre los sustratos vs el testigo (arena de río). La comparación de medias para las variables con significancia estadística se realizó con la prueba de Duncan al 5% de probabilidad. Se presentaron altas tasas de supervivencia en todos los tratamientos, y un efecto significativo de los sustratos en la altura y número de hojas en comparación con el tratamiento testigo. Palabras clave: Aclimatación; ex vitro; sustratos. Abstract Ananas comosus L, is one of the most commercially important tropical species of the Bromeliad family, due to the fragrance, sweetness of its fruit and its acceptance by consumers around the world. Conventional propagation alone does not provide the clean and adequate planting material required by pineapple producing countries to expand cultivars to new areas. In vitro multiplication has become a promising technique for large-scale production. However, losses at the acclimatisation stage are critical in the micropropagation process. Therefore, the aim of the experiment was to evaluate the effect of different substrates on the vegetative growth of pineapple (Anana comosus L.) glass plants. A completely randomized block design was used with 4 treatments that included substrates of different chemical composition plus a control with river sand. The percentage of survival was evaluated at 10 days after transplanting (DDT), while plant height and number of leaves were evaluated at 30 and 90 DDT. For the variables height and number of leaves, an analysis of variance between the treatments (substrates) and additionally an orthogonal contrast between the substrates vs. the control (river sand) was carried out. The comparison of means for the variables with statistical significance was carried out with the Duncan test at 5% probability. There were high survival rates in all treatments, and a significant effect of the substrates on the height and number of leaves in comparison with the control treatment. Keywords: Acclimatisation; ex vitro; substrates.

scholarly journals In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size

2021 ◽  
Vol 16 (1) ◽  
pp. 69-76
Author(s):  
A A Waman ◽  
P Bohra ◽  
R Karthika Devi ◽  
J Pixy
Keyword(s):  
Carbon Source ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Tropical Species ◽  
Scale Production ◽  
Ex Vitro ◽  
In Vitro Multiplication ◽  
Planting Material

Mango ginger (Curcuma mangga Valeton & Zijp.) is an underutilized rhizomatous species that has been valued in tropical Asian countries as a source of vegetable, spice, salad, medicine, and essential oil. This species is hardy and requires less care for obtaining good yields. Rhizomes are the commonly used propagules for the species, which are also the economic part of the crop. Huge quantity of seed rhizomes is required to promote this crop in larger areas. An efficient in vitro multiplication protocol is one of the options to meet the planting material requirement. Effects of carbon source (glucose, fructose and sucrose) and concentration (1 and 3%, w/v), cytokinins (BAP and meta topolin) and concentration (1 mg/L and 2 mg/L), size of explants (one/ two/ three bud) and IBA treatment (0, 250, 500 and 1,000 mg/L) for concurrent ex vitro rooting cum hardening were studied. Results revealed that for facilitating efficient multiplication, the medium should be supplemented with glucose (3%) as a carbon source and meta topolin (1 mg/L) as cytokinin. Two-bud explant should be used for subculture as it promoted superior shoot proliferation. Concurrent ex vitro rooting cum hardening was possible even without auxin treatment. The present protocol could be useful for large-scale production of quality planting material of this underexploited tropical species.

Long-term in vitro Storage Technique of Valuable Birch Genotypes and Plant Production on its Basis

2019 ◽  
pp. 57-67
Author(s):  
T.M. Tabatskaya ◽  
N.I. Vnukova
Keyword(s):  
Plant Production ◽  
High Survival ◽  
Ex Vitro ◽  
Regenerative Capacity ◽  
In Vitro Storage ◽  
Interspecific Differences ◽  
Regenerated Plants ◽  
Growing Conditions

A technique for the long-term (up to 27 years) in vitro storage of valuable birch genotypes under normal (25 °C, 2.0 klx, 16-h day and 8-h night) and low temperature (4 °C, 0.5 klx, 6-h day and 18-h night) growing conditions on hormone-free media has been described. The study explored for the first time the influence of different strategies to store the clones of Betula pubescens and B. pendula var. сarelica (6 genotypes) on the regenerative capacity of collection samples, adaptive potential of regenerated plants and plant production by the in vitro and ex vitro techniques. It was established that both storage strategies provided a persistently high survival rate (82-100%) and regenerative capacity of in vitro shoots (the multiplication coefficient of 4.2-6.3 and rhizogenic activity of 90-100%). The clones retained their characteristics of height growth under the in vitro and ex vitro conditions, and demonstrated intraclonal homogeneity and lack of signs of somaclonal variability. The plants showed substantial interspecific differences at the stage of multiplication and transfer to the greenhouse. The highest percentage of acclimated plants (75-98% depending on the clone genotype) was obtained after planting of micro plants straight in the greenhouse, which simplified the technology and made plant production less costly. long-term in vitro storage, birch, species, genotype, micropropagation, ex vitro adaptation, plant material

scholarly journals Biofertlizer Lumbrical improves the growth and ex vitro acclimatization of micropropagated pear plants

2021 ◽  
Vol 22 (1) ◽  
pp. 17-30
Author(s):  
Nataliya Dimitrova ◽  
Lilyana Nacheva ◽  
Małgorzata Berova ◽  
Danuta Kulpa
Keyword(s):  
Woody Species ◽  
Photosynthetic Photon Flux Density ◽  
Stem Length ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Ex Vitro ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Number Of Leaves

In vitro micropropagation of plants is highly useful for obtaining large quantities of planting material with valuable economic qualities. However, plantlets grow in vitro in a specific environment and the adaptation after the transfer to ex vitro conditions is difficult. Therefore, the acclimatization is a key step, which mostly determines the success of micropropagation. The aim of this investigation was to study the effect of the biofertlizer Lumbrical on ex vitro acclimatization of micropropagated pear rootstock OHF 333 (Pyrus communis L.). Micropropagated and rooted plantlets were potted in peat and perlite (2:1) mixture with or without Lumbrical. They were grown in a growth chamber at a temperature of 22±2 °C and photoperiod of 16/8 hours supplied by cool-white fluorescent lamps (150 µmol m-2 s-1 Photosynthetic Photon Flux Density, PPFD). The plants were covered with transparent foil to maintain the high humidity, and ten days later, the humidity was gradually decreased. Biometric parameters, anatomic-morphological analyses, net photosynthetic rate and chlorophyll a fluorescence (JIP test) were measured 21 days after transplanting the plants to ex vitro conditions. The obtained results showed that the plants, acclimatized ex vitro in the substrate with Lumbrical, presented better growth (stem length, number of leaves, leaf area and fresh mass) and photosynthetic characteristics as compared to the control plants. This biostimulator could also be used to improve acclimatization in other woody species

scholarly journals PROPAGACIÓN CLONAL in vitro DE PIÑA (Ananas comosus L. Merr) VARIEDADES CHAMPAKA Y HAWAIANA

2008 ◽  
Vol 1 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Silvia Gicela Saucedo Aguiar ◽  
Edgar Varas Giler ◽  
Fred Carmigniani Castro ◽  
Luís Ramos Gavilanes
Keyword(s):  
Ananas Comosus ◽  
Ex Vitro

Este trabajo fue realizado con la finalidad de establecer un protocolo para la propagación in vitro de piña (Ananas comosus (L) Merr), así como determinar las mejores concentraciones de reguladores de crecimiento en el establecimiento, multiplicación, enraizamiento y aclimatación ex vitro. Para el establecimiento de piña var. Champaka y Hawaiana la mejor concentración fue en un medio de cultivo conformado por la totalidad de las sales del MS suplementado con BAP (0.5 mg L-1); AIB (1.0 mg L-1) y ANA (1.0 mg L-1) obteniéndose el 47 y 56 % de supervivencia respectivamente. Para la proliferación de brotes la mejor concentración fue en un medio de cultivo conformado por la totalidad de las sales del MS suplementado con 3.5 y 4.0 mg L-1 de BAP en la var. Champaka y la var. Hawaiana con 3.5 mg L-1 de BAP. Para el enraizamiento ex vitro y aclimatación la mejor respuesta al número de raíces se presentó en el testigo (sin hormona) var. hawaiana con un promedio de 7.25. La mayor longitud se evidenció en el testigo (sin hormona) con 3.55 cm se obtuvo una sobrevivencia de 93.75 % para la var. Hawaiana y 79.16 % para la var. Champaka. Los mejores resultados en el enraizamiento y aclimataciones ex vitro de vitroplantas de piña se obtuvieron con el testigo en la variedad Champaka y con la auxina ANA (ácido naptalenacetico) 50 mg L-1 en la var. Hawaiana.

Application of Potassium Fertilizer and Chicken Coop Fertilizer Against Growth and Production of Shallots (Allium ascalanicum L.)

Nabatia ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Andy Pradana ◽  
Al Machfudz WDP
Keyword(s):  
Growth Phase ◽  
Vegetative Growth ◽  
Growth Parameters ◽  
Block Design ◽  
Dry Weight ◽  
Chicken Manure ◽  
Fertilizer Treatment ◽  
Potassium Fertilizer ◽  
Wet Weight ◽  
Number Of Leaves

This study aims to determine the application of potassium fertilizer and chicken manure to the growth and production of shallots (Allium ascalanicum L.). The study used a randomized block design with 2 factors repeated 3 times. Factor 1: Potassium Fertilizer 40,60,80 kg/ha. Factor 2: Chicken Manure 5,10,15 tons/ha. The results showed that the application of potassium fertilizer and chicken manure did not interact with either the growth phase or the production phase of the plant on all observation variables, while the potassium fertilizer treatment showed significantly different results only on the plant vegetative growth parameters, namely the number of leaves at the age of 7 days after planting, namely ( 8,111 strands), while the vegetative phase had no significant effect on the number of tubers per clump, wet weight of tubers per clump, wet weight of tubers per plot, dry weight of sun-dried tubers per clump, and dry weight of sun-dried tubers per plot. While the treatment of chicken manure had a significant effect on the vegetative growth phase of plants, namely the length of plants at 28 DAP (30.278 cm) and 35 DAP (32.704 cm), on the number of leaves at 7 DAP (8,000 strands). While the generative phase (60 DAP) significantly affected the number of tubers per clump, namely (5,889 grams), wet weight of tubers per clump (38,333 grams), wet weight of tubers per plot (319,000 grams), dry weight of sun-dried tubers per clump. namely (23.074 grams), and the dry weight of sun-dried tubers per plot (191.889 grams).

scholarly journals Facilitate Seed Germination of the Golden Shower Tree (Cassia fistula) in vitro Using TiO2 Nanoparticles and Scarification Treatments

2016 ◽  
Vol 8 (9) ◽  
pp. 168
Author(s):  
Fatemeh Feizi ◽  
Mousa Mousavi
Keyword(s):  
Seed Germination ◽  
Dry Weight ◽  
Hot Water ◽  
Peat Moss ◽  
Ex Vitro ◽  
Salt Mixture ◽  
Cassia Fistula ◽  
Number Of Leaves ◽  
Tree Seeds

<p>The main propagation method of <em>Cassia fistula</em> is sowing seeds. The seed germination is usually low because of its impermeable hard coat. Therefore, this experiment evaluated the effects of TiO<sub>2</sub> nanoparticles and scarification methods on seed germination and seedling growth <em>in vitro</em> condition. The tree seeds were treated with, hot water, H<sub>2</sub>SO<sub>4</sub> (36N), and mechanical scarification and culture on ¼ MS salt mixture. The medium was supplemented with different concentrations of TiO<sub>2</sub> nanoparticles. The results showed that the highest percentage and rate of germination was recorded in seeds treated with mechanical scarification. The highest shoot and root dry weight was recorded for seeds treated with mechanical scarification and grown on MS media supplemented with 1.5 mg/ml TiO<sub>2</sub> nanoparticles. TiO<sub>2</sub> nanoparticles did not show any significant effects on the percentage and rate of germination. Different growing soil mixtures had significant effects on the growth of the ex vitro transferred plantlets. Coco peat and peat moss mixture (1:1) was found to be more effective in increasing the number of leaves and root length of the seedlings.</p>

scholarly journals PROPAGACIÓN CLONAL in vitro DE PIÑA (Ananas comosus L. Merr) VARIEDADES CHAMPAKA Y HAWAIANA

2008 ◽  
Vol 1 (1) ◽  
pp. 49
Author(s):  
Silvia Gicela Saucedo Aguiar ◽  
Edgar Varas Giler ◽  
Fred Carmigniani Castro ◽  
Luís Ramos Gavilanes
Keyword(s):  
Ananas Comosus ◽  
Ex Vitro

Este trabajo fue realizado con la finalidad de establecer un protocolo para la propagación in vitro de piña (Ananas comosus (L) Merr), así como determinar las mejores concentraciones de reguladores de crecimiento en el establecimiento, multiplicación, enraizamiento y aclimatación ex vitro. Para el establecimiento de piña var. Champaka y Hawaiana la mejor concentración fue en un medio de cultivo conformado por la totalidad de las sales del MS suplementado con BAP (0.5 mg L-1); AIB (1.0 mg L-1) y ANA (1.0 mg L-1) obteniéndose el 47 y 56 % de supervivencia respectivamente. Para la proliferación de brotes la mejor concentración fue en un medio de cultivo conformado por la totalidad de las sales del MS suplementado con 3.5 y 4.0 mg L-1 de BAP en la var. Champaka y la var. Hawaiana con 3.5 mg L-1 de BAP. Para el enraizamiento ex vitro y aclimatación la mejor respuesta al número de raíces se presentó en el testigo (sin hormona) var. hawaiana con un promedio de 7.25. La mayor longitud se evidenció en el testigo (sin hormona) con 3.55 cm se obtuvo una sobrevivencia de 93.75 % para la var. Hawaiana y 79.16 % para la var. Champaka. Los mejores resultados en el enraizamiento y aclimataciones ex vitro de vitroplantas de piña se obtuvieron con el testigo en la variedad Champaka y con la auxina ANA (ácido naptalenacetico) 50 mg L-1 en la var. Hawaiana.

scholarly journals Acclimatization and growth of ornamental pineapple seedlings under organic substrates

2017 ◽  
Vol 23 (3) ◽  
pp. 257
Author(s):  
Ronan Carlos Colombo ◽  
Vanessa Favetta ◽  
Maria Aparecida da Cruz ◽  
Deived Uilian de Carvalho ◽  
Sergio Ruffo Roberto ◽  
...  
Keyword(s):  
Rice Husk ◽  
Pine Bark ◽  
Ananas Comosus ◽  
Organic Substrates ◽  
Seedling Mortality ◽  
Survival Percentage ◽  
Number Of Leaves ◽  
Physical And Chemical ◽  
The Ideal

The in vitro propagation techniques are commonly used to produce ornamental pineapple seedlings in commercial scale, aiming to attend the growers with genetic and sanitary quality seedlings. However, the choice of the ideal substrate is essential for the acclimatization and growth stage of the seedlings propagated by this technique, since some substrates can increase the seedling mortality and/or limit the seedling growth due to its physical and chemical characteristics. Thus, the aim of this study was to evaluate the acclimatization of ornamental pineapple [Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & Leal] on different substrates. Seedlings with approximately seven centimeters, obtained from in vitro culture, were transplanted into styrofoam trays filled with the following substrates: sphagnum; semi-composed pine bark; carbonized rice husk; sphagnum + semicomposed pine bark; sphagnum + carbonized rice husk; and semi-composed pine bark + carbonized rice husk. Each treatment was replicated five times using 10 plants. At 180 days, there were evaluated the following variables: survival percentage, plant height, number of leaves, leaf area, largest root length, and shoot and root dry matter. The substrate semi-composed pine bark + carbonized rice husk presented the lowest mean (62%) for survival percentage. The semi-composed pine bark and semi-composed pine bark + carbonized rice husk treatments presented significant increments in some evaluated biometric characteristics. The semi-composed pine bark is the most favorable substrate for the A. comosus var. ananassoids acclimatization.

scholarly journals Comparative Growth Performance of Papaya Hybrids and their Parents under Reciprocal Crossing System

2020 ◽  
pp. 167-173
Author(s):  
Suchismita Jena ◽  
T. R. Ahlawat ◽  
A. I. Patel ◽  
A. K. Pandey ◽  
Dharmishtha Patel ◽  
...  
Keyword(s):  
Vegetative Growth ◽  
Growth Parameters ◽  
Block Design ◽  
Germination Percentage ◽  
Internodal Length ◽  
Randomized Block Design ◽  
Growth Performances ◽  
Breeding Programmes ◽  
Number Of Leaves ◽  
Reciprocal Crossing

Papaya is a very popular fruit in the tropical and subtropical regions of the world and has witnessed an increase in demand. This has fuelled an interest to develop region specific hybrids with desirable vegetative growth and vigour.  Hence a field experiment was carried out at College of horticulture and forestry, Navsari Agricultural University in Gujarat during 2018-2020 in randomized block design with three replications to know the mean performance of parents and hybrids for comparative growth performances of papaya. Wherein, thirty papaya hybrids were developed from six morphologically distinct parents through full diallel crossing technique and all the parents and hybrids were evaluated for their germination and growth parameters. The variability was distinct in all hybrids and parents for germination percentage, days taken to germination, periodical plant height and girth, number of leaves, petiole length and internodal distance. Amongst all the parents, Pusa Delicious, CO-8 and Lucknow Local exhibited improved germination, better vegetative growth and dwarf stature, respectively. Among the hybrids, Pusa Delicious x Lucknow Local was found to have dwarf stature with minimum internodal length however, the reciprocal cross: Lucknow Local x Pusa Delicious recorded maximum plant girth and number of leaves. Based on their mean performance, these parents can be used for further breeding programmes and hybrids could be exploited for cultivation.

scholarly journals Morphological Development of Lingonberry as Affected by In Vitro and Ex Vitro Propagation Methods and Source Propagule

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 760-763 ◽  
Author(s):  
Samir C. Debnath
Keyword(s):  
Vegetative Growth ◽  
Nutrient Medium ◽  
Growth And Development ◽  
Shoot Proliferation ◽  
Morphological Development ◽  
Stem Cuttings ◽  
Ex Vitro ◽  
Propagation Methods ◽  
In Vitro Shoot Proliferation

The growth and development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants and by shoot regeneration from excised leaves of micropropagated shoots, were studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. After 3 years of growth, the in vitro-derived plants produced more stems, leaves, and rhizomes than the conventional cuttings which rarely produced rhizomes. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favor rhizome production. This increase in vegetative growth and rhizome yield of in vitro-derived plants over stem cuttings varied among genotypes.

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