Acclimatization and growth of ornamental pineapple seedlings under organic substrates

The in vitro propagation techniques are commonly used to produce ornamental pineapple seedlings in commercial scale, aiming to attend the growers with genetic and sanitary quality seedlings. However, the choice of the ideal substrate is essential for the acclimatization and growth stage of the seedlings propagated by this technique, since some substrates can increase the seedling mortality and/or limit the seedling growth due to its physical and chemical characteristics. Thus, the aim of this study was to evaluate the acclimatization of ornamental pineapple [Ananas comosus (L.) Merr. var. ananassoides (Baker) Coppens & Leal] on different substrates. Seedlings with approximately seven centimeters, obtained from in vitro culture, were transplanted into styrofoam trays filled with the following substrates: sphagnum; semi-composed pine bark; carbonized rice husk; sphagnum + semicomposed pine bark; sphagnum + carbonized rice husk; and semi-composed pine bark + carbonized rice husk. Each treatment was replicated five times using 10 plants. At 180 days, there were evaluated the following variables: survival percentage, plant height, number of leaves, leaf area, largest root length, and shoot and root dry matter. The substrate semi-composed pine bark + carbonized rice husk presented the lowest mean (62%) for survival percentage. The semi-composed pine bark and semi-composed pine bark + carbonized rice husk treatments presented significant increments in some evaluated biometric characteristics. The semi-composed pine bark is the most favorable substrate for the A. comosus var. ananassoids acclimatization.

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Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.6.1.20-28 ◽

2015 ◽

Vol 6 (1) ◽

pp. 20

Author(s):

Erna Sinaga ◽

Megayani Sri Rahayu ◽

Awang Maharijaya

Keyword(s):

Tissue Culture ◽

Drought Tolerance ◽

In Vitro Selection ◽

Solanum Melongena ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRACTThe objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought tolerance of eggplant and the drought tolerant eggplant accessions. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was arranged in a completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10, and 15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090). The results showed that the addition of PEG to in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG) terhadap pertumbuhan tanaman terung in vitro, mendapatkan konsentrasi PEG yang dapat digunakan untuk seleksi tanaman terung secara in vitro dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Penelitian ini disusun dalam rancangan acak lengkap dua faktor. Faktor pertama adalah konsentrasi PEG terdiri atas 0, 5, 10, dan 15%. Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090). Hasil penelitian menunjukkan bahwa penambahan PEG pada media in vitro memberikan pengaruh nyata dan sangat nyata terhadap persentase hidup eksplan, persentase eksplan berkalus, pertambahan tinggi tunas, dan jumlah daun tanaman terung. Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan

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Regional Substrates of the Brazilian Northeast on Acclimatization and Development Morphological of Minirose

Journal of Agricultural Science ◽

10.5539/jas.v10n6p341 ◽

2018 ◽

Vol 10 (6) ◽

pp. 341

Author(s):

David Correia dos Anjos ◽

Gabrielen De Maria Gomes Dias ◽

Fernando Felipe Ferreyra Hernandez ◽

Josefa Diva Nogueira Diniz

Keyword(s):

In Vitro Culture ◽

Rice Husk ◽

Dry Matter ◽

Good Choice ◽

Morphological Development ◽

Shoot Height ◽

Rosa Chinensis ◽

Survival Percentage ◽

Brazilian Northeast

The transition from heterotrophism to autotrophism in acclimatization is a delicate process for most species. The objective of this work was to evaluate combinations of regional substrates of the Brazilian Northeast in the acclimatization and morphological development of minirose (Rosa chinensis ‘Minima’) seedlings. The experiment was carried out in a greenhouse using seedlings with 30 days of in vitro culture. The treatments were constituted by: 1) dry coconut powder; 2) 75% dry coconut powder + 25% rice husk; 3) 50% dry coconut powder + 50% rice husk; 4) 75% dry coconut powder + 25% charcoal rice husk; 5) 50% dry coconut powder + 50% carbonized rice husk; 6) 100% vermiculite; 7) 50% vermiculite + 50% dry coconut powder; 8) commercial substrate; 9) 75% sand + 25% dry coconut powder and 10) 50% sand + 50% dry coconut powder. After 21 days, the following characteristics were evaluated: survival percentage, leaf number, shoot height, dry matter of shoot and root and total dry matter. The regional materials tested showed a good choice of substrates to be used in the acclimatization of minirose seedlings. The vermiculite presented the worst results in the characteristics evaluated. Dry coconut powder as substrate should be used in mixtures with other materials to provide better acclimatization conditions. The use of 50% dry coconut powder + 50% rice husk provided a best development and growth of minirose seedlings (Rosa chinensis ‘Minima’) than other tested substrates

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PLANT GROWTH OF EGGPLANT (Solanum melongena L.) IN VITRO IN DROUGHT STRESS POLYETHYLENE GLYCOL (PEG)

BIOVALENTIA Biological Research Journal ◽

10.24233/biov.2.1.2016.29 ◽

2016 ◽

Vol 2 (1) ◽

Author(s):

Erna Siaga ◽

Awang Maharijaya ◽

Megayani Sri Rahayu

Keyword(s):

Polyethylene Glycol ◽

Drought Stress ◽

Plant Growth ◽

Solanum Melongena ◽

Dry Land ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves

Drought stress is one of the important issues related to the global warming that demand for the development of drought tolerant crops. Eggplant is one of the agricultural commodities which can be developed in dry land so plant growth of eggplant need to be learned. The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, and to find the drought tolerant eggplant accessions in dry land. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was laid on a completely randomized design with one factor. The factor was eggplant accessions. The results showed that PEG in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Callus in eggplant explants as a way of avoiding drought stress.

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Morpho-physiological changes in pineapple plantlets [Ananas comosus (L.) merr.] during acclimatization

Ciência e Agrotecnologia ◽

10.1590/s1413-70542012000600004 ◽

2012 ◽

Vol 36 (6) ◽

pp. 624-630 ◽

Cited By ~ 5

Author(s):

Ariel Villalobo ◽

Justo González ◽

Ramón Santos ◽

Romelio Rodríguez

Keyword(s):

Relative Humidity ◽

Photosynthetic Activity ◽

Net Photosynthesis ◽

Ananas Comosus ◽

Photon Flux ◽

Physiological Changes ◽

Fresh Weight ◽

Number Of Leaves ◽

Plant Length

Pineapple producing countries lack good quality propagation material to expand cultivars into new areas. Previously, research protocols may increase the offer of high quality plantlets and speed the introduction of new pineapple cultivars. The present work is to evaluate the morpho-physiological changes in plantlets of pineapple [Ananas comosus (L.) Merr. 'MD-2'] during the acclimatization phase. Plantlets were acclimatized under 80% relative humidity, 25.5 °C temperature and photosynthetic photon flux of 400-500 µmol m-2 s-1 as average for 45 d under natural photoperiods. All measurements (plant length, number of leaves and roots, fresh weight, width and length of leaf 'D', net photosynthesis and total transpiration rate) were carried out at the end of in vitro rooting phase coincident with 0 d of acclimatization and at 15, 30 and 45 d thereafter. Photosynthetic activity of in vitro plantlets did not increase during the first 30 d of the acclimatization phase. After 30 d, photosynthetic activity ranged from 5.72 to 9.36 µmol CO2 m-2 s-1 while total transpiration ranged from 6.0 to 1.42 mmol H2O m-2 s-1. During the first 30 days there were no significant differences in number of leaves, length or width of the longest ('D') leaf (cm) or plant length (cm). However, after 45 days plant fresh weight (g), length and width of the 'D' leaf (cm) and root number all increased significantly, while transpiration (mmol H2O m-2 s-1) declined. There were small but significant decreases in chlorophyll a and b (µg g-1 mf.). Increased photosynthetic activity after 30 d shows that the increase in light intensity and the reduction of relative humidity during acclimatization did not constitute inhibitory factors.

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Blueberry Propagation by Minicuttings in Response to Substrates and Indolebutyric Acid Application Methods

Journal of Agricultural Science ◽

10.5539/jas.v10n9p450 ◽

2018 ◽

Vol 10 (9) ◽

pp. 450 ◽

Cited By ~ 2

Author(s):

Ronan C. Colombo ◽

Deived U. de Carvalho ◽

Maria A. da Cruz ◽

Sergio R. Roberto

Keyword(s):

Rice Husk ◽

Root Development ◽

Alcohol Solution ◽

Pine Bark ◽

Indolebutyric Acid ◽

Vaccinium Ashei ◽

Application Method ◽

Significant Difference ◽

Application Methods ◽

The Ideal

The nursery plants supply is one of the most important factors that limites blueberries production in some areas, due to some propagation difficulties observed on this species. In this way, this study aimed to evaluate substrates and IBA application methods on ‘Powderblue’ blueberries (Vaccinium ashei) minicuttings rooting, as well as to verify the rooting development in different periods. The study was divided in two experiments. The first one aimed to evaluate ‘Powderblue’ blueberry minicuttings rooting in three substrates (pine bark, carbonized rice husk and vermiculite) in response to two indolebutyric acid (IBA) application methods: 50% alcohol solution and talc. The second experiment aimed to evaluate ‘Powderblue’ blueberry minicuttings rooting using the best substrate recorded in the first trial (pine bark) and two IBA application methods associated to three periods of rooting access, in order to obtain the ideal rooting period and root development. In the first experiment the rooting percentage presented significant difference among the substrates; the higher percentage of rooted minicuttings occurred in pine bark substrate (68%). Regarding the methods used to IBA application, the talc method was more efficient to promote minicuttings rooting. In the second experiment, it was observed an increase in cutting rooting percentage from 55 to 84%, according to the evaluated seasons; however, the IBA application method did not influence the rooting percentage. Therefore, to produce ‘Powderblue’ blueberry plants from minicuttings it is recommended to use pine bark as substrate and talc as method to IBA application, transplanting the nurseries at 110 days after planting.

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ALTERNATIVES IN MEDIA: FINDING RENEWABLE SUBSTITUTES FOR PEATMOSS

HortScience ◽

10.21273/hortsci.30.3.426a ◽

1995 ◽

Vol 30 (3) ◽

pp. 426a-426

Author(s):

LeAnne Newell ◽

Janet C. Cole

Keyword(s):

Leaf Area ◽

Chemical Properties ◽

Pine Bark ◽

Volume Control ◽

Peat Moss ◽

Recycled Paper ◽

Growing Medium ◽

Number Of Leaves ◽

Physical And Chemical ◽

And Chemical Properties

WetEarth, a processed recycled newspaper product, was used in combination with pine bark, sand, and vermiculite as a growing medium for rose of Sharon and forsythia. Rose of Sharon was taller and had more leaves; more leaf area per plant; and greater leaf, stem, and root dry weights in all media containing recycled paper compared to plants grown in a medium consisting of 3 pine bark: 1 peat moss: 1 sand (by volume) (control). Forsythia grown in the control media were taller than those grown in any medium containing recycled newspaper. There was no difference in number of leaves per plant or leaf area of forsythia, regardless of growing medium. Physical and chemical properties of each medium also were investigated. All media containing recycled newspaper had a higher pH, porosity, and air space than the control medium.

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MULTIPLIKASI TUNAS DAN AKLIMATISASI PEGAGAN (Centella asiatica L.) PERIODE KULTUR LIMA TAHUN

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v14n1.2008.30-35 ◽

2020 ◽

Vol 14 (1) ◽

pp. 30

Author(s):

NATALINI NOVA KRISTINA ◽

DEDI SURACHMAN

Keyword(s):

Rice Husk ◽

Research Result ◽

Shoot Multiplication ◽

Centella Asiatica ◽

Randomized Design ◽

Medicinal Crop ◽

Pedicle Length ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRAK Pegagan (Centella asiatica L.) adalah tanaman obat yang mengandung zat asiaticotik sebagai obat alzaimer dan penghalus kulit. Tanaman ini telah diperbanyak sejak tahun 2000. Penelitian dilakukan di Laboratorium Kultur Jaringan dan Rumah Kaca Kelompok Peneliti Plasma Nutfah dan Pemuliaan, Balai Penelitian Tanaman Obat dan Aromatik, Bogor dari bulan Januari 2000 sampai dengan Juni 2005. Penelitian ini bertujuan untuk melihat daya multiplikasi tunas setiap periode subkultur dimulai dari tahun kedua sampai periode lima tahun. Media yang digunakan adalah MS + BA 0,1 mg/l. Pengamatan dilakukan pada jumlah tunas, jumlah daun dan visual tunas pada umur 2, 3, 4 dan 5 tahun pada dua periode subkultur setiap tahunnya. Penelitian disusun dengan rancangan acak lengkap, masing-masing terdiri atas 10 botol yang merupakan ulangan dan setiap botol terdiri atas 1 eksplan. Untuk re-media terhadap tanaman yang terlihat berubah digunakan media MS + BA (0; 0,1; 0,2; 0,3) mg/l. Selanjutnya untuk perakaran dilakukan pada media MS + IAA (0,1 dan 0,2); MS + NAA (0,1 dan 0,2) mg/l serta MS + IBA (0,1 dan 0,2) mg/l. Plantlet utuh yang terbentuk selanjutnya diaklimatisasi pada media tanah + pupuk kandang dan tanah + sekam dengan perbandingan 1 : 1. Hasil penelitian menunjukkan bahwa daya multiplikasi tunas optimum terjadi pada tahun ketiga, dan memasuki tahun keempat dan kelima menurun yang diiringi dengan perubahan eksplan yang terlihat pada tangkai daun yang terbentuk. Akar terpanjang dan terbanyak yang terbentuk didapat pada media IAA 0,2 mg/l dengan penampilan yang kurus dan rapuh. Keberhasilan aklimatisasi sangat rendah, tetapi plantlet mampu beregenerasi dengan baik dan terlihat tumbuh normal. Dari hasil perbanyakan terlihat bahwa jumlah anakan, jumlah daun, panjang stolon dan jumlah bunga lebih tinggi dibandingkan yang tumbuh pada media sekam, berturut-turut : 6,77; 7,30; 46,50 cm dan 8,31. Sementara pada media sekam komponen yang dominan adalah panjang tangkai daun yakni 9,75 cm. Kata kunci : Pegagan, Centella asiatica L., multiplikasi, tunas, aklima- tisasi, penyimpanan, Jawa Barat ABSRACT Shoot multiplication and acclimatization of gotuloca (Centella asiatica L.) five years after conservation by in vitro culture Gotuloca (Centella asiatica L.) is a medicinal crop containing asiaticotic as alzaimer and skin revitalizer. This crop has been multiplicated in vitro since 2000. This research was carried out in the laboratory and glasshouse of Breeding and Germplasm Group in the Indonesian Medicinal and Aromatic Crops Research Institute (IMACRI) from January 2000 to June 2005. The objective of the research was to find out the viability of shoots multiplication after two year to five year period, with media MS + BA 0.1 mg/l. The parameters observed were the number of shoots, the number of leaves at the age 2, 3, 4 and 5 years from two subculture periods every year. The treatments were arranged in a completely randomized design, each replication consisted of 10 bottles and each bottle consisted of 1 explant. After subculture the ex-plant were re- media in medium MS + BA (0; 0,1; 0,2; 0,3) mg/l. The rooting media before glasshouse were : MS + IAA ( 0,1 and 0,2); MS + NAA ( 0,1 and 0,2) mg/l; and MS + IBA ( 0,1 and 0,2) mg/l. The plantlets formed were acclimatized using soil + cattle manure and soil + rice husk with comparison 1:1. Research result indicated that the optimum viability multiplication was achieved in the third year, and it decreased after the fourth and fifth years with change in explant forming the petiole. The longest and plantlet roots were formed through media IAA 0.2 mg/l with brittle and thin appearance, but the plantlets were able to regenerate better and grow normal. The acclimatization was not very successful but the plantlets could regenerate and grew normally. The multiplication showed that the number of stumps, leaves, stolons and flowers were : 6,77; 7,30; 46,50 cm and 8,31 respectively. In rice husk media the dominant component was pedicle length 9,75 cm. Key words : Gotuloca, Centella asiatica L., multiplication, shoot, acclimatization, conservation, East Jav

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Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n5p2901 ◽

2016 ◽

Vol 37 (5) ◽

pp. 2901

Author(s):

Daniele Brandstetter Rodrigues ◽

Michele Carla Nadal ◽

Samila Silva Camargo ◽

Adriane Marinho de Assis ◽

Márcia Wullf Schuch ◽

...

Keyword(s):

Growth Regulators ◽

Aerial Part ◽

Culture Medium ◽

Coconut Fiber ◽

Indole Butyric Acid ◽

In Vitro Multiplication ◽

Survival Percentage ◽

Number Of Leaves ◽

Points Of View

An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated- various concentrations of 6-benzylaminopurine (BAP) (0.0, 1.0, 2.0, and 3.0 mg L-1) and substrates (agar, vermiculite, and coconut fiber) added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA) (0.0, 0.5, 1.0, and 1.5 mg L-1) were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L-1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture.

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The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.) from Sipahutar North Sumatera In Vitro

International Journal of Biological Research ◽

10.14419/ijbr.v6i2.13699 ◽

2018 ◽

Vol 6 (2) ◽

pp. 23

Author(s):

Arisah Hasanah ◽

Fauziyah Harahap ◽

Ramlan Silaban

Keyword(s):

Root Length ◽

Leaf Length ◽

Ananas Comosus ◽

Root Number ◽

Indole Butyric Acid ◽

Shoot Height ◽

Leaf Emergence ◽

Two Factors ◽

Number Of Leaves

Background: Rooting of pineapple from Sipahutar in vitro is an alternative to obtain superior seeds in large quantities to meet the needs of farmers and consumers.Objective: The purpose of this study was to investigate the effect of Myo-inositol and IBA on pineapple rooting (Ananas comosus L.) from Sipahutar in vitro.Methods: The research method used a completely randomized factorial design with two factors: Factor I: Myo-inositol consisted of [4] treatment concentrations: M0 = 0 g/l, M0.02 = 0.02 g/l, M0.04 = 0.04 gr/l, M0.06 = 0.06 gr/l. Factor II: IBA growth regulators consisted of [3] treatment concentrations: IB0 = 0 ppm, IB1.5 = 1.5 ppm, IB3 = 3 ppm. The parameters observed were time of root emergence, number of roots, number of leaves, time of leaf emergence, number of tillers, and time of its emergence observed each week, while the height of tillers, shoots, and whole shoots, root length, leaf width and leaf length were observed 12 weeks after planting (WAP).Results: The result of the research showed that root time appeared on [3] WAP. The increasing number of leaves and timing of the puppiesin 1 WAP. Myo-inositol had significant effects on root counts in the 0.06 gr/l and root length on Myo-inositol treatment in the 0.04 g/l. IBA did not have a real effect on the entire observation parameters. The interactions of Myo-inositol and IBA had significant effects on root number, number of leaves, leaf length and tiller height.Conclusions: Myo-inositol and IBA had significant effects on root number, leaf number, leaf length and shoot height of pineapple’s explant from Sipahutar.

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Efecto de diferentes sustratos en el crecimiento vegetativo de vitroplantas de piña (Anana comosus L. Merr) var. Perolera

La Técnica: Revista de las Agrociencias. ISSN 2477-8982 ◽

10.33936/la_tecnica.v0i0.2702 ◽

2020 ◽

pp. 21

Author(s):

Miguel Ángel Cetre Cortes ◽

Jorge Arturo Fernández Vélez ◽

Liliana Corozo Quiñónez

Keyword(s):

Vegetative Growth ◽

Block Design ◽

Ananas Comosus ◽

Tropical Species ◽

High Survival ◽

Scale Production ◽

Ex Vitro ◽

River Sand ◽

Number Of Leaves

Effect of different substrates on the vegetative growth of pineapple (Anana comosus L. Merr) var. Perolera Resumen Ananas comosus L, es una de las especies tropicales de mayor importancia comercial de la familia Bromeliácea, debido a la fragancia, dulzor de su fruto y a la aceptación por parte de los consumidores en el mundo. La propagación convencional por sí sola no proporciona el material de plantación limpio y adecuado que exigen los países productores de piña para expandir los cultivares a nuevas zonas. La multiplicación in vitro se ha convertido en una técnica prometedora para la producción a gran escala. Sin embargo, las pérdidas en la etapa de aclimatación son críticas en el proceso de micropropagación. Por ello, el objetivo del experimento fue evaluar el efecto de diferentes sustratos en el crecimiento vegetativo de vitroplantas de piña (Anana comosus L.). Se utilizó un diseño de bloques completamente al azar con 4 tratamientos que incluyeron sustratos de composición química diferente más un testigo con arena de río. El porcentaje de sobrevivencia se evaluó a los 10 días después del trasplante (DDT), mientras que la altura de planta y número de hojas se evaluaron a los 30 y 90 DDT. En las variables altura y número de hojas, se realizó un análisis de varianza entre los tratamientos (sustratos) y adicionalmente se realizó un contraste ortogonal entre los sustratos vs el testigo (arena de río). La comparación de medias para las variables con significancia estadística se realizó con la prueba de Duncan al 5% de probabilidad. Se presentaron altas tasas de supervivencia en todos los tratamientos, y un efecto significativo de los sustratos en la altura y número de hojas en comparación con el tratamiento testigo. Palabras clave: Aclimatación; ex vitro; sustratos. Abstract Ananas comosus L, is one of the most commercially important tropical species of the Bromeliad family, due to the fragrance, sweetness of its fruit and its acceptance by consumers around the world. Conventional propagation alone does not provide the clean and adequate planting material required by pineapple producing countries to expand cultivars to new areas. In vitro multiplication has become a promising technique for large-scale production. However, losses at the acclimatisation stage are critical in the micropropagation process. Therefore, the aim of the experiment was to evaluate the effect of different substrates on the vegetative growth of pineapple (Anana comosus L.) glass plants. A completely randomized block design was used with 4 treatments that included substrates of different chemical composition plus a control with river sand. The percentage of survival was evaluated at 10 days after transplanting (DDT), while plant height and number of leaves were evaluated at 30 and 90 DDT. For the variables height and number of leaves, an analysis of variance between the treatments (substrates) and additionally an orthogonal contrast between the substrates vs. the control (river sand) was carried out. The comparison of means for the variables with statistical significance was carried out with the Duncan test at 5% probability. There were high survival rates in all treatments, and a significant effect of the substrates on the height and number of leaves in comparison with the control treatment. Keywords: Acclimatisation; ex vitro; substrates.

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