Morphofunctional assessment of platelet activity correction in hyperbaric blood oxygenation

100 patients with functional class II-III exertional angina were examined. The subjects underwent a 10-day course of hyperbaric oxygenation (HBO) in the 1.2 ATA mode for 40 minutes, against the background of standard therapy for coronary heart disease (IHD). Before and after HBO, the platelet link of hemostasis and the elastic properties of the platelet membrane were assessed. Assessment of the effect of the HBO course on the functional state of platelets, depending on their aggregation activity, showed that in patients with initially normal platelet aggregation, there is a tendency to a decrease in spontaneous aggregation, in contrast to patients with initial hypoaggregation, HBO promoted a significant increase in spontaneous aggregation. The use of a 10-day course of HBO was accompanied by a reaction of reducing the elasticity of the platelet biomembrane. Key words: aggregation; atomic force microscopy; platelet membrane; hyperbaric oxygenation.

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Platelet activity in patients with hyper- and hypoparathyroidism

Problems of Endocrinology ◽

10.14341/probl11753 ◽

2003 ◽

Vol 49 (5) ◽

pp. 32-38

Author(s):

V. F. Kirichuk ◽

F. G. Arefyev

Keyword(s):

Parathyroid Hormone ◽

Functional Activity ◽

Platelet Rich Plasma ◽

Partial Recovery ◽

Platelet Activity ◽

Aggregation Response ◽

Activated Platelets ◽

Before And After ◽

Platelet Aggregates ◽

Aggregation Activity

Platelet aggregatory activity was studied in a patient with hyperparathyroidism before and after parathyroid adenomectomy and in 3 patients with hypoparathyroidism. In these patients, changes in the aggregation activity of platelet were evaluated after their incubation with parathyroid hormone. The patient with hyperparathyroidism was found to have activated platelets, their high readiness for an aggregation response, increased and a highly effective aggregatory activity and insignificantly decreased stability of formed platelet aggregates. After parathyroid adenomectomy there was a partial recovery ofplatelet functional activity. The patients with hypoparathyroidism had a lower platelet functional activity as compared with the controls. Preincubation of platelet-rich plasma from the patients with hypoparathyroidism with parathyroid hormone caused a varying recovery of platelet functional activity.

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EFFECT OF DDAVP ON PRIMARY HEMOSTASIS WITH CONGENITAL AFIBRINOGENEMIA

10.1055/s-0038-1644129 ◽

1987 ◽

Author(s):

M Taki ◽

M Inagaki ◽

T Miura ◽

N Saito ◽

T Meguro ◽

...

Keyword(s):

Platelet Aggregation ◽

Retention Rate ◽

Platelet Membrane ◽

Bleeding Tendency ◽

Primary Hemostasis ◽

Prolonged Bleeding Time ◽

Before And After ◽

Congenital Afibrinogenemia ◽

Von Willebrand ◽

Platelet Functions

It has been reported recently that DDAVP might be an useful tool in the therapy and prevention of bleeding in patients with congenital afibrinogenemia (CA).To study the mechanism of its efficacy, changes in the platelet functions of a patient with CA were examined prior to, and one hour after, the infusion of DDAVP (0.4 μg/Kg). A patient with Glanzmann's thrombasthenia (GT) was also examined, to allow a study of the role of platelet membrane glycoprotein IIb/IIIa (GP IIb/IIIa), a deficient platelet in GT, in the resulting effects of the drug. When both patients were infused with DDAVP, the level of plasma von Willebrand factor (vWF) increased two- to fourfold, accompanied by an enhancement of ristocetin-induced platelet agglutination. The level of plasma fibrinogen was never changed.The prolonged bleeding time observed was markedly improved only in the CA patient, remaining unchanged in the GT patient, after the infusion of DDAVP. This indicates that DDAVP is effective in diminishing the bleeding tendency in CA, but not in GT. Among the platelet functions tested, only the platelet retention rate on glass beads, ADP-induced platelet aggregation and collagen-induced platelet aggregation improved in CA, each remaining unchanged in GT. In particular, collagen-induced platelet aggregation was markedly improved in the CA patient. However, the platelet adhesion to collagen (50 μg/ml)-Sepharose remained normal, both before and after the infusion of DDAVP in CA.These results suggest that an increase in the plasma vWF level and the existence of platelet membrane GPIIb/IIIa may be necessary for the improvement of primary hemostasis, after the infusion of DDAVP. The vWF-mediated platelet aggregation by collagen or ADP may produce this effect in the CA patient.

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Registration of Prehospital Vital Parameters

Dansk Tidsskrift for Akutmedicin ◽

10.7146/akut.v2i3.112991 ◽

2019 ◽

Vol 2 (3) ◽

pp. 49

Author(s):

Ninna Rysholt Poulsen

Keyword(s):

New Technologies ◽

Rating Scale ◽

Implementation Process ◽

Numeric Rating Scale ◽

Blood Oxygenation ◽

Cut Points ◽

The North ◽

Normal Ranges ◽

Before And After ◽

Vital Parameters

Background: The North Denmark Region implemented an electronic Prehospital Patient medical Record (PPR) in 2006. In 2015 a new version of the PPR was implemented. Implementation of new technologies can be challenging, including structurally and organizational obstacles, which causes difficulty in achieving data completeness. The aim was to examine completeness of registrations of vital parameters in PPR before and after the new version, and examine the distribution of the registrations. Method: The cohort includes all patients to whom an ambulance was dispatched after an emergency 112-call in the North Denmark Region from 2007-2014 and 2016. We examined the registration and distribution of six vital parameters, and included the first measurement. A trend analysis was used to assess the change in registration from 2007-2014, 2016. To exclude outliers in registrations we defined cut-points for systolic blood pressure (BP) (250mmHG), heart rate (HR) (250 beats per minute), and Respiratory Rate (RR) (100 breaths per minute), based on clinical relevance and their natural distributions. The other vital parameters had well-defined cut-points. We examined the distributions with and without outliers. Results: We identified 220,173 patients. Percentage of registrations without outliers from 2007 to 2014 vs. 2016: BP 73% to 86% vs. 81%, HR 76% to 88% vs. 81%, blood oxygenation (SpO2) 72% to 85% vs. 81%, RR 34% to 82% vs. 77%, Glasgow-Coma-Scale-score (GCS) 54% to 92% vs. 81%, Numeric Rating Scale for pain (NRS) 0% to 16% vs. 24%. Data from all years showed normal distributions for systolic BP and HR with mean (95% confidence interval): 141.8 (141.7; 142), 91.9 (91.8; 92.1), respectively. For RR, SpO2 and NRS; median (interquartile range) was: 18 (16; 20), 98 (95; 99), 6 (3; 8). From 2007-2014, 82% of the GCS-scores were on 15; in 2016 85% were on 15. There was 51% of the patients who had five vital parameters measured from 2007-2014 and 47% in 2016. Conclusion: Registration increased significantly from 2007 to 2014, with a significant decrease in 2016, except for the NRS-score. The decrease in 2016 is probably attributed the implementation process. Overall vital parameters were within normal ranges, despite outliers in registration.

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Effect of parameters of renal replacement therapy on platelet aggregation activity

Тромбоз, гемостаз и реология ◽

10.25555/thr.2020.2.0922 ◽

2020 ◽

Author(s):

Т.В. Марченко ◽

А.В. Гончарова ◽

И.Н. Соловьева ◽

Е.О. Марченко ◽

А.М. Исаева

Keyword(s):

Chronic Kidney Disease ◽

Platelet Aggregation ◽

Renal Replacement Therapy ◽

Kidney Disease ◽

Replacement Therapy ◽

Normal Range ◽

Dialysis Therapy ◽

Before And After ◽

Aggregation Activity

Цель исследования: оценить влияние параметров заместительной почечной терапии (ЗПТ) на агрегационную активность тромбоцитов у пациентов с хронической болезнью почек (ХБН). Материалы и методы. Было выполнено 25 процедур гемодиализа (ГД) и 10 процедур гемодиафильтрации (ГДФ) 35 больным с ХБП. Изучали динамику агрегационной активности тромбоцитов до и после экстракорпоральных процедур. Результаты. После процедуры ГД агрегация тромбоцитов снижалась, а после процедуры ГДФ нарастала, не выходя за пределы нормальных значений. Параметры процедур ЗПТ на агрегацию тромбоцитов значимого влияния не оказывали. Заключение. Разовая процедура ЗПТ протяженностью не более 4 ч, проводимая с учетом всех современных требований к диализной терапии, не оказывает негативного влияния на функциональную активность тромбоцитов. Процедура ГДФ приводит к непринципиальному росту агрегационной активности тромбоцитов. Aim: to assess the effect of renal replacement therapy (RRT) parameters on platelet aggregation activity in patients with chronic kidney disease (CKD). Materials and methods. For 35 patients with CKD 25 hemodialysis (HD) procedures and 10 hemodiafiltration (HDF) procedures were performed. We studied the dynamics of platelet aggregation activity before and after extracorporeal procedures. Results. After the HD procedure, platelet aggregation decreased, and after the HDF procedure it increased, without going outside the normal range. Parameters of RRT procedures did not have a significant effect on platelet aggregation. Conclusion. A single RRT procedure not more than 4 hours with all nowadays requirements for dialysis therapy does not adversely affect the functional platelets activity. The HDF procedure leads to an unprincipled increasing of platelet aggregation activity.

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Real-Time Three-Dimensional Echocardiographic Study of Left Ventricular Function After Infarct Exclusion Surgery for Ischemic Cardiomyopathy

Circulation ◽

10.1161/circ.102.suppl_3.iii-101 ◽

2000 ◽

Vol 102 (suppl_3) ◽

Author(s):

Jian Xin Qin ◽

Takahiro Shiota ◽

Patrick M. McCarthy ◽

Michael S. Firstenberg ◽

Neil L. Greenberg ◽

...

Keyword(s):

New York ◽

Ischemic Cardiomyopathy ◽

Heart Association ◽

Left Ventricular ◽

Functional Class ◽

Before And After ◽

Infarct Exclusion ◽

New York Heart Association ◽

Lv Volume

Background —Infarct exclusion (IE) surgery, a technique of left ventricular (LV) reconstruction for dyskinetic or akinetic LV segments in patients with ischemic cardiomyopathy, requires accurate volume quantification to determine the impact of surgery due to complicated geometric changes. Methods and Results —Thirty patients who underwent IE (mean age 61±8 years, 73% men) had epicardial real-time 3-dimensional echocardiographic (RT3DE) studies performed before and after IE. RT3DE follow-up was performed transthoracically 42±67 days after surgery in 22 patients. Repeated measures ANOVA was used to compare the values before and after IE surgery and at follow-up. Significant decreases in LV end-diastolic (EDVI) and end-systolic (ESVI) volume indices were apparent immediately after IE and in follow-up (EDVI 99±40, 67±26, and 71±31 mL/m 2 , respectively; ESVI 72±37, 40±21, and 42±22 mL/m 2 , respectively; P <0.05). LV ejection fraction increased significantly and remained higher (0.29±0.11, 0.43±0.13, and 0.42±0.09, respectively, P <0.05). Forward stroke volume in 16 patients with preoperative mitral regurgitation significantly improved after IE and in follow-up (22±12, 53±24, and 58±21 mL, respectively, P <0.005). New York Heart Association functional class at an average 285±144 days of clinical follow-up significantly improved from 3.0±0.8 to 1.8±0.8 ( P <0.0001). Smaller end-diastolic and end-systolic volumes measured with RT3DE immediately after IE were closely related to improvement in New York Heart Association functional class at clinical follow-up (Spearman’s ρ=0.58 and 0.60, respectively). Conclusions —RT3DE can be used to quantitatively assess changes in LV volume and function after complicated LV reconstruction. Decreased LV volume and increased ejection fraction imply a reduction in LV wall stress after IE surgery and are predictive of symptomatic improvement.

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Functional activity of platelets in aged patients with chronic heart failure with hyperbaric oxygenation

10.34014/mpphe.2021-6-8 ◽

2021 ◽

Author(s):

E.N. Altynbaeva ◽

V.I. Ruzov ◽

M.N. Goryachaya ◽

N.A. Slobodnjuk

Keyword(s):

Heart Failure ◽

Chronic Heart Failure ◽

Functional Activity ◽

Hyperbaric Oxygenation ◽

Functional Class ◽

Hyperbaric Oxygen Treatment ◽

Initial State ◽

Aged Patients ◽

Oxygen Treatment ◽

Induced Aggregation

105 patients with chronic heart failure (CHF) with ischemic etiology of functional class were examined, among them 70 men (67%) and 35 women (33%), average age 78,67±13,80 years. A five-day course of hyperbaric oxygen treatment in the BLKS-303MK pressure chamber (Russia) in the 1.2 ATA with a 30-45 minutes session was accompanied by a study of the func-tional activity of platelets. It was found that after hyperbaric oxygenation treatment ADP-induced aggregation significantly decreased in women, spontaneous aggregation in men. How-ever, the nature of the response of platelet functional activity to hyperbaric oxygenation is af-fected by the initial state of the platelet hemostasis. Key words: platelets, hyperbaric oxygenation, platelet aggregation, heart failure, hemo-stasis.

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Platelet Counts in Stored Donor Blood

Anaesthesia and Intensive Care ◽

10.1177/0310057x8201000318 ◽

1982 ◽

Vol 10 (3) ◽

pp. 271-273 ◽

Cited By ~ 4

Author(s):

W. J. Russell ◽

L. J. Tunbridge

Keyword(s):

Blood Platelet ◽

Platelet Activity ◽

Normal Range ◽

Donor Blood ◽

Platelet Counts ◽

Before And After ◽

The Mean ◽

Clinically Significant ◽

The One ◽

Citrate Phosphate

Platelets were counted in eight units each of one- and three-day-old blood. Counts were done both before and after the blood had passed through a standard 170 micron filter. In the one-day-old blood, platelet counts were within the normal range. The mean count was 237,000 platelets/microlitre. Platelet counts on three-day-old blood were lower, but generally still within the normal range. The mean count was 183,000/microlitre. Only a few platelets were retained by the filter in the transfusion set; about 90% of platelets passed the filter in both the one- and three-day-old blood. It appears that whole blood, anticoagulated with citrate/phosphate/dextrose (CPD), and stored under Australian blood bank conditions retains platelets in sufficient numbers for at least the first three days to be clinically significant. However, it remains to be determined whether satisfactory platelet activity can be expected during this time.

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IN VITRO ANTI-PLATELET AGGREGATION ACTIVITY OF HYDROXYPROPYL CELLULOSE–CYSTEAMINE BASED NANOPARTICLES CONTAINING CRUDE BROMELAIN

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020v12i3.35053 ◽

2020 ◽

pp. 95-98

Author(s):

DENI RAHMAT ◽

LILIEK NURHIDAYATI ◽

MARCELLA MARCELLA ◽

ROS SUMARNY ◽

DIAN RATIH LAKSMITAWATI

Keyword(s):

Particle Size ◽

Platelet Aggregation ◽

Zeta Potential ◽

Light Transmission ◽

Hydroxypropyl Cellulose ◽

Platelet Activity ◽

Two Samples ◽

Significant Difference ◽

Aggregation Activity

Objective: The aim of the present study was to formulate bromelain into nanoparticles in order to improve its stability and activity. Methods: Crude bromelain was prepared by protein precipitation from the pineapple stem juice using ammonium sulphate at the concentration of 60% (w/v). Nanoparticles containing crude bromelain were generated using the ionic gelation method with hydroxypropyl cellulose–cysteamine (HPC-cysteamine) conjugate as a matrix. Crude bromelain was then added to the HPC-cysteamine solution for ionic interaction to construct the nanoparticles, which were then analyzed for their particle size and zeta potential. The resulting nanoparticles were mixed with adenosine diphosphate (ADP) to perform anti-platelet aggregation. Results: The nanoparticle had 928.3 nm in particle size and-7.25 mV in zeta potential. Anti-platelet activity of crude bromelain and the nanoparticles were determined with modification of light transmission aggregometry (LTA), in which ADP was used to induce an aggregation while a spectrophotometer UV-Vis was used to measure the absorbance at the wavelength of 600 nm. The result showed that crude bromelain and the nanoparticles rendered percentage inhibition of 8.00±1.17% and 48.56±11.19%, respectively. Conclusion: Based on the result of a one-way analysis of variance (ANOVA), it was concluded that there was a significant difference in percentage inhibition between the two samples. The nanoparticles demonstrated a better anti-platelet aggregation activity compared to crude bromelain.

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