In vitrogrowth behaviour of probiotic bacteria in culture media with carbohydrates of prebiotic importance

2000 ◽  
Vol 12 (1) ◽  
Author(s):  
Wolfgang Kneifel ◽  
Andreas Rajal ◽  
Klaus Dieter Kulbe
Keyword(s):  
Culture Media ◽  
Probiotic Bacteria

scholarly journals STUDI BACILLUS FIRMUS SEBAGAI KANDIDAT PROBIOTIK DALAM MENGHADAPI Aeromonas hydrophila PADA MEDIA BUDIDAYA The Study of Bacillus firmus as Probiotic Candidate in Supressing Aeromonas hydrophila in Culture Media

2016 ◽  
Vol 11 (2) ◽  
pp. 111
Author(s):  
Alfabetian Harjuno Condro Haditomo ◽  
Angela Mariana Lusiastuti ◽  
Widanarni Widanarni
Keyword(s):  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Inhibition Zone ◽  
Probiotic Bacteria ◽  
Bacterial Disease ◽  
Second Phase ◽  
Bacillus Firmus ◽  
Culture Methods

ABSTRAK   Pengendalian penyakit bakterial yang umum dilakukan dengan pemakaian antibiotik atau  bahan kimia sudah tidak diperbolehkan lagi karena menimbulkan patogen yang resisten  terhadap bahan kimia tersebut, terlebih jika penggunaan tidak sesuai dengan anjuran yang diberikan. Dampak negatif terhadap kesehatan konsumen berupa residu antibiotik juga menjadi pertimbangan yang harus diperhatikan. Manipulasi terhadap populasi mikroba yang berada di perairan guna pencegahan sebelum terjadinya serangan bakteri yang bersifat mematikan perlu dilakukan sebagaimana konsep probiotik sebagai biokontrol. Tujuan penelitian ini adalah menguji kandidat probiotik dalam menekan atau menghambat bakteri patogen Aeromonas hydrophila. Penelitian ini dilaksananakan dalam dua tahap. Tahap pertama adalah tahap pengujian bakteri kandidat probiotik secara in vitro menggunakan metode zona hambat dan kultur bersama pada media agar.  Tahap kedua adalah uji tentang bakteri kandidat probiotik dengan patogen pada media budidaya. Hasil terbaik penelitian tahap pertama pada  uji kultur bersama antara kandidat probiotik B. firmus dengan A. hydrophila pada skala in vitro adalah dengan penambahan probiotik  B. firmus sebanyak 108 cfu/ml. Sedangkan pada penelitian tahap kedua didapatkan hasil berturut-turut perlakuan D dengan tingkat kelangsungan hidup (SR) mencapai 90%, perlakuan C dengan SR 75%, perlakuan A dengan SR 50% dan perlakuan K dengan SR 50%.   Kata kunci: Bacillus firmus, probiotik, Aeromonas hydrophila, media budidaya   ABSTRACT  Controlling bacterial disease with the use of antibiotics or chemicals is no longer allowed as it results in pathogens that are resistant to the chemicals, especially when not in accordance with the recommendations provided. The negative impactsof the antibiotics residues on the consumers’ health  also need to be considered. Manipulation of microbial populations present in the waters as preventation before the lethal attack of bacteria needs to be done which is in accordance with the concept of probiotics as biocontrol.The purpose of this study was to test the probiotic candidates in suppressing or inhibiting pathogenic bacteria Aeromonas hydrophila. This study was conducted in two stages. The first stage was to test a candidate probiotic bacteria in vitro using culture methods and inhibition zone on the media together. The second stage wasto test candidate probiotic bacteria to pathogens on the cultivation media. The best results in the first phase of the research is shared culture test between probiotic candidate B. FIRMUS with A. hydrophila on vitro scale is the addition of the probiotic B. FIRMUS 108 cfu / ml. While in the second phase of the research results obtained successively: treatment D with a survival rate (SR) reaches 90%, treatment C with SR 75%, treatment A with SR 50% and treatment K with SR 50%. Keywords: Bacillus FIRMUS, probiotics, Aeromonas hydrophila, media cultivation

scholarly journals Evolutionary changes of an intestinalLactobacillus reuteriduring probiotic manufacture

2019 ◽  
Author(s):  
Lázaro López ◽  
Paúl Cardenas ◽  
María B. Prado ◽  
Gabriel Trueba
Keyword(s):  
Microbial Community ◽  
Culture Medium ◽  
Lactobacillus Reuteri ◽  
Culture Media ◽  
Probiotic Bacteria ◽  
Synonymous Mutations ◽  
Metabolic Genes ◽  
Evolutionary Changes ◽  
Mammalian Intestine ◽  
Improve Health

ABSTRACTProbiotic bacteria are frequently used to treat intestinal (and other types of diseases) or to improve health, however little is known about the evolutionary changes of these bacteria during probiotic manufacture. It has been observed that when bacteria adapt to a new environment, they lose the skills to thrive in the original niche. In this study, a strain ofLactobacillus reuteriwas isolated from the duodenum of a mouse and subjected to 150 serial passes in milk to simulate industrial propagation of probiotic bacteria. The strain adapted to milk outperformed its ancestor in milk, but it showed reduced aptitude to grow culture media, and possibly in mouse intestines. Bacterial adaptation to milk seemed to select a number of non-synonymous mutations in metabolic genes.IMPORTANCEBacteria in the microbiota must endure hostile conditions including antagonism from other members of the microbial community. Little is known about the evolution of probiotic bacteria in the dairy industry. We show that repeated growth ofL. reuteriin milk results in reduced ability to grow in culture medium and possibly colonize the mammalian intestine. Our results contribute to the notion that probiotic bacteria colonize only transiently intestines.

scholarly journals Effect of Sucrose as Carbon Source and Probiotic Administrations on Bacterial Population Dinamic and Water Quality in White Shrimp, Litopenaeus vannamei Culture

2007 ◽  
Vol 5 (2) ◽  
pp. 179
Author(s):  
. Sukenda ◽  
P. Hadi ◽  
E. Harris
Keyword(s):  
Water Quality ◽  
Carbon Source ◽  
Litopenaeus Vannamei ◽  
Bacterial Population ◽  
Negative Impact ◽  
Culture Media ◽  
Probiotic Bacteria ◽  
White Shrimp ◽  
Shrimp Culture ◽  
Commercial Diet

<p>Disinfection and nutrient enrichment prior stocking of  post larvae in the pond will be affected on the growth and composition of microbe.  Attention should be taken to some factors related to deterministic and stochastic factors of aquaculture environment  in order to develop microbe community.  This study was performed to determine effect of sucrose and probiotic supplementation to shrimp culture pond on water quality profile and population dynamic on shrimp culture media.  The treatments were supplementation of sucrose as carbon source, probiotic, and sucrose + probiotic into 25 L culture medium containing white shrimp, <em>Litopenaeus vannamei</em>.  Shrimp were fed commercial diet containing 30% protein by 5% body weight every day.  The result of study showed that bacterial population was increased by increasing time of shrimp rearing.  Increased of bacterial population was contrary to DO value.   Bacteria grew was heterotrop and vibrio that its intensity varied during experiment.  Supplementation of sucrose supported proliferation of bacteria including heterotrop, probiotik and vibrio groups.  Specifically, interaction between probiotic bacteria and vibrio was also found.  The presence of probiotic bacteria showed a negative impact on vibrio population.  Further, development of bacteria in general was also implicated to fluctuation of ammonia concentration in pond.</p> <p>Keywords: carbon, sucrose, probiotic, white shrimp, <em>Litopenaeus vannamei</em></p> <p> </p> <p>ABSTRAK</p> <p>Kegiatan disinfeksi dan pengkayaan nutrien sebelum penebaran PL akan mempengaruhi pola pertumbuhan dan komposisi mikroba di tambak. Hal-hal yang perlu diperhatikan dalam pengembangan komunitas mikroba adalah faktor-faktor <em>deterministic</em> dan <em>sthocastic</em> masing-masing lingkungan budidaya. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian bahan berkarbon (sukrosa) dan probiotik di tambak terhadap profil kualitas air serta dinamika populasi pada perairan budidaya. Pada penelitian ini dilakukan penambahan sumber karbon (sukrosa), penambahan probiotik dan penambahan sukrosa + probiotik pada masing-masing wadah yang berisi 25 liter air dan udang Vanamei, <em>Litopenaeus vannamei</em>. Pakan yang diberikan berupa pellet komersial dengan kadar protein 30% setiap hari sebanyak 5% dari biomassa awal. Dari hasil penelitian ini diketahui bahwa populasi bakteri pada media budidaya meningkat seiring dengan bertambahnya waktu pemeliharaan. Peningkatan jumlah populasi bakteri ini diikuti oleh semakin menurunnya nilai DO secara umum. Selain bakteri heterohof, tumbuh juga bakteri golongan <em>Vibrio</em> dengan persentase kemunculan yang berbeda pada setiap perlakuan. Penambahan sukrosa dalam media budidaya mendukung proliferasi bakteri secara umum, heterotrof, probiotik dan golongan <em>Vibrio</em>. Secara spesifik, timbul juga interaksi antara bakteri probiotik dengan bakteri <em>Vibrio</em>. Tumbuhnya bakteri probiotik berimplikasi negatif terhadap populasi <em>V</em><em>i</em><em>brio</em>. Selain itu pertumbuhan bakteri secara umum juga berimplikasi terhadap fluktuasi kadar amonia perairan.</p> <p>Kata kunci : karbon, sukrosa, probiotik, udang vaname, <em>Litopenaeus vannamei</em></p>

scholarly journals Production of Marine Probiotic Bacteria in a Cost-Effective Marine Media Based on Peptones Obtained from Discarded Fish By-Products

2020 ◽  
Vol 8 (8) ◽  
pp. 1121 ◽  
Author(s):  
José Antonio Vázquez ◽  
Ana Durán ◽  
Margarita Nogueira ◽  
Araceli Menduíña ◽  
Joana Antunes ◽  
...  
Keyword(s):  
Marine Bacteria ◽  
Culture Media ◽  
Low Cost ◽  
Nitrogen Sources ◽  
Cost Effective ◽  
Probiotic Bacteria ◽  
Probiotic Properties ◽  
Economical Evaluation ◽  
The Cost ◽  
By Products

The industrial production of marine bacteria with probiotic properties is limited by the excessive cost of the culture media adequate for their growth. The present work aimed to study the suitability of 30 marine media formulated with nitrogen sources (fish peptones) from different fish discards and seawater, for the growth of two marine probiotic bacteria (MPB), namely Phaeobacter sp. and Pseudomonas fluorescens. These fish peptones were produced from several discarded fish and by-products (heads, skins, and whole individuals of megrim, mackerel, gurnard, hake, etc.). In all cultivations, no significant differences were found on cell viability when the microorganism grew on commercial or alternative media. In relation to the biomass production, the growth of Phaeobacter sp. on waste media was commonly similar or a 20% lower than observed in the control cultures. For P. fluorescens, various peptones (skin peptones of pouting and blue whiting) showed even higher productive ability than commercial peptones. An initial economical evaluation revealed that low-cost media reduced until 120 times the cost of production of MPB.

scholarly journals Cholesterol Assimilation byLactobacillusProbiotic Bacteria: AnIn VitroInvestigation

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Catherine Tomaro-Duchesneau ◽  
Mitchell L. Jones ◽  
Divya Shah ◽  
Poonam Jain ◽  
Shyamali Saha ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
Culture Media ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Bacteria ◽  
Bile Salt Hydrolase ◽  
Dietary Interventions ◽  
Cholesterol Levels ◽  
Probiotic Strains ◽  
Therapeutic Measures ◽  
Pharmaceutical Agents

Excess cholesterol is associated with cardiovascular diseases (CVD), an important cause of mortality worldwide. Current CVD therapeutic measures, lifestyle and dietary interventions, and pharmaceutical agents for regulating cholesterol levels are inadequate. Probiotic bacteria have demonstrated potential to lower cholesterol levels by different mechanisms, including bile salt hydrolase activity, production of compounds that inhibit enzymes such as 3-hydroxy-3-methylglutaryl coenzyme A, and cholesterol assimilation. This work investigates 11Lactobacillusstrains for cholesterol assimilation. Probiotic strains for investigation were selected from the literature:Lactobacillus reuteriNCIMB 11951,L. reuteriNCIMB 701359,L. reuteriNCIMB 702655,L. reuteriNCIMB 701089,L. reuteriNCIMB 702656,Lactobacillus fermentumNCIMB 5221,L. fermentumNCIMB 8829,L. fermentumNCIMB 2797,Lactobacillus rhamnosusATCC 53103 GG,Lactobacillus acidophilusATCC 314, andLactobacillus plantarumATCC 14917. Cholesterol assimilation was investigated in culture media and under simulated intestinal conditions. The best cholesterol assimilator wasL. plantarumATCC 14917 (15.18 ± 0.55 mg/1010 cfu) in MRS broth.L. reuteriNCIMB 701089 assimilated over 67% (2254.70 ± 63.33 mg/1010 cfu) of cholesterol, the most of all the strains, under intestinal conditions. This work demonstrates that probiotic bacteria can assimilate cholesterol under intestinal conditions, withL. reuteriNCIMB 701089 showing great potential as a CVD therapeutic.

The effect of probiotic bacteria in culture media using organic fertilizer for population density, biomass production and nutrient quality of Phronima sp . as natural feed

2019 ◽  
Vol 51 (2) ◽  
pp. 836-843 ◽  
Author(s):  
Vivi Endar Herawati ◽  
P. Pinandoyo ◽  
Nurmanita Rismaningsih ◽  
D. Darmanto ◽  
Johannes Hutabarat ◽  
...  
Keyword(s):  
Population Density ◽  
Biomass Production ◽  
Culture Media ◽  
Organic Fertilizer ◽  
Probiotic Bacteria ◽  
Nutrient Quality

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

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