Faculty Opinions recommendation of Neuropixels 2.0: A miniaturized high-density probe for stable, long-term brain recordings.

Objective: This study was designed to analyze the clinical effect of autologous fat-granule transplantation in augmentation rhinoplasty and explore methods to improve the fat retention rate. Methods: A total of 70 enrolled patients were randomly divided into 2 groups: the platelet-rich fibrin (PRF) combined with high-density fat transplantation group (combined group) and the conventional fat-granule transplantation group (control group; n = 35 in each group). In the combined group, an appropriate amount of autologous fat was extracted and centrifuged, and the lower layer of high-density fat was taken and mixed with PRF isolated from whole blood for autotransplantation. In the control group, only fat was extracted and centrifuged for transplantation. The patients were followed up with for more than one year to observe the short- and long-term effects, complications, safety, and patient satisfaction. Results: Six months after the operation, the nasal shape was stable, the contour was higher and more stereoscopic than before, the average increase of nasal height was 3.0 mm in the combined group and 2.0 mm in the control group. No complications, such as fat embolism, infection, or necrosis occurred during the 1-year follow-up. The satisfaction rate between the 2 groups has statistical significance ( P < .05). Conclusion: Overall, PRF combined with autologous high-density fat transplantation is simple to perform, has a significantly increased fat-retention rate than the control group, and has stable long-term effects without obvious adverse reactions. A sufficient amount of fat and PRF transplantation can achieve a good orthopedic effect. Thus, this method can be widely used in clinical augmentation rhinoplasty.

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Evaluation of long-term frozen storage of plasma for measurement of high-density lipoprotein and its subfractions by precipitation

Clinical Chemistry ◽

10.1093/clinchem/36.5.783 ◽

1990 ◽

Vol 36 (5) ◽

pp. 783-788 ◽

Cited By ~ 16

Author(s):

M N Nanjee ◽

N E Miller

Keyword(s):

High Density Lipoprotein ◽

Liquid Nitrogen ◽

Frozen Storage ◽

Density Lipoprotein ◽

High Density ◽

Long Term Stability ◽

Long Term Storage ◽

Different Temperatures ◽

Edta Plasma

Abstract The concentration of high-density lipoprotein cholesterol (HDL-C) in plasma is now established as an independent risk factor for coronary heart disease, but more data are needed on the relative risk-predictive powers of different HDL subclasses. For epidemiologic and clinical purposes, isolation of HDL from other lipoproteins and separation of its two major subclasses, HDL2 and HDL3, are performed most conveniently by precipitation. Although storage of plasma is commonly necessary, little information is available on the long-term stability of HDL subclasses at different temperatures. Therefore, we quantified HDL-C, HDL2-C, and HDL3-C by dual precipitation with heparin-MnCl2/15-kDa dextran sulfate (H-M/DS) in samples of EDTA-plasma from 93 healthy subjects, after storage for one to 433 days at -20 degrees C, at -70 degrees C, or in liquid nitrogen (-196 degrees C). Fourteen samples (15%) were stored for a year or longer. At -20 degrees C, HDL-C decreased by 4.8% per year and HDL3-C decreased by 6.9% per year (P = 0.002 for both variables) relative to results obtained with samples stored in liquid nitrogen; total cholesterol, HDL2-C, and triglyceride did not change significantly at this temperature. When stored at -70 degrees C, none of the lipids showed any change relative to results obtained with liquid nitrogen. Thus, long-term storage of EDTA-plasma at -20 degrees C is unsuitable for subsequent quantification of HDL-C and its subclasses by H-M/DS dual precipitation. Storage at -70 degrees C is preferable, and is as reliable as storage in liquid nitrogen.

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The short- and long-term compressive behavior of high-density polyethylene geonet and geocomposite under inclined conditions

Geosynthetics International ◽

10.1680/gein.2007.14.3.154 ◽

2007 ◽

Vol 14 (3) ◽

pp. 154-164 ◽

Cited By ~ 5

Author(s):

S.-S. Yeo ◽

Y. G. Hsuan

Keyword(s):

High Density Polyethylene ◽

High Density ◽

Compressive Behavior ◽

Short And Long Term

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Methodology for Long-Term Permeation Test Periods for HD in High-Density Polyethylene: Universal Munitions Storage Container for the Non-Stockpile Chemical Materiel Program

10.21236/ad1009746 ◽

2016 ◽

Cited By ~ 1

Author(s):

Wendel Shuely ◽

Patricia Boone ◽

Juan Cajigas ◽

Michael Sheely

Keyword(s):

High Density Polyethylene ◽

High Density ◽

Storage Container ◽

Permeation Test

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P3778Long-term outcome of non-pharmacologial treatment of atrial fibrillation in hypertrophic cardiomyopathy: a large single-centre experience

European Heart Journal ◽

10.1093/eurheartj/ehz745.0627 ◽

2019 ◽

Vol 40 (Supplement_1) ◽

Author(s):

L Dinshaw ◽

M Lemoine ◽

J Hartmann ◽

B Schaeffer ◽

N Klatt ◽

...

Keyword(s):

Atrial Fibrillation ◽

Catheter Ablation ◽

Left Atrial ◽

High Density ◽

Term Outcome ◽

Long Term Outcome ◽

Density Mapping ◽

Ablation Success

Abstract Introduction Atrial fibrillation (AF) is common in hypertrophic cardiomyopathy (HCM) and is generally associated with a significant deterioration of clinical status. Non-pharmacological treatment such as surgical and catheter ablation has become an established therapy for symptomatic AF but in patients with HCM often having a chronically increased left atrial pressure and extensive atrial cardiomyopathy the long-term outcome is uncertain. Purpose The present study aimed to analyse the long-term outcome of AF ablation in HCM and the mechanism of recurrent atrial arrhythmias using high-density mapping systems. Methods A total of 65 patients (age 64.5±9.9 years, 42 (64.6%) male) with HCM undergoing AF ablation for symptomatic AF were included in our study. The ablation strategy for catheter ablation included pulmonary vein isolation in all patients and biatrial ablation of complex fractionated electrograms with additional ablation lines if appropriate. In patients with suspected atrial tachycardia (AT) high-density activation and substrate mapping were performed. A surgical ablation at the time of an operative myectomy for left ventricular outflow tract obstruction was performed in 8 (12.3%) patients. The outcome was analysed using clinical assessment, Holter ECG and continuous rhythm monitoring of cardiac implantable electric devices. Results Paroxysmal AF was present in 27 (41.6%), persistent AF in 37 (56.9%) and primary AT in 1 (1.5%) patients. The mean left atrial diameter was 54.1±12.5 ml. In 11 (16.9%) patients with AT high-density mapping was used to characterize the mechanism of the ongoing tachycardia. After 1.9±1.2 ablation procedures and a follow-up of 48.5±37.2 months, ablation success was demonstrated in 58.9% of patients. The success rate for paroxysmal and persistent AF was 70.0% and 55.8%, respectively (p=0.023). Of those patients with AT high-density mapping guided ablation was successful in 44.4% of patients. The LA diameter of patients with a successful ablation was smaller (52.2 vs. 58.1 mm; p=0.003). Conclusion Non-pharmacological treatment of AF in HCM is effective during long-term follow-up. Paroxysmal AF and a smaller LA diameter are favourable for successful ablation. In patients with complex AT the use of high-density mapping can guide ablation resulting in further ablation success in a reasonable number of patients.

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P1377Substrate-based ablation in patients with frequent appropriate ICD therapy and dilated cardiomyopathy: long-term experience with high-density mapping

EP Europace ◽

10.1093/europace/euaa162.204 ◽

2020 ◽

Vol 22 (Supplement_1) ◽

Author(s):

M M O Oliveira ◽

P S C Cunha ◽

B V Valente ◽

G Portugal ◽

A Lousinha ◽

...

Keyword(s):

Left Ventricular ◽

High Density ◽

Border Zone ◽

Long Term Results ◽

Icd Therapy ◽

Electroanatomic Mapping System ◽

Density Mapping ◽

Substrate Modification ◽

Lv Dysfunction

Abstract Recurrent ventricular tachydisrhythmias (VT) episodes have a negative impact in the outcome of patients (P) already with an implantable cardioverter-defibrillator (ICD). Elimination of arrhythmic reentry circuits represents a difficult challenge, mainly due to the induction of intolerable VTs, with multiple ECG morphologies, requiring rapid interruption. Substrate guided ablation has been used as a promising approach strategy to treat recurrent VTs. Aim: to assess long-term results of a VT substrate-based ablation using high-density mapping in P with an ICD, severe left ventricular (LV) dysfunction and recurrent appropriate ICD therapy. Methods: 16P (12 men, non-ischemic cardiomyopathy 67%, 55 ± 13 years, LV ejection fraction 32 ± 6%) and recurrent appropriate shocks despite antiarrhythmic drug therapy and optimal heart failure medication. All P underwent a protocol of ventricular programmed stimulation (600 ms/S3) to obtain baseline VT documentation. A sinus rhythm (SR) voltage map was created using a 3D electroanatomic mapping system (CARTO) with a high-density mapping catheter (PentaRay) to delineate areas of scarred myocardium (ventricular bipolar voltage ≤0,5 mV – dense scar; 0,5-1,5 mV – border zone; ≥1,5 mV – healthy tissue) and provide high-resolution electrophysiological mapping. The substrate modification included catheter elimination of local abnormal ventricular activities (LAVA) - fractionated, splited, low-amplitude/long-lasting, late potentials, pre-systolic potentials - and linear ablation to obtain scars homogenization and scar dechanneling. Pace-mapping techniques were used when capture was possible. LV approach was retrograde in 6 cases, transeptal in 4 and endo-epicardial in 2 cases. In 2P the ablation was performed in the right ventricle. Results: VTs were induced and interrupted with bursts or external DC shocks. LAVA were identified and ablated in all P. Eleven P underwent modification of scar areas. The mean duration of the procedure was 153 mn (103-218 mn), with radiofrequency ranging from 18 to 60 mn (mean 33 min), and a mean fluoroscopy time of 16 mn. Non-inducibility was achieved in 75% of the cases. There was 1 pericardial tamponade drained successfully. During a follow-up of 48 ± 18 months, 75% had no VT recurrences, 2P underwent redo ablation, 1P died from stroke. Conclusion: Catheter ablation of VT based on substrate modification guided by high-density mapping is feasible and safe in P with LV dysfunction. This approach may be of clinical relevance, with potential benefits in reducing VT burden.

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Effects of overstory competition on canopy recruitment patterns of naturally regenerated longleaf pine on two site types

Canadian Journal of Forest Research ◽

10.1139/cjfr-2019-0343 ◽

2020 ◽

Vol 50 (7) ◽

pp. 624-635

Author(s):

Patrick J. Curtin ◽

Benjamin O. Knapp ◽

Steven B. Jack ◽

Lance A. Vickers ◽

David R. Larsen ◽

...

Keyword(s):

Longleaf Pine ◽

Pinus Palustris ◽

Height Growth ◽

Growth Patterns ◽

High Density ◽

Low Density ◽

Individual Tree ◽

Pine Trees ◽

Overstory Trees

Recent interest in continuous cover forest management of longleaf pine (Pinus palustris Mill.) ecosystems raises questions of long-term sustainability because of uncertainty in rates of canopy recruitment of longleaf pine trees. We destructively sampled 130 naturally regenerated, midstory longleaf pines across an 11 300 ha, second-growth longleaf pine landscape in southwestern Georgia, United States, to reconstruct individual tree height growth patterns. We tested effects of stand density (using a competition index) and site quality (based on two site classifications: mesic and xeric) on height growth and demographics of midstory trees. We also compared height growth of paired midstory and overstory trees to infer stand regeneration and recruitment dynamics. In low-density stands, midstory trees were younger and grew at greater rates than trees within high-density stands. Midstory trees in low-density stands were mostly from a younger regeneration cohort than their paired overstory trees, whereas midstory–overstory pairs in high-density stands were mostly of the same cohort. Our results highlight the importance of releasing midstory longleaf pine trees from local competition for sustained height growth in partial-harvesting management systems. They also demonstrate patterns of long-term persistence in high-density stands, indicating flexibility in the canopy recruitment process of this shade-intolerant tree species.

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