scholarly journals High-intensity interval training ameliorates high-fat diet-induced elevation of aminotransferases in male Wistar rats

2021 ◽  
Vol 23 (3) ◽  
pp. 111-115
Author(s):  
Qazaleh Asqari ◽  
Farhad Gholami ◽  
Jabbar Bashiri ◽  
Adel Donyaei
Keyword(s):  
High Fat Diet ◽  
Wistar Rats ◽  
Interval Training ◽  
Serum Levels ◽  
Diet Group ◽  
Standard Diet ◽  
High Intensity Interval Training ◽  
High Fat ◽  
Male Wistar Rats ◽  
High Intensity Interval

Background and aims: A high-fat diet increases triglyceride (TG) accumulations in hepatocytes and results in non-alcoholic fatty liver diseases (NAFLDs). In this regard, this study investigated the effect of high-intensity interval training (HIIT), along with a high-fat diet on the serum levels of aminotransferases in male Wistar rats. Methods: Forty male Wistar rats were randomly assigned to the standard diet, high-fat diet, exercise + standard diet, and exercise + high-fat diet groups (each containing 10 animals). HIIT program consisted of 6-12 repetitions of 2-minute highs-intensity exercise (85-90% of the maximum speed) interspersed with 1-minute low-intensity exercise (45-50% peak speed) with the frequency of 5 sessions a week over 12 weeks. High-fat diet groups received a diet regimen including 58% fat, 25% protein, and 17% carbohydrate, ad libitum. The blood samples were taken from the left ventricle 48 hours following the last intervention to assess TG, alanine aminotransferase (ALT), and aspartate amino-transferase (AST) concentrations. Data were analyzed using one-way ANOVA and Tukey’s post-hoc tests. Results: The findings showed the mean of ALT, AST, and TG in the high-fat diet group was significantly greater compared to the standard diet group (P=0.001). Furthermore, the mean of ALT, AST, and TG in the exercise + high-fat diet group was significantly lower in comparison with the high-fat diet group (P=0.01, P=0.017, and P=0.012, respectively). Conclusion: Although HIIT ameliorated high-fat diet-induced elevations in the serum levels of TG, ALT, and AST, they did not reach the baseline levels. Thus, it may indicate that a diet as the underlying cause of NAFLDs is more important than any other interventions such as exercise.

Acute effect of high-intensity interval training exercise on redox status in the ovaries of rats fed a high-fat diet

2021 ◽  
Vol 33 (12) ◽  
pp. 713
Author(s):  
Rodrigo L. Furtado ◽  
Jonathan Elias R. Martins ◽  
Maria Alice F. Oliveira ◽  
Denise D. Guerreiro ◽  
Naiza A. R. de Sá ◽  
...  
Keyword(s):  
High Intensity ◽  
High Fat Diet ◽  
Interval Training ◽  
Redox Status ◽  
Female Rats ◽  
Standard Diet ◽  
High Intensity Interval Training ◽  
High Fat ◽  
Antral Follicles ◽  
High Intensity Interval

This study demonstrates the effect of a single high-intensity interval training (HIIT) session on the redox status of rat ovaries with excess adiposity. Forty Wistar female rats (mean (±s.e.m.) weight 94.40 ± 13.40 g) were divided into two groups and fed either a standard diet (SD) or a high-fat diet (HFD) for 62 days. At the end of this period, the rats were subjected to a single HIIT session and were killed 24 h after exercise. Both groups subjected to exercise (SDex and HFDex) generated a significantly higher antioxidant environment by presenting a higher thiol content, which represents a lower oxidation rate of GSH than their respective controls (SD and HFD). The percentage of morphologically normal primary follicles decreased, whereas that of antral follicles increased, in the SDex group. In addition, the HFD group had a higher percentage of degenerated antral follicles than the SD and SDex groups. Cells immunoreactive for α-smooth muscle actin were seen in the cortical stroma and thecal layer enclosing late secondary and tertiary follicles in all groups. Moreover, heme oxygenase and cytochrome P450 family 19 subfamily A member 1 (Cyp19A1) labelling was seen in all antral follicles. Progesterone concentrations were significantly higher in the HFDex than SDex group. In conclusion, this study indicates that a single session of HIIT may result in an improvement in ovary redox status because of metabolic muscle activity by inducing physiological adaptation after exercise in a paracrine manner.

scholarly journals The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1569
Author(s):  
Marine Dupuit ◽  
Vivien Chavanelle ◽  
Benoit Chassaing ◽  
Fanny Perriere ◽  
Monique Etienne ◽  
...  
Keyword(s):  
Glycemic Control ◽  
High Intensity ◽  
High Fat Diet ◽  
Interval Training ◽  
Standard Diet ◽  
High Intensity Interval Training ◽  
High Fat ◽  
Beneficial Effects ◽  
Α Diversity ◽  
High Intensity Interval

Obesity and prediabetes are the two strongest risk factors of type 2 diabetes. It has been reported that TOTUM-63, a polyphenol-rich plant extract, has beneficial effects on body weight (BW) and insulin resistance in mice fed a high fat diet (HFD). The study aim was to determine whether high-intensity interval training (HIIT) and/or TOTUM-63 supplementation improved body composition and glycemic control and gut microbiota composition in a Western diet-induced obesity rat model. Wistar rats received a standard diet (CTRL; control; n = 12) or HFD (HFD; n = 48) for 16 weeks. Then, HFD rats were divided in four groups: HFD, HFD + TOTUM-63 (T63), HFD + HIIT (HIIT), and HFD + HIIT +T63 (HIIT + T63). Training was performed 4 days/week for 12 weeks. TOTUM-63 was included in diet composition (2%). The HIIT + T63 combination significantly limited BW gain, without any energy intake modulation, and improved glycemic control. BW variation was correlated with increased α-diversity of the colon mucosa microbiota in the HIIT + T63 group. Moreover, the relative abundance of Anaeroplasma, Christensenellaceae and Oscillospira was higher in the HIIT + T63 group. Altogether, these results suggest that the HIIT and TOTUM-63 combination could be proposed for the management of obesity and prediabetes.

scholarly journals High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling

2020 ◽  
Vol Volume 13 ◽  
pp. 165-174 ◽  
Author(s):  
Mehrnoosh Shanaki ◽  
Maryam Khosravi ◽  
Arezoo Khoshdooni-Farahani ◽  
Alireza Dadashi ◽  
Mohammad Foad Heydari ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Notch Signaling ◽  
High Fat Diet ◽  
Macrophage Polarization ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
High Fat ◽  
M1 Macrophage ◽  
High Intensity Interval ◽  
Rat Adipose Tissue

How high-fat diet and high-intensity interval training affect lipid metabolism in the liver and visceral adipose tissue of rats

2018 ◽  
Vol 14 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. Ebrahimi ◽  
R. Fathi ◽  
Z. Ansari Pirsaraei ◽  
E. Talebi Garakani ◽  
M. Najafi
Keyword(s):  
Weight Gain ◽  
Lipid Profile ◽  
High Intensity ◽  
High Fat Diet ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
High Fat ◽  
Insulin Effect ◽  
Significant Difference ◽  
High Intensity Interval

Lipogenic and lipolytic pathways are tightly regulated by nuclear receptors and binding proteins, such as farnesoid x receptor (FXR) and sterol regulatory element binding protein 1c (SREBP-1c). We designed this research to study the effects of high-intensity interval training (HIIT) and high-fat diet (HFD) on hepatic and adipose FXR and SREBP-1c gene expression beside the plasma levels of lipid profile and insulin. 24 male Wistar rats were randomly divided into normal (~12% fat) and HFD (~56% fat) groups with or without participating in the 8 weeks HIIT protocol. Results from two-way ANOVA and Pearson tests (P<0.05) showed that the HFD rats experienced a larger weight gain correlated with dyslipidemia and hyperinsulinemia, higher hepatic and adipose SREBP-1c expression and lower hepatic FXR expression compared with normal diet fed rats. Although HIIT rats showed higher hepatic FXR and lower hepatic and adipose SREBP-1c expression and lower weight gain compared with untrained rats, plasma lipid profile levels had not any significant difference between trained and untrained rats. Interestingly, hepatic FXR expression was negatively correlated with weight gain and SREBP-1c expression in both tissues while only the hepatic SREBP-1c was positively correlated to insulin levels. In conclusion, HFD-induced dyslipidemia could occur via the activation of the hepatic SREBP-1c pathway under the insulin effect. Although HIIT rats showed lower SREBP-1c correlated to hepatic FXR activation it seems diet is more effective on lipid profile than HIIT. Also, in presence of HFD, HIIT only affects adipose lipolysis.

scholarly journals Comparing the Effect of Two Methods of High-Intensity Interval Training (HIIT) and High-Intensity Continuous Training (HICT) on Damage and Antioxidant Indices of Liver in Male Wistar Rats

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Aghaali Ghasemnian ◽  
Mozhgan Usefpour ◽  
Ahmad Rahmani ◽  
Zeinab Iddehloei
Keyword(s):  
Superoxide Dismutase ◽  
High Intensity ◽  
Liver Tissue ◽  
Wistar Rats ◽  
Interval Training ◽  
Control Group ◽  
High Intensity Interval Training ◽  
Continuous Training ◽  
Male Wistar Rats ◽  
High Intensity Interval

Background: Nowadays, one of the widely used training methods is high-intensity interval training (HIIT). In addition, researchers have stated that long-term, high-intensity training is associated with the production of free radicals in the body. Free radicals also damage liver cells, resulting in increased liver enzymes. Objectives: The objective of this study was to investigate the effect of HIIT, compared to high-intensity continuous training (HICT), on damage and antioxidant indices of the liver in male Wistar rats. Methods: Twenty-two adult male rats were randomly divided into three groups of control (n = 6), HIIT (n = 8), and HICT (n = 8). Training protocols included HIIT and HICT on a treadmill for eight weeks (five days in a week). Standard water and food were provided for rats ad libitum. Forty-eight hours after the last training session, blood and tissue samples were collected, and serum enzymes were measured by the methods recommended by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) method. The level of enzyme activity of superoxide dismutase in the liver tissue was measured by Randox Commercial Kit (Cat. No. SD 126). One-way analysis of variance (ANOVA) and Tukey’s post hoc tests were used to analyze the data. Results: The results showed that eight weeks of HIIT and HICT had no effect on the alanine aminotransferase (ALT) level (P = 0.14), but eight weeks of HIIT significantly decreased the serum aspartate aminotransferase (AST) level compared to the control group (P = 0.04). The level of liver superoxide dismutase (SOD) enzyme activity significantly increased in the HIIT and HICT groups compared to the control group (P = 0.001). HICT also led to a significant increase in the SOD level compared to HIIT (P = 0.03). Conclusions: The research results suggested that eight weeks of HIIT led to a significant reduction in serum AST levels, and HIIT had a lower effect on the increased SOD activity in liver tissue compared to HICT. It might be stated that HIIT is safer than HICT, and it has fewer destructive effects on liver tissue.

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