Purification and preliminary X-ray analysis of YbeY from Staphylococcus aureus

Two new bis-Schiff bases containing a piperazine ring, N,N‘-bis(4-chlorobenzylidene)- and N,N‘-bis(4-cyanobenzylidene)-1,4-bis(3-aminopropyl)piperazine, were prepared by the reaction of N,N‘-bis(3-aminopropyl)piperazine with 4-chloro- and 4-cyanobenzaldehyde, respectively. The dichloro compound was fully identified by X-ray crystallography and it exhibited good antibacterial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis.

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Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

10.21203/rs.3.rs-842251/v1 ◽

2021 ◽

Author(s):

Davood Gheidari ◽

Morteza Mehrdad ◽

Saloomeh Maleki ◽

Samanesadat Hosseini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Cereus ◽

Antibacterial Properties ◽

Limited Area ◽

X Ray Diffraction ◽

X Ray ◽

Inhibition Concentration ◽

Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

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Optical characterization and concentration-dependent antibacterial activity of silver nanoparticles (AgNPs) using aqueous Melastoma malabathricum extract against Staphylococcus aureus

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3160 ◽

2020 ◽

Vol 11 (4) ◽

pp. 5382-5387

Author(s):

Irshad Ul Haq Bhat ◽

Maisarah Binti Alias

Keyword(s):

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Functional Groups ◽

Diffusion Method ◽

Synthetic Approach ◽

Potential Candidate ◽

X Ray ◽

Melastoma Malabathricum ◽

Resonance Band

The approach towards green synthetic methods has been enormously encouraged to synthesise nanoparticles for various uses. In this study, the one-pot synthetic method was adapted to synthesise silver nanoparticles (AgNPs) using Melastoma malabathricum (M. malabathricum) aqueous extract. The formation of AgNPs was confirmed by observing the results obtained by optical characterisation methods. The plasma resonance band along with shoulder at 375 nm and 595 nm, respectively, in Uv-Visible spectra supported the conversion of silver (Ag) to AgNPs reduced by functional groups present in the plant extract. The size of AgNPs was 31 nm and cubic in shape as confirmed by X-ray diffractometry (XRD) using Scherer equation. X-Ray Fluorescence (XRF) results also confirmed the presence of silver. The FTIR characterisation confirmed the presence of reducing functional groups. The antibacterial activity of AgNPs against Staphylococcus aureus (S. aureus) was carried out by disc diffusion method with increasing concentration of AgNPs, and enhanced inhibition zone was observed. The AgNPs obtained can be further explored against different bacterial strains and can a potential candidate as an antibacterial agent using the green synthetic approach.

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SINTESIS NANOKOMPOSIT Nata de coco/TiO2/Ag DAN EFEKTIVITASNYA SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus

CHEMISTRY PROGRESS ◽

10.35799/cp.14.1.2021.34130 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Heriyanto Tinentang ◽

Henry F Aritonang ◽

Harry S. J. Koleangan

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Scanning Electron Microscopy ◽

Staphylococcus Aureus ◽

Chemical Reduction ◽

Bacterial Activity ◽

Energy Dispersive ◽

X Ray ◽

Escherichia Coli Bacteria ◽

Scanning Electron

Telah dilakukan penelitian tentang kemampuan aktivitas anti bakteri untuk bakteri Staphylococcus aureus (gram positif) dan Escherichia coli (gram negatif) dengan menggunakan nanokomposit nata de coco/TiO2, nata de coco/Ag, dan nata de coco/TiO2/Ag dengan variasi konsentrasi Ag 0,5 M; 0,6 M; 0,7 M; 0,8 M dan 0,9 M menggunakan metode reduksi kimia. Nanopartikel tersebut dikarakterisasi menggunakan X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) dan anti bakteri. Untuk uji aktivitas antibakteri menggunakan metode kertas cakram dan dilakukan sebanyak dua kali ulangan untuk tiap-tiap sampel dan bakteri yang diuji. Hasil penelitian menunjukan, aktivitas anti bakteri nanokomposit yang paling baik dalam menghambat pertumbuhan bakteri adalah nanokomposit Nata de coco/TiO2/Ag mampu menghambat pertumbuhan bakteri Escherichiacoli dan Staphylococcusaureus, namun nanokomposit tersebut lebih efektif menghambat pertumbuhan bakteri Escherichiacoli.ABSRACT Research on the ability of anti-bacterial activity for Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) bacteria using nata de coco / nanocomposites TiO2, nata de coco / Ag, and nata de coco / TiO2 / Ag with variations of Ag 0,5 M; 0.6 M; 0.7 M; 0.8 M and 0.9 M using the chemical reduction method. Nanoparticles were characterized using X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spctroscopy (SEM-EDS) and anti-bacterial actvity. Test the antibacterial activity using the paper disc method and repeated two times for each sample and bacteria tested. The results showed that the good anti-bacterial activity of nanocomposites in inhibiting bacterial growth was nanocomposite nata de coco /TiO2/Ag able to inhibit the growth of Escherichia coli and S. aureus, but the nanocomposite is more effective in inhibiting the growth of Escherichia coli bacteria.

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Preliminary study on small angle X-ray scattering patterns of intact vancomycin susceptible and non-susceptible Staphylococcus aureus cells

ScienceAsia ◽

10.2306/scienceasia1513-1874.2022.008 ◽

2022 ◽

Vol 48 (1) ◽

pp. 37

Author(s):

Sujintana Wongthong ◽

Nuntaporn Kamonsutthipaijit ◽

Cholthicha Kaewhan ◽

Aroonwadee Chanawong ◽

Patcharaporn Tippayawat ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Small Angle ◽

X Ray ◽

X Ray Scattering ◽

Preliminary Study ◽

Ray Scattering

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X-Ray Crystal Structure of Staphylococcus aureus FemA

Structure ◽

10.1016/s0969-2126(02)00807-9 ◽

2002 ◽

Vol 10 (8) ◽

pp. 1107-1115 ◽

Cited By ~ 59

Author(s):

Timothy E. Benson ◽

D.Bryan Prince ◽

Veronica T. Mutchler ◽

Kimberly A. Curry ◽

Andrea M. Ho ◽

...

Keyword(s):

Crystal Structure ◽

Staphylococcus Aureus ◽

X Ray

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Rhodomyrtone, an antibotic from Rhodomyrtus tomentosa

Australian Journal of Chemistry ◽

10.1071/ch01194 ◽

2002 ◽

Vol 55 (3) ◽

pp. 229 ◽

Cited By ~ 77

Author(s):

Dachriyanus Salni ◽

M. V. Sargent ◽

B. W. Skelton ◽

I. Soediro ◽

M. Sutisna ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethyl Acetate ◽

Single Crystal ◽

Ethyl Acetate Extract ◽

Significant Activity ◽

Structural Determination ◽

X Ray ◽

And Staphylococcus Aureus ◽

Rhodomyrtus Tomentosa

The ethyl acetate extract of the leaves of Rhodomyrtus tomentosa (Ait.) Hassk. yielded rhodomyrtone (5) [6,8-dihydroxy-2,2,4,4-tetramethyl-7-(3-methyl-1-oxobutyl)-9-(2-methylpropyl)-4,9-dihydro-1H-xanthene-1,3(2H)-di-one] which had significant activity against Escherichia coli and Staphylococcus aureus. The structural elucidation followed from spectroscopic evidence and from a single-crystal X-ray structural determination.

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Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene

Natural Product Communications ◽

10.1177/1934578x1801300401 ◽

2018 ◽

Vol 13 (4) ◽

pp. 1934578X1801300

Author(s):

Daniyar Sadyrbekov ◽

Timur Saliev ◽

Yuri Gatilov ◽

Ivan Kulakov ◽

Roza Seidakhmetova ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Spatial Structure ◽

Bacterial Strains ◽

Gram Negative ◽

X Ray ◽

Cyclopropane Derivative

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.

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Exploration of the Antimicrobial and Catalytic Properties of Gold Nanoparticles Greenly Synthesized by Cryptolepis buchanani Roem. and Schult Extract

Journal of Nanomaterials ◽

10.1155/2020/1320274 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Kamonpan Wongyai ◽

Phitchayapak Wintachai ◽

Rasimate Maungchang ◽

Parawee Rattanakit

Keyword(s):

Staphylococcus Aureus ◽

Gold Nanoparticles ◽

Analytical Techniques ◽

Average Diameter ◽

Face Centered Cubic ◽

Tem Analysis ◽

X Ray ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Face Centered

A green, simple, and rapid synthesis of gold nanoparticles using plant extract, Cryptolepis buchanani Roem. and Schult, and their applications are first described in this paper. The formation of gold nanoparticles was visually observed by the appearance of a ruby red color, which was further indicated by an absorption peak at 530 nm in UV-Vis spectroscopy. Optimization of reaction parameters for the gold nanoparticles was also investigated. Various analytical techniques were employed as part of the process of characterizing the resulting gold nanoparticles. Fourier transform infrared (FTIR) analysis revealed that the phenol compounds present in the extract were responsible for gold(III) reduction and stabilization of gold nanoparticles. Transmission electron microscopy (TEM) analysis showed that the gold nanoparticles were spherical in shape with an average diameter of 11 nm. Powder X-ray diffraction (XRD) pattern indicated that the green synthesis approach produced highly crystalline, face-centered cubic gold nanoparticles. Energy-dispersive X-ray spectroscopy (EDS) measurements confirmed the presence of elemental gold in the prepared nanoparticles. The negative zeta potential value of gold nanoparticles was found to be -30.28 mV. The green synthesized gold nanoparticles expressed effective antibacterial activity against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Acinetobacter baumannii and exhibited an excellent catalytic property in terms of its reduction ability of methylene blue.

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Structural insight into the Staphylococcus aureus ATP-driven exporter of virulent peptide toxins

Science Advances ◽

10.1126/sciadv.abb8219 ◽

2020 ◽

Vol 6 (40) ◽

pp. eabb8219

Author(s):

N. Zeytuni ◽

S. W. Dickey ◽

J. Hu ◽

H. T. Chou ◽

L. J. Worrall ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Bound State ◽

Broad Spectrum Antibiotic ◽

X Ray ◽

X Ray Crystallography ◽

Open Conformation ◽

Mechanistic Insight ◽

Cytolytic Peptides ◽

Peptide Toxins ◽

Insight Into

Staphylococcus aureus is a major human pathogen that has acquired alarming broad-spectrum antibiotic resistance. One group of secreted toxins with key roles during infection is the phenol-soluble modulins (PSMs). PSMs are amphipathic, membrane-destructive cytolytic peptides that are exported to the host-cell environment by a designated adenosine 5′-triphosphate (ATP)–binding cassette (ABC) transporter, the PSM transporter (PmtABCD). Here, we demonstrate that the minimal Pmt unit necessary for PSM export is PmtCD and provide its first atomic characterization by single-particle cryo-EM and x-ray crystallography. We have captured the transporter in the ATP-bound state at near atomic resolution, revealing a type II ABC exporter fold, with an additional cytosolic domain. Comparison to a lower-resolution nucleotide-free map displaying an “open” conformation and putative hydrophobic inner chamber of a size able to accommodate the binding of two PSM peptides provides mechanistic insight and sets the foundation for therapeutic design.

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