Rhodomyrtone, an antibotic from Rhodomyrtus tomentosa

2002 ◽  
Vol 55 (3) ◽  
pp. 229 ◽  
Author(s):  
Dachriyanus Salni ◽  
M. V. Sargent ◽  
B. W. Skelton ◽  
I. Soediro ◽  
M. Sutisna ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Single Crystal ◽  
Ethyl Acetate Extract ◽  
Significant Activity ◽  
Structural Determination ◽  
X Ray ◽  
And Staphylococcus Aureus ◽  
Rhodomyrtus Tomentosa

The ethyl acetate extract of the leaves of Rhodomyrtus tomentosa (Ait.) Hassk. yielded rhodomyrtone (5) [6,8-dihydroxy-2,2,4,4-tetramethyl-7-(3-methyl-1-oxobutyl)-9-(2-methylpropyl)-4,9-dihydro-1H-xanthene-1,3(2H)-di-one] which had significant activity against Escherichia coli and Staphylococcus aureus. The structural elucidation followed from spectroscopic evidence and from a single-crystal X-ray structural determination.

scholarly journals The Effectiveness of Ethyl Acetate Extract From Breadfrui (Artocarpus Altilis) Leaves to Inhibit Diarrhea-Causing Bacteria

Biomedika ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 29-35
Author(s):  
Yusianti Silviani ◽  
Ardy Prian Nirwana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Artocarpus Altilis ◽  
Inhibition Zones ◽  
And Staphylococcus Aureus

Diarrhea is a health problem that commonly occurs in developing countries. Bacteria that cause diarrhea are among others Escherichia coli, Salmonella typhosa and Staphylococcus aureus. This research aimed to investigate the diameters of inhibition zones of breadfruit leaf (A. altilis) ethyl acetate extract in different concentrations against the growth of Escherichia coli, Salmonella typhosa and Staphylococcus aureus. The research applied an experimental laboratory by using a post-test control group design. This research was performed at the Bacteriology Laboratory of STIKES Nasional by using the diffusion disk method. The research showed the radical zone diameters against Escherichia coli with the concentrations of  20%, 40%, 60%, 80%, and 100%,  were 6.16 mm, 6.41 mm, 6.74 mm, 7.49 mm, and 7.79 mm, respectively.  The inhibition zones against Staphylococcus aureus were 8.15, mm 9.43 mm, 10.29, 10.38 mm and 11.42 mm, while against Salmonella typhosa were 7.94 mm, 8.87 mm, 10.15 mm, 10.26 mm, and 11.23 mm, respectively. The results of the ANOVA test showed the p-value=0.00 and the results of the LSD test revealed the differences in the inhibition effects of A. altilis leaf extract against the growth of E. coli, S. typhosa and S. aureus. This study concludes that concentration variations of Artocarpus altilis leaf ethyl acetate extract can inhibit the growth of  Escherichia coli, Staphylococcus aureus, and Salmonella typhosa.

scholarly journals Ultrastructural changes in methicillin-resistant Staphylococcus aureus (MRSA) induced by metabolites of thermophilous fungi Acrophialophora levis

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258607
Author(s):  
Shivankar Agrawal ◽  
Jusna Nandeibam ◽  
Indira Sarangthem
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ultrastructural Changes ◽  
Total Phenolic ◽  
Resistant Bacteria ◽  
Significant Activity ◽  
Scanning Electron Micrographs ◽  
Methicillin Resistant

Staphylococcus aureus and Methicillin-resistant S. aureus (MRSA) remains one of the major concerns of healthcare associated and community-onset infections worldwide. The number of cases of treatment failure for infections associated with resistant bacteria is on the rise, due to the decreasing efficacy of current antibiotics. Notably, Acrophialophora levis, a thermophilous fungus species, showed antibacterial activity, namely against S. aureus and clinical MRSA strains. The ethyl acetate extract of culture filtrate was found to display significant activity against S. aureus and MRSA with a minimum inhibitory concentration (MIC) of 1 μg/mL and 4 μg/mL, respectively. Scanning electron micrographs demonstrated drastic changes in the cellular architecture of metabolite treated cells of S. aureus and an MRSA clinical isolate. Cell wall disruption, membrane lysis and probable leakage of cytoplasmic are hallmarks of the antibacterial effect of fungal metabolites against MRSA. The ethyl acetate extract also showed strong antioxidant activity using two different complementary free radicals scavenging methods, DPPH and ABTS with efficiency of 55% and 47% at 1 mg/mL, respectively. The total phenolic and flavonoid content was found to be 50 mg/GAE and 20 mg/CAE, respectively. More than ten metabolites from different classes were identified: phenolic acids, phenylpropanoids, sesquiterpenes, tannins, lignans and flavonoids. In conclusion, the significant antibacterial activity renders this fungal strain as a bioresource for natural compounds an interesting alternative against resistant bacteria.

scholarly journals Antibacterial Activity of Biologically Synthesized Silver and Zinc Nanoparticles Using Allcemilla vulgaris (Layd’s Mantle) Leaf Extract

2019 ◽  
pp. 1-11
Author(s):  
Dilek Demirezen-Yilmaz ◽  
Fatih Doğan Koca ◽  
Nurhan Ertaş-Onmaz
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Escherichia Coli O157 ◽  
X Ray Diffraction ◽  
Zinc Nanoparticles ◽  
X Ray ◽  
Vis Spectroscopy ◽  
And Staphylococcus Aureus ◽  
Antibacterial Potential

In this study, the powder of Allcemilla vulgaris was used in the sythesis of silver and zinc nanoparticle. Biologically synthesized nanoparticles were characterized using Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, X-ray diffraction (XRD) and Zeta potential and then evaluated for antibacterial potential using micro dilution broth method. The minimum inhibitory concentration values of AgNP were 4.25 µg/mL and 6.64 µg/ mL for Escherichia coli O157:H7 and Staphylococcus aureus, respectively. Similarly, the MIC values of ZnNP were 3.32 µg/mL and 6.25 µg/mL, respectively for Escherichia coli O157:H7 and Staphylococcus aureus.

scholarly journals Synthesis, Structures, and Antibacterial Activities of Hydrazone Compounds Derived from 4-Dimethylaminobenzohydrazide

2021 ◽  
Vol 68 (3) ◽  
pp. 567-574
Author(s):  
Guo-Xu He ◽  
Ling-Wei Xue
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Nmr Spectra ◽  
Antibacterial Activities ◽  
13C Nmr Spectra ◽  
X Ray Diffraction ◽  
X Ray ◽  
Mtt Method ◽  
Antibacterial Material ◽  
And Staphylococcus Aureus

A series of three new hydrazone compounds derived from the condensation reactions of 4-dimethylaminobenzohydrazide with 4-dimethylaminobenzaldehyde, 2-chloro-5-nitrobenzaldehyde and 3-methoxybenzaldehyde, respectively, were prepared. The compounds were characterized by elemental analysis, infrared and UV-vis spectra, HRMS, 1H NMR and 13C NMR spectra, and single crystal X-ray diffraction. Crystals of the compounds are stabilized by hydrogen bonds. The compounds were assayed for antibacterial (Bacillus subtilis, Escherichia coli, Pseudomonas fluorescence and Staphylococcus aureus) and antifungal (Aspergillus niger and Candida albicans) activities by MTT method. The results indicated that compound 2 is an effective antibacterial material.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM ETHYL ACETATE EXTRACT OF HYDROCOTYLE SIBTHORPIODES AGAINST MULTI DRUG RESISTANT MICROBES.

2017 ◽  
Vol 10 (6) ◽  
pp. 263 ◽  
Author(s):  
Manashi Garg ◽  
Banasmita Devi ◽  
Rashna Devi
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Metallic Silver ◽  
Klebsiella Pneumonia ◽  
Synthesis Methods ◽  
Drug Resistant ◽  
Bacterial Isolates ◽  
And Staphylococcus Aureus

 Objectives: Hydrocotyle sibthorpiodes is known to contain several phytoconstituents which are constantly involved in the formation of Silver nanoparticles that may affect several multi-drug resistant microbes. Therefore, the study was undertaken to evaluate the efficacy of different concentration of nano silver solution on three bacterial isolates. It was also aimed to qualitatively assess the different phytoconstituents responsible for the synthesis. Methods: Three bacterial isolates of Klebsiella pneumonia, Pseudomonas aeroginosa and Staphylococcus aureus were identified. Synthesis of AgNPs with different concentration (2/4/6/8/10μl/ml) was done and applied to the selected isolates. The phytochemical compounds of the ethyl acetate extract were assayed by several colored reactions qualitatively. Results: The size and stability biosynthesis of the metallic silver nanoparticles were confirmed by photophysical characterization as well as SEM (Scanning Electron Microscopy), XRD (XRay Diffraction), Zeta potential and DLS (Dynamic Light Scattering) with an average size of 13.37 ±10 nm. The increasing concentration of the particle solution showed significant inhibition zone for all the three isolates viz., Klebsiella pneumonia, Pseudomonas aeroginosa and Staphylococcus. aureus showing the value of 3.0±0.17, 2.7±0.32 and 3.6±0.57 respectively for 10 μl/ml concentration. Phytochemical screening of the whole plant extract also revealed an array of bioactive compounds which may have an effective role in the reduction process. Conclusion: The study demonstrated a simple, efficient and eco-friendly synthesis of stable silver nanoparticles from the ethyl acetate extract of Hydrocotyle sibthorpiodes having fairly superior antimicrobial activity against human pathogens.

scholarly journals Antimicrobial Activity of Arugula (Eruca Sativa) Leaves on Some Pathogenic Bacteria

2019 ◽  
Vol 11 (3) ◽  
pp. 10 ◽  
Author(s):  
Samar Qaddoumi ◽  
Nasser El-Banna
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Erwinia Amylovora ◽  
Ethyl Acetate Extract ◽  
Water Extract ◽  
Eruca Sativa ◽  
And Staphylococcus Aureus

Arugula (Eruca sativa) is a green leafy vegetable; whose flowers, seed pods and seeds are edible. It’s packed with vital nutrients that can help you step up your health without spending too much money. This study aims to fight pathogenic bacteria whether they affect plants or humans by stopping their growth and work as antibiotics. In the present study, water extract of Arugula leaves was effective against Escherichia coli HAS 11 (19mm) and Staphylococcus aureus HAS 1 (12mm), but no activity was observed against Erwinia amylovora HAS 12 and Bacillus cereus HAS 2. In the case of ethyl acetate extract, no antimicrobial activity against tested microorganisms, Staphylococcus aureus HAS 1, Bacillus cereus HAS 2, Escherichia coli HAS 11 and Erwinia amylovora HAS 12 was seen.

scholarly journals Analysis of The Phytochemical Characteristics of Jasmine Flower Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 15 (3) ◽  
pp. 4
Author(s):  
Nathasa Sihite
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Agricultural Product ◽  
Disc Diffusion Method ◽  
Water Solvent ◽  
Jasminum Sambac ◽  
And Staphylococcus Aureus

Indonesia is a famous country had the diversity of plants, especially agricultural product and herbs, they had a potential of antimicrobial as a like Jasmine Flower (Jasminum sambac Ait). This study Indonesia is a famous country had the diversity of plants, especially agricultural product and herbs, they had a potential of antimicrobial as a like Jasmine Flower (Jasminum sambac Ait). This study aimed to determine the phytochemical and antimicrobial activity of jasmine flower. Extraction was done by maceration, such as using water solvent, methanol, ethyl acetate, and hexane as the solvent. The sample bacteria used were Escherichia coli and Staphylococcus aureus. This study used disc diffusion method, with a complete 2-factor random design and 4 replications. Processing of the data is using ANOVA. The results showed that the jasmine extracts with a concentration of 25%, 50%, 75% and 100% had an effect to inhibit of the growth of the bacteria. Jasmine extract with ethyl acetate solvent was the most effective to inhibit the growth of bacteria. It was known that jasmine flower extract is one of the good natural antimicrobials gave an inhibitory effect to the growth of Escherichia coli and Staphylococcus aureus bacteria. Keywords: jasmine, Escherichia coli, Sthaphylococcus aureus, antimicrobials

scholarly journals Antimicrobial activity from endophytic fungi Arthrinium state of Apiospora montagnei Sacc. and Papulaspora immersa

2010 ◽  
Vol 53 (3) ◽  
pp. 629-632 ◽  
Author(s):  
Henrique Pereira Ramos ◽  
Glaucia Hollander Braun ◽  
Mônica Tallarico Pupo ◽  
Suraia Said
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Smallanthus Sonchifolius ◽  
Crude Extracts ◽  
First Time ◽  
Kocuria Rhizophila

Papulaspora immersa and Arthrinium state of Apiospora montagnei Sacc. were isolated from the roots of Smallanthus sonchifolius (yacón). The crude extracts from their cultures inhibited the growth of Staphylococcus aureus, Kocuria rhizophila, Pseudomonas aeruginosa and Escherichia coli. The more relevant results were observed in the ethyl acetate extract from P immersa against P aeruginosa (90 µg/mL) and ethyl acetate extract from Arthrinium state of A montagnei Sacc. against P aeruginosa (160 µg/mL). The two endophytic fungi isolated from yacón roots as well as their antimicrobial activity detected in the crude extracts cultures were being reported for the first time.

scholarly journals Exploration of Bioactive Compounds Potency of Extract Namanereis sp. (Polychaeta: Annelida) as an Antibacterial Agent Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 26 (3) ◽  
pp. 182-188
Author(s):  
Delianis Pringgenies ◽  
Endah Sari ◽  
Widianingsih Widianingsih ◽  
Anuwat Nateewathana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Antibacterial Agent ◽  
Pathogenic Bacteria ◽  
Laboratory Method ◽  
Gas Chromatography Mass Spectrometry ◽  
Extract Content ◽  
And Staphylococcus Aureus

Namanereis sp. belongs to class Polychaeta, a group of main benthic community in marine ecosystems. The purpose of this study to determine the potential of bioactive compounds from Namanereis sp. extract as an antibacterial agent and to determine its extract content through Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Samples of Namaneries sp. were collected from Demak Waters, Central of Java. This research used experimental laboratory method. Samples were extracted with non-polar (N-Hexane), semi-polar (Ethyl acetate) and polar (Methanol) solutions. Different concentrations of extraction, i.e. 5, 10, 20, 40, 80, and 100 µg.disk-1. then were tested against pathogenic bacteria Escherichia coli and Staphylococcus aureus. The extract content analysis was obtained using the GC-MS method.  The results showed that Namanereis sp. extract had a potential as an antibacterial agent. The highest inhibition zone was seen in the fraction with ethyl acetate solvent at a concentration of 100%, namely 13±3.05 mm in E. coli and 14±3.04 mm in S. aureus, which then was analysed with GC-MS. Its results found 20 bioactive compounds in the extract. The five most dominant compounds determined by the highest peak were Dihydrojasmone (12.31%), Hexanoic acid (11.49%), Cholesterol (7.66%), Cyclohexanol (7.53%) and Isopropyl Palmitate (7.52%). The research concluded that Namanereis sp. possessed antibacterial potency and contained several bioactive compounds.

scholarly journals ANTIBACTERIAL ACTIVITIES OF BELIMBING WULUH (AVERRHOA BILIMBI L.) ETHYL ACETATE EXTRACT ON GEL FORMULATED AGAINST PROPIONIBACTERIUM ACNES AND STAPHYLOCOCCUS AUREUS

2020 ◽  
pp. 224-228
Author(s):  
DEWI PERTIWI ◽  
IHSANUL HAFIZ ◽  
WARDATUL JANNAH ◽  
HANAFIS SASTRA WINATA ◽  
MELIA SARI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Extract ◽  
Skin Irritation ◽  
Diffusion Method ◽  
Skin Bacteria ◽  
Dispersion Test ◽  
And Staphylococcus Aureus

Objective: Averrhoa bilimbi is a plant that is used as a food spice and traditional medicine in Indonesia. The purpose of this study was to obtain a gel formula and measure its activity in inhibiting the growth of skin bacteria, namely Propionibacterium acnes and Staphylococcus aureus. Methods: The gel formulation was tested organoleptically, homogeneity, pH, dispersibility test, and skin irritation test. Antibacterial activity test using agar diffusion method, inhibition of bacterial growth was compared with clindamycin as a positive control. Results: All gel formulations showed good homogeneity, pH of the preparations ranged from 4.5 to 6.1, the dispersion test was 5.5-6.5 cm, and none of them caused irritation to the skin. The results of the inhibitory activity against the growth of Propionibacterium acnes bacteria on concentrations 10, 15, and 20% were 16.67 + 0.40, 22.70 + 0.32 and 28.10 + 0.36 and Staphylococcus aureus bacteria were 18.53 + 0.22, 24.16 + 0.29 and 30.40 + 0.4.  The inhibitory activity of clindamycin against Propionibacterium acnes and Staphylococcus aureus bacteria were 35.33+0.29 and 36.30+0.37. Conclusion: Ethyl acetate extract gel Averrhoa bilimbi had good activity in inhibiting the growth of Propionibacterium acnes and Staphylococcus aureus bacteria and has the potential to be used as an herbal anti-acne drug.

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