scholarly journals Ochratoxin contamination in pig feed from pig farming centres in Indonesia

2021 ◽  
pp. 11-17
Author(s):  
Variena Intansari ◽  
Agustin Indrawati ◽  
Sri Murtini
Keyword(s):  
Animal Feed ◽  
Culture Media ◽  
Enzyme Linked Immunosorbent Assay ◽  
National Agency ◽  
Pig Feed ◽  
The Republic ◽  
Pellet Feed ◽  
North Sumatra ◽  
Penicillium Spp ◽  
Meat Bone Meal

Ochratoxin A (OTA) is the most common toxin found in nature. Ochratoxin is a metabolic product by Aspergillus spp. and Penicillium spp. OTA produced by many Aspergillus or Penicicillium species that contaminate animal feeds can cause diseases. This study aims to determine the incidence of ochratoxin contamination in pig feed such as pellets, meat bone meal (MBM), and tofu by products. The sampling method used the disease detection formula to collect 36 samples 36 taken from several regions. North Sumatra, East Nusa Tenggara, Bali, Bulan Island, Solo, Lampung, Banten, Bogor and Jakarta. Ochratoxin contamination in animal feed was detected based on the Enzyme Linked Immunosorbent Assay (ELISA) using Agraquant® ochratoxin assay following the manufacturer’s protocol. The analysis showed that 6 of the 36 samples (16.7%) were tested positive for ochratoxin contamination. Pellets and MBM were the feed and feedstuff which were found to contain ochratoxin. The concentration of ochratoxin in MBM was below the limit set by the National Agency of Drug and Food Control of the Republic of Indonesia (5 ppb). The high contamination of ochratoxin was found in pellets from Lampung (19 ppb). The fungi found to dominates the culture media and come from pellet feed were Aspergillus flavus and Aspergillus niger.

scholarly journals Screening of mycotoxins in animal feed from the region of Vojvodina

2009 ◽  
pp. 87-96 ◽  
Author(s):  
Bojana Kokic ◽  
Ivana Cabarkapa ◽  
Jovanka Levic ◽  
Anamarija Mandic ◽  
Jovana Matic ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Animal Feed ◽  
Screening Method ◽  
Rapeseed Meal ◽  
Enzyme Linked Immunosorbent Assay ◽  
Alfalfa Meal ◽  
Soybean Cake ◽  
And Storage ◽  
Representative Samples ◽  
Meat Bone Meal

This paper shows the results of screening of mycotoxins in animal feed originating from the region of Vojvodina. Permanent screening is needed on all levels of production and storage, as well as the use of known methods to reduce mould contamination or toxin content in feedstuffs and feed. A total of 56 representative samples were collected from feed companies from the region of Vojvodina. Samples were collected during February 2009. The collected samples included 41 samples of feedstuffs (soybean, soybean meal, soybean grits, soybean cake, maize, sunflower meal, barley, wheat feed flour, rapeseed meal, dehydrated sugar beet pulps, alfalfa meal, yeast, dried whey, fish meal, meat-bone meal) and 15 samples of complete feedingstuffs. The amounts of aflatoxins, ochratoxin A, zearalenone, fumonisin and deoxynivalenol were determined. Screening method for the analysis was done using Neogen Veratox? testing kits. The test itself is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA). Mycotoxins were present in 71.4% of the samples, but the values determined were below the maximum allowed limits for both Serbian and EC reference values. Zearalenone was found with the highest incidence (57.1% of samples), followed by ochratoxin A (37.5%), fumonisin (33.9%), deoxynivalenol (14.3%) and aflatoxins (3.6%).

Peranan Ombudsman Republik Indonesia Perwakilan Sumatera Utara Dalam Pengawasan Pelayanan Dinas Kependudukan dan Catatan Sipil Kota Medan

2019 ◽  
Vol 1 (1) ◽  
pp. 1-12
Author(s):  
Putri Ananda Sari ◽  
Abdul Kadir ◽  
Beby Mashito Batu Bara
Keyword(s):  
Service Provider ◽  
Qualitative Approach ◽  
First Report ◽  
The North ◽  
Supervision Process ◽  
The Republic ◽  
North Sumatra

This study aims to determine the role of the Ombudsman of the Republic of Indonesia in North Sumatra Representative in the Supervision of Population and Civil Registry Service in Medan City. This study uses a qualitative approach with descriptive methods describing information about the data obtained from the field in the form of written and oral data from the parties studied. Data is collected based on interviews and documentation. The results of this study indicate that the role of the Ombudsman of the Republic of Indonesia in North Sumatra was carried out in the form of external supervision. External supervision is supervision carried out by the Ombudsman of the Republic of Indonesia Representative of North Sumatra to the Medan Population and Civil Registry Service. Actions taken in the supervision process are incoming reports, follow-up of the first report and follow-up of the report. Based on the research that has been carried out, it has been concluded that the role of the Ombudsman of the Republic of Indonesia in the North Sumatra Representative in supervising the service provider of the Population and Civil Registry services is carried out in the form of external supervision. In supervising the handling of public reports of alleged poor service in the area of population administration, it has been effective, with several efforts to handle reports such as: (1) Clarification; (2) Investigation; (3) Recommendations; (4) Monitoring.

scholarly journals Recombinant γY278H Fibrinogen Showed Normal Secretion from CHO Cells, but a Corresponding Heterozygous Patient Showed Hypofibrinogenemia

2021 ◽  
Vol 22 (10) ◽  
pp. 5218
Author(s):  
Tomu Kamijo ◽  
Takahiro Kaido ◽  
Masahiro Yoda ◽  
Shinpei Arai ◽  
Kazuyoshi Yamauchi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cho Cells ◽  
Fibrinogen Level ◽  
Culture Media ◽  
Chinese Hamster ◽  
Plasma Fibrinogen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cho Cell ◽  
Accelerated Degradation ◽  
Heterozygous Patient

We identified a novel heterozygous hypofibrinogenemia, γY278H (Hiroshima). To demonstrate the cause of reduced plasma fibrinogen levels (functional level: 1.12 g/L and antigenic level: 1.16 g/L), we established γY278H fibrinogen-producing Chinese hamster ovary (CHO) cells. An enzyme-linked immunosorbent assay demonstrated that synthesis of γY278H fibrinogen inside CHO cells and secretion into the culture media were not reduced. Then, we established an additional five variant fibrinogen-producing CHO cell lines (γL276P, γT277P, γT277R, γA279D, and γY280C) and conducted further investigations. We have already established 33 γ-module variant fibrinogen-producing CHO cell lines, including 6 cell lines in this study, but only the γY278H and γT277R cell lines showed disagreement, namely, recombinant fibrinogen production was not reduced but the patients’ plasma fibrinogen level was reduced. Finally, we performed fibrinogen degradation assays and demonstrated that the γY278H and γT277R fibrinogens were easily cleaved by plasmin whereas their polymerization in the presence of Ca2+ and “D:D” interaction was normal. In conclusion, our investigation suggested that patient γY278H showed hypofibrinogenemia because γY278H fibrinogen was secreted normally from the patient’s hepatocytes but then underwent accelerated degradation by plasmin in the circulation.

MYCOTOXINS IN BEANS OF PEANUTS GROWN IN TAJIKISTAN

2018 ◽  
Vol 1 (4) ◽  
pp. 74-77
Author(s):  
Sh. I. Razokov ◽  
◽  
D. M. Mirzoev ◽  
G. P. Kononenko ◽  
A. A. Burkin ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Cyclopiazonic Acid ◽  
Practical Significance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Systems ◽  
The Republic ◽  
Combined Contamination

The article presents the results of an extensive mycotoxicological examination of 11 samples of peanut beans grown in two regions of the Republic of Tajikistan. The determination of 16 mycotoxins was carried out by indirect competitive enzyme-linked immunosorbent assay using commercial and certified research test systems. It has been established that for peanut beans in this area, a combined contamination by a group of sanitary-significant mycotoxins, including diacetoxyscirpenol, alternariol, ochratoxin A, PR-toxin and cyclopiazonic acid, is characteristic. The prospects of further research and the practical significance of the results are discussed.

scholarly journals New Model of Anti Radicalisme Learning for Moslem Teacher

2016 ◽  
Vol 1 ◽  
pp. 36-43
Author(s):  
Ucok Agus Saputra
Keyword(s):  
Character Education ◽  
Religious Education ◽  
Educational Institutions ◽  
New Order ◽  
Religious Groups ◽  
National Agency ◽  
New Model ◽  
Islamic Movements ◽  
The People ◽  
The Republic

Post-fall of the New Order regime, the action of Islamic radicalism spreads out fast in Indonesia. Unluckily, actors and the perpetrators are mostly Muslimand claiming graduated from Islamic educational institutions. Radical Islamic movements nowadays are worrying the people and threaten the life of the nation that contains the values of pluralism, tolerance, and acculturative, including in religion. According to way of life on Indonesian, Pancasila, and the 1945 Constitution and the Republic of Indonesia which is the basics of nation and state are also threatened. even their movement tends to do with violence. Proven by the presence of several violent incidents that resulted in deaths were not counted. (National Agency for Counter of Terrorism, 28/8/2002). Therefore, Indonesia  (and even the world) needs strategies to prevent the anti-radicalism and terrorism. Learning the last fact, education that be designed by conforming new model of learning for Moslem Teacher is absolutely needed. The approach to the handle the Islamic radicalism must always be strived, One of models is through character education. Education is partly done by reconstructing the Islamic Religious Education (PAI) that is taught in educational institutions. Reconstruction of PAI should be able to produce learners that possess attitude of tolerant, moderate and inclusive. In short, there is a tendency of systematic efforts made by certain religious groups to teach the doctrine of religious hardliners among students of Elementary School (SD), that often reborn radicalization. Here, the writer will show how Moslem teacher can take anti-radicalims learing by some new models that can be implemented, namely the de-radicalization preventive, de-radicalization preservative against moderate Islam, and  curative deradicalised.

Ginsenoside Rb2 Ameliorates LPS-Induced Inflammation and ER Stress in HUVECs and THP-1 Cells via the AMPK-Mediated Pathway

2020 ◽  
Vol 48 (04) ◽  
pp. 967-985
Author(s):  
Jaw Long Sun ◽  
A.M. Abd El-Aty ◽  
Ji Hoon Jeong ◽  
Tae Woo Jung
Keyword(s):  
Cell Adhesion ◽  
Er Stress ◽  
Culture Media ◽  
Enzyme Linked Immunosorbent Assay ◽  
Activity Measurement ◽  
Cell Viability Assay ◽  
Necrosis Factor Alpha ◽  
Monocyte Chemoattractant Protein 1 ◽  
Ampk Phosphorylation ◽  
Ginsenoside Rb2

Inflammation and endoplasmic reticulum (ER) stress have been documented to contribute to the development of atherosclerosis. Ginsenoside Rb2 has been reported to exhibit antidiabetic effects. However, the effects of Rb2 on atherosclerotic responses such as inflammation and ER stress in endothelial cells and monocytes remain unclear. In this study, the expression of inflammation and ER stress markers was determined using a Western blotting method. Concentrations of tumor necrosis factor alpha (TNF[Formula: see text]) and monocyte chemoattractant protein-1 (MCP-1) in culture media were assessed by enzyme-linked immunosorbent assay (ELISA) and apoptosis was evaluated by a cell viability assay and a caspase-3 activity measurement kit. We found that exposure of HUVECs and THP-1 monocytes to Rb2 attenuated inflammation and ER stress, resulting in amelioration of apoptosis and THP-1 cell adhesion to HUVECs under lipopolysaccharide (LPS) condition. Increased AMPK phosphorylation and heme oxygenase (HO)-1 expression, including GPR120 expression were observed in Rb2-treated HUVECs and THP-1 monocytes. Downregulation of both, AMPK phosphorylation and HO-1expression rescued these observed changes. Furthermore, GPR120 siRNA mitigated Rb2-induced AMPK phosphorylation. These results suggest that Rb2 inhibits LPS-mediated apoptosis and THP-1 cell adhesion to HUVECs by GPR120/AMPK/HO-1-associated attenuating inflammation and ER stress. Therefore, Rb2 can be used as a potential therapeutic molecule for treatment of atherosclerosis.

Assessment for animal feed of maize kernels naturally-infected predominantly with Fusarium moniliforme and Diplodia maydis. I. Fungal isolations and changes in chemical composition

1992 ◽  
Vol 43 (4) ◽  
pp. 773 ◽  
Author(s):  
KC Williams ◽  
BJ Blaney ◽  
RL Dodman ◽  
CL Palmer
Keyword(s):  
Chemical Composition ◽  
Animal Feed ◽  
Ear Rot ◽  
White Variety ◽  
N Content ◽  
Acremonium Strictum ◽  
Gross Energy ◽  
Fungal Associations ◽  
Highly Correlated ◽  
Penicillium Spp

A study was made of the physical characteristics, fungal associations and the chemical composition of five batches of maize grain (designated A, B, J, M and P) damaged by ear-rot. All batches were the yellow hybrid, Dekalb XL81, except batch A which was a white variety, Dekalb DS456W. Batch A was separated by vibrating gravity grading into heavy (A-H) and light (A-L) fractions. For each batch, kernels were sorted visually into sound and mould-damaged samples to examine for any differences. The proportion of sound kernels in batches A, A-H, A-L, B, J, M and P was 874, 926, 260, 830, 854, 789 and 964 g kg-' respectively. Bulk density (r = 0.98) and 100 seed weight (r = 0.81) were highly correlated (P < 0.01) with the proportion of mould-damaged kernel in the sample. In visually sound kernels, Fusarium monilifome, Acremonium strictum and Penicillium spp. were the most prevalent fungi isolated. In visually damaged kernels, F. moniliforme was the fungus most frequently isolated other than for batch J where D. maydis was equally prevalent. Penicillium spp. and F. subglutinans were found in all batches but at low isolation frequencies. Compared to the respective sample of sound kernel, the content of crude protein and ash was increased and crude fat was decreased by the mould damage (P < 0-05); fibre, though more variable, was generally increased but gross energy was unaffected (P > 0.05). In visually sound kernels, the amounts of threonine, valine and glycine in the protein (g 16gN-1 ) decreased (P < 0.05) with increasing grain N content. In contrast, in visually mould-damaged kernels, lysine increased and histidine and cystine decreased with increasing grain N content (P < 0.05).

Assay of Gcdfp-15 by Elisa: An Available Method for in Vitro Studies of Functional Differentiation in Human Breast Cancer

1988 ◽  
Vol 74 (6) ◽  
pp. 669-674
Author(s):  
Françoise Revillion-Carette ◽  
Louis Hornez ◽  
Brigitte Vandewalle ◽  
Jean Lefebvre
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Culture Media ◽  
Functional Differentiation ◽  
In Vitro Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Breast ◽  
Breast Cyst Fluid ◽  
Breast Cyst

An enzyme-linked immunosorbent assay (ELISA) was applied to a light protein, isolated from human breast cyst fluid (BCF) termed « gross cystic disease fluid protein - 15 Kda » (GCDFP-15), a potential differentiation marker in in vitro human breast cancer studies. The detection limits of this procedure, performed in microtiter plates, were 0.5 to 250 ng/well corresponding to 10 ng/ml to 5 ng/ml of sample or antigen solution. Possible cross-reaction with various antigens, especially those found in culture media, were investigated. The correlation coefficient between enzymoassay and radioimmunoassay was 0.978. The results showed that quantification of GCDFP-15 by ELISA is a specific and highly sensitive method. This procedure may be of interest in in vitro studies on the functional differentiation of breast cancer cells.

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