YIELD AND SOWING QUALITIES OF SPRING WHEAT DEPENDING ON VARIETY AND FOLIAR TREATMENT WITH MANGANESE, COPPER AND MOLYBDENUM

Author(s):  
Dmitry Ilyich Ivanov ◽  
Natalia Nikolaevna Ivanova ◽  
Lyudmila Nikolaevna Prokina
Author(s):  
V.M. Pakhomova ◽  
◽  
A.I. Daminova ◽  
A.Yu. Kozhevnikov ◽  
I.V. Galiyakhmetov ◽  
...  

The purpose of the article was to study the resistance of spring wheat under the influence of foliar treatment of plants with silicate bacteria according to lipid peroxidation (LP) and yield. The studies were conducted on spring wheat (variety ‘MiS’). The cultivation technology is typical wheat in this agroclimatic zone. The foliar treatment was carried out with a culture of bacteria Bacillus oligonitrophilus by spraying at the rate of 0.5 l/m2 of bacteria suspension containing 109 cells/ml (equally to 100% concentration). Field experiment design: option 1 – plants without treatment (control); option 2 – treatment with B. oligonitrophilus fertilizers at the stage of tillering; option 3 – treatment with B. oligonitrophilus fertilizers at the stage of tillering/stem elongation; option 4 – treatment with B. oligonitrophilus at the stage of tillering/stem elongation/heading. The activity of LP in wheat leaves was identified according to the content of malondialdehyde (MDA). The content of MDA was determined photometrically during interaction with thiobarbiturate. Wheat yield increase was observed in options 3 and 4 (by 11 and 15%, respectively). The content of MDA decreased in all phases of wheat vegetation in the same experimental options (by 14 and 21%, respectively), which indicated the antioxidant effect of the studied preparation. Thus, the bacterization of wheat with B. oligonitrophilus leads to the plant resistance increase. The protective effect is probably due to the action of a complex of their biologically active substances (metabolites) including the antioxidant mechanism of action.


2020 ◽  
Vol 196 (5) ◽  
pp. 20-26
Author(s):  
Galina Rabinovich ◽  
Yuliya Smirnova ◽  
Natal'ya Fomicheva

Abstract. The purpose of the research was to determine the most effective technological method of applying the LPB biological preparation on spring wheat crops. Research methodology and methods. Studies were conducted on sod-podzolic soil in 2017–2019 in the Tver region. The composition of the LPB includes agronomically useful microflora (from n×109 to n×1012 CFU/ml), physiologically active substances, a complex of macro- and micronutrients. The following technological techniques were studied: seed treatment before sowing, double foliar treatment of vegetating plants, and combining these techniques. Results. All the studied technological methods of application of LPB contributed to an increase in the yield of spring wheat grain. The highest yield on average for three years of research was obtained by combining the methods of seed treatment with a 1% solution of LPB and foliar treatment plants at a dose of 3 l/ha – 2.96 t/ha (in the control of 2.63 t/ha). The maximum yield is formed primarily by increasing the productivity of stems (95 %). In addition, the use of LPB in all research variants contributed to a significant increase in the number of grains in the ear by 1.2–1.9 PCs, there was a tendency to increase the mass of 1000 grains. Observed an increase in the nitrogen content in the soil in the variants with the introduction of LPB due to the activation of the nitrogen-transforming microflora, which is confirmed by correlations. The increase in nitrogen nutrition of wheat and the biochemical processes that take place in plants under the influence of LFB, led to an increase in the protein content of wheat grain. Scientific novelty. A new promising biological product of the LPB is proposed, for which the most effective agrotechnological method of application for spring wheat crops is selected, which allows to increase the yield of this crop, its quality, as well as improve the agrochemical and microbiological parameters of the soil.


2017 ◽  
Vol 4 (2) ◽  
pp. 28-36
Author(s):  
V. Patyka ◽  
H. Huliaieva ◽  
I. Tokovenko ◽  
V. Maksin ◽  
V. Kaplunenko

Aim. Complex study on the physiological effect of citrate chelate nanoparticles of silver and copper on spring wheat plants under presowing soaking of seeds and foliar treatment of plants. Methods. Physiological, biochemical, bio- physical, microbiological, statistical. Results. The data on the physiological effect of the presowing soaking of seeds in the solutions of citrate chelate nanoparticles of silver and copper on growth processes and photochemical activity of leaves in laboratory conditions have been studied, analyzed, and summarized along with the effect of foliar treatment of spring wheat plants using the solutions of nanoparticles, and simulated phytoplasma (Acholeplasma laidlawii var. granulum st. 118) on the elements of the performance of spring wheat plants in fi eld conditions. It was demonstrated that there was a considerable stimulating effect of presowing soaking of wheat seeds in 0.5 % and 1 % solutions of nanocomposites on the growth of leaves and mass accumulation of 6–7-day old sprouts. A considerable increase in the photochemical activity of the leaves of 7-day-old sprouts was found after presowing soaking in 0.5 % and 1 % solu- tions of nanocomposites which is in good agreement with the activation of growth processes. The negative effect of phytopathogens on the elements of grain productivity, fullness of grain in particular, was reduced in fi led conditions when wheat plants were infected and then treated with nanocomposites, though there was a weak phytotoxic effect of foliar treatment of intact plants with 1 % solution. Conclusions. A considerable stimulating physiological effect on the growth of leaves and mass accumulation of 6–7-day-old sprouts was established after the presowing soaking of spring wheat plants in 1 % and 0.5 % solutions of citrate chelate nanoparticles of silver and copper. The increase in photochemical activity was noted in the leaves of 7-day-old wheat plants in these conditions, namely, the increase in photochemistry effi ciency PSII, considerable decrease in stationary fl uorescence and considerable – almost trip- le – increase in the value of induction coeffi cient Ki (correlating with the activity of ribulose biphosphate carboxylase (RuBPco)) which testifi es to the increase in the intensity of photosynthetic processes in the leaf apparatus of juvenile wheat plants. The fi eld experiment established that infecting wheat plants with phytoplasma (A. laidlawii) resulted in the reduction of the grain productivity indices: the weight of 1,000 grains and grain productivity in g/plant – by 14.6 and 35.5 % respectively, whereas treating with 1 % solution of silver and copper nanocomposites inhibited this process to some extent, promoting the increase in these parameters almost to the control level. The treatment of infected plants with nanoparticles led to a notable increase in the percentage of full grains. There was a weak phytotoxic effect after foliar treatment of intact wheat plants using nanocomposite solution.


2017 ◽  
Vol 12 (2) ◽  
pp. 34-37
Author(s):  
Валентина Пахомова ◽  
Valentina Pahomova ◽  
Насия Даминова ◽  
Nasiya Daminova ◽  
Наталья Кузнецова ◽  
...  

The spectrum of soluble proteins of spring wheat leaves was studied for foliar treatment with chelated copper, molybdenum-containing microfertilizer ZhUSS-2 (0.1% solution). Electrophoretically, polypeptides with a molecular mass of 13.2 - 145 kD were detected. The qualitative composition of soluble proteins in the leaves of spring wheat plants under the influence of foliar treatment during the vegetation did not change, however, their quantitative changes were observed. During a single treatment of spring wheat in the tillering phase, an increase in polypeptides with a molecular mass of 145 and 94 kD was recorded; the content of polypeptides with molecular weights of 13.2 - 66 kD decreased in comparison with the control sample. When the plants were sprayed twice in the tillering and tubing phases, the content of these polypeptides as well as polypeptides with molecular weights of 45 and 41 kD increased. The content of other detected polypeptides also decreased compared to the control variant. During a three-fold treatment of spring wheat plants in the tillering, tubing and blossoming-flowering phases with the ZhUSS-2 preparation, the number of all detected polypeptides increased in comparison with the control sample. Thus, the response of the metabolism of soluble proteins in the cells of spring wheat leaves to the action of foliar top dressing with copper, molybdenum-containing chelate microfertilizer of ZhUSS-2 brand in different phases of vegetation is different and depends on the dose of exposure. Particular attention should be paid to the role of two polypeptides in the treatment of vegetative plants by ZhUSS-2 M.M. 94 kD and, mainly, 145 kD. There is a reason to believe that copper molybdenum-containing chelate microfertilizer ZhUSS-2 exhibits the action of an abiogenic elicitor, which leads to activation of the gene apparatus of cells. The possible functional role of these proteins is discussed.


2014 ◽  
Vol 1 (3) ◽  
pp. 57-61
Author(s):  
E. Kopylov

Aim. To study the specifi cities of complex inoculation of spring wheat roots with the bacteria of Azospirillum genus and Chaetomium cochliodes Palliser 3250, and the isolation of bacteria of Azospirillum genus, capable of fi xing atmospheric nitrogen, from the rhizospheric soil, washed-off roots and histoshere. Materials and meth- ods. The phenotypic features of the selected bacteria were identifi ed according to Bergi key. The molecular the polymerase chain reaction and genetic analysis was used for the identifi cation the bacteria. Results. It has been demonstrated that during the introduction into the root system of spring wheat the strain of A. brasilensе 102 actively colonizes rhizospheric soil, root surface and is capable of penetrating into the inner plant tissues. Conclusions. The soil ascomucete of C. cochliodes 3250 promotes better settling down of Azospirillum cells in spring wheat root zone, especially in plant histosphere which induces the increase in the content of chlorophyll a and b in the leaves and yield of the crop.


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