scholarly journals Commonly Occurring Bacteria in Diabetic Foot Infections and their Sensitivity to various Antibiotics

2019 ◽  
Vol 8 (1) ◽  
pp. 8-12
Author(s):  
Muneeb -ullah ◽  
Awais Saeed Abbasi ◽  
Seemab Niaz ◽  
Wajiha Mahjbeen
Keyword(s):  
Diabetic Foot ◽  
Sampling Technique ◽  
Cross Sectional Study ◽  
Medical Sciences ◽  
Cross Sectional ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Diabetic Foot Infections ◽  
Microbiological Methods

Background: Diabetic foot infections are a common cause of morbidity in type 1 & 2 Diabetes mellitus. The selection of appropriate empirical treatment is thus essential while treating such patients. The main objective of this study was to find out most prevalent bacteria and their sensitivity to various antibiotics in patients with diabetic foot infections.Material and Methods: This cross-sectional study was conducted in the Department of Surgery, Pakistan Institute of Medical Sciences (PIMS), Islamabad from January 2017 to December 2017. A total 118 patients were included through consecutive sampling technique. Samples were collected in the form of swab, pus or tissue material and were cultured on blood agar and microorganisms were identified using standard microbiological methods. Antimicrobial sensitivity was also checked. StatisticalPackage for Social Sciences (SPSS) version 22 was used to analyze data.Results: Out of 118 patients, 72% (n=85) were males while 28% (n=33) were females. Cultures of 105 patients were positive and most common organisms isolated were S. aureus (32.4%; n=34), E. coli (17.1%; n=18), P. aeruginosa (14.3%; n=15), Polymicrobials (14.3%; n=15) and K. pneumoniae (7.6%; n=8). Antibiotics to which organisms were most sensitive included Piperacillin/Tazobactam (69.5%; n=73), Imipenem (55.2%; n=58), Amikacin (43.8%; n=46), Vancomycin (40%; n=42) and Levofloxacin (38.1%; n=40).Conclusion: Most common organisms causing diabetic foot infections in our study were S. aureus, E. coli, P. aeruginosa, Polymicrobial and Klebsiella. Overall most sensitive antibiotics to these organisms included Piperacillin/Tazobactam, Imipenem,Amikacin, Vancomycin and Levofloxacin.

scholarly journals Spectral and Antibiotic Susceptibility of Pathogens Isolated from Saudi Patients with Diabetic Foot Infections

2021 ◽  
Vol 12 (1) ◽  
pp. 16-20
Author(s):  
Samiah Hamad S Al-Mijalli
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Foot ◽  
Antibiotic Susceptibility ◽  
Empirical Therapy ◽  
Multidrug Resistant ◽  
Foot Ulcers ◽  
Sensitivity Testing ◽  
Cross Sectional ◽  
Diabetic Foot Infections ◽  
Microbiological Methods

Diabetic foot infections (DFIs) are a significant health issue and a common complication among patients with diabetes. To develop antibiotic therapy for these high-risk patients, the current study evaluates the scope of DFIs and identifies the causing microbes. It also measures spectrum and antibiotic susceptibility of the pathogens isolated from adults with DFIs in Saudi Arabia. To achieve the study objectives, a cross-sectional study was implemented and the baseline characteristics for 44 patients with DFIs were defined. Optimal aerobic and anaerobic microbiological techniques were utilized to culture specimens isolated from infected foot ulcers. The standard microbiological methods were employed to identify the bacterial isolates and antibiotic susceptibility testing was conducted following the procedures of the Clinical and Laboratory Standards Institute (CLSI). Results showed that 12 microorganisms were isolated from the participants’ diabetic foot ulcers. Staphylococcus Aureus was ranked first because it appeared in 29 (65.9%) cases. Streptococcus Agalactiae was ranked second and multi-microbial infections were also found. Most of the organisms were susceptible to Vancomycin, Ciprofloxacin, and Cefalexin, but they were resistant to Methicillin, Gentamicin, and Ampicillin antibiotics. Staphylococcus Aureus was most sensitive to Ciprofloxacin, while it was resistant to Methicillin. About 10% of the isolates were multidrug-resistant. The study concludes that while Vancomycin should be used empirically for Gram-positive isolates, Ciprofloxacin can be taken into consideration for most of the Gram-negatives aerobes. Based on including various microorganisms and the advent of multidrug-resistant strains, proper culture and sensitivity testing are necessary prior to the empirical therapy.

scholarly journals MICROBIAL CONTENT RECOVERED FROM DIABETIC FOOT INFECTIONS: A CROSS-SECTIONAL STUDY IN BRAZIL / CONTEÚDO MICROBIANO RECUPERADO EM INFECÇÕES DE PÉ DIABÉTICO: UM ESTUDO TRANSVERSAL NO BRASIL

2020 ◽  
Vol 6 (10) ◽  
pp. 76209-76222
Author(s):  
Renata Barreto Francisco ◽  
Cristina Dutra Vieira ◽  
Luiz de Macêdo Farias ◽  
Antônio Eustáquio de Souza ◽  
Bruno Lima de Castro ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Diabetic Foot Infections

scholarly journals Antimicrobial Sensitivity Profile Of Uropathogens In A Macapá Laboratory, Amapá, Brazilian Amazon

2021 ◽  
pp. 81-102
Author(s):  
Ítalo Fernando Penha da Silva ◽  
José Maria Henriques Serruya Júnior ◽  
Tayonara Borges Gonçalves Góes ◽  
Bruno Gomes de Noronha ◽  
Cláudio Alberto Gellis de Mattos Dias ◽  
...  
Keyword(s):  
Protective Factor ◽  
Age Groups ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Female Population ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Private Laboratory ◽  
Predominant Pathogen ◽  
Tract Infections

Urinary tract infections (UTI) represent a worldwide health problem. Microbial resistance, due to selective antibiotic pressure, has a direct influence on the evolution and impact of these infections. The objective of this work was to identify the antimicrobial susceptibility profile of uropathogens isolated in uroculture samples from a private laboratory in the city of Macapá/AP. It is a quantitative, retrospective and cross-sectional study, using a laboratory database. Data were collected from results of urocultures with antibiogram analyzed from January to December 2019. The present study evaluated 3,510 urocultures, carried out during the study period, of which 1,269 had bacterial growth, equivalent to 36.15%. Among the positive results, we found the bacteria Escherichia coli (66.59%), Staphylococcus aureus (32.62%), mixed colonies of E. coli and S. aureus (0.47%), Staphylococcus saprophyticus (0.24%) and Serratia marcescens (0.08%). The male gender was responsible for 16.35% (n = 574) of the urocultures analyzed, while the female population was 83.65% (n = 2936). When analyzing the distribution of bacteria isolated by gender, males were considered a protective factor with 42% less chance of presenting bacteria in urine. The bacteria E. coli was the predominant pathogen in these infections in both sexes and in all age groups.

Study of bacterial infection and antimicrobial sensitivity pattern in diabetic foot lesion

2012 ◽  
Vol 6 (2) ◽  
pp. 20-23
Author(s):  
Shameem Akhter ◽  
Shah Md Zahurul Haque Asna ◽  
M Mushfequr Rahman ◽  
MA Hafez
Keyword(s):  
Diabetic Foot ◽  
Cross Sectional Study ◽  
Microbial Pathogens ◽  
Cross Sectional ◽  
Female Ratio ◽  
Antimicrobial Susceptibility Pattern ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Foot Lesions ◽  
Diabetic Foot Lesions

A cross sectional study was carried out on patients with diabetic foot lesions to determine the spectrum of aerobic microbial flora and to determine the microbial pathogens of the diabetic foot lesions and their antimicrobial sensitivity pattern. A total of 226 organisms were isolated from 218 patients and polymicrobial infection was found in 3.7 % cases. Age of the study population ranged from 20 - >80 yrs of which most of the patients were from 40 to 70 yrs (81 %). Male female ratio was= 3:1. In this study, Pseudomonas sp. (22.1 %), Proteus mirabilis (16.4 %) and S. aureus (14.6 %) were the predominant organisms isolated. Antimicrobial susceptibility pattern of the isolates were done in which imipenem and ciprofloxacin were found to be the most effective against all organisms.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19372 Bangladesh J Med Microbiol 2012; 06(02): 20-23

scholarly journals Surgical repair of Vesico-Vaginal: Vaginal route versus abdominal route.

2021 ◽  
Vol 28 (11) ◽  
pp. 1650-1655
Author(s):  
Abdul Qayoom Khuro ◽  
Illahi Bux Brohi ◽  
Muhammad Shahid Bhatti
Keyword(s):  
Success Rate ◽  
Sampling Technique ◽  
Cross Sectional Study ◽  
Medical Sciences ◽  
Vaginal Route ◽  
Fistula Repair ◽  
Cross Sectional ◽  
Abdominal Route ◽  
Expert Surgeon ◽  
Transvaginal Route

Objective: To determine outcomes and complications of vesico-vaginal fistula repair via vaginal and abdominal route. Study Design: Cross Sectional study. Setting: Department of Urology and Kidney Transplantation, Pir Abdul Qadir Institute of Medical Sciences Gambat. Period: January 2020 to December 2020. Material & Methods: Patients having vesicovaginal fistula irrespective of age and fulfilling our inclusion criteria were included in the study using non-probability consecutive sampling technique. Patients having very large fistula, fistula involving neck of urinary bladder, failure of previous operation, patients with malignancy or co-morbidities were excluded from the study. Vaginal and abdominal approaches were used for fistula repair depending upon the level of fistula. Those operated via vaginal route were kept in trans-vaginal group and those operated via abdominal route were assigned trans-abdominal group. Results: Total 35 cases were studied having age 16-45 years with mean age of 32.6 ± 4.2 years. Transvaginal fistula repair was done in 37.1% and transabdominal repair was done in 62.8% cases. Most common cause of VVF was previous gynecological surgery in 77% cases. Success rate was 100% in transvaginal fistula repair as compared to 95.4% success rate achieved in transabdominal repair of fistula. Conclusion: Trans abdominal and transvaginal route both are good approaches but Transvaginal route of fistula repair is associated with high success rate than transabdominal route with minimum complications and better outcomes but it needs expert surgeon.

scholarly journals Bacteriological study of nidus of gallstones

2019 ◽  
Vol 6 (9) ◽  
pp. 3271
Author(s):  
Christo Cyriac Thomas ◽  
Sreejith V. ◽  
Rosna Mary Anto ◽  
Fleming Nagarajan ◽  
Debaleena Goswami ◽  
...  
Keyword(s):  
Microbial Growth ◽  
Gallstone Disease ◽  
Gall Stone ◽  
Cross Sectional Study ◽  
Medical Sciences ◽  
Cross Sectional ◽  
Bacteriological Study ◽  
Gold Standard Method ◽  
E Coli ◽  
Infective Potential

Background: Gallstone disease is common worldwide and majority of patients undergo surgical management. Since the advent of laparoscopic cholecystectomy surgeons has become less interested in its aetiology. Infection is a major factor in the formation of gallstones. There are many infective complications reported of gallstones spilled during cholecystectomy. Presence of bacteria or its DNA by microscopy does not prove the infective potential of such stones. Thus culture of the nidus of the gallstone is the gold standard method to identify any potentially infective bacteria within and to predict the infective potential of gallstones.Methods: Cross sectional study done in seventy patients admitted for elective cholecystectomy for a period of 24 months from August 2016 to July 2018 in Department of Surgery, Regional Institute of Medical Sciences, Imphal, India.Results: Of the 70 stones cultured for bacteria, 52 (74.3%) were sterile and 18 (25.7%) were positive for bacteria, among which 16 stones showed mono-microbial growth while 2 showed poly-microbial growth. Most commonly isolated organism was E. coli and Klebsiella followed by Pseudomonas, Enterococcus and Staph aureus.Conclusions: Moynihan‘s aphorism “gall stone is a tomb erected in the memory of the organism within it”, suggest bacteria inside gallstone to be dead but this study proves that bacteria can be viable inside gallstone and its complete retrieval should be done in case of spillage which may help in avoiding complications. 

scholarly journals Prevalence of anti-herpes simplex virus type 1 among dental students of Birjand University of Medical Sciences, Iran, 2017-2018: Short Communication

2021 ◽  
pp. 296-301
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Close Contact ◽  
Cross Sectional Study ◽  
Dental Students ◽  
Enzyme Linked Immunosorbent Assay ◽  
Medical Sciences ◽  
Cross Sectional ◽  
Simplex Virus

Herpes simplex virus is considered one of the most common human infections in most parts of the world, among which, herpes simplex virus 1 (HSV-1) is one of the most important human pathogenic viruses that cause numerous skin and oral lesions. Practitioners of the dental profession and related fields are in close contact with numerous patients referring to dental clinics and are at risk of cross-infection. Regarding this, one of the infections that dental professionals are exposed to is herpes simplex virus infection. This study aimed to evaluate the level of HSV1 antibody among dental students of Birjand in 2018-2019. This cross-sectional study was performed on 100 dental students of Birjand University of Medical Sciences, Iran. After obtaining informed consent, a peripheral blood sample (5 ml) was collected from participants and the presence of anti-virus antibody was examined using an Anti-Herpes-1 IgG kit with the enzyme-linked immunosorbent assay. It was revealed that 41% of the subjects had anti-herpes type 1 antibodies. Based on data analysis, the prevalence of HSV1 antibody was higher in pre-clinical and clinical level than in basic science; however, there was no significant relationship between students' educational level and antibody prevalence.

“Evaluation of Urosepsis and Bacteriuria in Patients Undergoing PCNL and URS”

2021 ◽  
Vol 8 (09) ◽  
pp. 5584-5590
Author(s):  
Bivash Boran Biswas ◽  
Mousumi Malakar ◽  
Sazzad Bin Shahid ◽  
Sayem Hossain ◽  
Khan Nazrul Islam ◽  
...  
Keyword(s):  
Blood Culture ◽  
Urine Culture ◽  
Sampling Technique ◽  
Cross Sectional Study ◽  
Resistant Organism ◽  
College Hospital ◽  
Cross Sectional ◽  
E Coli ◽  
Medical College ◽  
Sensitivity Pattern

Urosepsis usually develops from a community or nosocomial acquired urinary tract infection (UTI) or during the procedure of various urinary disease such ureterorenoscopy (URS) and percutaneous nephrolithotomy (PCNL). Urosepsis is associated with bacteriuria, Urosepsis due to manipulation during or after percuteneous nephrolithotomy (PCNL) or ureterorenoscopy (URS) or push bang stenting can be catastrophic despite prophylactie antibiotic coverage. This cross sectional study was carried out in Dhaka Medical College Hospital, Dhaka, Bangladesh during the period of July 2011 to June 2013. Sampling technique was purposive and sample size was 70. Among them 23 patients for PCNL and 47 patients for URS were selected by selection criteria. Data were collected by interview of the patients, clinical examinations and laboratory investigations using the research instrument. Data were processed and analyzed using software SPSS (Statistical Package for Social Sciences) version 11.5. Incidence of bacteriuria and urosepsis were measured according to urine and blood culture report. Sensitivity pattern was also observed. According to this study, the incidence of bacteriuria and urosepsis were 17.1% and 5.7% respectively, Of the 70 patients, 12(17.1%) exhibited bacterial growth on urine culture, These 12 patients were then subjected to blood culture and 4(33.3%) of them were found positive. Most (83.4%) of the urine and blood infections (75%) were caused by E. coli. Some widely used antibiotics like moxicillin, Cephalexin and Ciprofloxacin were found 100% resistant in urine culture. Few sensitive antibiotics were Tobramycin (100 %), Amikacin and Ceftazidime (75%). Almost same sensitivity pattern was found in blood culture. In urosepsis, as in other types of sepsis. Urosepsis after PCNL and URS is an important and potentially catastrophic complication. Percuteneous nephrolithotomy (PCNL), Ureterorenoscopy (URS) occurs frequently in this institution. Although the incidence of urosepsis and bacteriuria with resistant organism is low, but it is a burning issue in management in urology practice. The apparent increase in ciprofloxacin resistant organisms appears to be associated with the increased rate of ciprofloxacin resistant organisms are observed in the general population.

scholarly journals Correlation between superficial and intra-operative specimens in diabetic foot infections: results of a cross-sectional Tunisian study

2019 ◽  
Vol 19 (3) ◽  
pp. 2505-2514 ◽  
Author(s):  
Foued Bellazreg ◽  
Ahmed Guigua ◽  
Asma Ferjani ◽  
Zouhour Hattab ◽  
Jalel Boukadida ◽  
...  
Keyword(s):  
Antibiotic Therapy ◽  
Diabetic Foot ◽  
Susceptibility Testing ◽  
Cross Sectional Study ◽  
Drug Resistant ◽  
Sectional Study ◽  
Cross Sectional ◽  
Diabetic Foot Infections ◽  
And Staphylococcus Aureus ◽  
Extremity Amputation

Objective: To determine the correlation between superficial, and intra-operative specimens in diabetic foot infections (DFIs).Methods: We conducted a cross-sectional study in patients with DFIs hospitalized in a Tunisian teaching hospital. Superficial specimens were collected for all patients, and intra-operative specimens were collected in operated patients. The specimens were processed using standard microbiology techniques. Antimicrobial susceptibility testing was carried out according to the protocol established by the European Committee on Anti-microbial Susceptibility Testing. Intra-operative and superficial specimens were considered correlated if they isolated the same microorganism(s), or if they were both negative.Results: One hundred twelve patients, 81 males and 31 females, mean age 56 years, were included. Superficial samples were positive in 77% of cases, and isolated 126 microorganisms. Among the positive samples, 71% were monomicrobial. The most frequently isolated microorganisms were Enterobacteriaceae (53%), followed by streptococci (21%) and Staphylococcus aureus (17%). Nine microorganisms (7%) were multi-drug resistant. Intra-operative samples were positive in 93% of cases. Superficial specimens were correlated to intra-operative specimens in 67% of cases. Initial antibiotic therapy was appropriate in 70% of cases. The lower-extremity amputation and the mortality rates were 41% and 1%, respectively.Conclusion: In our study, DFIs were most frequently caused by Enterobacteriaceae and superficial specimens were correlated to intra-operative specimens in only two thirds of cases. Clinicians should emphasize on the systematic practice of intraoperative specimens in all patients with DFIs treated surgically, while well-performed superficial specimens could be useful for prescribing appropriate antibiotic therapy in other patients.Keywords: Diabetic foot infections, superficial specimens, intra-operative specimens.

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