scholarly journals Kadar Kolesterol Plasma Tikus Wistar pada Pemberiak Ekstrak Etanol dan Heksana dari Daun Gedi Merah (Abelmoschus manihot L.)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 63
Author(s):  
Tanza A. Tubagus
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Plasma Cholesterol ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Amperometric Biosensor ◽  
Male Rats ◽  
Measuring Instrument ◽  
Cholesterol Levels ◽  
One Way Anova

Telah dilakukan penelitian untuk mempelajari pengaruh pemberian ekstrak etanol dan heksana dari daun gedi merah terhadap kadar kolesterol plasma tikus wistar yang diberi pakan aterogenik. Penelitian ini menggunakan 20 ekor tikus jantan, berumur 2-3 bulan dengan berat 130-200 g, yang dibagi dalam 4 kelompok perlakuan secara acak, yaitu kelompok K diberi pakan beras jagung, kelompok K- diberi pakan aterogenik, sedangkan kelompok P1 dan P2 diberi ekstrak etanol dan heksana dengan dosis 20 mg/KgBB. Setiap perlakuan terdiri dari 5 ekor tikus yang dilakukan 5 kali pemeriksaan kadar kolesterol dengan metode biosensor amperometrik dengan menggunakan alat ukur NESCO GCU. Data yang diperoleh dari pemeriksaan dianalisis menggunakan ANOVA satu arah <(0,005). Hasil penelitian menunjukkan bahwa ekstrak heksana dan ekstrak etanol daun gedi merah dengan dosis 20 mg/kgBB dapat menurunkan kadar kolesterol sebesar 72% atau sekitar 12,8 mg/dL dan 14% atau sekitar 2,4 mg/dL, dibandingkan dengan dengan kelompok K-.The research had been done with the purpose were to study the influence of granting the ethanol and hexane extract of red gedi leaves on Wistar rats plasma cholesterol levels which were fed by atherogenics. This study used about 20 male rats, Wistar rats aged 2-3 months with a weight 130-200 g were divided into 4 groups randomly, for the grop of K were fed corn rice, group of K- were fed atherogenics, while the P1 and P2 were given ethanol and hexane extract with a dose of 20 mg/KgBW. Each treatment consisted of 5 rats were performed 5 times cholesterol checks with amperometric biosensor method using a measuring instrument NESCO GCU. Data obtained from the examination were analyzed using one-way ANOVA <(0.005). The results showed that the hexane extract and red gedi leaf ethanol extract at a dose of 20 mg / kg body weight can low down the cholesterol levels by 72% or approximately 12.8 mg / dL and 14%, or about 2.4 mg / dL, compared with the group K -.

scholarly journals Uji Aktivitas Antikolesterol Ekstrak Etanol Daun Patikan Emas (Euphorbia prunifolia Jacq.) pada Tikus Wistar yang Hiperkolesterolemia

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 29
Author(s):  
Nadhilah Bachmid
Keyword(s):  
Wistar Rats ◽  
Plasma Cholesterol ◽  
Ethanol Extract ◽  
Amperometric Biosensor ◽  
Measuring Instrument ◽  
Cholesterol Levels ◽  
Male Wistar Rats ◽  
One Way Anova

Telah dilakukan penelitian untuk menentukan aktivitas antikolesterol ekstrak etanol daun patikan emas pada tikus wistar yang hiperkolesterolemia. Tikus wistar jantan yang berusia 2-3 bulan sebanyak 20 ekor dibagi menjadi 4 kelompok perlakuan secara acak. Kelompok K diberi pakan beras jagung, sedangkan kelompok K-, P1, dan P2 diberi pakan aterogenik. Kelompok P1 dan P2 diberi ekstrak dengan dosis 10 dan 30 mg/kgBB. Pemeriksaan kadar kolesterol plasma ditentukan dengan metode biosensor amperometrik menggunakan alat ukur NESCO GCU dengan bantuan strip yang telah ditetesi darah. Darah tikus diperoleh dari bagian ekor yang telah dilukai. Data yang diperoleh dianalisis menggunakan ANOVA satu arah (p<0,05). Hasil penelitian menunjukkan ekstrak etanol daun patikan emas dengan dosis 10 dan 30 mg/kgBB memiliki aktivitas antikolesterol dengan penurunan kadar kolesterol sebesar 12 dan 71% dibandingkan dengan kelompok K-.Research had been carried out to determine anticholesterol activity of ethanol extract gold patikan leaves. 20 Male wistar rats aged 2-3 months were divided into 4 groups randomly. K group with corn rice fed, while the K-, P1 and P2 with atherogenic fed. Group P1 and P2 are given with extract with doses 10 and 30 mg/kgBW. Examination of plasma cholesterol levels were determined by amperometric biosensor methods using a measuring instrument NESCO GCU with strip that had spilled blood. Blood was obtained from rats tail that had been injured. Data were analyzed using one-way ANOVA (p<0,05). The results showed that the ethanol extract of gold patikan leaves with doses 10 and 30 mg/kgBW decrease the anticholesterol activity with cholesterol levels by 12 and 71%, compared with K- group.

scholarly journals Aktivitas Antihiperglikemik dari Ekstrak Etanol dan Heksana Tumbuhan Suruhan (Peperomia pellucida [L.] Kunth) pada Tikus Wistar (Rattus norvegicus L.) yang Hiperglikemik

Jurnal MIPA ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 109
Author(s):  
Sariyana Togubu ◽  
Lidya I. Momuat ◽  
Jessy E. Paendong ◽  
Navila Salma
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Tolerance Test ◽  
Negative Control ◽  
Hexane Extract ◽  
Test Method

Telah dilakukan penelitian kadar glukosa darah tikus wistar yang hiperglikemia pada pemberian ekstrak etanol dan heksana tumbuhan suruhan (Peperomia pellucida [L]. Kunth). Penelitian ini menggunakan metode uji toleransi glukosa, terhadap 16 ekor tikus jantan wistar yang hiperglikemia akibat diinduksi sukrosa. Selanjutnya, tikus dibagi dalam 4 kelompok perlakuan yaitu kelompok kontrol negatif (K-) diberi CMC 0,5%, kelompok kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok ekstrak etanol tumbuhan suruhan (EETS) dosis 40 mg/kgBB, dan kelompok ekstrak heksana tumbuhan suruhan (EHTS) dosis 40 mg/kgBB. Setiap kelompok terdiri dari 4 ekor tikus. Kadar glukosa darah tikus diukur dengan alat Drglukometer pada menit ke-30, 60, dan 120 setelah diberikan perlakuan. Hasil penelitian menunjukkan bahwa pada menit ke-120, kadar glukosa darah tikus pada kelompok EETS dan EHTS masing-masing turun sebesar 54,57% dan 51,25%, dan tidak berbeda nyata dengan K(+). Kadar glukosa darah kelompok K(+) dan EETS pada menit ke-120 mencapai kadar yang sama dengan keadaan basal (normal). Penelitian ini menyimpulkan bahwa ekstrak etanol dan heksana tumbuhan suruhan dengan dosis 40 mg/KgBB memiliki efek hipoglikemik pada tikus jantan wistar yang hiperglikemia.A study on blood glucose level in hyperglycemic wistar rats treated with ethanol and hexane extracts of suruhan (Peperomia pellucida [L]. Kunth) had been done. This study used glucose tolerance test method applied on 16 male wistar rats which were hyperglycemic by sucrose induction. The rats were divided into four treatment groups, each of which contained four rats, which were negative control (K-) group treated with CMC 0.5%, positive control (K+) group treated with glibenclamide 0.45 mg/kg body weight, group treated with ethanol extract of P. pellucida (EETS) 40 mg/kg body weight, and group treated with hexane extract of P. pellucida (EHTS) 40 mg/kg body weight. Glucose level in rat blood was measured using Drglucometer at minute 30, 60, and 120 after treatment. The results showed that at minute 120 the blood glucose level of EETS and EHTS was decreased by 54.57% and 51.25%, respectively, which were not significantly different from K(+). At minute 120, blood glucose of K(+) and EETS reached the same level with that of basal (normal) state. It was concluded that ethanol and hexane extract of P. pellucida 40 mg/kg body weight had hypoglycemic effect on hyperglycemic wistar rats.

scholarly journals Effect of Seaweed Ethanol Extract on Cholesterol Levels and Obesity in White Rats

2020 ◽  
Vol 5 (3) ◽  
pp. 98-105
Author(s):  
Magfirah ◽  
Indah Kurnia Utami ◽  
Syafika Alaydrus
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Plasma Cholesterol ◽  
Ethanol Extract ◽  
Food Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Obese Rats ◽  
Pap Method ◽  
Marine Areas

Seaweed (EucheumaCottonii J. Agardh) is one of the biological resources found in coastal and marine areas. Seaweed contains secondary metabolites such as flavonoids, alkaloids, polyphenols and tannins which can reduce cholesterol levels in the blood. This study aims to determine the effect of seaweed ethanol extract on reducing cholesterol and obesity levels by comparing plasma cholesterol levels, body weight, and weight of vital organs (liver, kidneys and jantumg) of obese rats, mice given seaweed ethanol extract with normal mice . Rats were grouped randomly into 6 groups. group one was given 0.5% CMC Na, group two was given high fat diet food, group three was given simvastatin, group four was given seaweed ethanol extract 100 mg / kgbb, group five was given ethanol extract of seaweed 200 mg / kgbb and group six was given ethanol extract of seaweed 300 mg / kg with a long suspension of extract for 14 days. Measurement of cholesterol levels in the blood using the CHOD-PAP method, body weight and organs carried out by weighing. The results showed that seaweed ethanol extract has the effect of inhibiting an increase in plasma cholesterol levels and has anti-obesity potential at a dose of 300 mg / kgbb.

scholarly journals HYPOGLYCEMIC AND HYPOLIPIDEMIC EFFECTS OF ANNONA MURICATA L. LEAF ETHANOL EXTRACT

2017 ◽  
Vol 9 (3) ◽  
pp. 170 ◽  
Author(s):  
E. Sovia ◽  
W. Ratwita ◽  
D. Wijayanti ◽  
D. R. Novianty
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ethanol Extract ◽  
Normal Group ◽  
Islet Of Langerhans ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Annona Muricata ◽  
Cholesterol Levels

Objective: The aim of this study was to investigate hypoglycemic and hypolipidemic effects of Annona muricata leaf ethanol extract. This study also investigated phytochemical analysis of the extract and improvement in the islet of Langerhans.Methods: Hypoglycemic and hypolipidemic effects were evaluated using alloxan induced diabetic rats. Twenty-five Wistar rats devided into five groups, that are normal group, control group that induced by alloxan 125 mg/kg body weight and given CMC (carboxymethyl cellulose), and three treatment group that induced by alloxan and given Annona muricata leaf ethanol extract (AMLEE) with 50, 100 and 200 mg/kg body weight. Blood glucose and total cholesterol levels were measured before and after alloxan induction, and 21 d after AMLEE treatment. At the end of the study, all of the animals experiment were sacrificed for histopathological examination.Results: Phytochemical analysis revealed that flavonoids, tannins, saponins, phytosterols and phenols were present in ethanol extract of Annona muricata L. leaf. At the end of the study, blood glucose and total cholesterol levels in all AMLEE treated group were decreased significantly (p<0.05). Final blood glucose level in the groups that given AMLEE 50 mg/kg body weight (86.7±14.6 mg/dl) was almost the same with that of the normal group (91.25±28.38 mg/dl). The result of the histopathological examination is not showed an improvement of the islet of Langerhans in AMLEE treated groups.Conclusion: In conclusion, the ethanol extract of Annona muricata L. leaf have hypoglycemic and hypolipidemic effects. However, there was no improvement in the islet of Langerhans damage.

scholarly journals Characterization of apolipoprotein E-deficient rats as novel model for atherosclerosis imaging

2021 ◽  
Vol 22 (Supplement_3) ◽  
Author(s):  
JWA Sijbesma ◽  
A Van Waarde ◽  
S Kristensen ◽  
I Kion ◽  
UJF Tietge ◽  
...  
Keyword(s):  
Body Weight ◽  
Apolipoprotein E ◽  
Aortic Arch ◽  
Abdominal Aorta ◽  
Pet Imaging ◽  
Plasma Cholesterol ◽  
Preclinical Model ◽  
Aortic Tissue ◽  
Cholesterol Levels ◽  
Positron Emission

Abstract Funding Acknowledgements Type of funding sources: None. Background The apolipoprotein E-deficient (apoE-/-) mouse is a well-established atherosclerotic model with impaired lipoprotein clearance and development of vessel plaques. However, the small size of the mouse limits its use as an animal model in longitudinal positron emission tomography (PET) imaging studies of atherosclerosis. Recently, apoE-/- rats have become available. This study addresses the suitability of the apoE-/- rat as model for atherosclerotic PET imaging. Methods Ten male apoE-/- rats and ten male control rats (apoE+/+) (age 10+/-1 weeks), each fed with a Western diet, were injected at baseline week 4, 12, 26 and 51, with 60 MBq of [18F]2-fluoro-2-deoxy-D-glucose. Plasma cholesterol, body weight and fat were measured. 3h after injection, a computed tomography (CT) and a 20-min PET scan were made. After the final scan, aortic tissue was collected for histological staining. Results Cholesterol levels started to increase after 4 weeks in the apoE-/- rats, whereas in the apoE+/+ rats levels stayed stable. Body weight and body fat increased more rapidly in the apoE-/- rats but were similar in both strains at the end of the study. SUVmean and max in the aortic arch and abdominal aorta were significantly higher (p &lt; 0.001) in apoE-/- versus apoE+/+ rats at weeks 12, 26 and 51. Oil red O staining showed lesions in 20.0 % of the surface of the aortic arch in the apoE-/- rats. Hardly no fatty streaks were detected in the apoE+/+ rats (2.29%). More histology data is being analyzed. Conclusion Plasma cholesterol levels were elevated in apoE-/- rats. PET imaging demonstrated differences in [18F]-FDG uptake in the aortic arch and abdominal aorta after 12, 26 and 51 weeks. Combined, these data demonstrate that apoE-/- rats represent a useful preclinical model for the non-invasive assessment of atherosclerosis in longitudinal studies.

scholarly journals Effect of Basil Seed and Chia SeedExtracts on Blood Lipid Profile

2021 ◽  
Vol 15 (6) ◽  
pp. 1368-1371
Author(s):  
S. Munir ◽  
S. Khurshid ◽  
Q. J. Iqbal ◽  
N. Iqbal ◽  
Z. Masood
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Plasma Cholesterol ◽  
Plasma Lipid ◽  
Blood Lipid ◽  
Histopathological Analysis ◽  
Omega 3 ◽  
Blood Lipid Profile ◽  
Cholesterol Levels ◽  
Maintain Body Weight

Background: Basil and Chia seeds contain higher nutritive values like vitamin, carbohydrates, Omega-3 oil and other dietary fibers. With all these rich dietary benefits these seeds regulate necessary health conditions and maintain body weight. Ocimumbasilicum (Basil) plant have been known to contain properties of weight loss, better digestion and other health benefits. Aim: To check the Basil seed against hyperlipidemia in mice with Chia seeds. Methodology: In this research, the effect of both seeds extract on body weight and plasma lipid profile were estimated in Albino mice after raising their cholesterol levels by high fatty diet. The experiments were performed in different groups like normal control, standard control, hyperlipidemia group and four groups of diet supplemented chia or basil seeds with two different doses. Results: The biochemical analysis revealed that the supplementation of Basil seeds (400mg/kg/day) significantly lowered the levels of total plasma cholesterol, lipoproteins and triacylglycerol. Moreover, histopathological analysis of vital organs like kidneys, heart reported no toxicity. Conclusion: Extracts of Chia and Basil seeds have shown controlling effects over the given parameters in the blood and weights of the animals and these may have potential to control high fat diet-induced hyperlipidemia when taken as dietary supplements. Keywords: Cholesterol, Hyperlipidemia, Ocimumbasilicum, Salvia hispanica

Hypothalamic paraventricular nucleus lesions do not prevent anorectic effect of exercise in male rats

1990 ◽  
Vol 259 (3) ◽  
pp. R579-R584 ◽  
Author(s):  
S. Rivest ◽  
D. Richard
Keyword(s):  
Body Weight ◽  
Energy Balance ◽  
Paraventricular Nucleus ◽  
Wistar Rats ◽  
Energy Gain ◽  
Hypothalamic Paraventricular Nucleus ◽  
Male Rats ◽  
Hypothalamic Nucleus ◽  
Serum Corticosterone ◽  
Male Wistar Rats

The effects of a hypothalamic paraventricular nucleus (PVN) lesion on energy balance were investigated in exercise-trained rats. Male Wistar rats weighing initially 250 g were divided into four groups. Two groups of rats underwent a bilateral PVN lesion, whereas the two remaining groups were sham operated. The PVN lesions were done electrolytically. One group from each surgical treatment was exercised, while the other group was kept in sedentary conditions. Rats were exercised on a rodent motor-driven treadmill at moderate intensity, 1 h/day for 21 consecutive days. Food intake and body weight were measured each day during the study. At the end of the treatment period, rats were killed, and carcasses were analyzed for their energy content. Serum corticosterone was measured by a competitive protein-binding assay. Energy gain and energy intake were lower in exercised rats than in sedentary controls, regardless of whether they were sham or PVN lesioned. Concurrently, there was no difference in the energy gain between PVN-lesioned and sham-operated rats, despite the fact that PVN-lesioned rats ended the experiment with a larger body weight than the sham-lesioned animals. Serum corticosterone levels were lower in PVN-lesioned rats than in sham-lesioned rats. In conclusion, the present results indicate that the PVN, the hypothalamic nucleus predominantly controlling the pituitary-adrenal axis activity, is not a prominent structure in the regulation of energy balance in exercised male Wistar rats.

EFFECTS OF AMPHENONE B AND OF DIET ON CHOLESTEROL LEVELS IN THE RAT

1959 ◽  
Vol 37 (1) ◽  
pp. 1069-1074 ◽  
Author(s):  
K. K. Carroll
Keyword(s):  
Erucic Acid ◽  
Plasma Cholesterol ◽  
Young Male ◽  
Cholesterol Content ◽  
Male Rats ◽  
Adrenal Weight ◽  
Chow Diet ◽  
Cholesterol Levels ◽  
High Doses

Amphenone B was added in concentrations of 0.1 to 0.5% to a number of synthetic diets and to a fox chow diet. These were then fed to young male rats to determine the effects on adrenal weight and on the cholesterol content of the adrenals, liver, and plasma. The rats fed amphenone mixed with synthetic diets showed a greater increase in adrenal size and adrenal cholesterol than those fed amphenone mixed with the chow diet. The liver and plasma cholesterol values of rats fed amphenone on synthetic diets were also increased appreciably while those of rats fed amphenone in the chow diet were near normal. Synthetic diets containing erucic acid and amphenone appeared to have the greatest effect on adrenal size and cholesterol content but other synthetic diets in some cases caused a greater increase in liver and plasma cholesterol concentrations. An attempt to examine the effect of injected amphenone on cholesterol levels in rats fed different diets was limited by the fact that high doses of amphenone cause prolonged anesthesia.

scholarly journals Lactobacillus Rhamnosus Yoba Modulates Serum Cholesterol in Normo-Lipidemic Wistar Rats Fed a High Fat Diet (P20-013-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Adaku Iwueke ◽  
Conrad Miruka ◽  
John Ejekwumadu ◽  
Ronald Kiiza ◽  
Pius Theophilus
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Lactobacillus Rhamnosus ◽  
Hdl Cholesterol ◽  
Male Rats ◽  
Fermented Foods ◽  
Distilled Water ◽  
High Fat

Abstract Objectives The study was aimed at assessing the effects of Lactobacillus rhamnosus yoba on the serum lipid profile and body weight of male Wistar rats fed a high fat diet. Methods 20 seven week old male rats weighing between 120 g and 180 g were used for the study and divided into 4 groups of 5 rats each. The control group was fed normal mice pellets and distilled water, while the other groups were fed mice pellets supplemented with 3% cholesterol and 2% saturated fat in addition to any of distilled water, Lactobacillus rhamnosus yoba or Norvastatin respectively. The body weight was measured at the start of the study and after 2 weeks while serum parameters were measured after 8 weeks. Data was analyzed using SPSS Version 20. ANOVA and Tukey's tests determined significant differences in means at 95% confidence interval. Results Lactobacillus rhamnosus yoba significantly (P < 0.005) modulated weight gain, serum total cholesterol and triglycerides when compared to the control. Similarly, LDL-cholesterol was significantly modulated (P < 0.005) while HDL-cholesterol was significantly enhanced (P < 0.005) when compared to the control. Conclusions The ability of Lactobacillus rhamnosus yoba to elevate HDL cholesterol and modulate LDL-cholesterol without the side effects of statins makes it a potential functional food. In line with the findings, the present study justifies the use of Lactobacillus rhamnosus yoba as a probiotic in fermented foods. Funding Sources NA.

scholarly journals The Efficacy of Seluang Fish Oil (Rasbora agrotynea) Related Body Weight, Lipid Profile, Adiponectin and Leptin in Wistar Rats-Induced High Fat Diet

2018 ◽  
Vol 2 (2) ◽  
pp. 25-30
Author(s):  
Fatmawati Karim ◽  
Rachmat Hidayat ◽  
Erizka Rivani ◽  
Husnul Khotimah ◽  
Ester G Pansserga
Keyword(s):  
Body Weight ◽  
Fish Oil ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Adiponectin Level ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Reduce Body Weight

Background Rasbora agrotynea (local name : Seluang ) is a fish native to Sumatera, Malaya and Borneo. This fish has a potential as a local wisdom for supplementation of omega 3 and omega 6. In Sumatera, Seluang fish was used as traditional food.   Aim of Study Aim of this study to explore the efficacy of seluang fish oil in body weight, lipid profile, adiponectin and leptin level in Wistar Rats-Induced High Fat Diet   Methods This study was an experimental study , pre-post test with control group design. The sample in this study was  30 male rats, 8 weeks old, weight 150-200 gram. Rats were given high fat diet and seluang fish oil  at dose of 0,1 , 0,2  and 0,4 mL/200 gr BW/day for 2 weeks. Seluang fish oil was extracted by distilation methods. The results of this study were assayed by SPSS 18.   Results Seluang fish oil 0,4 mL/200 gr BW  was more potent to reduce body weight gain, triglyseride level,  leptin and increase adiponectin level than seluang fish oil 0,1 mL/200 gr BW, 0,2 mL/200 gr BW, negative control and positive control.   Conclusion Seluang fish oil had a potention to reduce body weight, triglyceride, leptin and increase adiponectin level.   Keywords: Seluang fish Oil – body weight- triglyserida – leptin - adiponectin  

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