scholarly journals TUMOR CELL ABUNDANCE IN CLASSIC HODGKIN LYMPHOMA

2019 ◽  
pp. 1-4
Author(s):  
Daniel Benharroch
Keyword(s):  
Tumor Cell ◽  
Cell Count ◽  
Hodgkin Lymphoma ◽  
Tumor Cells ◽  
Measles Virus ◽  
Indirect Evidence ◽  
Biological Properties ◽  
High Rate ◽  
Malignant Cells ◽  
Classic Hodgkin Lymphoma

OBJECTIVES: Study the relevance of a high proportion of tumor cells to the clinical and biological properties of classic Hodgkin lymphoma. METHODS: Tumor cells were counted in sections of the lymphoma stained with CD30,as this highlights the tumor cells of this neoplasm.We assessed abundant malignant cells (≥99 cells/10 high power fields) as the median count of tumor cells. RESULTS: A wealth of tumor cells was detected in 61 (52.1%) patients, in contrast with 56 (47.9%) patients with a low count.No clinical variance was found between cases rich or poor in malignant cells,except for a statistically significant preference of a high rate of tumor cells for female patients as well as for a high expression of measles virus antigens.The apoptotic tumor cell distribution showed no major disparity with the neoplasm characteristics. CONCLUSIONS: The entire type spectrum of Hodgkin lymphoma as evaluated by the tumor cell count has no direct bearing on the course of the lymphoma. But there might be indirect evidence for an association of a high tumor cell count,female preference,a strong expression of measles virus antigens and a poor prognosis

scholarly journals Formation of the Immunosuppressive Microenvironment of Classic Hodgkin Lymphoma and Therapeutic Approaches to Counter It

2019 ◽  
Vol 20 (10) ◽  
pp. 2416 ◽  
Author(s):  
Donatella Aldinucci ◽  
Cinzia Borghese ◽  
Naike Casagrande
Keyword(s):  
T Cell ◽  
Tumor Microenvironment ◽  
Hodgkin Lymphoma ◽  
Tumor Cells ◽  
Current Knowledge ◽  
Treatment Strategies ◽  
T Cell Exhaustion ◽  
Normal Cells ◽  
Cell Exhaustion ◽  
Classic Hodgkin Lymphoma

Classic Hodgkin lymphoma (cHL) is characterized by a few tumor cells surrounded by a protective, immunosuppressive tumor microenvironment composed of normal cells that are an active part of the disease. Hodgkin and Reed–Sternberg (HRS) cells evade the immune system through a variety of different mechanisms. They evade antitumor effector T cells and natural killer cells and promote T cell exhaustion. Using cytokines and extracellular vesicles, they recruit normal cells, induce their proliferation and “educate” (i.e. reprogram) them to become immunosuppressive and protumorigenic. Therefore, alternative treatment strategies are being developed to target not only tumor cells but also the tumor microenvironment. Here we summarize current knowledge on the ability of HRS cells to build their microenvironment and to educate normal cells to become immunosuppressive. We also describe therapeutic strategies to counteract formation of the tumor microenvironment and related processes leading to T cell exhaustion and repolarization of immunosuppressive tumor-associated macrophages.

scholarly journals STUDY OF EFFECTS OF CIRCADIAN RHYTHMS ON SOLID TUMOR CELLS PROLIFERATION AND CHEMORESISTANCE

2020 ◽  
Vol 66 (5) ◽  
pp. 563-571
Author(s):  
Anna Danilova ◽  
N. Avdonkina ◽  
Ye. Gubareva ◽  
I. Baldueva ◽  
Anton Zozulya ◽  
...  
Keyword(s):  
Circadian Rhythms ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Solid Tumor ◽  
Lung Tumor ◽  
Morphological Characteristics ◽  
Biological Properties ◽  
Physiological Processes

Circadian clock is a complex mechanism regulating many different physiological processes. Preclinical, epidemiological and clinical studies demonstrate association between circadian rhythms disruption and tumor initiation. Study of modulation of solid tumor cells biological properties through enhancement of clock mechanisms could attribute to the development of more effective chemo- and hormone therapy approaches. Aim: Evaluate the effects of ovarian and lung tumor cells synchronization with dexamethasone in vitro on cells sensitivity to cisplatin. Materials and methods: Metastatic ovarian cancer (n=3) and lung cancer (n=3) cell lines were obtained from patients tumors. Tumor cell cultivation was performed in accordance with the protocol. Artificial synchronization was performed with dexamethasone 200 nM introduction to the cell cultures. Doses of cisplatin used were 1.5 and 3.0 mg/ml. xCELLigence Real-Time Cell Analysis and Cell-IQ was used to measure proliferation and chemoresistance of tumor cells. Results: Each cell-line had individual morphological characteristics and proliferation parameters. Preliminary incubation with dexamethasone (2 h) had a stimulating effect on proliferation of all tumor cell lines (Slope min -4.3(0.3)хЕ ‘х10-3 - max 36.8(0.6)хЫх10'3, min 2.2(0.2)хЕ1х10'3- max 50.4(0.8)хЕ1х10'3), and increased their sensitivity to cisplatin (min -43(2.6)хЕ1х10-3 - max 57.5(0.6)хЕ1х10-3 и min -217,3(2,2) -1,9(0,1)хч-1х10-3 - max -1,9(0,1)хч'1х10'3, respectively. Conclusion: These results should be the platform for future studies of the interaction of clock mechanisms, cell cycle regulation and viability of tumor cells.

scholarly journals Is the Measles Virus Indeed Involved in Carcinogenesis? – Commentary

2018 ◽  
Vol 1 (1) ◽  
pp. 20-23
Author(s):  
Daniel Benharroch
Keyword(s):  
Hodgkin Lymphoma ◽  
Measles Virus ◽  
Rt Pcr ◽  
Beer Sheva ◽  
Virus Rna ◽  
Classic Hodgkin Lymphoma ◽  
Repeated Calls ◽  
Measles Antibodies ◽  
International Experts

Objectives: An association between the measles virus and Hodgkin lymphoma has been disclosed by our laboratory in Beer-Sheva, starting in 2003. We question the refutation of our study and the absence of interest among experts. Methodology: It was based on immunohistochemistry with commercial, as well as experimental anti-measles antibodies. It relied also on RT-PCR and in situ hybridization evidence of measles virus RNA. Key Results: At this stage (2004), the link between the virus and the lymphoma was essentially descriptive. The first and last response to our challenge appeared in 2007, in the form of doublet articles, in the same issue of a major cancer journal. The two European research groups responding, rejected categorically our findings by proposing different arguments. Major Conclusion: As reservations to these reactions became soon apparent, a series of papers from our laboratory were published. These articles concerned the evidence of a relationship between the measles virus and additional categories of cancers. Different malignancies in which this virus was not expressed at all, were also described. A further study suggested a mechanism by which the measles virus may activate lymphomagenesis in classic Hodgkin lymphoma. To our dismay, and in spite of repeated calls to verify the various results, no further response was obtained from international experts.

HL-325: A Case Report of Classic Hodgkin Lymphoma with Unusually Abundant Tumor Cell Expression of B-Cell Markers: A Diagnostic Dilemma and Treatment Challenge

2021 ◽  
Vol 21 ◽  
pp. S372-S373
Author(s):  
Irina Panovska-Stavridis ◽  
Nevenka Ridova ◽  
Simona Stojanovska ◽  
Aleksandra Pivkova-Veljanovska ◽  
Sanja Trajkova ◽  
...  
Keyword(s):  
Case Report ◽  
Tumor Cell ◽  
B Cell ◽  
Hodgkin Lymphoma ◽  
Diagnostic Dilemma ◽  
Cell Markers ◽  
Classic Hodgkin Lymphoma ◽  
Cell Expression

Synergistic Effect of Quinacrine with Chemotherapeutics or TRAIL in Hematopoietic Malignant Cells

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5239-5239
Author(s):  
Wenge Wang ◽  
Amriti R. Lulla ◽  
Liz J. Hernandez-Borrero ◽  
David T. Dicker ◽  
Emmanuel K. Teye ◽  
...  
Keyword(s):  
Cell Death ◽  
Tyrosine Kinase ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Kinase Inhibitors ◽  
Cell Lymphoma ◽  
Chemotherapeutic Agents ◽  
Malignant Cells ◽  
Tumor Cell Death

Abstract Quinacrine is a bioactive acridine derivative which has been used for treatment of malaria, giardiasis, systemic lupus erythematosus, and rheumatoid arthritis. In searching for p53 pathway activating agents for cancer therapy, we found that quinacrine stabilizes p53 and induces p53-dependent and p53-independent tumor cell death. Quinacrine also induces expression of TRAIL Death Receptor 5 (DR5) and reduces expression of anti-apoptotic Mcl-1 in tumor cells. These activities predict synergies with TRAIL (tumor necrosis factor-related apoptosis inducing ligand) and chemotherapeutic agents in inducing extrinsic and intrinsic pathway mediated apoptosis. In addition, quinacrine suppresses NFkB activity in tumor cells. Clinical trials have been ongoing for treatment of solid tumors including colon cancer, renal cancer, prostate cancer, and non-small cell lung cancer with quinacrine in combination with chemotherapy or tyrosine-kinase inhibitors, however, the therapeutic potential of quinacrine in blood cancer cells has not been established. We tested quinacrine on hematopoietic malignant cells, which included cell lines of myeloid leukemia, lymphoblastic leukemia, B-cell lymphoma, T-cell lymphoma, and multiple myeloma. We found that quinacrine induces massive cell death in the cell lines tested, at concentrations from as less as 1 microM to 5 microM, 2-10 times lower than required to induce solid tumor cell death. Quinacrine synergizes with TRAIL in inducing cell death of TRAIL-sensitive cells and reverses resistance in TRAIL-resistant cells. Quinacrine also synergizes with chemotherapeutic agents, such as antimetabolites, alkylating agents, and tyrosine kinase inhibitors, in inducing apoptosis of hematopoietic cancer cell lines. Our work supports translational efforts to advance the use of quinacrine from bench to clinic and provides rationale for combination chemotherapeutic regimes for treatment of hematopoietic malignancies. Disclosures No relevant conflicts of interest to declare.

scholarly journals Flow Cytometric Detection of the Classical Hodgkin Lymphoma: Clinical and Research Applications

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Mikhail Roshal ◽  
Brent L. Wood ◽  
Jonathan R. Fromm
Keyword(s):  
B Cell ◽  
Hodgkin Lymphoma ◽  
Tumor Cells ◽  
B Cell Lymphoma ◽  
Clinical Samples ◽  
Malignant Cells ◽  
Classical Hodgkin Lymphoma ◽  
Flow Cytometric ◽  
Current State ◽  
Viable Tumor

Classical Hodgkin lymphoma (CHL) is a relatively uncommon B cell-derived neoplasm that presents with rare malignant cells in an abundant reactive background. The diagnosis of CHL currently relies on a combination of morphologic findings and immunohistochemical stains. With the exception of rare cases with dramatically increased malignant populations, isolation of pure viable tumor cells has not been historically possible. Recently, a reliable flow cytometric assay for direct detection and isolation of the malignant cells in this disease has been developed. This assay has proven useful diagnostically and has been clinically validated to have a very high sensitivity and nearly absolute specificity for the diagnosis of CHL in routine clinical samples. This paper describes the methodology for the flow cytometric detection of CHL in clinical samples as well as current state of evaluation of background lymphocytes as an adjunct diagnostic test. Also discussed are exciting research applications of the direct isolation of viable tumor cells in CHL. The current state of flow cytometric evaluation of nodular lymphocyte predominant Hodgkin lymphoma and T cell-rich large B cell lymphoma is also briefly discussed.

Poster: HL-325: A Case Report of Classic Hodgkin Lymphoma with Unusually Abundant Tumor Cell Expression of B-Cell Markers: A Diagnostic Dilemma and Treatment Challenge

2021 ◽  
Vol 21 ◽  
pp. S237
Author(s):  
Irina Panovska-Stavridis ◽  
Nevenka Ridova ◽  
Simona Stojanovska ◽  
Aleksandra Pivkova-Veljanovska ◽  
Sanja Trajkova ◽  
...  
Keyword(s):  
Case Report ◽  
Tumor Cell ◽  
B Cell ◽  
Hodgkin Lymphoma ◽  
Diagnostic Dilemma ◽  
Cell Markers ◽  
Classic Hodgkin Lymphoma ◽  
Cell Expression

scholarly journals The Immunosuppressive Microenvironment of Classic Hodgkin Lymphoma and Therapeutic Approaches to Counter It

2019 ◽  
Author(s):  
Donatella Aldinucci ◽  
Cinzia Borghese ◽  
Naike Casagrande
Keyword(s):  
T Cell ◽  
Tumor Microenvironment ◽  
Hodgkin Lymphoma ◽  
Tumor Cells ◽  
Current Knowledge ◽  
Treatment Strategies ◽  
T Cell Exhaustion ◽  
Normal Cells ◽  
Cell Exhaustion ◽  
Classic Hodgkin Lymphoma

Classic Hodgkin lymphoma (cHL) is characterized by a few tumor cells surrounded by a protective, immunosuppressive tumor microenvironment composed of normal cells that are an active part of the disease. Hodgkin and Reed-Sternberg (HRS) cells evade the immune system through a variety of different mechanisms. They are invisible to antitumor effector T cells and natural killer cells and promote T cell exhaustion. Using cytokines and extracellular vesicles, they recruit normal cells, induce their proliferation, and “educate” (i.e., reprogram) them to become immunosuppressive and protumorigenic. Therefore, alternative treatment strategies, targeting not only tumor cells but also the tumor microenvironment, are being developed. Here we summarize current knowledge on the ability of HRS cells to build their microenvironment and to educate normal cells to become protective or immunosuppressive. We also describe therapeutic strategies to counteract formation of the tumor microenvironment and related processes leading to T cell exhaustion and repolarization of immunosuppressive tumor-associated macrophages.

scholarly journals Cluster of Differentiation 274 Antigen Immunohistochemical Expression in Tumor and Peri-tumor Cells of Hodgkin and Non-Hodgkin Lymphoma and Clinicopathological Relation (Single-center Study)

2021 ◽  
Vol 9 (A) ◽  
pp. 1011-1018
Author(s):  
Walaa Ghanam ◽  
Shaimaa M. M. Bebars
Keyword(s):  
Hodgkin Lymphoma ◽  
Tumor Cells ◽  
Lymph Node Excision ◽  
Excision Biopsy ◽  
Immunohistochemical Expression ◽  
High Power Field ◽  
Non Hodgkin Lymphoma ◽  
Classic Hodgkin Lymphoma ◽  
Significant Expression ◽  
Cluster Of Differentiation

BACKGROUND: Cluster of differentiation 274 (CD274) antigen has been investigated in tumors to evaluate its regulation and effect as a predictive of targeted therapy. Its expression and effect in lymphoma have raised interest recently. However, results were mixed and showed wide variations. AIM: This study aims to explore and compare CD274 antigen immunohistochemical expression in tumor and peri-tumor cells of classic Hodgkin lymphoma (HL) and diffuse large B cells non-HL (NHL) and its relation with clinicopathological criteria. METHODS: This work was carried out on 78 cases of lymph node excision biopsy (48 HL and 30 NHL). Prepared sections were applied for immunohistochemistry using CD274 monoclonal rabbit anti-human (programmed cell death protein 1 [PD-L1] ZR3-ASR, a Sigma Aldrich company). Assessment of CD274 antigen in tumor cells was considered positive if detected in >10% (membranous staining with cytoplasmic accentuation). Peri-tumor cells were scored as: 0, no positive cells/high-power field (HPF); 1, <10 positive cells/HPF; 2, 10–30 positive cells/HPF; 3, >30 positive cells/HPF. RESULTS: CD274 antigen was expressed in 53.8% of total lymphoma cases with significantly more expression of CD274 antigen in HL than NHL (66.7% vs. 33.3%). Classic HL showed significantly higher expression of CD274 antigen in tumor and peri-tumor cells and significant association with elevated erythrocyte sedimentation rate and lactate dehydrogenase and male gender. INTERPRETATION AND CONCLUSION: There is a more frequent and significant expression of CD274 antigen in classic HL than NHL cases in tumor and peri-tumor cells and a significant association with bad prognostic criteria in classic HL. High expression of CD274 antigen in classic HL proposes its potential use as a marker, especially for prognostic indication.

Sign in / Sign up

Export Citation Format

Share Document