scholarly journals APPLICATION OF CAPILLARY ELECTROPHORESIS METHOD FOR DETERMINATION OF L-ARGININE CONTENT IN FEED ADDITIVE

2020 ◽  
Vol 21 (1) ◽  
pp. 153-161
Author(s):  
Н. P. Ryvak ◽  
T. R. Levytskyy ◽  
R. O. Ryvak ◽  
S. V. Chorniy
Keyword(s):  
Capillary Electrophoresis ◽  
Test Procedure ◽  
Amino Acid Content ◽  
Feed Additives ◽  
Feed Additive ◽  
Free Form ◽  
Limit Of Quantification ◽  
Test Technique ◽  
Definition Of

The article presents a literature review on the need to balance feed on L-arginine content, its characteristics, ways to supplement the diet of animals and poultry, as well as modern methods for quantitative determination of L-arginine in food, pharmaceuticals, etc. In the section «Materials and methods» the characteristic of the developed test technique, parameters of carrying out research, calibration characteristics with application of a standard sample of L-arginine and carrying out tests of amino acid content in a feed additive by means of system of capillary electrophoresis «Kapel-105M» are given. A description of the validation characteristics performed in the process of validation of the method is given. As a result of the conducted researches the technique of definition of the content of L-arginine in feed additives by means of a method of capillary electrophoresis is developed. The test procedure is based on the dissolution of the feed additive sample, further separation and quantification of the free form of L-arginine, its identification by individual absorption at a wavelength of 200 nm, temperature in the working capillary 30 ºC, voltage 25 kV and conductive electrolyte. The results of validation of this technique by the following characteristics are presented: repeatability, reproducibility, trueness, linearity, limit of quantification, budget uncertainty. The values of trueness, repeatability, reproducibility and uncertainty of the method (with a confidence level of (P) 0.95) does not exceed 5.0 %, the hypothesis of Linearity is acceptable, calculated on the basis of standard deviation (SD) limit of quantification for determining the content of arginine are satisfactory. The results allow us to conclude that the method of capillary electrophoresis using the device «Kapel-105M» is quite accurate and reliable in the case of studies of feed additives L-arginine with a content of the main substance of at least 98,0 %.

Application of the CEN/ISO Standard for Phytase Activity Measurements to a New Phytase Product: Determination of a Robust Conversion Factor by an Interlaboratory Study for Screening Feed Samples

2019 ◽  
Vol 102 (6) ◽  
pp. 1808-1813
Author(s):  
María José González de la Huebra ◽  
Piotr Robouch ◽  
Håkan Emteborg ◽  
Stefano Bellorini ◽  
Aneta Cizek-Stroh ◽  
...  
Keyword(s):  
Conversion Factor ◽  
Feed Additives ◽  
Phytase Activity ◽  
Feed Additive ◽  
The European Union ◽  
Iso Standard ◽  
Official Control ◽  
Activity Measurements ◽  
Feed Samples

Abstract Background: Phytase-based preparations are important feed additives currently authorised in the European Union (EU). The European Standard (EN) and International Organization for Standardization (ISO) standard 30024 describes a harmonized method for the determination of phytase activity and is fit-for-purpose for official control of a group of phytase products. However, it is not suitable for the determination of the phytase activity of a new feed additive encoded as 4a16 in the EU Register of Feed Additives, to which a slightly different phytase activity definition has been attributed. Objective: To establish a robust conversion factor to support official control laboratories that apply the EN ISO method when monitoring feed products containing 4a16. Methods: The phytase activity of test materials was determined by the participants using the EN ISO and/or the “applicant” methods. Results: Robust relative SDs for repeatability and for reproducibility of the methods applied for the determination of the phytase activity in the materials containing the 4a16 feed additive ranged from 2.6 to 22% (EN ISO method) and from 2.4 to 39% (applicant method). Conclusions: The data obtained confirmed the performance characteristics published for other phytase-based feeds in the related standard methods. These results allowed us to estimate a factor of 2.68 to convert phytase activities measured with the EN ISO method into the enzyme activity measured with the applicant method. Highlights: The obtained conversion factor will allow EU official laboratories to screen feed samples supplemented with the 4a16 phytase by applying EN ISO Standard 30024.

Application of the CEN/ISO Standard for Phytase Activity Measurements to a New Phytase Product: Determination of a Robust Conversion Factor by an Interlaboratory Study for Screening Feed Samples

2019 ◽  
Vol 102 (6) ◽  
pp. 1808-1813
Author(s):  
María José González de la Huebra ◽  
Piotr Robouch ◽  
Håkan Emteborg ◽  
Stefano Bellorini ◽  
Aneta Cizek-Stroh ◽  
...  
Keyword(s):  
Conversion Factor ◽  
Feed Additives ◽  
Phytase Activity ◽  
Feed Additive ◽  
The European Union ◽  
Iso Standard ◽  
Official Control ◽  
Activity Measurements ◽  
Feed Samples

Background: Phytase-based preparations are important feed additives currently authorised in the European Union (EU). The European Standard (EN) and International Organization for Standardization (ISO) standard 30024 describes a harmonized method for the determination of phytase activity and is fit-for-purpose for official control of a group of phytase products. However, it is not suitable for the determination of the phytase activity of a new feed additive encoded as 4a16 in the EU Register of Feed Additives, to which a slightly different phytase activity definition has been attributed. Objective: To establish a robust conversion factor to support official control laboratories that apply the EN ISO method when monitoring feed products containing 4a16. Methods: The phytase activity of test materials was determined by the participants using the EN ISO and/or the “applicant” methods. Results: Robust relative SDs for repeatability and for reproducibility of the methods applied for the determination of the phytase activity in the materials containing the 4a16 feed additive ranged from 2.6 to 22% (EN ISO method) and from 2.4 to 39% (applicant method). Conclusions: The data obtained confirmed the performance characteristics published for other phytase-based feeds in the related standard methods. These results allowed us to estimate a factor of 2.68 to convert phytase activities measured with the EN ISO method into the enzyme activity measured with the applicant method. Highlights: The obtained conversion factor will allow EU official laboratories to screen feed samples supplemented with the 4a16 phytase by applying EN ISO Standard 30024.

scholarly journals Influence of protein-mineral feed additive from marine aquatic organisms on growth intensity and nonspecific resistance of broiler chickens under different microclimate conditions

2020 ◽  
Vol 22 (99) ◽  
pp. 155-160
Author(s):  
N. I. Dankevych ◽  
V. Z. Salata
Keyword(s):  
Broiler Chickens ◽  
Bacterial Contamination ◽  
Specific Resistance ◽  
Aquatic Organisms ◽  
Feed Additives ◽  
Feed Additive ◽  
Growth Intensity ◽  
Microclimate Conditions ◽  
Broiler House

The conducted research was aimed at determination of the impact produced by feeding protein and mineral feed additive produced out of the primary processing aquatic organisms wastes: sea mussels, red algae as well as of the sea water upon the productivity and non-specific resistance of broiler chickens raised in conditions of the normative and non-normative characteristics of the broiler house microclimate. The feed additive was applied to 20–42 day broiler chickens of “Ross 308” cross. The studied broilers were clinically healthy. Throughout the entire experiment, a series of the sanitary and hygienic microclimate parameters were determined, such as temperature, humidity, rate of changes as well as bacterial contamination of air, content of ammonia and carbon dioxide in air, and illumination of the broiler house. The house temperature was measured every day with the aid of a common spirit-in glass thermometer. Air humidity was established with the aid of an August psychrometer, air draft speed, harmful gas concentrations and illumination indicators were measured in compliance with the generally accepted methods. Bacterial contamination was determined with the use of the method of microorganism precipitation on a solid breeding ground placed in Petri dishes followed by a count of the bacterial colonies per 1 m3. The blood analysis included determination of haemoglobin, erythrocytes and leukocytes. In the blood serum, the lysozyme activity (LABS) and bactericidal activity (BABS) were determined. It was established that enriching the basic ration with the protein and mineral additive in quantity of 7 % in addition to the feed mass under conditions of the normative microclimate produced a positive effect on the growth intensity, livability and non-specific resistance indices of the broiler chickens. Thus, the live weight of broilers was reliably greater by 4.7 % and the livability equalled 100 %. The haemoglobin content was reliably greater by 7.6 %, erythrocytes – by 11.5 %, BABS – by 34.5 % and LABS – by 35.9 % as compared with the control group of broiler chickens. At the same time, when the studied feed additive was fed to broiler chickens kept in the microclimate conditions that did not meet the normative requirements, the reliable difference to the control indices was not established. Hence, the research results have proved that application of the protein and mineral feed additive is effective under the optimal microclimate conditions. High figures of livability and growth intensity of broiler chickens are based on a high resistance which is being formed provided the optimal microclimate and application of feed additives have been provided.

scholarly journals Development and Validation of Sibutramine Determination in Drug Products by Capillary Electrophoresis

2020 ◽  
Vol 9 (4) ◽  
pp. 141-145
Author(s):  
A. M. Sukhanova ◽  
I. B. Perova ◽  
K. I. Eller ◽  
G. M. Rodioinova ◽  
S. V. Chernova ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Determination ◽  
Limit Of Detection ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Limit Of Quantification ◽  
Drug Products ◽  
Development And Validation

Introduction. Recently, there has been a growing trend in the number of obese and overweight patients. To date, sibutramine is the most effective drug for treating obesity and overweight. The drug is an inhibitor of the reuptake of serotonin and norepinephrine, which leads to a decrease in hunger, and therefore, to weight loss.Aim. To develop and validate a methodology for the determination of sibutramine in drugs by capillary electrophoresis (CE) using an ultraviolet diode array detector.Materials and methods. Quantitative determination of sibutramine in drugs was carried out using the CE method with an ultraviolet diode array detector. A solution of phosphate buffer 50 mmol pH = 7.0 was used as a solvent and working electrolyte; to separate the peaks – quartz capillary 56 cm, 50 μm.Results and discussion. The developed method was validated according to the following parameters: specificity, linearity, correctness, precision, limit of detection and limit of quantification.Conclusion. A method for the quantitative determination of sibutramine in drugs by the CE method using an ultraviolet diode array detector has been developed and validated. This method meets all the requirements of General Pharmacopoeia Monograph 1.1.0012.15 «Validation of the analytical method» and can be used to control the quality of drugs, the active pharmaceutical substance of which is sibutramine.

scholarly journals Titrimetric method determination of the content of the mass part of siliciumin feed additives

2020 ◽  
Vol 10 (1) ◽  
pp. 62-65
Author(s):  
O. V. Moravska ◽  
T. R. Levytskyy ◽  
S. O. Vovk
Keyword(s):  
Mass Fraction ◽  
Feed Additives ◽  
Hot Water ◽  
Feed Additive ◽  
Accuracy Control ◽  
Titrimetric Method ◽  
Mass Part ◽  
Powder Samples ◽  
Quadratic Deviation

The results of the development of the method for determining the mass fraction of silicium in powder samples of feed additives by the titrimetric method are presented in the article. The implementation and validation of the method was carried out with the use of feed additive of mixed type "Mikasil" produced by LLC «Globus», Ukraine. The procedure was reproduced ten times with the determination of the mass fraction of siliciumin two parallel samples. The method is based on the precipitation of silicic acid in the form of silicium-fluoride of potassium, followed by hydrolysis with hot water in the presence of chlorous calcium. The isolated hydro-chloric acid in an amount equivalent to the content of hydro-fluoric acid is titrating with alkali solution in the presence of an indicator. The results of the studies showed that the specified method for determining the mass fraction of silicium meets the standards of accuracy control for this method, namely the standard quadratic deviation (σ) is 0.52, when norm up to 0.7, when determined in two parallel samples. The results obtained indicate that the titrimetric method for determining the mass fraction of silicium in powder samples of feed additives is accurate, reliable, reproducible and economically available.

scholarly journals Chromatographic Determination of Amino Acids in Foods

2005 ◽  
Vol 88 (3) ◽  
pp. 877-887 ◽  
Author(s):  
Robert W Peace ◽  
G Sarwar Gilani
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Accurate Determination ◽  
Amino Acid Content ◽  
Food Sources ◽  
Free Form ◽  
Total Amino Acid ◽  
Protein Hydrolysis ◽  
Analytical Technique

Abstract Amino acids in foods exist in a free form or bound in peptides, proteins, or nonpeptide bonded polymers. Naturally occurring L-amino acids are required for protein synthesis and are precursors for essential molecules, such as co-enzymes and nucleic acids. Nonprotein amino acids may also occur in animal tissues as metabolic intermediates or have other important functions. The development of bacterially derived food proteins, genetically modified foods, and new methods of food processing; the production of amino acids for food fortification; and the introduction of new plant food sources have meant that protein amino acids and amino acid enantiomers in foods can have both nutritional and safety implications for humans. There is, therefore, a need for the rapid and accurate determination of amino acids in foods. Determination of the total amino acid content of foods requires protein hydrolysis by various means that must take into account variations in stability of individual amino acids and resistance of different peptide bonds to the hydrolysis procedures. Modern methods for separation and quantitation of free amino acids either before or after protein hydrolysis include ion exchange chromatography, high performance liquid chromatography (LC), gas chromatography, and capillary electrophoresis. Chemical derivatization of amino acids may be required to change them into forms amenable to separation by the various chromatographic methods or to create derivatives with properties, such as fluorescence, that improve their detection. Official methods for hydrolysis and analysis of amino acids in foods for nutritional purposes have been established. LC is currently the most widely used analytical technique, although there is a need for collaborative testing of methods available. Newer developments in chromatographic methodology and detector technology have reduced sample and reagent requirements and improved identification, resolution, and sensitivity of amino acid analyses of food samples.

scholarly journals Determination of Urinary Pterins by Capillary Electrophoresis Coupled with LED-Induced Fluorescence Detector

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1166 ◽  
Author(s):  
Wojciech Grochocki ◽  
Magdalena Buszewska-Forajta ◽  
Szymon Macioszek ◽  
Michał J. Markuszewski
Keyword(s):  
Capillary Electrophoresis ◽  
Limit Of Detection ◽  
Urine Samples ◽  
High Price ◽  
Range Limit ◽  
Uv Detector ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
Sample Stability

Urinary pterins have been found as potential biomarkers in many pathophysiological conditions including inflammation, viral infections, and cancer. However, pterins determination in biological samples is difficult due to their degradation under exposure to air, light, and heat. Besides, they occur at shallow concentration levels, and thus, standard UV detectors cannot be used without additional sample preconcentration. On the other hand, ultra-sensitive laser-induced fluorescence (LIF) detection can be used since pterins exhibit native fluorescence. The main factor that limits an everyday use of LIF detectors is its high price. Here, an alternative detector, i.e., light-emitted diode induced fluorescence (LEDIF) detector, was evaluated for the determination of pterins in urine samples after capillary electrophoresis (CE) separation. An optimized method was validated in terms of linearity range, limit of detection (LOD), limit of quantification (LOQ), intra- and interday precision and accuracy, sample stability in the autosampler, and sample stability during the freezing/thawing cycle. The obtained LOD (0.1 µM) and LOQ (0.3 µM) values were three-order of magnitude lower compared to UV detector, and two orders of magnitude higher compared to previously reported house-built LIF detector. The applicability of the validated method was demonstrated in the analysis of urine samples from healthy individuals and cancer patients.

The effects and mechanisms of acids on the health of piglets and weaners – a review

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Piotr Nowak ◽  
Anita Zaworska-Zakrzewska ◽  
Andrzej Frankiewicz ◽  
Małgorzata Kasprowicz-Potocka
Keyword(s):  
Digestive System ◽  
Buffer Capacity ◽  
Growth Parameters ◽  
Feed Additives ◽  
Feed Additive ◽  
Growth Promoters ◽  
Qualitative And Quantitative ◽  
Inorganic Acids ◽  
Low Activity ◽  
The Eu

AbstractThe rearing of piglets is the most difficult period in the pigs’ production because of their poorly developed digestive system and the low activity of digestive enzymes. Problems in nutrition and stress cause some disorders in the functioning of the digestive system leading to diarrhea and the mortality of piglets. Starting in 2006 in the EU, a total ban on antibiotics in their use as growth promoters was introduced. Since then, new and safe feed additives have been sought in order to replace antibiotics. Organic and inorganic acids as well as their salts were recognized as effective and safe additives. Due to their properties, they can improve feed palatability and digestibility, reduce the buffer capacity of feed, impact the development and functioning of the pig’s digestive system and improve the health and growth parameters. However, the effectiveness of acids is related to their qualitative and quantitative share in the feed additive. In this review, some strategies for using organic acids, their mixtures and also some new multi-component products will be discussed.

Spectrophotometeric Determination of Trifluoperazine HCL in Pure Forms and Pharmaceutical Preperations

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Mohammad Hamzah Hamzah ◽  
Rawa M M Taqi ◽  
Muna M. Hasan ◽  
Raid J. M. Al-Timimi
Keyword(s):  
Standard Method ◽  
Limit Of Detection ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Oxidizing Agent ◽  
Optimum Conditions ◽  
Limit Of Quantification ◽  
Cerium Ion ◽  
Blank Solution

A simple and accurate spectrophotometric method for the determination of Trifluoperazine HCl in pure and dosage forms was developed. The method is based on the reaction between Trifluoperazine HCl and p-chloroaniline in the presence of cerium ion as oxidizing agent which lead to the formation of violate color product that absorbed at a maximum wavelength 570nm while the blank solution was pink. Under the optimum conditions a linear relationship between the intensity and concentration of TRF in the range 4-50μg/ml was obtained . The molar absorptivity 3.74×103 L.mol-1.cm-1 , Limit of detection (2.21μg/ml), while limit of quantification was 7.39μg/ml. The proposed analytical method was compared with standard method using t-test and F-test , the obtained results shows there is no significant differences between proposed method and standard method. Based on that the proposed method can be used as an alternative method for the determination of TRF in pure and dosage forms.

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