The effect of Qatada (Acacia hamulosa) honey from Saudi Arabia on the steps of pilonidal sinus healing

The purpose of this study was to provide more light on the effect of Qatada (Acacia hamulosa) honey from Saudi Arabia as a dressing on pilonidal sinus wound healing, antibacterial activity, and cytokine levels. The results revealed that the pH of 3.56 of tested honey, where glucose was 378 mmol/l also the specific gravity was 1.435, while contents of total phenolic and flavonoid and DPPH activity (32.15 mg GAE/100 g honey), (54.27 mg RE/100 g honey) and (127.83 mg/ml) respectively. The mean duration of pilonidal sinus wounds to be ready for surgical closure was 43.5 days in the control (standard dressing) group where the honey dressing group was 28.5 days. Less pain, edema, and foul-smelling discharge were expressed in the honey group if compared with the standard dressing group. The total bacterial count was 9 × 109 at the begging of the experiment then it reduced after the application of standard dressing traditional therapy to (9 ×105) and honey treatment group (9 ×102) after 21 days post-treatment. Significant reduction (p<0.05) was observed in serum TNFα(-52), IL6 - 46), and IL-17 (-60) relatively to pre-treatment values. Based on our findings, we may conclude that Qatada honey bioactivities aided wound healing by increasing tissue growth while decreasing overall bacterial count and inflammation.

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Topical Saudi Arabia Talh honey (Acacia nilotica) on surgical wound healing activity

Highlights in BioScience ◽

10.36462/h.biosci.20220 ◽

2020 ◽

Author(s):

Ahmed G. Hegazi ◽

Faiz M. Al Guthami ◽

Mohamed H. Basiouny ◽

Ahmed F.M. Al Gethami

Keyword(s):

Wound Healing ◽

Saudi Arabia ◽

Wound Repair ◽

Healing Process ◽

Bacterial Count ◽

Tissue Growth ◽

Acacia Nilotica ◽

Total Bacterial Count ◽

Wound Healing Process ◽

Ifn Γ

Honey has been documented as the oldest traditional medicine. It has been effective in suppressing inflammation, wound repair enhancer, and rapid autolytic debridement. The aim of this investigation was to evaluate the role of Saudi Arabia Talh honey (Acacia nilotica) dressing as a good alternative in care of diabetic foot (DFU) healing activity for twenty patients, wound total bacterial count, and serum cytokines levels (IFN-γ, IL-1, and IL-6). The results showed that Talh honey stimulates the wound healing process, broad-spectrum antibacterial activity, and reduction in the proinflammatory cytokines IFN-γ, IL-1, and IL-6 levels. It could be concluded that Talh honey bioactivities enhance wound healing by promoting tissue growth leading to wound repair, antibacterial, and reduction of inflammation.

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Salmonella and Escherichia coli O157:H7 Inactivation, Color, and Bioactive Compounds Enhancement on Raspberries during Frozen Storage after Decontamination Using New Formula Sanitizer Washing or Pulsed Light

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-379 ◽

2016 ◽

Vol 79 (7) ◽

pp. 1107-1114 ◽

Cited By ~ 4

Author(s):

WENQING XU ◽

HAIQIANG CHEN ◽

CHANGQING WU

Keyword(s):

Frozen Storage ◽

Bacterial Count ◽

Quality Parameters ◽

Total Phenolic ◽

Total Bacterial Count ◽

Anthocyanin Content ◽

Escherichia Coli O157 ◽

Total Anthocyanin ◽

E Coli ◽

Total Anthocyanin Content

ABSTRACT Berries are normally washed before they are frozen. Washing with sanitizer and treatment with pulsed light (PL) were studied for their effectiveness to inactivate foodborne pathogens on raspberries during frozen storage, while maintaining or enhancing major quality parameters. Raspberries were inoculated with Salmonella or Escherichia coli O157:H7 and then underwent a washing treatment with citric acid plus sodium dodecyl sulfate (CA+SDS) or citric acid plus thymol (CA+THY) or treatment with PL (dry PL, water-assisted [wet] PL, and PL-SDS). Pathogen survival was determined immediately after treatments and during frozen storage at −20°C for 3 months. Washing with CA+SDS or CA+THY significantly reduced Salmonella (by 3.6 and 3.2 log CFU/g, respectively) and E. coli O157:H7 (by 4.1 and 3.7 log CFU/g, respectively). At the end of storage, washing with CA+SDS reduced Salmonella to 0.6 log CFU/g and E. coli O157:H7 to 0.5 log CFU/g; washing with CA+THY reduced Salmonella to 0.9 log CFU/g and E. coli O157:H7 to 0.5 log CFU/g. PL-SDS showed decontamination efficacy on raspberries, with 0.7 log CFU/g Salmonella and 0.9 log CFU/g E. coli O157:H7 surviving at the end of storage; in comparison, in the control, 1.6 log CFU/g Salmonella and 1.5 log CFU/g E. coli O157:H7 survived. Pathogen survival in raspberries that had been washed or treated with PL-SDS was significantly lower than in untreated raspberries. Major quality parameters, including color, total phenolic content, total anthocyanin content, total bacterial count, and total yeast and mold counts, were evaluated on raspberries immediately after treatments and during frozen storage. Redness increased in PL-treated raspberries. At the end of storage, PL-treated raspberries had significantly higher total phenolic content and total anthocyanin content compared with control samples. Washing with sanitizers and treatment with PL decreased the total bacterial count and total yeast and mold counts on raspberries and maintained the low counts. Our findings suggest that washing with a sanitizer or treatment with PL could be used to process frozen raspberries for enhanced food safety and quality.

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Improvement of Thanaka Powder by Gamma Radiation: Microbial and Chemical Properties

Chiang Mai University Journal of Natural Sciences ◽

10.12982/cmujns.2021.041 ◽

2021 ◽

Vol 20 (2) ◽

Author(s):

Wachiraporn Pewlong ◽

Surasak Sajjabut ◽

Sirilak Chookaew ◽

Jarurattana Eamsiri ◽

Khemruji Khemthong ◽

...

Keyword(s):

Gamma Radiation ◽

Gamma Irradiation ◽

Total Phenolic Content ◽

Phenolic Content ◽

Chemical Properties ◽

Bacterial Count ◽

Stem Bark ◽

Total Phenolic ◽

Total Bacterial Count ◽

Radiation Process

Thanaka powder is made from the stem bark of the Hesperethusa crenulata plant. Most Burmese women use it on the skin of the face as sun protection, a moisturizing agent and acne treatment. For a hygiene purpose, gamma radiation is currently used to control microorganisms and insect contamination in many products. In this experiment, the the effects of gamma radiation on the microbial numeration, total phenolic content, antioxidant activity, and chemical constituent alterations in Thanaka powder were investigated. Gamma irradiation was applied to powder samples at 5, 10, 15 and 20 kGy. The total bacterial count and total yeast and mold count were decreased with increasing irradiation dose. Gamma irradiation at a dose of 5 kGy was sufficient to reduce the microbial load in the powder to meet the standards of microbial quality for cosmetic products in Thailand. No significant change in the DPPH activity of the Thanaka powder was observed after irradiation. Additionally, the radiation process caused significant increases in total phenolic content, FRAP value and arbutin content. At a dose of 20 kGy, the total phenolic content, FRAP value and arbutin content were significantly higher than the non-irradiated Thanaka powder at 7.45, 9.59 and 16.04%, respectively.

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Pilonidal sinus disease: Karydakis flap procedure in our patients

Perspectives in Surgery ◽

10.33699/pis.2020.99.8.350-355 ◽

2020 ◽

Vol 99 (8) ◽

Keyword(s):

Wound Healing ◽

Pilonidal Sinus ◽

Healing Process ◽

Pilonidal Sinus Disease ◽

Wound Complications ◽

Limberg Flap ◽

New Disease ◽

Sinus Disease ◽

Flap Procedure

Introduction: Despite the available guidelines, opinions of many surgeons are quite ambiguous when it comes to the therapy of pilonidal sinus disease. The treatment can be a frustrating problem both for the surgeon and the patient because it is associated with wound complications and high recurrence rate. The objective of this study was to analyze the results of patients with pilonidal sinus disease undergoing the Karydakis flap procedure. Methods: A total of 27 patients treated for primary and recurrent pilonidal disease using the Karydakis flap procedure at our department between October 23, 2018 and November 22, 2019 were analyzed prospectively. We evaluated postoperative wound healing, complications and recurrence of the disease in a short-term follow-up period. Disease recurrence was defined as prolonged healing or as a new disease requiring repeated surgery. Results: In December 2019 all 27 patients came for a follow-up visit. The result was a fully lateralized wound without any signs of a new disease in all patients. In May 2020 a follow-up visit by phone was performed. The median follow-up was 12 months. The healing process was free of any serious complications in 25 patients. Seroma formation cases were managed by puncture in the outpatient setting. Conclusion: According to the available evidence and guidelines, off-midline procedures – the Karydakis flap, Bascom cleft lift, and Limberg flap procedures – are associated with lower recurrence rates and better wound healing. An important goal is to achieve complete wound lateralization and to change the configuration of the gluteal cleft by reshaping it, which results in a nicely flattened gluteal crease.

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The effect of seaweed extract on tomato plant growth, productivity and soil

Journal of Applied Phycology ◽

10.1007/s10811-021-02387-2 ◽

2021 ◽

Author(s):

Hashmath Inayath Hussain ◽

Naga Kasinadhuni ◽

Tony Arioli

Keyword(s):

Plant Growth ◽

Tomato Plant ◽

Root Zone ◽

Soil Health ◽

Dry Weight ◽

Bacterial Count ◽

Community Diversity ◽

Seaweed Extract ◽

Total Bacterial Count ◽

Soil Biology

AbstractThis study investigated the effects of seaweed extract (SWE) made from the brown algae Durvillaea potatorum and Ascophyllum nodosum on plants and soil. The application of SWE to soil growing tomato plants showed dual effects. SWE comprehensively improved tomato plant growth (flower clusters, flower number, fruit number, root length, root and shoot dry weight, SPAD) and increased plant productivity (yield and quality). Similarly, SWE application effected soil biology at the soil root zone by increasing total bacterial count and available soil nitrogen and impacting bacterial community diversity with an increase in certain bacterial families linked to soil health. A broader understanding of the effects of SWE on the plant-soil ecosystem may offer breakthrough approaches for sustainable food production.

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Selected Kefir Water from Malaysia Attenuates Hydrogen Peroxide-Induced Oxidative Stress by Upregulating Endogenous Antioxidant Levels in SH-SY5Y Neuroblastoma Cells

Antioxidants ◽

10.3390/antiox10060940 ◽

2021 ◽

Vol 10 (6) ◽

pp. 940

Author(s):

Muganti Rajah Kumar ◽

Swee Keong Yeap ◽

Han Chung Lee ◽

Nurul Elyani Mohamad ◽

Muhammad Nazirul Mubin Aziz ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Neuroblastoma Cells ◽

Annexin V ◽

Morphological Features ◽

Lower Percentage ◽

Total Phenolic ◽

Neuroprotective Effects ◽

Antioxidant Power ◽

Pre Treatment

Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six different kefir water samples were analysed by total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assays, whereas the anti-apoptotic activity on hydrogen peroxide (H2O2) induced SH-SY5Y cells was examined using MTT, AO/PI double staining, and PI/Annexin V-FITC assays. The surface and internal morphological features of SH-SY5Y cells were studied using scanning and transmission electron microscopy. The results indicate that Kefir B showed the higher TPC (1.96 ± 0.54 µg GAE/µL), TFC (1.09 ± 0.02 µg CAT eq/µL), FRAP (19.68 ± 0.11 mM FRAP eq/50 µL), and DPPH (0.45 ± 0.06 mg/mL) activities compared to the other kefir samples. The MTT and PI/Annexin V-FITC assays showed that Kefir B pre-treatment at 10 mg/mL for 48 h resulted in greater cytoprotection (97.04%), and a significantly lower percentage of necrotic cells (7.79%), respectively. The Kefir B pre-treatment also resulted in greater protection to cytoplasmic and cytoskeleton inclusion, along with the conservation of the surface morphological features and the overall integrity of SH-SY5Y cells. Our findings indicate that the anti-oxidative, anti-apoptosis, and neuroprotective effects of kefir were mediated via the upregulation of SOD and catalase, as well as the modulation of apoptotic genes (Tp73, Bax, and Bcl-2).

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Quality Assessment of Shrimp (Macrobrachium rosenbergii. De Man, 1879) During Storage in Ice

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v47i1.10731 ◽

2012 ◽

Vol 47 (1) ◽

pp. 93-98 ◽

Cited By ~ 2

Author(s):

M Begum ◽

MA Khaleque ◽

MA Wahed ◽

F Hafiz

Keyword(s):

Ph Value ◽

Macrobrachium Rosenbergii ◽

Bacterial Count ◽

Total Bacterial Count ◽

Time Interval ◽

Quality Changes ◽

Fish Sample ◽

Sensory Score ◽

De Man ◽

Score Evaluation

With a view to get a pen picture about the trend and extent of quality changes and as well as shelf life of the head off and head on shrimp (Macrobrachium rosenbergii, De Man, 1879), the present study have been carried out . Quality changes have been measured by application of sensory score evaluation and changes in chemical indices such as Total Volatile Nitrogen (TVN) value, pH and level of moisture at different time interval and Total Bacterial Count (TBC) at 15°C during the study period. The results of the sensory score evaluation indicates that the shrimp quality decreased to the limits of acceptability at 12th days of ice storage. The head off shrimp kept slight better than corresponding head on shrimps. The bacterial counts determined at 15°C have been found to increase from 103 - 107 and 104 -108 cfu/gm for head off and head on shrimp respectively at the limit of acceptability. The moisture level increased from initial 78.85gm% to final 83.80gm% (head off) & 79.0gm% to 84.0gm% (head on) and pH value increases from 7.1 to 8.1 during the whole period of iced preservation . Both head off and head on shrimp remained in acceptable condition for up to 12 and 14 days of storage respectively while the quality of fish deteriorates and become unacceptable. The bacterial count corresponding to unacceptable condition ranges from 108-109 cfu/gm fish sample. The TVN value corresponding to rejection condition were 45-50mg N/100gm fish sample. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.10731 Bangladesh J. Sci. Ind. Res. 47(1), 93-98, 2012

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Alkali pre-treatment of Sorghum Moench for biogas production

Chemical Papers ◽

10.2478/s11696-012-0298-0 ◽

2013 ◽

Vol 67 (9) ◽

Cited By ~ 12

Author(s):

Karina Michalska ◽

Stanisław Ledakowicz

Keyword(s):

Anaerobic Digestion ◽

Chemical Oxygen Demand ◽

Volatile Fatty Acids ◽

Biogas Production ◽

Oxygen Demand ◽

Total Phenolic ◽

Methane Generation ◽

Pre Treatment ◽

Alkali Hydrolysis ◽

Almost All

AbstractThis work studies the influence of the alkali pre-treatment of Sorghum Moench — a representative of energy crops used in biogas production. Solutions containing various concentrations of sodium hydroxide were used to achieve the highest degradation of lignocellulosic structures. The results obtained after chemical pre-treatment indicate that the use of NaOH leads to the removal of almost all lignin (over 99 % in the case of 5 mass % NaOH) from the biomass, which is a prerequisite for efficient anaerobic digestion. Several parameters, such as chemical oxygen demand, total organic carbon, total phenolic content, volatile fatty acids, and general nitrogen were determined in the hydrolysates thus obtained in order to define the most favourable conditions. The best results were obtained for the Sorghum treated with 5 mass % NaOH at 121°C for 30 min The hydrolysate thus achieved consisted of high total phenolic compounds concentration (ca. 4.7 g L−1) and chemical oxygen demand value (ca. 45 g L−1). Although single alkali hydrolysis causes total degradation of glucose, a combined chemical and enzymatic pre-treatment of Sorghum leads to the release of large amounts of this monosaccharide into the supernatant. This indicates that alkali pre-treatment does not lead to complete cellulose destruction. The high degradation of lignin structure in the first step of the pre-treatment rendered the remainder of the biomass available for enzymatic action. A comparison of the efficiency of biogas production from untreated Sorghum and Sorghum treated with the use of NaOH and enzymes shows that chemical hydrolysis improves the anaerobic digestion effectiveness and the combined pre-treatment could have great potential for methane generation.

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