scholarly journals Potential in Bioethanol Production from Various Agro Wastes Fermenting by Microorganisms Using Carrot Peel, Onion Peel, Potato Peel and Sugar Beet Peel as Substrates

2021 ◽  
Vol 3 (2) ◽  
pp. 27-31
Author(s):  
Isaie Mushimiyimana ◽  
Celestin Sirimu ◽  
Fidel Niyitanga ◽  
Leoncie Niyonsaba
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gas Chromatography ◽  
Sugar Beet ◽  
Fermentation Process ◽  
Raw Materials ◽  
Bioethanol Production ◽  
Distillation Process ◽  
Potato Peel ◽  
Onion Peel ◽  
Fermented Product

Large amount of agro wastes is produced in Rwanda each year. The global annual potential bioethanol production from the major vegetables wastes such as carrot peel, onion peel, potato peel and sugar beet peel were estimated. Those wastes processing was successfully used as raw materials for the production of bioethanol, employing by cellulase produced from various filamentous fungi including Cladosporium cladosporioides was used for hydrolysis and the fermentation of the hydrolyzed samples was done using Saccharomyces cerevisiae. The fermented product was purified by primary distillation process at 79°C and the fraction was collected. The ethanol is then determined by specific dichromate method and Gas Chromatography. Instantaneous saccharification and fermentation process yielded maximum ethanol in the substrate of carrot peel was 16.9% at 21st day and further confirmed by Gas chromatography and the yield of ethanol obtained was 15.8%.

scholarly journals POTENTIAL IN BIOETHANOL PRODUCTION FROM VARIOUS AGRO WASTES FERMENTING BY MICROORGANISMS USING CARROT PEEL, ONION PEEL, POTATO PEEL AND SUGAR BEET PEEL AS SUBSTRATES.

2021 ◽  
Vol 4 (2) ◽  
pp. 1-4
Author(s):  
Dr.isaie Mushimiyimana ◽  
Fidel Niyitanga ◽  
Celestin Sirimu
Keyword(s):  
Gas Chromatography ◽  
Sugar Beet ◽  
Fermentation Process ◽  
Raw Materials ◽  
Bioethanol Production ◽  
Distillation Process ◽  
Potato Peel ◽  
Onion Peel ◽  
Fermented Product ◽  
Sacharomyces Cerevisiae

Large amount of agro wastes are produced in Rwanda each year. The global annual potential bioethanol production from the major vegetables wastes such as carrot peel, onion peel, potato peel and sugar beet peel was estimated. Those wastes processing were successfully used as raw materials for the production of bioethanol, employing by cellulase produced from various filamentous fungi including Cladosporium cladosporioides was used for hydrolysis and the fermentation of the hydrolyzed samples was done using Sacharomyces cerevisiae. The fermented product was purified by primary distillation process at 79 °C and the fraction was collected. The ethanol is then determined by specific dichromate method and Gas Chromatography. Instantaneous saccharification and fermentation process yielded maximum ethanol in the substrate of carrot peel was 16.9 % at 21st day and further confirmed by Gas chromatography and the yield of ethanol obtained was 15.8 %.

scholarly journals Selenium supplementation during fermentation with sugar beet molasses and Saccharomyces cerevisiae to increase bioethanol production

2019 ◽  
Vol 8 (1) ◽  
pp. 622-628 ◽  
Author(s):  
Sara Faramarzi ◽  
Younes Anzabi ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sugar Beet ◽  
Sodium Selenite ◽  
Fermentation Process ◽  
Selenium Supplementation ◽  
Bioethanol Production ◽  
Beet Molasses ◽  
Sugar Beet Molasses ◽  
Fermentation Parameters ◽  
Microbial Strain

Abstract A bench scale submerged fermentation process was used to bioethanol produce using sugar beet molasses and Saccharomyces cerevisiae, as substrate and microbial strain, respectively. Effects of selenium amount on growth of S. cerevisiae and bioethanol production were evaluated. The obtained results indicated that growth of S. cerevisiae (manifested as turbidity intensity) in the samples containing 0, 5, 10, 15, 20 and 25 μg sodium selenite, during aerobic process, was 0.1707, 0.1678, 0.1679, 0.1664, 0.1627 and 0.160% a.u./h (after 14 h incubation), respectively. Statistical analysis based on compression test indicated that there were insignificant (p > 0.05) differences between growth rate of the yeast in the fermented samples containing S. cerevisiae and 5 to 25 μg selenium salt. Response surface methodology was utilized to evaluate effects of two fermentation parameters namely, amount of selenium (5-25 μg) and substrate brix (10-25°Bx) on the concentration (g/L) of produced bioethanol. Obtained results revealed that maximum bioethanol concentration (55 g/L) was achieved using 15 μg selenium and molasses with 25°Bx. Furthermore, results have also indicated that, without using selenium and using molasses with 25°Bx, bioethanol with concentration of 29 g/L was produced.

Renewable Energy from Agricultural Waste

2018 ◽  
Vol 69 (6) ◽  
pp. 1363-1366 ◽  
Author(s):  
Stefania Daniela Bran ◽  
Petre Chipurici ◽  
Mariana Bran ◽  
Alexandru Vlaicu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gas Chromatography ◽  
Continuous Flow ◽  
Laboratory Experiments ◽  
Fermentation Process ◽  
Flow Reactor ◽  
Agricultural Waste ◽  
Continuous Flow Reactor ◽  
Natural Support ◽  
Co2 Flow

This paper has aimed at evaluating the concentration of bioethanol obtained using sunflower stem as natural support, molasses as carbon source and Saccharomyces cerevisiae yeast in a continuous flow reactor. The natural support was tested to investigate the immobilization/growth of S. cerevisiae yeast. The concentration of bioethanol produced by fermentation was analyzed by gas chromatography using two methods: aqueous solutions and extraction in organic phase. The CO2 flow obtained during the fermentation process was considered to estimate when the yeast was deactivated. The laboratory experiments have highlighted that the use of plant-based wastes to bioconversion in ethanol could be a non-pollutant and sustainable alternative.

scholarly journals Influence of organic acids and organochlorinated insecticides on metabolism of Saccharomyces cerevisiae

2005 ◽  
pp. 207-215 ◽  
Author(s):  
Dusanka Pejin ◽  
Vesna Vasic
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Sugar Beet ◽  
Specific Growth Rate ◽  
Glycogen Content ◽  
Specific Growth ◽  
Raw Materials ◽  
Stress Factors ◽  
Adaptive Mechanism ◽  
Ribonucleic Acids

Saccharomyces cerevisiae is exposed to different stress factors during the production: osmotic, temperature, oxidative. The response to these stresses is the adaptive mechanism of cells. The raw materials Saccharomyces cerevisiae is produced from, contain metabolism products of present microorganisms and protective agents used during the growth of sugar beet for example the influence of acetic and butyric acid and organochlorinated insecticides, lindan and heptachlor, on the metabolism of Saccharomyces cerevisiae was investigated and presented in this work. The mentioned compounds affect negatively the specific growth rate, yield, content of proteins, phosphorus, total ribonucleic acids. These compounds influence the increase of trechalose and glycogen content in the Saccharomyces cerevisiae cells.

scholarly journals PENGARUH KADAR Aspergillus niger TERHADAP PRODUKSI BIOETANOL DARI BONGGOL PISANG KEPOK ( Musa paradisiaca L)

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Junaini Junaini ◽  
Elvinawati Elvinawati ◽  
Sumpono Sumpono
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Specific Gravity ◽  
Fermentation Process ◽  
Bioethanol Production ◽  
Gravity Method ◽  
Musa Paradisiaca ◽  
Ethanol Content

This study aims to determine the effect of Aspergillus niger levels on bioethanol production in banana cobs using Saccharfication Simultation Fermentation (SSF) method. This research uses banana kepok (Musa paradisiaca L.) obtained from Enggano Island of Bengkulu Province. Enggano Island is one of the outermost islands of Bengkulu Province which has a coordinate point of 5023'25,000 '' LS - 102014'16,000 '' BT. Samples of banana done preparation before the hydrolysis and fermentation process by smoothing the banana cobs using a blender until it becomes mush. Samples in the form of slurry were then added by Aspergillus niger and Sccharomyces cerevisiae. Hydrolysis performed for 72 hours which then continued with the fermentation process for 5 days. In the study there were 5 treatments: addition of Aspergillus niger 107 CFU/mL, addition of 10 mL Saccharomyces cerevisiae, addition of 10 mL Saccharomyces cerevisiae + Aspergillus niger 106 CFU/mL, 10 mL Saccharomyces cerevisiae + Aspergillus niger 107CFU/mL and 10 mL Saccharomyces cerevisiae + Aspergillus niger 108CFU/mL. The fermentation results were distilled and then measured the ethanol content by the specific gravity method. Ethanol content obtained from each treatment were 3.995%, 6.218%, 6.825%, 9.065%, and 12.348%, respectively. From one-way analysis test can be obtained the value of Fcount and Ftabel respectively are 25.73 and 5.19, so the value of Ftable< Fcount which means each treatment has a different result significantly.

Production of Bio-Ethanol via Hydrolysis and Fermentation Using Cassava Peel and Used Newspaper as Raw Materials

2020 ◽  
Vol 981 ◽  
pp. 222-227
Author(s):  
Tintin Mutiara ◽  
Siska Widiawati ◽  
Syafira Rachmatyah ◽  
Achmad Chafidz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Energy Source ◽  
Renewable Energy Source ◽  
Gas Chromatography ◽  
Manihot Esculenta ◽  
Raw Materials ◽  
Solid Wastes ◽  
Waste Materials ◽  
Fermentation Time ◽  
Cassava Peel

Cassava peel and used newspaper are example of daily solid wastes that continue to increase in Indonesia from year to year. Most of people in Indonesia have not utilized cassava (Manihot Esculenta L.) peel and used newspaper properly, hence they only become untreated wastes. Cassava peel highly contains carbohydrate and starch which is about 44 - 59%, while newsprint contains quite a lot of cellulose which is 61%, so that both wastes have the potential to make bio-ethanol as a one of potential renewable energy source. The focus of this study is to produce bio-ethanol from these two waste materials. There were several stages to produce bio-ethanol, which are hydrolysis with 0.1 N hydrochloric acid (HCl), and fermentation with yeast (Saccharomyces Cerevisiae), and separation of the bio-ethanol by distillation. The content of bio-ethanol produced was analyzed by Gas Chromatography (GC). The bio-ethanol was produced under different variables, which are: amount of yeast (8 g and 10 g), fermentation time (3, 7, and 10 days), and ratio of volume of solution (mL) cassava peel waste : used newspaper (i.e. 80:20; 60:40; 50:50; 40:60). The GC analysis results showed that the bio-ethanol contents were approximately 0%, 6.194%, 8.421% and 8.887% for ratio volume of solution (mL) cassava peel waste : used newspaper of 80:20; 60:40; 50:50; 40:60, respectively. Whereas, the bio-ethanol contents for 3, 7, and 10 days of fermentation time were 6.690%, 8.765%, and 9.472%, respectively. In this study, the highest bio-ethanol content produced (i.e. 9.472%) was in the following variables: amount of yeast 10 g, 10 days fermentation time, ratio volume of solution (mL) cassava peel waste : used newspaper of 50:50.

scholarly journals PEMBUATAN BIOETANOL BERBAHAN BAKU AMPAS TEBU DAN KULIT PISANG DENGAN VARIASI MASSA RAGI

JTAM ROTARY ◽  
2020 ◽  
Vol 2 (1) ◽  
pp. 123
Author(s):  
Fahron Anwar ◽  
Rachmat Subagyo
Keyword(s):  
Gas Chromatography ◽  
Fermentation Process ◽  
Ethanol Yield ◽  
Banana Peel ◽  
Distilled Water ◽  
Distillation Process ◽  
Fermentation Time ◽  
Ethanol Content

Penelitian ini bertujuan untuk menentukan kadar bioetanol terbaik dari kombinasi campuran ampas tebu dan pisang dengan variasi massa ragi 4 gram, 6 gram dan 8 gram dengan waktu fermentasi 60 jam, untuk menentukan massa ragi yang optimal dan untuk menentukan kadar etanol sesuai untuk SNI. Penelitian ini dilakukan dengan cara hidrolisis atau pendidihan ampas tebu dan kulit pisang untuk memecah molekul menjadi dua bagian dengan air suling, kemudian proses fermentasi dilakukan menggunakan Saccharomyces Cerevisae (ragi) sesuai variasi yang ditentukan dan proses penyulingan dilakukan menggunakan destilator untuk mendapatkan etanol dari fermentasi kemudian diuji dengan Refractometer Pen untuk mengetahui apakah ada etanol yang terbentuk dari proses distilasi. Sampel terbaik yang dipilih kemudian diuji kandungan etanol menggunakan alat Gas Chromatography. Jadi hasil etanol terbaik yang dapat dikategorikan sebagai pencapaian dalam SNI adalah kombinasi dari 75% ampas tebu - 25% kulit pisang dengan penambahan 8 gram ragi dan etanol yang diproduksi sebesar 96,64%. This study aims to determine the best bioethanol levels from a combination of bagasse and banana peel mixtures with variations in yeast mass of 4 grams, 6 grams and 8 grams with a fermentation time of 60 hours, to determine the optimum yeast mass and to determine ethanol levels according to SNI. This research was carried out by hydrolysis or boiling of bagasse and banana peel to break down molecules into two parts with distilled water, then the fermentation process was carried out using Saccharomyces Cerevisae (yeast) according to the specified variation and the distillation process was carried out using a destilator to get ethanol from fermentation then tested with a Refractometer Pen to find out if there is ethanol formed from the distillation process. The best sample selected then tested the ethanol content using the Gas Chromatography tool. So the best ethanol yield that can be categorized as achievement in SNI is a combination of 75% bagasse - 25% banana peel with the addition of 8 grams of yeast and ethanol produced at 96.64%.

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