Effect of Short-term Heat-induced Stress on Complete Blood Counts (CBCs) in the Swiss Albino Mice (Mus musculus)

Sixty sexually mature, 15- to 22-week-old, male and female laboratory-bred Swiss albinos weighing 21 to 43g each were used in the present study to find out the effect of exposing mice to 35 to 40C0 for 4 hours on their blood parameters using Complete Blood Counts (CBC) as indicators. The experimental design was 4 groups of 2 X 2 factorial arrangements for genders and heat treatments. The genders were: 30 male mice and 30 female mice per treatment. The heat treatments were: Control: 30 mice (15 males and 15 females) kept for four hours at room temperature (25 ± 2 C0), and the heat stress: 30 mice (15 males and 15 females) kept at 35 to 40 0C ambient temperature for four hours. The variables measured were: white blood corpuscles (WBC), red blood corpuscles (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and platelets count (PLT). Exposure to the heat produced a profound effect on the blood. Differences in levels of WBC, MCHC, and PLT in males and females heat exposed groups were not significant, but those of the rest (RBC, HGB, HCT, MCV, and MCH) were significant. Keywords: Albino mice, Mus musculus, heat stress, Complete Blood Count

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CLINICOPATHOLOGICAL EFFECTS OF HEAT STRESS ON BLOOD PICTURE OF JAPANESE QUAILS

International Journal of Advanced Research ◽

10.21474/ijar01/12923 ◽

2021 ◽

Vol 9 (5) ◽

pp. 913-916

Author(s):

Khurshaid Anwar ◽

◽

Nakash Smark ◽

Sartaj Khan ◽

Kalim Ullah ◽

...

Keyword(s):

Heat Stress ◽

Blood Cells ◽

White Blood Cells ◽

Hemoglobin Concentration ◽

Coturnix Japonica ◽

Mean Corpuscular Hemoglobin ◽

Coturnix Coturnix Japonica ◽

Corpuscular Hemoglobin ◽

Coturnix Coturnix ◽

Japanese Quails

This study aimed to evaluate the effects of heat stress on quail hematological parameters two published papers on quails in 2013 were studied, analyzed and compared. Broiler Japanese quails (Coturnix coturnix japonica) were exposed to high ambient temperature (340C, 8hr/d, 0900-1700hr) in both papers. An increase in values of red blood cells count, white blood cells count, lymphocytes, heterophils and lymphocytes (H/L) ratio, Monocytes, packed cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration were recorded while a lower values of heterophils, basophils, eosinophils, mean corpuscular volume and hemoglobin concentration than normal were recorded in groups exposed to heat stress (340C) on day 42 in Japanese quails (Coturnix coturnix japonica). Hemoglobin concentration was least affected. The obtained results indicated that, rearing quail birds under high environmental conditions during the fattening period has a great adverse effect on blood parameters. To help maintain Japanese quail as an essential animal model further heat stress related research on modern ways is needed.

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Narrowed reference intervals for complete blood count in a multiethnic population

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2018-1263 ◽

2019 ◽

Vol 57 (9) ◽

pp. 1382-1387

Author(s):

Seungok Lee ◽

Chui Mei Ong ◽

Yu Zhang ◽

Alan H.B. Wu

Keyword(s):

San Francisco ◽

Blood Count ◽

Complete Blood Count ◽

Hemoglobin Concentration ◽

Reference Intervals ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Multiethnic Population ◽

Race Ethnicity ◽

Eosinophil Counts

Abstract Background Biological variation studies have shown that the complete blood count (CBC) has narrow within-individual variation and wide group variation, indicating that the use of reference intervals (RIs) is challenging. The aim of this study was to examine differences in CBC RIs according to race/ethnicity in a multiethnic population at a hospital in San Francisco in hopes of improving the medical utility of CBC testing. Methods Subject data were obtained by screening CBC results from the medical records of outpatients meeting certain criteria who visited Zuckerberg San Francisco General Hospital from April 2017 to January 2018. From these records, sex- and race/ethnicity-specific CBC RIs were calculated as the 2.5th to 97.5th percentiles. Results From a total of 552 subjects, 47.9% were male (65 White, 50 Black, 71 Hispanic and 54 Asian) and 52.1% were female (51 White, 39 Black, 122 Hispanic and 72 Asian). The RIs of neutrophil, lymphocyte and eosinophil counts; and hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) showed significant differences (p<0.05) among the four racial/ethnic groups: neutrophil, lymphocyte and eosinophil counts; and MCHC in males, and hemoglobin, MCV, MCH and MCHC in females. Conclusions Race/ethnicity-specific CBC RIs should be taken into consideration in a multiethnic population to better interpret patient status and make progress toward precision medicine.

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Effect of Resistance Training on Hematological Blood Markers in Older Men and Women: A Pilot Study

Current Gerontology and Geriatrics Research ◽

10.1155/2009/156820 ◽

2009 ◽

Vol 2009 ◽

pp. 1-4 ◽

Cited By ~ 12

Author(s):

Florian Bobeuf ◽

Mélissa Labonté ◽

Abdelouahed Khalil ◽

Isabelle J. Dionne

Keyword(s):

Resistance Training ◽

Large Population ◽

Hemoglobin Concentration ◽

Blood Parameters ◽

Mean Corpuscular Hemoglobin ◽

Older Individuals ◽

Corpuscular Hemoglobin ◽

Distribution Width ◽

Men And Women ◽

Significant Difference

The aim of this study was to examine the effects of resistance training on hematological blood markers in older individuals. Twenty-nine men and women participated to this study. Subjects were randomized in 2 groups: (1) control (n=13) and (2) resistance training (n=16). At baseline and after the intervention, subjects were submitted to a blood sample to determine their hematological profile (red blood cells, hemoglobin, hematocrit, platelets, leukocytes, neutrophils, lymphocytes, monocytes, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width). At baseline, no difference was observed between groups. Moreover, we found no significant difference after the intervention on any of these markers. A 6-month resistance program in healthy older individuals seems to have no beneficial nor deleterious effects on hematological blood parameters. However, resistance training was well tolerated and should be recommended for other health purposes. Further studies are needed to confirm these results in a large population.

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Hematological parameters of Hoplias malabaricus (Characiformes: Erythrinidae) parasitized by Monogenea in lagoons in Pirassununga, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612013000400003 ◽

2013 ◽

Vol 22 (4) ◽

pp. 457-462 ◽

Cited By ~ 4

Author(s):

Lincoln Lima Corrêa ◽

Letícia Cucolo Karling ◽

Ricardo Massato Takemoto ◽

Paulo Sérgio Ceccarelli ◽

Marlene Tiduko Ueta

Keyword(s):

Condition Factor ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Good Health ◽

Blood Parameters ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Hoplias Malabaricus ◽

Relative Condition Factor ◽

The Mean

Hematology is an important pathological and diagnostic tool. This paper describes the hematological parameters of 76 specimens of Hoplias malabaricus infested with monogenean parasites, which were collected from two lagoons. The hematological parameters included: erythrocyte count (Er), microhematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood parameters showed no significant changes associated with the infestation. Water temperature was found to be negatively correlated with MCV and Hct, with values of rs= −0.52, p<0.0001 and rs= −0.48, p<0.0001, respectively. The mean Relative Condition Factor was Kn=1.01, indicating good health conditions of the fish in these lagoons. No correlation was found between the monogenean infestation and the blood parameters, or between the environmental factors and the monogeneans.

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365 The Extent and Pattern of Correlation Among Blood Parameters Differed Between Pasture-raised Goats and Sheep

Journal of Animal Science ◽

10.1093/jas/skab235.367 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 202-203

Author(s):

Uma Karki ◽

Anand Tiwari ◽

Kendra Norwood ◽

Ja’Nia Johnson ◽

Lila B Karki

Keyword(s):

Pearson Correlation ◽

Correlation Coefficients ◽

Hemoglobin Concentration ◽

Nutrient Status ◽

Blood Parameters ◽

Mean Corpuscular Hemoglobin ◽

Gamma Glutamyl Transferase ◽

Corpuscular Hemoglobin ◽

Correlation Pattern ◽

Glutamyl Transferase

Abstract Various blood parameters are generally used to monitor the nutrient status and health condition of goats and sheep. Relationship of a parameter with others may be useful to depict a bigger picture when that parameter is known. However, information on how different blood parameters are related to each other in goats and sheep has not been documented well. The objective of this study was to determine the correlation among different blood parameters in goats and sheep, both within and between species. Kiko does (19; 15–16 m old; live wt. 34±1.4 kg) and Katahdin ewes (18; 21–22 m old; live wt. 40±1.4 kg) were rotationally stocked in fall pastures for 87 days. Blood samples were collected on Day 1, Day 47, and Day 87, and analyzed for 34 blood parameters. Data were analyzed for Pearson Correlation Coefficients (r) (ɑ= 0.05) in SAS 9.4. In does, concentration of red blood cells (RBC) was found positively correlated with hemoglobin (HGB) (r=0.68), mean corpuscular hemoglobin concentration (MCHC) (r=0.36), albumin (r=0.36), and cholesterol concentrations (r=0.34) (P < 0.05). However, RBC concentration was negatively correlated with mean corpuscular volume (MCV) (r=-0.58) and mean corpuscular hemoglobin (MCH) (r=-0.71) (P < 0.0001). Cholesterol was found positively correlated with hematocrit, hemoglobin, lymphocyte, basophil, calcium, albumin, and chlorine, and negatively correlated with neutrophil and amylase (P < 0.05). In ewes, RBC concentration was found positively correlated with hematocrit (r=0.81) and hemoglobin (r=0.84) (P < 0.0001), and negatively correlated with MCV (r=-0.29), MCH (r=-0.59), MCHC (r=-0.42) and mean platelet volume (r=-0.39) (P < 0.05). Cholesterol was found positively correlated with creatinine, calcium, gamma-glutamyl transferase, and potassium, and negatively correlated with glucose, blood urea nitrogen, and lipase (P < 0.05). Results show that blood parameters are correlated with one another, and the correlation pattern and extent among blood parameters occur differently in goats and sheep.

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Complete Blood Count Peculiarities in Pregnant SARS-CoV-2-Infected Patients at Term: A Cohort Study

Diagnostics ◽

10.3390/diagnostics12010080 ◽

2021 ◽

Vol 12 (1) ◽

pp. 80

Author(s):

Roxana Covali ◽

Demetra Socolov ◽

Razvan Socolov ◽

Ioana Pavaleanu ◽

Alexandru Carauleanu ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Blood Count ◽

Complete Blood Count ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Mean Corpuscular Hemoglobin Concentration ◽

Blood Cell Count ◽

Corpuscular Hemoglobin ◽

Distribution Width

Background: During viral outbreaks, pregnancy poses an increased risk of infection for women. Methods: In a prospective study, all patients admitted for delivery at term to Elena Doamna Obstetrics and Gynecology University Hospital in Iasi, Romania, between 1 April 2020 and 31 December 2020 were included. There were 457 patients, divided into two groups: group 1, SARS-CoV-2-positive patients (n = 46) and group 2, SARS-CoV-2-negative patients (n = 411). Among other tests, complete blood count was determined upon admittance, and the following values were studied: white blood cell count, lymphocytes, neutrophils, red blood cell count, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, red blood cell distribution width, hematocrit, platelet count, mean platelet volume, platelet distribution width, plateletcrit, and platelet large cell ratio. Results: in pregnant SARS-CoV-2-infected patients at term, there was a significant decrease in white blood cell, neutrophil, and lymphocyte count, and an increase in mean corpuscular hemoglobin concentration, compared to healthy pregnant women at term, although all still within normal limits. None of the other components of the complete blood count or fetal outcomes studied was significantly influenced by SARS-CoV-2 infection in pregnant patients at term.

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725. Complete Blood Count Values Vary in Degree of Change with Day of Fever in Children with Dengue Fever

Open Forum Infectious Diseases ◽

10.1093/ofid/ofab466.922 ◽

2021 ◽

Vol 8 (Supplement_1) ◽

pp. S461-S462

Author(s):

Melissa E Day ◽

Miguel E Mejia Sang ◽

Yonairy Collado Puello ◽

Elvira J Diaz Brockmans ◽

Stephanie Rivera Defillo ◽

...

Keyword(s):

Dominican Republic ◽

Blood Cell ◽

Mean Corpuscular Volume ◽

Blood Count ◽

Complete Blood Count ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Volume ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin

Abstract Background Dengue fever (DF) is an acute viral disease which can lead to severe illness, including dengue hemorrhagic fever, marked by thrombocytopenia and hemolytic anemia, as well as end-organ damage. Despite the well-known presentation and prevalence, changes in hematologic markers across the DF course have not been well-described in children. We sought to investigate the association of clinical laboratory values over time with dengue disease progression and outcome in a pediatric population in the Dominican Republic. Methods Pediatric participants were enrolled at Hospital Infantil Dr. Robert Reid Cabral in Santo Domingo, Dominican Republic, in a prospective, observational case-based study. Laboratory values, including complete blood count (CBC) indices and dengue titer results, were collected over the course of hospital stay. Using linear mixed models, we assessed whether 13 different CBC values and time trajectories differed by dengue status, including age and sex as covariates. To account for multiple testing, p≤0.0033 was considered significant. Results A total of 575 children ages 0 to 211 months met inclusion criteria; 51.8% (n=298) were male, and the median (IQR) age was 59 (14-93) months. Eighty-two percent (n=472) of participants had DF. CBC values across days 1 to 10 of fever in those with and without DF are depicted in Figure 1. Those with DF showed levels dropping more quickly across days of fever for hematocrit and hemoglobin (p≤ 0.002), with a more rapid decline in those with severe DF (p < 0.0001). Those with DF had levels increasing more quickly for mean corpuscular hemoglobin concentration (MCHC), monocyte number, and white blood cell counts (p ≤ 0.003), with those with severe DF having a more rapid increase (p < 0.001). The direction of the change across time differed by DF status for mean corpuscular volume and red blood cell distribution width (RDW) (p ≤ 0.0003), with those with severe DF showing an increase in RDW across day of fever (p= 0.0004). Figure 1. CBC values across day of fever in dengue (blue) and non-dengue (purple) patients. The graph above depicts the following CBC values across day of fever in dengue (blue) and non-dengue (purple) patients: a) white blood cell (WBC) count, b) platelet count, c) monocyte number, d) hemoglobin, e) mean corpuscular hemoglobin concentration (MCHC), and f) mean corpuscular volume (MCV). Values with an asterisk (*) represent significant values (p < 0.0033). Conclusion The trajectory of CBC measures differs between those with and without DF, despite similar clinical presentations. These laboratory differences may facilitate a better understanding of the clinical course of DF and may aid in earlier identification of DF in resource-limited settings. Disclosures Elizabeth P. Schlaudecker, MD, MPH, Pfizer (Grant/Research Support)Sanofi Pasteur (Advisor or Review Panel member)

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A novel score system of blood tests for differentiating Kawasaki disease from febrile children

PLoS ONE ◽

10.1371/journal.pone.0244721 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0244721

Author(s):

Chih-Min Tsai ◽

Chi-Hsiang Chu ◽

Xi Liu ◽

Ken-Pen Weng ◽

Shih-Feng Liu ◽

...

Keyword(s):

Kawasaki Disease ◽

Alanine Aminotransferase ◽

Complete Blood Count ◽

Characteristic Curve ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

C Reactive Protein ◽

Corpuscular Hemoglobin ◽

Score System ◽

Reactive Protein

Background Kawasaki disease is the most common cause of acquired heart disease among febrile children under the age of 5 years old. It is also a clinically diagnosed disease. In this study, we developed and assessed a novel score system using objective parameters to differentiate Kawasaki disease from febrile children. Methods We analyzed 6,310 febrile children and 485 Kawasaki disease subjects in this study. We collected biological parameters of a routine blood test, including complete blood count with differential, C-reactive protein, aspartate aminotransferase, and alanine aminotransferase. Receiver operating characteristic curve, logistic regression, and Youden’s index were all used to develop the prediction model. Two other independent cohorts from different hospitals were used for verification. Results We obtained eight independent predictors (platelets, eosinophil, alanine aminotransferase, C-reactive protein, hemoglobin, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and monocyte) and found the top three scores to be eosinophil >1.5% (score: 7), alanine aminotransferase >30 U/L (score: 6), and C-reactive protein>25 mg/L (score: 6). A score of 14 represents the best sensitivity value plus specificity prediction rate for Kawasaki disease. The sensitivity, specificity, and accuracy for our cohort were 0.824, 0.839, and 0.838, respectively. The verification test of two independent cohorts of Kawasaki disease patients (N = 103 and 170) from two different institutes had a sensitivity of 0.780 (213/273). Conclusion Our findings demonstrate a novel score system with good discriminatory ability for differentiating between children with Kawasaki disease and other febrile children, as well as highlight the importance of eosinophil in Kawasaki disease. Using this novel score system can help first-line physicians diagnose and then treat Kawasaki disease early.

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Preliminary evaluation of complete blood count and diurnal variation of hematological parameters in black rat snake (Pantherophis obsoletus)

Veterinarski glasnik ◽

10.2298/vetgl190506004b ◽

2020 ◽

Vol 74 (1) ◽

pp. 58-67 ◽

Cited By ~ 1

Author(s):

Maja Belic ◽

Maja Lukac ◽

Nika Dvojkovic ◽

Tena Galesic ◽

Mirna Robic ◽

...

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Preliminary Evaluation ◽

Corpuscular Hemoglobin ◽

Black Rat ◽

Black Rat Snakes ◽

Rat Snake

Introduction. The purposes of this study were to determine the presence of daily variations in hematological parameters of the black rat snake (Pantherophis obsoletus) and to compare them with the reference interval values of its subspecies, yellow rat snake (Pantherophis obsoleta quadrivittata). Materials and Methods. Blood from four black rat snakes was collected in the morning and in the evening, and after each collection, blood smears, packed cell volume (PCV), red blood cell count (RBC) and hemoglobin concentration (HGB) were determined, while erythrocyte indices of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were calculated and differential leukocyte counts were evaluated. Results and Conclusions. There were no statistically significant differences in any of the blood parameters in the morning and in the evening. Most of the complete blood count values in the black rat snakes were lower, but still within the reference range of those found in the literature for the yellow rat snake. The results of hematological parameters of the black rat snake could be beneficial for further research of physiological and pathological variations in complete blood count of these snakes, for diagnosing health conditions and for detecting various diseases.

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The Effect of One Bout of Intensive Judo Exercise on Select Hematological and Immunological Parameters in Adolescent Elite Judo Athletes

Asian Journal of Sports Medicine ◽

10.5812/asjsm.101364 ◽

2020 ◽

Vol 11 (2) ◽

Author(s):

Mohammad-Reza Hazratian ◽

Hossein Taheri-Chadorneshin ◽

Asmat Rashidi

Keyword(s):

Blood Cell ◽

Hemoglobin Concentration ◽

Blood Parameters ◽

Adolescent Male ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Immunological Parameters ◽

Distribution Width ◽

Humoral Immune ◽

Paired Samples

Background: Exercise-related studies do not agree on changes in blood parameters regarding anemia and serum immunoglobulin concentration as adaptive immunity. Objectives: The current study aimed to investigate exercise-induced variations in hematological and immunological parameters in response to one bout of intensive judo exercise in adolescent elite judoka. Methods: Venipuncture was performed on ten adolescent male Iranian elite judoka (age: 15.60 ± 0.69 years; body mass index: 24.15 ± 2.80 kg/m2) before and immediately after one bout of intensive judo protocol. Erythrocyte variables [red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), red distribution width (RDW), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)], platelets variables [platelet count (PLT), platelet distribution width (PDW), mean platelet volume (MPV), plateletcrit (PCT)], cellular immune variables [white blood cell (WBC), neutrophil (NEU), lymphocyte (LYM)], and humoral immune variables (IgM, IgA, IgG, IgE) were measured. Data were analyzed using Wilcoxon and paired-samples t-test, and P < 0.05 was considered significant. Results: After the plasma volume was corrected using the Dill and Costill equation, the results showed that RBC, HGB, HCT, MCHC, IgA, and IgM decreased significantly immediately after exercise. However, MCH, RDW, PLT, PDW, MPV, PCT, WBC, LYM, NEU, IgG, and IgE did not change significantly. Conclusions: According to the findings, it can be concluded that intensive judo exercise results in transient anemia and suppression of humoral immune response among adolescent elite judoka.

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