THE STATUS OF RECYCLING OF ORGANIC WASTE ON THE POULTRY FARM «MIKHNEVSKAYA»

Author(s):  
Yu. M. Subbotina ◽  

The article deals with the problem of recycling in poultry farm Mikhnevskaya. The work program provided for the continuation of studies on the development of the system of pond preparation of sewage water in biological ponds of purification. As one of the options, the systems of BOCS ponds (biological oxidation contact stabilization ponds) and ponds with higher aquatic vegetation, as a system of posttreatment of wastewater, as well as the use of fish ponds were proposed. The development of devices that provide deeutrophication of decontaminated wastewater in the BOCS ponds was started. As measures that contributed to the improvement of water purification to be discharged into the environment, an attempt was made to introduce at the final stages of purification of the algobacterial complex, higher aquatic vegetation, and fish seed material. A survey of the poultry farm was conducted; the conditions of wastewater formation of the Mikhnevskaya poultry farm of Stupinsky district of the Moscow region were studied. Model experiments were carried out at the Mikhnevskaya poultry farm in laboratory and semi-production conditions, directly in the laboratory of the Institute at the facility. In the course of experimental studies it was found that in the ponds under the influence of algological complex of microalgae was the process of purification and disinfection of poultry wastewater. The total death of pathogenic bacteria of the E. coli group occurred on 6-11 days after the introduction of microalgae into the water of the algal pond, which does not contradict the existing developments. At the same time, there was still an increase in fish seed material, which was sufficiently provided with food and by the autumn reached a mass of 410 grams.

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
O Klyachenko ◽  
◽  
I Shliakhtun ◽  

National Nature Park “Pyryatynsky” is a valuable reserve of flora and vegetation of the Left-Bank Dnipro. The extensive hydrological network of the Udai River and the wide representation within this nature reserve of floodplain reservoirs are the reason for the high diversity of plant communities of higher aquatic vegetation. In this article we classified the communities of order Callitricho-Batrachietalia in National nature park "Pyryatynskyi" and identify the features of their syntaxonomic and ecological differentiation. Fragmentary and non-comlete information about this type of vegetation are existed in literature, however, without geobotanical releves and detailed characteristics of the structure of phytoceonoses, synecology and synchorology. All obtained results based on original field data. In total, 22 geobotanical releves were performed during the period 2010–2017. The description of communities was carried out within their natural boundaries. Treatment of fitosociological data was performed with the JUICE software package. The nomenclature of syntaxons was consistent with the International Code of Phytosociological Nomenclature (ICFN).The order of Callitricho-Batrachietalia on the territory of NPP "Pyriatynsky" is represented by alliance Batrachion aquatilis Gehu 1961 and 4 associations (Batrachietum aquatilis Gehu 1961, Potameto perfoliati–Batrachietum circinati Sauer 1937, Hottonietum palustris Sauer 1947, Veronico beccabungae–Callitrichetum stagnalis (Oberdorfer 1957) Th. Müller 1962). This is first prodrome of order Callitricho-Batrachietalia for territory of National Nature Park “Pyryatynskyi”. The communities of this syntaxon occupy small areas and have a limited distribution in the region. Most of them are rare and vulnerable to changes in environmental conditions. Monitoring of their structure, chorology and dynamics is an important task to maintain and preserve the species and coenotic diversity of NPP "Pyryatynsky".


2020 ◽  
Vol 10 (1) ◽  
pp. 43-52
Author(s):  
Tran Vu Phuong ◽  
Dang Thi Ngoc Thanh ◽  
Cao Ngoc Diep

Antibiotic has frequently been used in the shrimp-farming process in Vietnam. This leads to the status that antibiotic-resistant bacteria and products do not receive in the market. Bacteria had the resistant ability to pathogenic bacteria in water, and they have an important role in sustainable aquaculture. This study aimed to isolate and select good bacterial strains against Vibrio parahaemolyticus, pathogenic bacteria, on shrimp from 8 samples of shrimp pond water at 3 villages Ngu Lac, Phuoc An and Long Toan of Duyen Hai district, Tra Vinh province on NB agar medium. As a result, fifty-nine bacterial isolates were isolated and 10/59 isolates (16.95%) were identified as resistant to Vibrio parahaemolyticus by the well diffusion method. In 10 isolates, there were 7 isolates had good resistance to select for PCR technique and sequencing. The result indicated that these seven strains, including DH1m, DH2f, DH4d, DH8i, DH8m, DH8n, belonged to Bacilli and DH1n strain belonged to Streptomyces sp.


Author(s):  
Zhanna Sergeevna Makakhaniuk ◽  
Valentina Mikhailovna Zubkova ◽  
Lyubov Anatolievna Rozumnaya

The article describes the purpose of the work, which is determining the distribution and relationships between the composition of pollutants in coastal soil, bottom sediments and higher aquatic vegetation in the small river Khodtsa. There have been presented the data of the laboratory research based on the results of the field work onselecting samples, on concentration of toxicants (cadmium, lead, iron, nitrogen, ammonium and petroleum products) in deposit media, as well as in Carex aquatilis plants of the small river Hodza. This plant species is limiting throughout the river due to the low intensity of biological absorption. The studies were carried out during the summer low water season in 2019 on the river section from the source (Elektrostal city) to the river mouth in the area of Pavlovsky Posad. In all the studied objects there have been found increased concentrations of iron. In the coastal ground and bottom sediments the pollution with heavy metals is manifested to a much greater extent: at the source and intermediate point. Petroleum products and ammonia nitrogen are distributed unevenly in space. A correlation analysis of the experimental data obtained in the components of the environment of the studied territory (the Khodtsa river) was performed. In general, the highest measurement results are observed in the upper reaches of the small river Khodtsa in the industrial city of Elektrostal. The obtained data on the heavy metals migration in the system “сoastal ground – bottom sediments – higher aquatic vegetation” of the small river Khodtsa can be successfully used as indicators an environmental monitoring system and serve as the basis for organizing further research, as well as for developing a strategy for environmental management and environmental protection in the Moscow Region.


2018 ◽  
Vol 29 (2) ◽  
Author(s):  
N. O. Roshchyna

In this article, the current and former distribution of higher aquatic vegetation has been analyzed for floodplain lakes, arenas lakes and third terraces lakes in the valleys of large and medium North-Steppe Dnieper rivers. The article is devoted to the current state analysis of the higher aquatic vegetation at North-Steppe Dnieper lakes, its dynamics over a long-term period, as well as the determination of the nature and extent of anthropogenic-climatic changes in vegetation. Anthropogenic influence is a major threat to the development and functioning of most aquatic ecosystems. Since the twentieth century, it has been intensified by trends to long-term climate changes, which are also largely result of human activity. Increasing temperature of the winter season does not contribute to snow accumulation. Reduction of snow accumulation (frequent thaws during the winter), regulation of river flow (formation of a reservoirs cascade and ponds) and accumulation of melt water in artificial reservoirs led to the smoothing of the peak of the spring flood. Thus, the factor that provided spring washing of floodplain lakes, limited their overgrowing by air-water vegetation and their waterlogging disappeared. The anthropogenic factors that influence negatively include: intensification of agriculture, plowing of coastal areas, unreasonable land reclamation, overgrazing, development of transport and engineering infrastructure, urbanization, recreation, and chemical pollution. The presented data was obtained on the basis of processing our own research materials of 2009–2018 and literary and archival materials analysis (the herbarium of the Dnipropetrovs’k National University and the archive of the Research Institute of Biology). Natural Northern Steppe Dnieper lakes are located mainly in river valleys, so the study area was conventionally divided into sections: the large river valley (Dnieper) and the middle rivers valleys (Samara and Orel). Three ecological groups of macrophytes were reviewed and compared: hydatofites (submerged species), pleistophytes (species with floating leaves) and helophytes (air-water species). The vegetation of Dnieper floodplain lakes practically did not change for all three formation groups. The number of immersed plants communities within the floodplains of medium-sized rivers has decreased by three. The pleistophytes and helophytes associations decreased to fragments of associations. The lakes vegetation within the sandy Dnieper terrace practically did not change for all three formation groups. The submerged lakes plants associations within the sandy medium-sized rivers terraces have been reduced by two. As part of the lakes vegetation on the Dnipro saline terraces, fragments of associations of the two species are considered extinct. A new association of southern adventive species Ruppia maritima L. has appeared within the limits of the middle rivers saline terrace. Changes in higher aquatic vegetation are characteristic of all types of lakes. Changes occur in the direction of crowding out higher aquatic vegetation communities by airborne plant communities. The consequence of the anthropogenic-climatic transformation of aquatic ecosystems is increased mineralization, siltation, and, as a result, intensive overgrowing of lakes by aboriginal and adventive species with a wide ecological amplitude (replacement of sensitive to environmental changes species).


2021 ◽  
Author(s):  
Senjuti Saha ◽  
Chidozie D. Ojobor ◽  
Erik Mackinnon ◽  
Olesia I. North ◽  
Joseph Bondy-Denomy ◽  
...  

ABSTRACTMost Pseudomonas aeruginosa strains produce bacteriocins derived from contractile or non-contractile phage tails known as R-type and F-type pyocins, respectively. These bacteriocins possess strain-specific bactericidal activity against P. aeruginosa and likely increase evolutionary fitness through intraspecies competition. R-type pyocins have been studied extensively and show promise as alternatives to antibiotics. Although they have similar therapeutic potential, experimental studies on F-type pyocins are limited. Here, we provide a bioinformatic and experimental investigation of F-type pyocins. We introduce a systematic naming scheme for genes found in R- and F-type pyocin operons and identify 15 genes invariably found in strains producing F-type pyocins. Five proteins encoded at the 3’-end of the F-type pyocin cluster are divergent in sequence, and likely determine bactericidal specificity. We use sequence similarities among these proteins to define 11 distinct F-type pyocin groups, five of which had not been previously described. The five genes encoding the variable proteins associate in two modules that have clearly re-assorted independently during the evolution of these operons. These proteins are considerably more diverse than the specificity-determining tail fibers of R-type pyocins, suggesting that F-type pyocins emerged earlier or have been subject to distinct evolutionary pressures. Experimental studies on six F-type pyocin groups show that each displays a distinct spectrum of bactericidal activity. This activity is strongly influenced by the lipopolysaccharide O-antigen type, but other factors also play a role. F-type pyocins appear to kill as efficiently as R-type pyocins. These studies set the stage for the development of F-type pyocins as anti-bacterial therapeutics.IMPORTANCEPseudomonas aeruginosa is an opportunistic pathogen that causes a broad spectrum of antibiotic resistant infections with high mortality rates, particularly in immunocompromised individuals and cystic fibrosis patients. Due to the increasing frequency of multidrug-resistant P. aeruginosa infections, there is great interest in the development of alternative therapeutics. One alternative is protein-based antimicrobials called bacteriocins, which are produced by one strain of bacteria to kill other strains. In this study, we investigate F-type pyocins, bacteriocins naturally produced by P. aeruginosa that resemble non-contractile phage tails. We show that they are potent killers of P. aeruginosa, and distinct pyocin groups display different killing specificities. We have identified the probable specificity determinants of F-type pyocins, which opens up the potential to engineer them to precisely target strains of pathogenic bacteria. The resemblance of F-type pyocins to well characterized phage tails will greatly facilitate their development into effective antibacterials.


2020 ◽  
Vol 10 (1) ◽  
pp. 114-121 ◽  
Author(s):  
P. M. Gavrilin ◽  
N. V. Alekseeva ◽  
E. G. Gavrilina ◽  
D. A. Korshenko ◽  
N. V. Kalashnik ◽  
...  

Experimental studies were conducted on the basis of the Research Center for Biosafety and Environmental Control of Resources of the Agroindustrial Complex of the Dnipro SAEU, poultry farms of different directions of productivity of Donetsk-Prydniprovsky region of Ukraine in the period from 2016 to 2019. The purpose of the work is to determine the nature of pathomorphological changes and the status of systemic immunity in chickens with respiratory mycoplasmosis at poultry farms with various production. To determine the immunodeficiency and immunopathological conditions of a productive poultry with respiratory mycoplasmosis, a comprehensive immunological study was performed. Significant fluctuations in the absolute and relative values of leukograms and immunograms have been established, indicating a significant immunological alteration in chickens with respiratory mycoplasmosis. The most significant changes in the status of systemic immunity are found in chickens with respiratory mycoplasmosis at poultry farms with meat production, which are subject to more longer and more intense influence of human activity.


2020 ◽  
Vol 61 (2) ◽  
pp. 165-174 ◽  
Author(s):  
I.L. Rogovskii ◽  
L.L. Titova ◽  
V.I. Trokhaniak ◽  
L.I. Marinina ◽  
O.T. Lavrinenko ◽  
...  

The article analyses the universalization of the seed material by its physical and mechanical properties by means of pre-sowing treatment, resulting in the formation of an artificial shell. As a result of the generalization, a technological scheme of obtaining an encapsulated seed and a design of a seed coating machine was proposed. A simulation model of the sowing accuracy process from the internal friction coefficient of seeds at different root mean square deviations of seed sizes is proposed. The regression equation for the influence of the dynamic mode of operation of the developed experimental sample of seed coating machine is established. According to the experimental studies’ results, the static and dynamic friction coefficients of the encapsulated vegetable seeds on the steel and plastic working surfaces of seed coating machine were established. Under the production conditions, experimental tests were conducted to compare the seedlings of untreated, coated, branded and encapsulated seeds of vegetables by the quality of prepared seed material and sowing time.


2021 ◽  
Author(s):  
Lauren Petrullo ◽  
Tiantian Ren ◽  
Martin Wu ◽  
Rudy Boonstra ◽  
Rupert Palme ◽  
...  

Gut microbiome diversity plays an important role in host health and fitness, in part through the diversification of gut metabolic function and pathogen protection. Elevations in glucocorticoids (GCs) appear to reduce gut microbiome diversity in experimental studies, suggesting that a loss of microbial diversity may be a negative consequence of increased GCs. However, given that ecological factors like food availability and population density may independently influence both GCs and microbial diversity, understanding how these factors structure the GC-microbiome relationship is crucial to interpreting its significance in wild populations. Here, we used an ecological framework to investigate the relationship between GCs and gut microbiome diversity in wild North American red squirrels (Tamiasciurus hudsonicus). We found that higher GCs predicted lower gut microbiome diversity and an increase in metabolic taxa. In addition, we identified a loss of potentially pathogenic bacteria with increasing GCs. Both dietary heterogeneity and an upcoming masting event exhibited direct effects on gut microbiome diversity, whereas conspecific density and host reproductive activity impacted diversity indirectly via changes in GCs. Together, our results suggest that GCs coordinate the effects of ecological change and host biology on gut microbiome diversity, and highlight the importance of situating the GC-microbiome relationship within an ecological framework.


2003 ◽  
Vol 56 (6) ◽  
pp. 553-613 ◽  
Author(s):  
Tamer M Wasfy ◽  
Ahmed K Noor

The status and some recent developments in computational modeling of flexible multibody systems are summarized. Discussion focuses on a number of aspects of flexible multibody dynamics including: modeling of the flexible components, constraint modeling, solution techniques, control strategies, coupled problems, design, and experimental studies. The characteristics of the three types of reference frames used in modeling flexible multibody systems, namely, floating frame, corotational frame, and inertial frame, are compared. Future directions of research are identified. These include new applications such as micro- and nano-mechanical systems; techniques and strategies for increasing the fidelity and computational efficiency of the models; and tools that can improve the design process of flexible multibody systems. This review article cites 877 references.


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