Effect of low-intensity laser radiation of various ranges on the morphology of red blood cells

2020 ◽  
Vol 1 (8) ◽  
pp. 78-83
Author(s):  
A. V. Deryugina ◽  
◽  
M. N. Ivashchenko ◽  
A. A. Belov ◽  
V. A. Petrov ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Forms ◽  
The Body ◽  
Continuous Mode ◽  
Low Intensity ◽  
Negative Effect ◽  
Intensity Laser ◽  
Technological Stress

It is shown that under the influence of technological stress on the body of cattle, there is a violation of the ratio of normal forms of red blood cells – discocytes and their pathological forms – stomatocytes, echinocytes, the most common are echinocytes. To correct impaired body functions, the effect of continuous and fractional exposure to low-intensity laser radiation with wavelengths of 830 nm and 650 nm was studied. The obtained data indicate that the NILI with a wavelength of 650 nm and 830 nm does not have a negative effect on the morphology of bovine red blood cells. The stimulating and normalizing effect of low-intensity laser radiation on red blood cells was most pronounced in a continuous mode of exposure with a wavelength of 830 nm.

scholarly journals Coherent and monochromatic irradiation changes the erythrocyte hemolysis time and the speed of the ion counter transport through the erythrocyte membrane

2020 ◽  
pp. 109-127
Author(s):  
O. B. Almazova ◽  
◽  
M. L. Lysychenko ◽  
Keyword(s):  
Ion Transport ◽  
Red Blood Cells ◽  
Erythrocyte Membrane ◽  
Blood Cells ◽  
The Body ◽  
Effective Thickness ◽  
Blood Samples ◽  
Low Intensity ◽  
Counter Ion ◽  
Intensity Laser

The erythrocytes of human blood were sequentially irradiated with a low-intensity laser (λ = 640 nm), a violet LED (λ = 400 nm), a green LED (λ = 540 nm), and a yellow LED (λ = 592 nm). The method of acid (chemical) erythrograms and the method of counter ion transport compared the kinetic characteristics in irradiated and unirradiated blood samples. Received: - by the method of acid erythrograms it was found that in the irradiated blood samples there is a decrease in the time of hemolysis; - low intensity laser radiation, as well as the emission of LEDs, increase the rate of counter ion transport through red blood cells. - a decrease in the time of hemolysis and an increase in the rate of counter ion transport of irradiated blood samples is due to a decrease in the "effective" thickness of the near-membrane diffusion layer - an immiscible layer of water adjoined to the erythrocyte membrane. A decrease in the "effective” thickness of the near-membrane water layer (minimal in the wavelength range of 570-590 nm and 630-640 nm) changes the rate of metabolic processes in the "cell - intercellular medium" system, changing the mode of cell functioning. The altered mode of functioning is a biological response to light radiation. Red blood cells with an altered mode of functioning are signals - stimuli that cause the body to mobilize resources to fight pathology. These circumstances can predict the creation of a universal phototherapeutic equipment for extracorporeal blood irradiation based on light-emitting diodes with certain exposure parameters.

The Radioprotective Effects of Low-Intensity Laser Radiation on Rat Peripheral Blood Cells

BIOPHYSICS ◽  
2019 ◽  
Vol 64 (3) ◽  
pp. 463-469
Author(s):  
G. A. Zalesskaya ◽  
V. M. Nasek ◽  
R. D. Zilberman
Keyword(s):  
Laser Radiation ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Low Intensity ◽  
Intensity Laser

MORPHOFUNCTIONAL PARAMETERS OF CATTLE ERYTHROCYTES UNDER STRESS AND ITS CORRECTION BY LOW-INTENSITY LASER RADIATION

1970 ◽  
pp. 67-71
Author(s):  
М. N. Ivashchenko ◽  
А. V. Deryugina ◽  
P. S. Ignatiev ◽  
V. B. Metelin ◽  
М. N. Talamanova ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Erythrocyte Membrane ◽  
Control Level ◽  
Experimental Studies ◽  
Erythrocyte Membranes ◽  
Physical Factors ◽  
Low Intensity ◽  
Intensity Laser ◽  
Technological Stress

The study investigated the content of malondialdehyde (MDA), the electrophoretic mobility of erythrocytes (EPME), the spectrum of erythrocyte membrane proteins and the morphology of cattle erythrocytes in in vitro experiments under stress and exposure to low-intensity laser radiation (LLLT). Clinical and experimental studies carried out in the last decade indicate the possibility of modulating the organism adaptive reactions when exposed to such physical factors as low-intensity laser radiation. The work showed that the effect of LLLT on the blood of stressed animals caused the restoration of the studied parameters to the level of physiological norms, while in animals that underwent technological stress, EPME was reduced by 31%, MDA concentration was increased by 65%. The effect of LLLT on the blood of unstressed animals did not lead to a change in EPME and MDA concentration. The study of the protein spectrum of erythrocyte membrane of animals subjected to technological stress revealed that the content of spectrin decreased by 16%, glycophorin C increased by 35%, the morphology of erythrocytes after stress was characterized by an increase in the number of echinocytes, stomatocytes and degeneratively altered erythrocytes. Under the LLLT action on the cow erythrocytes after stress there was a restoration of the morphology of cells and the content of proteins of erythrocyte membranes to the control level.

scholarly journals Evaluation of some biochemical indicators of blood of high-qualified short-track sportsmen under the combined influence of specific physical load and low-intensive laser radiation

2018 ◽  
Vol 20 (1) ◽  
pp. 108-110
Author(s):  
K A Strelycheva ◽  
T M Bruk ◽  
N V Osipova
Keyword(s):  
Laser Radiation ◽  
Lactate Concentration ◽  
Individual Characteristics ◽  
The Body ◽  
Physical Load ◽  
Blood Protein ◽  
Total Blood ◽  
Protein Levels ◽  
Low Intensity ◽  
Intensity Laser

Monitoring of biochemical parameters of blood allows to determine the individual characteristics of athletes’ body and evaluate the effectiveness of building a training process. The increasing volume of physical activity during the preparatory period forces us to search for means of rapid increase in the functional level state and physiological reserves of an organism. In this regard, as one of the methods, the physiotherapeutic course effect of low-intensity laser radiation can be used. It was revealed that a specific physical load leads to a significant increase in lactate and total blood protein levels. So, in short-trackers in a state of relative physiological rest, the concentration of lactate was 2,77±0,24 mmol/l, which is much higher than the norm (the norm is 1,3±0,35 mmol/l). After the course of low-intensity laser radiation, the lactate level decreased insignificantly (2,52±0,19 mmol/l), but did not reach normal values. After the action on the body of a specific physical load, lactate concentration increased significantly, reaching a level equal to 9,24±0,43 mmol/l. The conducted course laser therapy allowed to reliably (р 0,05) lower its level to 6,25±0,32 mmol/l. In this case, urea and creatinine had only a tendency to increase. Thus, low-intensity laser radiation against the background of a specific physical load makes it possible to bring the investigated parameters close to the boundary of the physiological norm.

scholarly journals CORRECTION OF TECHNOLOGICAL STRESS BY LOW-INTENSITY LASER RADIATION

2021 ◽  
Author(s):  
Belov A. A. ◽  
◽  
A.V. Deryugina ◽  
M.N. Ivashchenko ◽  
◽  
...  
Keyword(s):  
Laser Radiation ◽  
Environmental Factors ◽  
Metabolic Disorders ◽  
Meat Products ◽  
Animal Husbandry ◽  
Cell Level ◽  
Low Intensity ◽  
Intensity Laser ◽  
Technological Stress

Intensive industrial technologies in animal husbandry lead to an increase in cases of stress and metabolic disorders, which affects the quality of dairy and meat products. It is important to increase the resistance of animals to environmental factors by non-drug methods. The aim of the work is to study the effect of technological stress and low-intensity laser radiation at the cell level.

COMBINED ACTION OF LOW-INTENSITY LASER RADIATION AND AUTOLOGOUS PLASMA WITH BLOOD CELLS ON THE MICROCIRCULATORY APPARATUS OF THE SKIN DURING INVOLUTION CHANGES

2021 ◽  
pp. 983-988
Author(s):  
Ж. Ю. Юсова ◽  
Л. С. Круглова
Keyword(s):  
Laser Radiation ◽  
Blood Cells ◽  
Clinical Signs ◽  
Autologous Plasma ◽  
Skin Changes ◽  
Low Intensity ◽  
Vascular Component ◽  
Combined Action ◽  
Mixed Types ◽  
Intensity Laser

Инволюционные изменения кожи связаны с динамикой показателей его микроциркуляторного аппарата, среди которых выделены спастический, атонический и смешанный типы. Изучали комбинированное влияние низкоинтенсивного лазерного излучения и аутологичной плазмы с клетками крови на сосудистый компонент кожи. В свете восстановления микруциркуляторного аппарата кожи при различных типах анализировали клиническую эффективность проводимой терапии. Изучение эффективности комбинированного применения низкоинтенсивного лазерного излучения и аутологичной плазмы с клетками крови проводили на 57 пациентах с клиническими признаками инволюционного изменения кожи. В зависимости от типа микроциркуляторных изменений кожи все пациенты были разделены на три сопоставимые группы: 1-я - атонический тип ( n =24);2-я - спастический ( n =12); 3-я - смешанный ( n =21).Комплексная оценка динамики показателей микроциркуляции кожи, особенностей пассивного и активного кровотока свидетельствует о значимо выраженном положительном комбинированном влиянии низкоинтенсивного лазерного излучения и аутологичной плазмы с клетками крови у пациентов со спастическим типом микроциркуляции. Involutional changes of the skin are associated with changes in its microcirculatory apparatus, among which spastic, atonic and mixed types are distinguished. The effect of low-intensity laser radiation in combination with autologous plasma with blood cells on the vascular component of the skin was studied. In the light of the restoration of the microcirculatory apparatus of the skin in different types, the clinical efficacy of the therapy was analyzed. The study of the effectiveness of low-intensity laser radiation in combination with autologous plasma with blood cells was conducted in 57 patients with clinical signs of involutive skin changes. Depending on the type of microcirculatory skin changes, all patients were divided into 3 comparable groups: the 1- atonic type ( n =24), the 2- spastic ( n =12), the 3- mixed ( n =21).The obtained data of a complex assessment of the dynamics of skin microcirculation, features of passive and active blood flow, indicate a significant pronounced positive effect of low-intensity laser radiation in combination with autologous plasma with blood cells in patients with spastic type of microcirculation.

scholarly journals Peculiarities of the body of a young sheep under biophysical methods of exposure

2021 ◽  
Vol 13 ◽  
pp. 14-24
Author(s):  
M.A. Afanasyev ◽  
◽  
S.N. Shlykov ◽  
S.S. Bobryshov ◽  
R.S. Omarov ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Infrared Region ◽  
The Body ◽  
Breast Development ◽  
Control Group ◽  
Low Intensity ◽  
Group I ◽  
Significant Difference ◽  
The Difference ◽  
Intensity Laser

Aim. Study the effect of low-intensity laser radiation on the genotype's young sheep (poll Dorset x North Caucasian meat-wool) and their exterior. Material and Methods. Zootechnical, biophysical, statistical and other methods were used in research. Experimental animals were exposed to an STP laser apparatus, the action of which is based on the use of low-intensity laser radiation in the infrared region of the spectrum. The study of measurements of animals at the age of 5 and 7 months was carried out and physique indices were calculated. Results. Comparing measurements in the studied animals at 5 months of age revealed the advantage of lambs of groups II and III over the control, in almost all main parameters. However, the experimental groups' young animals were characterized by higher rates of breast measurements. Thus, the superiority of lambs of groups II and III over animals of the control group were: in chest depth 2.1-3.3%, width – 4.4-7.1%, girth – 1.2-4.1% (P < 0.001). A similar trend was established due to measurements of the studied animals' physique at the age of 7 months. At the same time, a clear advantage in terms of measurements characterizing breast development was found in young animals of the second and, especially, in the third group: in chest depth by 3.8-7.4%, width by 4.8-9.1%, girth – 3,7-9.0%, with a significant difference (P < 0.05; P < 0.001). It was also found that the greatest value of the breast index at 5 months of age was typical for animals of groups II and III (77.6-78.6%), which is 1.8-2.8 abs. percent (P < 0.001). However, with age, the difference in the value of this indicator between the groups decreased. Therefore, at the age of 7 months, young animals of groups II and III surpassed animals of group I in this indicator by 0.8-1.5 abs. percent. Conclusion. Thus, it was found that the best ratio of most body articles and body build indices, characterizing them as animals with well-pronounced meat forms, were animal groups II and III.

Sign in / Sign up

Export Citation Format

Share Document