SENSITIVITY OF S+ERUM PROTEINS OF GI CANCER PATIENTS TO CHEMOTHERAPY COURSES

Gastrointestinal cancers (GI) are one of the most abundant types of cancers among the world population, though statistical data indicate that in eastern Asia these types of cancer occur 4 times more often than in Western Europe. Absence of treatment of bacterial infections, obesity, and lack of vegetable food in a diet can be the case of GI cancer. All pathologies are inevitably connected to the changes in cell cycle, abnormal protein amount and their dysfunction. Serum proteins are widely used as an additional source of information about body condition, also changes in protein composition can point out the mechanism of disease development and effectiveness of treatment. In the presented work we studied protein composition of GI cancer patients in different stages of cancer development, after and before chemotherapy and compared these data to protein composition of healthy control group of voluntaries. Treatment of patients was performed according the guidelines appropriate for the GI cancer. Association of the effectiveness of treatment at the different stages of chemotherapeutic courses and changes of protein composition of blood serum has been assessed. Proteins composition was studies by SDS-PAGE electrophoresis and densitometry analysis. Experimentally gained molecular and statistical information exposed the most vulnerable groups of proteins affected by chemotherapeutic agents indicating targets for searching new biomarkers for treatment effectiveness. Research involving human patients performed in accordance with the requirements of the Council of Europe Convention on Human Rights and Biomedicine, Biomedical Research, as well as the UNESCO Declaration of Bioethics and Human Rights. Keywards: Gastrointestinal cancer, chemotherapy, proteins, biomarkers.

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The Effects of Individual Diet Therapy on Food Intake, Quality of Life, and Related Serum Proteins in Patients with Breast Cancer: A Randomized Clinical Trial

Basic & Clinical Cancer Research ◽

10.18502/bccr.v12i3.5768 ◽

2021 ◽

Author(s):

Azadeh Fallah ◽

Kiana Parnian ◽

Hamid Abdolazimi ◽

Sajjad Tezerji ◽

Zohreh Mazloom

Keyword(s):

Breast Cancer ◽

Quality Of Life ◽

Side Effects ◽

Cancer Patients ◽

Serum Proteins ◽

Intervention Group ◽

Diet Therapy ◽

Control Group ◽

Nutritional Needs

Background: In cancer patients, weight loss due to malnutrition has a significant impact on the patients’ treatment and quality of life. This study aimed to determine the appropriate therapeutic strategy to control the side effects of chemotherapy in patients with breast cancer to improve their health, quality of life, and nutritional status. Methods: In our prospective study, we examined gastric cancer patients who were Seventy patients undergoing chemotherapy were included and randomly divided into intervention (n=35) and control groups (n=35). The intervention group received an individualized diet according to their nutritional needs for eight weeks, and the control group received dietary advice on the side effects of chemotherapy. Malnutrition, nutritional barriers, and patients’ quality of life were evaluated by PG-SGA, nutritional barriers, and QLQ-C30 questionnaires. Serum proteins were also assessed at the beginning and the end of the study. Results: The patients’ mean age was 50.91±1.72 years in the intervention group and 51±1.35 in the control group. According to the PG-SGA questionnaire classification, 68.5% of patients had malnutrition at baseline. In the intervention group, the mean score of PG-SGA decreased, which indicated an improvement in patients’ nutritional status. Increased scores in the functional section of QLQC30 and a decrease in the symptom section of this questionnaire indicated the improved quality of life in patients undergoing treatment at the end of the intervention. Albumin (P<0.001) and hemoglobin (P<0.001) levels increased in the intervention group, while there were no significant changes in these variables of the control group. Serum levels of ferritin did not show significant changes in either the intervention or the control group. Conclusion: Identifying nutritional barriers in breast cancer patients and individual diet therapy based on these barriers and nutritional needs reduces nutritional barriers. Consequently, malnutrition would decline, and the quality of life may enhance in these patients.

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Analysis of protein compounds in old persons whole saliva with parodontal diseases

Stomatoloski glasnik Srbije ◽

10.2298/sgs0202029m ◽

2002 ◽

Vol 49 (1-2) ◽

pp. 29-33 ◽

Cited By ~ 1

Author(s):

Silvija Mukovic ◽

Obrad Zelic ◽

Biljana Andjelski

Keyword(s):

Periodontal Disease ◽

Serum Proteins ◽

Protein Composition ◽

Human Organism ◽

Control Group ◽

Electrophoretic Method ◽

Old Patients ◽

Whole Saliva ◽

Periodontal Index ◽

Alkaline Conditions

The purpose of this work was to determine the protein composition of whole saliva in 50 year-old patients an d older with manifested and terminal stages of periodontal disease, in correlation with control group of young healthy persons. Experimental and control group consisted of 30 patients. The periodontal status was determinate by using appropriate periodontal index (Sillness-Loe). Investigations were led by electrophoretic method on aragose gel which was used as a routine method for investigation of human serum proteins. Investigating in very alkaline conditions (pH-H) as well as acid conditions (pH=5.9) resulted in separating alkaline and acid glycoprotein fractions in whole saliva. Results showed that there were more separated fractions within basic conditions than within acid conditions. The fraction identification was done by using relative molecule mass determination method by Weber and Osborne. Molecule mass fractions at basic conditions in comparison with the healthy sample (Sz) indicated the destruction of big molecular weight alkaline proteins as well as a significant presence of acid fractions, of little molecule masses up to 60 000 (St), consequences due to aging processes in human organism. It can be concluded that changes in protein composition of whole saliva could point out to the direction and development of periodontal disease as well as changes concerning aging of human organism.

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Serum Fructosamine in Uraemia, Myeloma and Acute Inflammatory Disorders—Relationship to Serum Glucose and Albumin Levels

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456328902600109 ◽

1989 ◽

Vol 26 (1) ◽

pp. 63-68 ◽

Cited By ~ 4

Author(s):

D R McCance ◽

K C Clarke ◽

L Kennedy

Keyword(s):

Serum Albumin ◽

Significant Positive Correlation ◽

Serum Proteins ◽

Protein Composition ◽

Serum Glucose ◽

The Other ◽

Control Group ◽

Short Term ◽

Inflammatory Conditions ◽

Serum Fructosamine

There has been considerable interest in the serum fructosamine assay as a measure of glycated serum proteins. We have measured serum fructosamine in three groups of patients—those with uraemia; those with multiple myeloma; and those with acute inflammatory conditions—none of whom were known to have diabetes. Serum fructosamine was significantly higher in the uraemic group than in the other two, and also than in a control group. When allowance was made for prevailing serum albumin levels fructosamine was shown to be increased in the acute inflammatory group also. There was a significant correlation between random plasma glucose and serum fructosamine only when fructosamine was adjusted for prevailing albumin levels. In control and uraemic subjects there was a significant positive correlation between serum fructosamine and albumin levels, whereas in the myeloma group there was a negative correlation with serum protein. These data would suggest the need to take into account serum albumin levels and protein composition if serum fructosamine is accurately to reflect short-term integrated glycaemia.

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The Inflammatory Modulation Effect of Glutamine-Enriched Total Parenteral Nutrition in Postoperative Gastrointestinal Cancer Patients

The American Surgeon ◽

10.1177/000313481107700122 ◽

2011 ◽

Vol 77 (1) ◽

pp. 59-64 ◽

Cited By ~ 8

Author(s):

Chien-Yu Lu ◽

Ying-Ling Shih ◽

Li-Chu Sun ◽

Jui-Fen Chuang ◽

Cheng-Jen Ma ◽

...

Keyword(s):

Cancer Patients ◽

Interleukin 10 ◽

Infectious Complications ◽

Control Group ◽

Modulation Effect ◽

Lower Serum ◽

Inflammatory Status ◽

Gi Cancer ◽

Factor Α ◽

Necrosis Factor

The objective of this study is to explore the inflammatory modulation effect of glutamine-enriched total parenteral nutrition (TPN) by investigating the alterations of inflammation-related cytokines in gastrointestinal (GI) cancer patients postoperatively. Fifty GI cancer patients received postoperative 7 days of isocaloric and isonitrogenous TPN after operation. They were randomly divided to receive either glutamine-enriched TPN or standard TPN. The inflammation-related cytokines including interleukin-6, interleukin-10, and tumor necrosis factor-α were also determined. Records of nutritional assessments, inflammatory status, and postoperative complications were compared between the two groups. Of 50 enrolled patients, 25 patients were classified as the intervention group, and the control group also comprised 25 patients. The differences of gender, age, primary GI malignancies, and hematological and biochemical data between the two compared groups were not statistically significant (all P > 0.05). Compared with standard TPN, a higher serum prealbumin level and better nitrogen balance were observed in glutamine-enriched TPN ( P = 0.039 and 0.048 respectively). A significantly lower serum interleukin-6 level was found in comparing glutamine-enriched with standard TPN ( P = 0.01), but not in interleukin-10 ( P = 0.374) and tumor necrosis factor-α levels ( P = 0.653). Moreover, a significant lower serum C-reactive protein level was detected in glutamine-enriched TPN compared with standard TPN ( P = 0.013). Indeed, four cases of postoperative infectious complications were noted in the control group, but no postoperative infectious complications were observed in the interventional group ( P = 0.037). Our present study shows that glutamine-enriched TPN may be beneficial in improving the inflammatory status and decreasing the infectious morbidity in postoperative GI cancer patients.

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OLANZAPINE COMBINED WITH STANDARD ANTIEMETIC REGIMENS FOR PREVENTION OF CHEMOTHERAPY THERAPY-INDUCED NAUSEA AND VOMITING: A SINGLE-CENTER EXPERIENCE FROM SOUTH INDIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i11.18864 ◽

2017 ◽

Vol 10 (11) ◽

pp. 247 ◽

Cited By ~ 1

Author(s):

Emmanuel James ◽

Drisya Pm ◽

Wesely M Jose

Keyword(s):

Cancer Patients ◽

Negative Impact ◽

Antipsychotic Agent ◽

Chemotherapeutic Agents ◽

Nausea And Vomiting ◽

Control Group ◽

Study Group ◽

European Organization ◽

Single Center Experience ◽

Delayed Nausea

Objectives: Olanzapine, an antipsychotic agent, exhibits significant antiemetic properties due to its inhibitory activity on neurotransmitters at multiple receptors involved in chemotherapy-induced nausea and vomiting (CINV). CINV can have an immensely negative impact on patient’s quality of life (QOL) and daily activities. Our objectives were to determine the effectiveness of adding olanzapine to standard antiemetic regimens for the prevention of CINV in cancer patients and to compare the QOL of such patients with those receiving standard antiemetic regimens.Methods: A prospective, observational, cohort study was done on patients receiving either highly or moderately emetogenic chemotherapy (MEC). The patients who received only the standard antiemetic regimens were considered as the control group and those who received 10 mg of olanzapine once daily on days 1-5 of chemotherapy in addition to the standard antiemetic regimens were considered to be the study group. The patients were assessed for grades of nausea and vomiting using National Cancer Institute common terminology criteria for adverse events and for QOL using European Organization in Research and Treatment of Cancer QOL questionnaire.Results: Patients were evaluated for a total of 168 cycles of chemotherapy. Compared to the control group, the study group patients showed significant improvement in response to acute nausea (p=0.02) but not in acute vomiting (p=0.09). However, response to delayed nausea and vomiting improved significantly (p=0.004 and p=0.05, respectively). The QOL of study group patients showed significant improvement in functional scales and symptom scales (p<0.02). Global health status also increased significantly (p=0.02) in the study group patients.Conclusion: Olanzapine containing pre-medication regimens can reduce acute and delayed nausea and delayed vomiting and improve the QOL of cancer patients receiving highly or moderately emetogenic chemotherapeutic agents as compared to the standard pre-medication regimens.

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Incidence of a split alpha 2-glycoprotein band in the electrophoretic pattern for serum of adenocarcinoma patients.

Clinical Chemistry ◽

10.1093/clinchem/26.3.0392 ◽

1980 ◽

Vol 26 (3) ◽

pp. 392-395 ◽

Cited By ~ 1

Author(s):

G B Dermer ◽

L M Silverman ◽

S J Gendler ◽

Z A Tökés

Keyword(s):

Cancer Patients ◽

Serum Proteins ◽

Acute Phase Proteins ◽

Systemic Disease ◽

Periodic Acid ◽

Electrophoretic Pattern ◽

Control Group ◽

Periodic Acid Schiff ◽

Cellulose Acetate Membranes ◽

Schiff Reagent

Abstract We electrophoresed serum samples on Mylar-backed cellulose acetate membranes and stained for glycoproteins with the periodic acid—Schiff reagent. The samples were from untreated adenocarcinoma patients, adenocarcinoma patients receiving chemotherapy, and patients with other malignancies, and also from patients with benign proliferative diseases, inflammatory diseases, and other non-malignant conditions. Forty-five per cent of the sera from untreated adenocarcinoma patients and 80% of those from adenocarcinoma patients with progressive systemic disease exhibited a splitting of the alpha 2-glycoproteins into a fast and slow band. Such a pattern was seen in only 4% of the non-adenocarcinoma cancer patients and 4% of the control group. Serial studies indicated that electrophoretic patterns of alpha 2-glycoproteins change with clinical status. Non-cancer patients with high concentrations of acute-phase proteins in their serum did not exhibit two alpha 2-glycoprotein bands. Further characterization of serum proteins from the fast alpha 2 region suggest that alpha 1-acid glycoprotein and haptoglobin beta chains are the principal components staining with periodic acid—Schiff reagent. These components are markedly less apparent in, or are absent from, the fast alpha 2 region of normal sera.

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Prevalence of Major Cardiac Events of Anthracycline-Induced Cardiotoxicity in Southwestern Iran: Different Response Patterns to Cumulative Dose

Current Drug Therapy ◽

10.2174/1574885514666190311145607 ◽

2020 ◽

Vol 15 (1) ◽

pp. 78-84

Author(s):

Mahsa Behrouzian ◽

Babak Najibi ◽

Sabahat Haghi ◽

Chehreh Mahdavi ◽

Kaveh Jaseb ◽

...

Keyword(s):

Cancer Patients ◽

Cumulative Dose ◽

Chemotherapeutic Agents ◽

Left Ventricular ◽

Left Ventricular Ejection ◽

Control Group ◽

Ventricular Ejection Fraction ◽

Significant Difference ◽

High Cumulative Dose ◽

And Control

Background: Anthracyclines are widely used chemotherapeutic agents in several cancers. Since its use, survival improved significantly among cancer patients and has been reported to be up to 80%. However, anthracyclines possess several cardiac, renal and hematological toxicities which limit their use in practice. Cardiotoxicity is still the most important and dose-limiting side effect of anthracycline treatment. Here we aimed to investigate the frequency of anthracyclineinduced cardiomyopathy in pediatric malignancies in Khuzestan Province, Iran. Methods: A total of 112 patients were enrolled in the present study. Patients were allocated to the case or control group based on receiving anthracycline. Echocardiographic examinations were performed by a cardiologist. Electrocardiograms were also recorded. Results: We showed that cancer patients who underwent anthracycline treatment showed cardiomyopathy as defined by lower LVEF (Left Ventricular Ejection Fraction) among patients (p = 0.041). Abnormal LVEF was reported with a frequency of about 9.5% in patients (p = 0.026). However, LVFS (Left Ventricular Fraction Shortening), QRS voltage and QT interval did not differ significantly between treatment and control groups. Our data analysis revealed that this difference is mainly related to high cumulative dose since high cumulative dose of anthracycline (>300 mg/m2) leads to lower LVEF and LVFS and higher QRS voltage in comparison with lower cumulative dose (<300 mg/m2) and control group; but there was no significant difference between low dose and control group. Different age groups and type of malignancy including hematological and solid tumors did not show any significant differences for echocardiographic and electrocardiograms parameters. Conclusion: In our study, lower LVEF among patients who received anthracyclines were mainly related to a high cumulative dose of anthracyclines, which emphasizes the effect of cumulative dose for cardiotoxic effects. Larger studies are needed to investigate possible other risk factors for cardiotoxicity.

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Incidence of a split alpha 2-glycoprotein band in the electrophoretic pattern for serum of adenocarcinoma patients.

Clinical Chemistry ◽

10.1093/clinchem/26.3.392 ◽

1980 ◽

Vol 26 (3) ◽

pp. 392-395 ◽

Cited By ~ 1

Author(s):

G B Dermer ◽

L M Silverman ◽

S J Gendler ◽

Z A Tökés

Keyword(s):

Cancer Patients ◽

Serum Proteins ◽

Acute Phase Proteins ◽

Systemic Disease ◽

Periodic Acid ◽

Electrophoretic Pattern ◽

Control Group ◽

Periodic Acid Schiff ◽

Cellulose Acetate Membranes ◽

Schiff Reagent

Abstract We electrophoresed serum samples on Mylar-backed cellulose acetate membranes and stained for glycoproteins with the periodic acid—Schiff reagent. The samples were from untreated adenocarcinoma patients, adenocarcinoma patients receiving chemotherapy, and patients with other malignancies, and also from patients with benign proliferative diseases, inflammatory diseases, and other non-malignant conditions. Forty-five per cent of the sera from untreated adenocarcinoma patients and 80% of those from adenocarcinoma patients with progressive systemic disease exhibited a splitting of the alpha 2-glycoproteins into a fast and slow band. Such a pattern was seen in only 4% of the non-adenocarcinoma cancer patients and 4% of the control group. Serial studies indicated that electrophoretic patterns of alpha 2-glycoproteins change with clinical status. Non-cancer patients with high concentrations of acute-phase proteins in their serum did not exhibit two alpha 2-glycoprotein bands. Further characterization of serum proteins from the fast alpha 2 region suggest that alpha 1-acid glycoprotein and haptoglobin beta chains are the principal components staining with periodic acid—Schiff reagent. These components are markedly less apparent in, or are absent from, the fast alpha 2 region of normal sera.

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Making Human Rights a Reality

10.23943/princeton/9780691155357.001.0001 ◽

2013 ◽

Cited By ~ 4

Author(s):

Emilie M. Hafner-Burton

Keyword(s):

Human Rights ◽

International Law ◽

World Population ◽

International Human Rights Law ◽

Legal Norms ◽

Human Rights Protection ◽

National Human ◽

The World ◽

Protection Of Human Rights ◽

National Human Rights Institutions

In the last six decades, one of the most striking developments in international law is the emergence of a massive body of legal norms and procedures aimed at protecting human rights. In many countries, though, there is little relationship between international law and the actual protection of human rights on the ground. This book takes a fresh look at why it's been so hard for international law to have much impact in parts of the world where human rights are most at risk. The book argues that more progress is possible if human rights promoters work strategically with the group of states that have dedicated resources to human rights protection. These human rights “stewards” can focus their resources on places where the tangible benefits to human rights are greatest. Success will require setting priorities as well as engaging local stakeholders such as nongovernmental organizations and national human rights institutions. To date, promoters of international human rights law have relied too heavily on setting universal goals and procedures and not enough on assessing what actually works and setting priorities. This book illustrates how, with a different strategy, human rights stewards can make international law more effective and also safeguard human rights for more of the world population.

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