scholarly journals FRAKSI AIR EKSTRAK DAUN WUNGU (Graptopphyllumpictum L.) SEBAGAI ANTIINFLAMASI TERHADAP TIKUS PUTIH JANTAN

2021 ◽  
Vol 9 (1) ◽  
pp. 1-9
Author(s):  
Ramadhan Triyandi ◽  
◽  
Akhmad Rokiban ◽  
Catur Setia Pratiwi MS ◽  
◽  
...  
Keyword(s):  
Leaf Extract ◽  
Diclofenac Sodium ◽  
Water Extract ◽  
Positive Control ◽  
Control Group ◽  
Water Fraction ◽  
White Rats ◽  
Group 3 ◽  
Anti Inflammatory ◽  
Negative Controls

Abstract This study aims to determine the anti-inflammatory effect of the water extract of Daun wungu (Graptophyllum pictum L.) extract which was observed from the decrease in the volume of edema of rat’s feet which was induced by 1 % carrageenan as much as 0,1 ml, was carried out in 25 male white rats divided into 5 groups. Negative controlwas given distilled water given 5 % Na CMC, diclofenac sodium as a positive control with a dose of 0,9 mg/200 g BB and water fraction of wungu leaf extract with a dose 1 (1,125 mg/kg bb), dose 2 (2,25 mg/kg bb) dan dose 3 (4,5 mg/kg bb). The measurement of edema volume is carried out every hour for six hours after induced carrageenan. From the results of testing the water fraction of wungu leaf extract, % inhibition showed that the dose group 3 (4,5 mg/kg bb) had anti-inflammatory activity that was comparable to the positive control group. Of all dosage variatons in this study the effective dose which has the highest percentage of edema is the dose 3 (4,5 mg/kg bb). Based on the results of statistical analysis, the percentage of edema inhibition data on the water fraction of wungu lear extract from all dose variations showed significant differences with negative controls. Keyword : Graptophyllum pictum L Anti-Inflamasi

scholarly journals In Vitro Anti-Inflammatory Assay of Bitter Melon (Momordica charantia L.) Ethanol Extract

2020 ◽  
Vol 7 (3) ◽  
pp. 1-4
Author(s):  
Rahmawati Rahmawati ◽  
Harti Widiastuti ◽  
Eka Sulistya
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Positive Control ◽  
Regression Equation ◽  
Positive Control Group ◽  
Control Group ◽  
Bitter Melon ◽  
Inflammatory Agent ◽  
Anti Inflammatory

Bitter melon contains flavonoids that have anti-inflammatory function. Inflammation can be caused by protein denaturation. This research tested the anti-inflammatory potential of ethanol extract of leaves and bitter melon (Momordica charantia L.) using a UV-Vis spectrophotometer and protein denaturation inhibition method. There were three experimental groups formed in this research including negative control group, positive control group, and test solutions. Diclofenac sodium was used in the positive control group at concentration series of 1, 2, 4, 8, and 16 ppm, obtaining a regression equation Y = 3.546X + 2.163 and r = 0.9990. Whilst, for bitter melon ethanol extract at a series of concentrations of 10, 20, 40, 80 and 160 ppm obtained a regression equation Y = 0.243X + 11.74 and r = 0.9995. The potential of diclofenac sodium as an anti-inflammatory agent was shown by IC50 of 13.490 µg / mL, while the ethanol extract of bitter melon fruit has an IC50 of 157.448 µg / mL. This result indicated that bitter melon ethanol extract had the potential as moderate anti-inflammatory agent.

scholarly journals Effect of Jati Belanda Leaves Extract on Myeloperoxidase Level in Wistar Rat's Lung Induced Contusion Pulmonum

2019 ◽  
Vol 5 (3) ◽  
pp. 116-120
Author(s):  
Nia Savitri Tamzil ◽  
Evi Lusiana ◽  
Desi Oktariana
Keyword(s):  
Leaf Extract ◽  
Positive Control ◽  
Lung Damage ◽  
Control Group ◽  
Alveolar Cells ◽  
Pulmonary Tissue ◽  
Control Group Design ◽  
White Rats ◽  
Guazuma Ulmifolia

Pulmonary contusions are injuries to the lung parenchyma that often result from blunt trauma to the chest wall. This injury will activate the inflammatory response which can produce the effects of oxidative stress so that eventually lung damage occurs. Several studies have identified the effects of Jati Belanda leaves extracts related to the inflammatory process and their effects as antioxidants. This research is an in vivo experimental study with a prepost-test with control group design approach that aims to determine the effectiveness of the extracts of Jati Belanda (Guazuma ulmifolia) in its protection against pulmonary alveolar cells by pretreatment pulmonary contusions. The subjects of this study were white rats (Rattus norvegicus) Wistar strain which were divided into 5 groups namely positive control, negative control, Dutch teak leaf extract 125 mg / kgBB, 250 mg / kgBB and 500 mg / kgBW. Rats were induced by dropping a weight of 400 grams as high as 50 cm. The results of the study of Jati Belanda Leaf Extract (EDJB) all doses and positive control can significantly reduce levels of myeloperoxidase expression (p <0.05). Therefore, it can be concluded that the extracts of Jati Belanda leaves (Guazuma ulmifolia) are all effective doses in reducing the expression of myeloperoxidase in pulmonary tissue of Wistar rats induced by pulmonary contusions.

scholarly journals Hypoglycemic activity test on smooth pigweed (Ammaranthus Hybridus L) leaf water extract on male Wistar rats

2021 ◽  
pp. 56-60
Author(s):  
Asti Yunia Rindarwati
Keyword(s):  
Wistar Rats ◽  
Water Extract ◽  
Positive Control ◽  
Hypoglycemic Activity ◽  
Control Group ◽  
White Rats ◽  
Male Wistar Rats ◽  
Tolerance Method ◽  
Smooth Pigweed

Introduction: Type 2 diabetes mellitus consists of an array of dysfunction characterized by hyperglycemia. The activity of smooth pigweed (Amaranthus hybridus L.) leaves water extract on male Wistar rats. Objectives: This research was started by supplying simplicia, making smooth pigweed leaves water extract, and testing the hypoglycemic activity of smooth pigweed leaves water extract on male Wistar rats. Methods: The glucose tolerance method was used to determine the hypoglycemic activity of smooth pigweed leaves water extract. Male white rats were divided into five groups of six rats each: a positive control group (0.5% of tragacanth suspension), a comparison group (Diabinese suspension at a dose of 22.5 mg/kg body weight (bw)), and three test groups at doses of 50 mg/kg bw, 100 mg/kg bw, and 150 mg/kg bw. Results and conclusions: The most significant hypoglycemic activity was seen with the dose of 150 mg/kg bw in comparison with the control group at 90 minutes.

scholarly journals Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica)  is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

scholarly journals PENGARUH PEMBERIAN SUSU BEKATUL TERHADAP KONSENTRASI KOLESTEROL DAN TRIGLISERIDAPADA TIKUS PUTIH WISTAR DIABETES

2019 ◽  
Vol 12 (02) ◽  
Author(s):  
Husnul Khatimah ◽  
Saifuddin Sirajuddin ◽  
Zainal Zainal
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Group 4 ◽  
White Rats ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

ABSTRACT In diabetics there is usually an increase in free fatty acids in the blood and increases the concentration of cholecerols and triglycerides, this condition is called diabetes dyshidemia. This study aimed to find out at the effect of bran milk treatment on the changes of the concetration of cholesterol and triglycerides in diabetetic white wistar rats. Conducted on May 15 until 15 August 2018. Experimental research pre-post test with control design. Subjects of twenty wistar white rats were made diabetic by injecting alloxan 140mg / kg. After being declared diabetic, the rats were divided into four groups, namely group 1 (negative control), group 2 (positive control), group 3 (bran milk 0.54g / 200 gr) and group 4 (bran milk 1.08g / 200gr). The intervention was given for fourteen days. The data analysis used the paired T-test to test the subject before and afterthe treatment and the One-Way ANOVA test was used to test the difference between the groups. The research results indicated that  the treatment with bran milk there were no significant differences in cholesterol and triglyceride concentrations in the four groups. But when viewed from changes before and after the intervention showed that group 4 (bran milk 1.08g / 200gr) decreased cholesterol concentration and in group 3 (bran milk 0.54g / 200gr) a decrease in triglycerides was better than group 1 (control negative) and group 2 (positive control). It was concluded that the administration of bran milk can reduce the concentration of cholesterol and triglycerides in wistar diabetic white rats.Keywords : Bran milk, Cholesterol, Triglycerides, Rats, Diabetic

scholarly journals Antilipoxygenase and Anti-Inflammatory Activities of Streblus asper Leaf Extract on Xylene-Induced Ear Edema in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Kanathip Singsai ◽  
Pimchanok Charoongchit ◽  
Waritsara Chaikaew ◽  
Nirut Boonma ◽  
Pitsinee Fhanjaksai ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Animal Study ◽  
Leaf Extract ◽  
Diclofenac Sodium ◽  
Pharmaceutical Products ◽  
Control Group ◽  
Ear Edema ◽  
Anti Inflammatory ◽  
Streblus Asper

Streblus asper (SA) belonging to the Moraceae family is well known as a folk medicinal plant in Asian countries. This study aimed to investigate the antilipoxygenase activity and the anti-inflammatory effects of the SA leaf extract. An in vitro antilipoxygenase study was performed using a lipoxygenase assay, and the oxidation of linoleic acid into 13-hydroperoxy linoleic acid (HPODE) was detected with a UV spectrophotometer at a wavelength of 234 nm. In the animal study, twenty-five male ICR mice were induced as ear edema by topical xylene, and the ear thickness of the mice was measured. The lipoxygenase assay results showed that the IC50 values of diclofenac sodium and SA were 0.0015 and 37.96 μg/mL, respectively. In the animal study, mice that received diclofenac sodium exhibited significantly reduced ear edema induced by xylene from 30 min onward, while mice that received 250 mg/kg and 500 mg/kg SA exhibited significantly reduced ear edema compared with the control group 45 min after induction with xylene. These results suggested that the SA leaf extract had anti-inflammatory activity. However, further studies are required to evaluate these effects and the additional potential of the plant that might be beneficial for the development of pharmaceutical products that prevent and treat inflammation.

scholarly journals Effectivity of Holothuria scabra and Spirulina platensis extract combination as an Antiinflammatory Agent Measured by Carrageenan-induced Rat Paw Edema

2020 ◽  
Vol 25 (3) ◽  
pp. 103-109
Author(s):  
Renni Yuniati ◽  
Bambang Sulardiono
Keyword(s):  
Spirulina Platensis ◽  
Diclofenac Sodium ◽  
Positive Control ◽  
Control Group ◽  
Paw Edema ◽  
Holothuria Scabra ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Edema Inhibition ◽  
Inflammatory Effect

Sea cucumber, Holothuria scabra, can be found abundantly in Indonesian seas, which is also known to possess several medicinal properties. Spirulina platensis is another marine resources that has recently been extensively researched for its medicinal ability, such as anti-inflammatory effect. This study aims to evaluate the efficacy of H. scabra extract combined with S. platensis extract in reducing inflammation. This study uses male Wistar rats as the study animal. Inflammation was induced by injecting carrageenan solution into the mice paw. Combination of H. scabra and S. platensis extract with various combination ratio (1:1; 1:2; and 2:1) was applied to the mice paw. Diclofenac sodium was used as the standard control therapy. Edema inhibition rate and anti-inflammatory efficacy were measured by analyzing the edema size and calculating the edema difference. Combination of H. scabra and S. platensis with 1:1 ratio has the largest edema inhibition volume compared to the other treatments. H. scabra and S. platensis combination outperforms the positive diclofenac sodium control group in terms of edema inhibition. The highest anti-inflammatory effect is obtained in the combination of H. scabra and S. platensis with 1:2 ratio, however, the anti-inflammatory efficacy is not as potent as the positive control. The effectivity of Holothuria scabra and Spirulina platensis extract in reducing the edema might be caused by their ability to reduce the levels of several inflammatory markers, including IL-6, NO, MMP9, and COX-2. This result suggests that H. scabra and S. platensis combination has anti-inflammatory effect shown in mice paw edema model.

scholarly journals Antipyretic effect of key lime (Citrus aurantiifolium) leaf extract on white rats

2012 ◽  
Vol 10 (2) ◽  
pp. 35-39
Author(s):  
AMELIA KARTIKA WIDOWATI ◽  
NUR HAFIDHA HIKMAYANI ◽  
ETI PONCORINI PAMUNGKASARI
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antipyretic Effect ◽  
White Rats ◽  
Key Lime ◽  
One Way Anova

Widowati AK, Hikmayani NH, Pamungkasari EP. 2012. Antipyretic effect of key lime (Citrus aurantifolia) leaf extract on white rats. Biofarmasi 10: 35-39. The objective of this study was to evaluate the antipyretic effect of key lime (Citrus aurantifolia L.) leaf extract on white rats (Rattus norvegicus) induced fever by using DPT vaccine. Thirty male white rats with the body weight of ±200 grams and the age of 2-3 months old were used in this study. Those rats were grouped equally into five groups, consisted of a negative control group (2 mL aquabidest), a positive control group (acetaminophen 6.3 mg/100 g of body weight), the first dose of key lime leaf extract (1.26 mg/100 g of body weight), the second dose (2.52 mg/100 g of body weight) and the third dose (5.04 mg/100 g of body weight). The measurement of rat temperature was conducted before and 2 hours after getting DPT vaccine, and every 30 minutes posttreatment until 120 minutes. Data at the 120th minute were analyzed by using One-Way Anova test. The results of One-Way Anova test showed that there were significant differences (p<0.05) among treatment groups. The results of post-hoc test analysis showed that the significant differences (p<0.05) were found between a negative control group and four other groups. There was no significant difference (p>0.05) between a positive control group and three groups of key lime leaf extract. It concluded that the key lime leaf extract has an antipyretic effect on white male rats.

scholarly journals UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) Dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) PADA TIKUS JANTAN PUTIH

2019 ◽  
Vol 2 (1) ◽  
pp. 24-28
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Purpose: To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide. Method: This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg/kgMB, group 3 single roselle extract dose 130 mg/kgMB, group 4 single meniran leaf extract dose 200 mg/kgMB, group 5 combination of meniran leaf extract dose of 100 mg/kgMB and rosella extract dose of 65 mg/kgMB, group 6 combination of meniran leaf extract dose of 200 mg/kgMB and rosella extract dose of 130 mg/kgMB, and group 7 meniran leaf extract combination dose 400 mg/kgMB and rosella extract dose 195 mg/kgMB. Result: The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella gave a significant effect compared to the negative control group did not have a significant effect in reducing blood glucose levels. Conclusion: Single meniran extract and high-dose combination extract are more effective than glibenclamide.

UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) pada TIKUS JANTAN PUTIH SEBAGAI HEWAN PERCOBAAN

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Background : Diabetes mellitus (DM) is a chronic disease caused by the body's inability to produce the hormone insulin or due to ineffective use of insulin production. Diabetes has become a global problem, DM prevalence of 4.6%, estimated in 2000 amounted to 5.6 million. One of the plants that can reduce blood glucose levels is meniran and rosella which are found in Indonesia. Objective : To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide.Method : This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg / kgBB, group 3 single roselle extract dose 130 mg / kgBB, group 4 single meniran leaf extract dose 200 mg / kgBB, group 5 combination of meniran leaf extract dose of 100 mg / kgBB and rosella extract dose of 65 mg / kgBB, group 6 combination of meniran leaf extract dose of 200 mg / kgBB and rosella extract dose of 130 mg / kgBB, and group 7 meniran leaf extract combination dose 400 mg / kgBB and rosella extract dose 195 mg / kgBB. Result : The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella where p <0.05 gave a significant effect compared to the negative control group p> 0.05 did not have a significant effect in reducing blood glucose levels. Conclusion : Single meniran extract and high-dose combination extract are more effective than glibenclamide.

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