Comparison of Anti-inflammatory, Analgesic, Antipyretic Properties of Selected Red algae - Hypnea musciformis (Wulfen) J V Lamouroux., Gracilaria dura (C Agardh) J Agardh. and Kappaphycus alvarezii (Doty) Doty ex Silva

2020 ◽  
Vol 13 (5) ◽  
pp. 5110-5119
Author(s):  
Murugan K ◽  
Sumayya S s ◽  
Lubaina A S
Keyword(s):  
Acetic Acid ◽  
Red Algae ◽  
Kappaphycus Alvarezii ◽  
Dependent Manner ◽  
Hypnea Musciformis ◽  
Hot Plate ◽  
Writhing Test ◽  
Antipyretic Activity ◽  
Anti Inflammatory ◽  
Gracilaria Dura

Marine natural products from seaweeds have been the focus of research for novel products of pharmacological interest. Bioactivities of the phytochemicals derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other lifestyle disorders. In this context, the aim of the present study is to compare the anti-inflammatory, analgesic, antipyretic properties of the purified terpenoids from the selected red algae Hypnea musciformis, Gracilaria dura and Kappaphycus alvarezii. Initially, the acute toxicity of the purified terpenoid extract was analyzed and was greater than 4000 mg/kg bw. In vitro BSA denaturation assay revealed significant % inhibition as compared to control. In the carrageenan induced paw edema, the purified terpenoids extract of H. musciformis exhibited remarkable soothing activity with 68.8% percentage of inhibition, which was comparable with that of indomethacin. Meanwhile G. dura and K. alvarezii showed 53 and 50% of inhibition respectively. Analgesic activity was determined by hot plate and acetic acid induced writhing test. In the hot plate test, terpenoids extract of H. musciformis and G. dura significantly increased the hot-plate latency as compared to normal saline, which reflects their analgesic efficacy. In the writhing test, terpenoid extracts from H. musciformis, G. dura and K. alvarezii inhibited the writhing response induced by acetic acid in a concentration dependent manner that suggests its varied mode of inhibition of stretching episodes. Lastly, the antipyretic activity was analyzed. Terpenoid extracts from H. musciformis, G. dura and K. alvarezii revealed a significant (P < 0.01) antipyretic activity up to 180 min.  In summary, the study demonstrates the anti-inflammatory, analgesic and antipyretic effects of terpenoids extracts from H. musciformis, G. dura and K. alvarezii on experimental models, suggesting its therapeutic potential in the treatment of peripheral painful and inflammatory pathologies.

scholarly journals Enzymes associated with anti-inflammatory potentialities of purified terpenoid extracts from the selected sea weeds

2020 ◽  
Vol 11 (3) ◽  
pp. 497-505
Author(s):  
Sumayya S S ◽  
Lubaina A S ◽  
Murugan K
Keyword(s):  
Xanthine Oxidase ◽  
Red Algae ◽  
Inhibitory Activity ◽  
Kappaphycus Alvarezii ◽  
Immune Defense ◽  
Control Group ◽  
Dependent Manner ◽  
Hypnea Musciformis ◽  
Standard Drug ◽  
Anti Inflammatory

Introduction: Macrophages are phagocytic WBCs involved in the immune defense and will be activated during inflammatory disorders. The synthesis of cytokines and mediators, particularly nitric oxide (NO) is triggered by the macrophage activator. NO induces many biological events. Therefore, NO regulation is proven to be potential for exploring anti-inflammatory drugs. Aim: The anti-inflammatory action of the purified terpenoid extracts from red algae such as Hypnea musciformis, Gracilaria dura and Kappaphycus alvarezii on LPS induced RAW 264.7 macrophages on lipoxygenase, cyclooxygenase, hyaluronidase, xanthine oxidase and myeloperoxidase inhibitory effects were evaluated. Methods: The methanolic extract of the sea weeds were subjected to silica gel column chromatography and the fraction was further subjected to GC-MS analysis. Then, the potentiality of the purified terpenoid extracts to inhibit various inflammation causing enzymes such as COX, LOX, hyaluronidase, xanthineoxidase and myeloperoxidase were carried out. Findings: The terpenoid extracts reduced the enzyme activities in a dose dependent manner as compared to control group. The extracts inhibited xanthine oxidase activity effectively at 250 µg/ml i.e., a maximum inhibitory activity of 62.1% as compared to the standard drug, allopurinol. The extract significantly inhibited lipoxygenase activity, with highest inhibitory activity at 100 µg/ml. The nitric acid synthesis was reduced to 8.5 µM by Hypnea musciformis. Conclusion: The present study revealed that the purified terpenoid extracts from H. musciformis exhibited potent anti-inflammatory activities followed by G. dura and K. alvarezii via regulating the anti-inflammatory enzymes. These findings provide justification for the traditional use of the red algae in inflammatory conditions.

scholarly journals In vivo and in vitro pharmacological activities of Tacca integrifolia rhizome and investigation of possible lead compounds against breast cancer through in silico approaches

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Shahriar Ahmed ◽  
Ahmed Rakib ◽  
Md. Ashiqul Islam ◽  
Bibi Humayra Khanam ◽  
Farhana Binta Faiz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Acetic Acid ◽  
Molecular Docking ◽  
Analgesic Activity ◽  
In Silico ◽  
Antineoplastic Activity ◽  
Paw Edema ◽  
Writhing Test ◽  
Antipyretic Activity ◽  
Anti Inflammatory

Abstract Background The study was conducted to evaluate the analgesic, anti-inflammatory, antipyretic properties of ethanolic extract of Tacca integrifolia rhizome (EETI) in mice and cytotoxic effects in brine shrimp nauplii followed by a PASS prediction study for some isolated compounds of T. integrifolia. Additionally, this experiment included the in silico molecular docking and ADME/T property analyses of some phytochemicals. Methods Formalin- induced paw licking test and acetic acid-induced writhing test for analgesic activity, carrageenan-induced paw edema test for anti-inflammatory potential and Brewer’s yeast-induced pyrexia test for antipyretic activity were applied. Antinociceptive and antineoplastic activity for breast cancer were revealed with PASS program. Schrodinger suite 2015 was used to evaluate the binding interaction and ADME/T properties of selected phytoconstituents with estrogen receptor alpha. Results In formalin-induced paw licking test, EETI at the doses of 200 and 400 mg/kg BW showed highly significant inhibition of writhing in both neurogenic and inflammatory phases. While EETI also exhibited highly significant, compared to control, writhing inhibition for both the doses in acetic acid-induced writhing test. Moderate anti-inflammatory effect at a dose of 400 mg/kg BW was noticed in paw-edema test. It also showed 77.51% of maximum antipyretic effect which was significantly effective compared to standard drug paracetamol (150 mg/kg) in Brewer’s yeast-induced pyrexia test. The EETI showed potential cytotoxic activity with LC50 value of 114.46 μg/mL. The PASS prediction revealed the potential antinociceptive and antineoplastic activity of target compounds. The compounds betulinic acid, quercetin-3-α-arabinoside, catsanogenin were found to be effective in molecular docking study. Conclusion It is evident that the EETI possesses highly significant analgesic activity with remarkable anti-inflammatory and antipyretic activity. The phytoconstituents have potential antinociceptive and antineoplastic (breast cancer) activity. Graphical abstract

scholarly journals INVESTIGATING THE ROLE OF NF-κB, COX-1, COX-2, COMT, IL-10, IL-6 AND TNF-α IN MODULATING ANTI-NOCICEPTIVE ACTIVITY OF METHANOLIC EXTRACT OF ENTADA PHASEOLOIDES

2020 ◽  
pp. 172-179
Author(s):  
CHANDANA CHOUDHURY BARUA ◽  
S. M. ABDUL AZIZ BARBHUIYA ◽  
LIPIKA BURAGOHAIN ◽  
AJAY KAKATI ◽  
ACHEENTA GOHAIN BARUA
Keyword(s):  
Methanolic Extract ◽  
Water Immersion ◽  
Hot Water ◽  
Dependent Manner ◽  
Hot Plate ◽  
Writhing Test ◽  
Tnf Α ◽  
Entada Phaseoloides ◽  
Anti Inflammatory ◽  
Cox 1

Objective: To investigate the analgesic activity of methanolic extract of Entada phaseoloides (MEEP) along with its molecular mechanistic pathway. Methods: Swiss albino female mice and Wistar rats of either sex were administered orally with MEEP extracts (100, 200 and 400 mg/kg) and pentazocine, tramadol and diclofenac sodium, as standard drugs. Following administration, anti-nociceptive activity was evaluated using an acetic acid-induced writhing test, Eddy’s hot plate, and hot water immersion test. Serum was collected for molecular expression of various proteins and genes using Reverse Transcriptase PCR and Western Blotting. Results: Acetic acid writhing test, a frequently used method to assess peripheral analgesic activity, revealed that MEEP reduced peripherally induced pain in a dose-dependent manner. Likewise, Eddy’s hot plate and hot water immersion methods, often implicated for testing central analgesic activities, showed that MEEP is bestowed with the capability to counteract analgesia in a dose and time-dependent manner. Pro-inflammatory cytokines and factors like COX-2, IL-6, TNF-α, and NF-κB that cause inflammatory responses and pain were significantly reduced, suggesting its analgesic and anti-inflammatory potential. This analgesic and the anti-inflammatory role played by MEEP is also supported by the up-regulation of anti-inflammatory cytokine IL-10 and COMT and COX-1 enzyme demonstrated no significant difference between the groups. Conclusion: The study revealed the weak peripheral and potent central analgesic property MEEP by modulating pro-inflammatory and anti-inflammatory pathways.

scholarly journals Assessment of Analgesic, Anti-Inflammatory And Anti-Pyretic Activities of Malva Neglecta Wallr. In Animal Model

2021 ◽  
Vol 50 (3) ◽  
pp. 577-583
Author(s):  
Rahma Hamayun ◽  
Muhammad Shahid Iqbal ◽  
Muhammad Imran Qadir
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Aqueous Ethanol ◽  
Mice Model ◽  
Hot Plate ◽  
Standard Drug ◽  
Antipyretic Activity ◽  
Ethanolic Extracts ◽  
Anti Inflammatory ◽  
Ethanol Extracts

Analgesic, anti-inflammatory and anti-pyretic activities of n-hexane and aqueous ethanolic extracts of Malva neglecta in Swiss albino mice was evaluated. For each activity, the mice were divided into 4 groups: Group 1 served as control, Groups 2 and 3 were given n-hexane and aqueous ethanol extracts, respectively whereas Group 4 was treated with the standard drug. Analgesic activity was evaluated against acetic acid induced writhing, Eddy's hot plate method, and Formalin induced paw licking. Anti-inflammatory activity was evaluated by carrageenan-induced paw edema. The extracts were also examined for their anti-pyretic activities against yeast-induced pyrexia. Results showed that the n-hexane (p < 0.05) and aqueous ethanolic (p < 0.005) extracts of M. neglecta exhibited analgesic activity by reducing acetic acid-induced writhing, mean reaction time on hot plate model and formalin-induced paw licking in mice as compared to the control. The n-hexane extract (p < 0.05) as well as aqueous ethanolic (p < 0.005) extracts produced significant antiinflammatory activity as compared to the control. Both the n-hexane and aqueous ethanolic extracts revealed significant antipyretic activity (p < 0.005 and p < 0.05, respectively) in mice model. The results of the present study demonstrated that the n-hexane and aqueous ethanol extracts of M. neglecta possess analgesic, antiinflammatory and anti-pyretic activities. Bangladesh J. Bot. 50(3): 577-583, 2021 (September)

scholarly journals INVESTIGATION OF ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS OF AQUEOUS EXTRACTS OF ARTEMISIA AFRA IN WISTAR RATS

2018 ◽  
Vol 11 (12) ◽  
pp. 190 ◽  
Author(s):  
Mavuto Gondwe ◽  
Anda Mpalala ◽  
Lusanda Zongo ◽  
David Kamadyaapa ◽  
Eugene Ndebia ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Biological Effects ◽  
Control Group ◽  
Paw Edema ◽  
Hot Plate ◽  
Traditional Practice ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Antinociceptive Effects ◽  
Anti Inflammatory

Objective: The objective of the study was to evaluate the anti-inflammatory and antinociceptive effects of Artemisia afra.Methods: Animals were randomly divided into five groups of six animals each and administered with normal saline (2 ml/kg), indomethacin (10 mg/ kg), and A. afra at doses of 100, 200, and 400 mg/kg, respectively. For the anti-inflammatory activity, carrageenan-induced paw edema was used while the hot plate and acetic acid induced-writhing tests were used to assess the antinociceptive activity.Results: Pretreatment with A. afra at a dose of 100 mg/kg did not show any significant biological effects (p>0.05) for any of the three tests, when compared against saline-treated control group. At a dose of 200 mg/kg, A. afra demonstrated significant effects (p<0.01), during the 5th h reducing carrageenan-induced paw edema by 12%. The highest dose (400 mg/kg) of A. afra demonstrated more potent effects by decreasing the carrageenan-induced paw swelling (p<0.001–0.05) during the 3rd, 4th, and 5th h, by up to 38% when compared against saline-treated control group. Both the 200 and 400 mg/kg, A. afra doses achieved a significant increase (p<0.05) in reaction time in the hot plate test. In the acetic acid-induced writhing test, pretreatment with A. afra (400 mg/kg) significantly reduced pain by 39% (p<0.01) by comparison with the saline control.Conclusion: Experimental data demonstrated that aqueous extract of A. afra possesses anti-inflammatory and antinociceptive properties in experimental acute inflammation and pain. These findings support the usage of A. afra in managing inflammation and pain in traditional practice.

In vivo analgesic, antipyretic and anti-inflammatory potential of leaf extracts and fractions of Eria javanica

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
N. M. Mahmudul Alam Bhuiya ◽  
Mahmud Hasan ◽  
Zobaer Al Mahmud ◽  
Nazmul Qais ◽  
Mohammad Shah Hafez Kabir ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Leaf Extracts ◽  
Inhibition Study ◽  
Writhing Test ◽  
Antipyretic Activity ◽  
Activity Test ◽  
Anti Inflammatory

AbstractBackgroundThe objective of the study was to evaluate the antinociceptive, antipyretic and anti-inflammatory activities of ethanolic extract, methanolic extract andMethodsThe anti-nociceptive potentials of the extracts were studied using the acetic acid-induced writhing test in mice and the antipyretic activity was investigated using yeast-induced pyrexia in rats. Anti-inflammatory activity test was done on rats at a dose by using carrageenan-induced paw edema test.ResultsIn acetic acid-induced writhing inhibition study in Swiss albino mice, the crude methanolic extract at 200 mg/kg and 400 mg/kg doses and theConclusionsThis study thereby indicates that leaves of

scholarly journals Evaluation of antinociceptive and antipyretic effect of Pupalia lappacea Juss

2013 ◽  
Vol 2 (2) ◽  
pp. 23-28 ◽  
Author(s):  
Vajrala Neeharika ◽  
Humaira Fatima ◽  
Bommineni Madhava Reddy
Keyword(s):  
Acetic Acid ◽  
Antinociceptive Effect ◽  
Ethanolic Extract ◽  
Pharmaceutical Journal ◽  
Dependent Manner ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Antipyretic Activity ◽  
Cns Depressant ◽  
Dose Dependent

Pupalia lappacea Juss (Family: Amaranthaceae) is claimed to be useful in treatment of bone fracture, wounds, boils, cough, toothache, fever and malaria. The study was aimed to evaluate the 80% aqueous ethanolic extract of aerial parts of P. lappacea for antinociceptive and antipyretic activities to verify the traditional claim. The extract was orally administered at doses of 200, 400 and 600 mg/kg. The extract has significantly (P<0.01) reduced the nociception induced by acetic acid. The effect produced was in dose dependent manner. The antinociceptive effect was not reversed by pretreatment with naloxone in acetic acid induced writhing test. In hot plate method, the extract has significantly increased the latency time of jump. The naloxone has partially antagonised the antinociception of extract in hot plate test indicating P. lappacea has morphinomimetic properties. In the study of the CNS-depressant effects, the extract was found to produce significant reduction in head pokes and locomotion in mice by using hole board and locomotor activity test respectively. The extract has significantly reduced the rectal temperature in yeast induced pyrexia in rats at 600 mg/kg. The activity produced by extract was in dose dependent manner. Phytochemical investigation of ethanolic extract of P. lappacea revealed the presence of steroids and/or triterpenoids, flavonoids and phenolic compounds which may be responsible for antinociceptive and antipyretic activity of P. lappacea.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13193 International Current Pharmaceutical Journal 2013, 2(2): 23-28

scholarly journals Anti-Inflammatory and Analgesic Evaluation of Parinari curatellifolia Methanol Leaf Extract on Albino Rats

2021 ◽  
pp. 9-18
Author(s):  
I. M. Fakai ◽  
A. Abdulhamid ◽  
Alhassan Yunusa Dada
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Leaf Extract ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Hot Plate ◽  
Writhing Test ◽  
Egg Albumin ◽  
Parinari Curatellifolia ◽  
Anti Inflammatory

Aim: This research was designed to evaluate the anti-inflammatory and analgesic activities of Parinari curatellifolia methanol leaf extract in albino rats. Methodology: Phytochemical screening was carried out using standard methods. Anti-inflammatory activity of the extract was done using egg albumin and formalin induced hind paw edema model. Analgesic effect was evaluated using hot plate induced pain and acetic acid induced writhing test. For each model twenty (20) rats were used, divided into five (5) groups of four (4) rats each. Results: Parinari curatellifolia revealed the presence of alkaloids, flavonoids, tannins and phenols while steroids, anthraquinone, terpenoids and glycoside were not detected. For the egg albumin induced inflammation, the group treated with the standard drug (indomethacin) and the group that received the highest dose of the extract were significantly lower (P<0.05) than all the other groups with percentage inhibitions at 25.56% and 24.44% respectively there was no significant difference (P>0.05). For the formalin induced anti-inflammatory activity, at the 1st hour, the normal control group had its paw volume significantly different (P<0.05) from the treated groups. This trend was observed at the 2nd, 3rd and 4th hour. The hot plate method results revealed significant increased (P<0.05) in the analgesic activity of PCMLE at 400mg/kg body weight and the drug treated group when the control was compared with the treated groups with percentage inhibition of 34.32% and 52.94% respectively. The acetic acid induced writhing test revealed that the extract at the three doses of 100, 200 and 400 mg/kg body weight, showed a significant (P<0.05) percentage inhibition of 32.31%, 36.92% and 47.69%, respectively compared to negative control. Conclusion: This justifies the use of Parinari curatellifolia locally in the management of pain and inflammation.

scholarly journals COMPARISON OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF PURIFIED ANTHOCYANIN FROM OSBECKIA ASPERA (L.) BLUME AND OSBECKIA RETICULATA BEDD. USING ANIMAL MODELS

2019 ◽  
Vol 12 (1) ◽  
pp. 106
Author(s):  
Bosco Lawarence ◽  
Murugan K
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Callus Formation ◽  
Naphthalene Acetic Acid ◽  
Immersion Method ◽  
Hot Plate ◽  
Writhing Test ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Objective: The objective of the present work is to isolate, purify, and fractionate anthocyanin from selected Osbeckia species and also to compare the analgesic and anti-inflammatory potentiality using animal models.Methods: Methodologies include extraction of anthocyanin from the in vitro callus culture of Osbeckia aspera and Osbeckia reticulata, purification using amberlite column chromatography, and fractionation by liquid chromatography-tandem mass spectrometry. The analgesic activity was determined by tail immersion method, analgesy meter, hot plate, and acetic acid-induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced paw inflammation in mice.Results: Anthocyanin-producing callus cultures were established in MS medium fortified with various combinations of phytohormones and sucrose. Optimal callus formation in O. aspera was initiated on cultures containing 0.5 mg/L of 2, 4-D, and 0.5 mg/L 6-benzylaminopurine (BA). In O. reticulata callus was initiated in the presence of 1.2 mg/L BA and 1.4 mg/L naphthalene acetic acid. The same hormonal combination on extended treatments turned the white friable callus into red compact callus. Anthocyanins obtained from Osbeckia species were purified and fractionated containing malvidin-3-diglucoside, delphinidin, cyanidin aglycone, and peonidin. Purified anthocyanin of O. aspera at the concentrations 50, 100, 150, and 200 mg/kg after 120 min exhibited significant analgesic activity by tail immersion method, in comparison to O. reticulata. However, with hot plate method, anthocyanin of O. reticulata produced a significant analgesic activity even at lower doses (50 and 100 mg/kg) after 120 min. However, in writhing test, theepurified anthocyanin of O. aspera significantly stopped the number of writhes at a dose of 200 mg/kg and also by the anthocyanin of O. reticulata. In the evaluation of anti-inflammatory effect using plethysmometer, anthocyanin at doses of 100,150 and 200 mg/kg started producing anti-inflammatory effect after 30 min, which lasted until 120 min.Conclusion: It is concluded from the present study that the purified anthocyanin of Osbeckia possesses potent analgesic and anti-inflammatory activities.

scholarly journals ANALGESIC, ANTI-INFLAMMATORY, ANTIPYRETIC EVALUATION OF ETHANOLIC EXTRACT OF LEAF OF POLYALTHIA LONGIFOLIA

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Ramjith.U. S ◽  
Faby Salim ◽  
Ashamol.K. S ◽  
Cyril Mathews Jacob
Keyword(s):  
Acetic Acid ◽  
Ethanolic Extract ◽  
Percentage Increase ◽  
Hot Plate ◽  
Plate Method ◽  
Percentage Decrease ◽  
Antipyretic Activity ◽  
Ethanolic Extracts ◽  
Anti Inflammatory ◽  
Polyalthia Longifolia

Polyalthia longifolia leaves ethanolic extracts produced significant analgesic activity in both Hot plate and acetic acid induced writhing models in mice. In hot plate method percentage increase in reaction time was determined where as in acetic acid induced writhing model percentage decrease in writhings was determined. From the results obtained it can be concluded that Polyalthia longifolia leaves has analgesic, anti inflammatory and antipyretic activity. Keywords: Analgesic, Anti-inflammatory, Antipyretic, Hot plate method, Acetic acid induced writhing model.

Sign in / Sign up

Export Citation Format

Share Document