Effects of Cyanidin-3-Glucoside Derived from Black Rice (Oryza Sativa L.) and Enzymatically Acylated Cyanidin-3-Glucoside Lauryl Ester on Intestinal Microorganisms In Vitro

2021 ◽  
Vol 20 (1) ◽  
pp. 159-168
Author(s):  
Yuan Jin ◽  
Zuoyong Zhang ◽  
Hanju Sun ◽  
Muwen Wang ◽  
Shuyun Liu ◽  
...  
Keyword(s):  
Lauric Acid ◽  
High Throughput Sequencing ◽  
Oryza Sativa L ◽  
Intestinal Flora ◽  
Inhibitory Effect ◽  
Black Rice ◽  
Phenyllactic Acid ◽  
Proliferative Effect ◽  
Enzymatic Acylation

In this paper, cyanidin-3-glucoside was isolated and purified from black rice, then enzymatic acylation with lauric acid to obtain cyanidin-3-glucoside lauryl ester. The structure of cyanidin-3-glucoside lauryl ester was characterized by liquid chromatography electrospray ionization tandem mass spectrometry and Fourier transform infrared spectroscopy. Then the potential proliferative effect on probiotics and inhibitory effect on harmful bacteria of cyanidin-3-glucoside and cyanidin-3-glucoside lauryl ester were studied in vitro. The effects of cyanidin-3-glucoside and cyanidin-3-glucoside lauryl ester on the composition of human fecal intestinal flora and its metabolic pathway were also analyzed through 16S ribosomal ribonucleic acid high-throughput sequencing and gas chromatography-mass spectroscopy, respectively. The results indicated that cyanidin-3-glucoside lauryl ester was better than cyanidin-3-glucoside in promoting the growth of B. adolescentis, B. infantis, L. thermophiles, and L. acidophilus as well as inhibiting the growth of S. dysenteriae, Y. enterocolitica, S. enteritidis, and S. typhi. Additionally, the proliferative effect of cyanidin-3-glucoside lauryl ester was significantly improved in a lower media pH due to the intestinal microbial metabolism to produce more organic acids, such as propionic acid, phenyllactic acid, and lauric acid. The study will provide a theoretical basis for the application of cyanidin-3-glucoside lauryl ester in the intestinal health.

scholarly journals Co-Microencapsulated Black Rice Anthocyanins and Lactic Acid Bacteria: Evidence on Powders Profile and In Vitro Digestion

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2579
Author(s):  
Carmen-Alina Bolea ◽  
Mihaela Cotârleț ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
Nicoleta Stănciuc
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Oryza Sativa L ◽  
Milk Proteins ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Hot Water ◽  
Raw Material ◽  
Black Rice

Two multi-functional powders, in terms of anthocyanins from black rice (Oryza sativa L.) and lactic acid bacteria (Lactobacillus paracasei, L. casei 431®) were obtained through co-microencapsulation into a biopolymer matrix composed of milk proteins and inulin. Two extracts were obtained using black rice flour as a raw material and hot water and ethanol as solvents. Both powders (called P1 for aqueous extract and P2 for ethanolic extract) proved to be rich sources of valuable bioactives, with microencapsulation efficiency up to 80%, both for anthocyanins and lactic acid bacteria. A higher content of anthocyanins was found in P1, of 102.91 ± 1.83 mg cyanindin-3-O-glucoside (C3G)/g dry weight (DW) when compared with only 27.60 ± 17.36 mg C3G/g DW in P2. The morphological analysis revealed the presence of large, thin, and fragile structures, with different sizes. A different pattern of gastric digestion was observed, with a highly protective effect of the matrix in P1 and a maximum decrease in anthocyanins of approximatively 44% in P2. In intestinal juice, the anthocyanins decreased significantly in P2, reaching a maximum of 97% at the end of digestion; whereas in P1, more than 45% from the initial anthocyanins content remained in the microparticles. Overall, the short-term storage stability test revealed a release of bioactive from P2 and a decrease in P1. The viable cells of lactic acid bacteria after 21 days of storage reached 7 log colony forming units (CFU)/g DW.

Sesamin Modulation of Intestinal Microflora in Rats on High-Fat Diet

2020 ◽  
Vol 19 (3) ◽  
pp. 312-316
Author(s):  
Pan Liu ◽  
Yu Deng ◽  
Mengxi Wang ◽  
Lingyan Kong ◽  
Na Xu ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Intestinal Microflora ◽  
High Throughput Sequencing ◽  
Intestinal Flora ◽  
Short Chain Fatty Acids ◽  
Sequencing Analysis ◽  
Anaerobic Culture ◽  
High Fat ◽  
E Coli

The present study aimed to investigate the effects of sesamin on intestinal flora in rats on high-fat diet by in vitro animal fecal anaerobic culture system. Results showed that treatment with sesamin increased the abundance of Lactobacillus acidophilus and inhibited the growth of E. coli. High throughput sequencing analysis showed that treatment with sesamin increased the abundance of probiotics such as Lactobacillus and Flavonifractor, and decreased the abundance of harmful bacteria such as Bacteroides, Enterococcus, Clostridium_XlVa, and Parabacteroides. Moreover, treatment with sesamin increased the concentration of total short chain fatty acids (acetic acid and isovaleric acid). In conclusion, sesamin intervention improved the composition and abundance of intestinal microorganisms of high fat diet rats, which would be beneficial to intestinal health.

ACE Inhibitory, Antitumor and Antioxidant Activities of Submerged Culture Materials of Three Medicinal Mushrooms

2011 ◽  
Vol 145 ◽  
pp. 179-183 ◽  
Author(s):  
Xiu Jun Gao ◽  
Pei Sheng Yan ◽  
Jian Bing Wang ◽  
Jia Jia Yu
Keyword(s):  
Inhibitory Activity ◽  
Natural Compound ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Ace Inhibitory Activity ◽  
Medicinal Mushrooms ◽  
Proliferative Effect ◽  
Ace Inhibitory ◽  
Lyophyllum Decastes

Mushrooms have been highly valued as tonic foods and herb remedies since ancient times, and have been increasingly concerned because of their biological and pharmacological activities recognized through recent scientific research. Antitumor, antioxidant and ACE inhibitory activities in vitro of liquid fermentation materials from Ramaria botrytoides, Lyophyllum decastes and Mycoleptodonoides aitchisonii were investigated in this study. Water extracts from fermented mycelia and supernatant materials were used to measure their antitumor effect by MTT assay, antioxidant capacity by kit, and ACE inhibitory activity by RP-HPLC. Results showed that mycelia extracts from Ramaria botrytoides has significantly higher anti-proliferative effect on human HCC cell line SMMC7721 (IC50=0.284±0.005mg/ml, p<0.001). Total antioxidant activity of mycelia extracts of Ramaria botrytoides (0.711±0.053U/mg), inhibitory effect of mycelia extracts of Mycoleptodonoidesaitchisonii on hydroxyl radical (86.153±1.763U/mg) and superoxide anion radical (7.363±0.280 U/g) were higher than others significantly (p<0.001). ACE inhibitory activity of mycelia extracts of Lyophyllum decastes (IC50=1.637±0.057mg/ml) was significantly higher than others (p<0.001). Results demonstrated that liquid fermented mycelium of Ramaria botrytoides was potent source of natural compound with antitumor and antioxidant potential. Mycelium of Lyophyllum decastes was potent source of natural compound with ACE inhibitory activity.

scholarly journals Metabolism and prebiotics activity of anthocyanins from black rice (Oryza sativa L.) in vitro

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0195754 ◽  
Author(s):  
Yongsheng Zhu ◽  
Hanju Sun ◽  
Shudong He ◽  
Qiuyan Lou ◽  
Min Yu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Black Rice

scholarly journals The Influence of Caerulomycin A on the Intestinal Microbiota in SD Rats

Marine Drugs ◽  
2020 ◽  
Vol 18 (5) ◽  
pp. 277
Author(s):  
Hongwei Zhang ◽  
Mengmeng Lan ◽  
Guodong Cui ◽  
Weiming Zhu
Keyword(s):  
High Throughput Sequencing ◽  
Intestinal Flora ◽  
Hypervariable Region ◽  
Sprague Dawley ◽  
16S Rdna Gene ◽  
Sd Rats ◽  
Anti Tumor Activity ◽  
Ring Core

Caerulomycin A (CRM A) is the first example of natural caerulomycins with a 2,2′-bipyridyl ring core and 6-aldoxime functional group from Streptomyces caeruleus and recently from marine-derived Actinoalloteichus cyanogriseus WH1-2216-6. Our previous study revealed that CRM A showed anti-tumor activity against human colorectal cancer (CRC) both in vitro and in vivo. Because some intestinal flora can affect the occurrence and development of CRC, the influence of CRM A on the intestinal flora is worthy of study in Sprague–Dawley (SD) rats. The high throughput sequencing of the V3-V4 hypervariable region in bacterial 16S rDNA gene results showed that the CRM A affected the diversity of intestinal flora of the SD rats treated with CRM A for 2, 3 and 4 weeks. Further analysis indicated that the abundance of genera Prevotella_1, Prevotellaceae_UCG-001, and Lactobacillus were increased while the that of genera Alloprevotella and Ruminiclostridium_1 were decreased. For the CRC related intestinal flora, the abundance of genera Bacteroides, Fusobacterium, Enterococcus, Escherichia-Shigella, Klebsiella, Streptococcus, Ruminococcus_2, and Peptococcus of SD rats treated with CRM A were decreased, while that of abundance of genera Bifidobacterium, Lactobacillus, Faecalibacterium, Blautia, Oscillibacter, and Clostridium were increased. The results indicated that CRM A could influence the intestinal flora by inhibiting some species of harmful flora and improving the beneficial bacteria in intestinal flora in the SD rats. The results may provide a new idea for revealing the mechanism of the anti-CRC activity of CRM A.

scholarly journals Inhibitory Effect of Oat Bran Ethanol Extract on Survival and Gemcitabine Resistance of Pancreatic Cancer Cells

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3829 ◽  
Author(s):  
Myoungjae Kim ◽  
Jeong-Geon Mun ◽  
Hyun Jin Lee ◽  
So-Ri Son ◽  
Mi-Ja Lee ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Synergistic Effect ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Proliferative Effect ◽  
Pancreatic Cancer Cells ◽  
Pancreatic Cells ◽  
Oat Bran ◽  
Pyrimidine Analogue

Pancreatic cancer (PC) is one of the most aggressive malignancies in the world. Gemcitabine (Gem), a nucleoside pyrimidine analogue, is a first-line chemotherapeutic drug for PC, but the tumor response rate of Gem is very low and resistance to Gem has emerged as a major problem in the treatment of PC. Oat bran, used as animal and human food, has been found to be beneficial to health. In this study, effects of oat bran ethanol extract (OBE) on PC cells and Gem-resistant PC cells were investigated in vitro. OBE decreased cell survival and colony forming ability of PC cells, without any cytotoxicity on the normal pancreatic cells. Flow cytometry analysis and TUNEL assay showed that the OBE reduced G1/S phase transition and induced death in PC cells through AMPK activation and downregulation of JNK. Additionally, OBE could overcome Gem resistance through reduction in RRM1/2 expression and showed synergistic effect by combinatorial treatment with Gem on Gem-resistant PC cells. Additionally, LC-MS data showed that avenacoside A was a component of OBE. Thus, this study elucidated the anti-proliferative effect of OBE and synergistic effect of OBE with Gem on PC cells and Gem-resistant cells.

Role of Calabrian Black Rice in Metabolic Syndrome: In vitro Evaluation of Oryza sativa L. Indica Biological Properties

2018 ◽  
Vol 14 (2) ◽  
pp. 121-127 ◽  
Author(s):  
Mariarosa Ruffo ◽  
Ortensia Ilaria Parisi ◽  
Luca Scrivano ◽  
Donatella Restuccia ◽  
Fabio Amone ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Biological Properties ◽  
Black Rice ◽  
In Vitro Evaluation
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