scholarly journals Uji Aktivitas Analgetik Ekstrak Etanol Daun Inggu (Ruta angustifolia [l.] Pers) Pada Tikus Putih Jantan

2019 ◽  
Vol 1 (2) ◽  
pp. 57-69
Author(s):  
Yane Dila Keswara ◽  
Sri Rejeki Handayani
Keyword(s):  
Mefenamic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Negative Control ◽  
Test Method ◽  
Narcotic Analgesic ◽  
Tail Flick ◽  
Daily Routine ◽  
White Rats ◽  
Pressure Test

Pain is an uncomfortable feeling caused by strong or destructive stimulation, if unchecked can affect daily routine. This study aims to determine the analgesic effect and dosage of the leaf extract ethanol, as well as to determine the effectiveness of the leaf extract ethanol as a narcotic analgesic by the Tail Flick method or as a non-narcotic analgesic by the Paw Pressure Test Randall Selitto method. Extraction was done by maceration of leaf powder with a solvent 96% ethanol. 25 male Wistar white rats were divided into 5 groups, positive group mefenamic acid 9 mg /200 g BW for the Paw Pressure Test Randall Selitto Method and tramadol 0.9 mg /200 g BW for the Tail Flick Method, negative control CMC Na 0,5%, ethanol extract leaves inggu leaves a dose of 10 mg /200 g BW, 20 mg /200 g BW and 40 mg /200 g BW. The data obtained were analyzed by ANOVA test, then LSD test was used to determine differences between groups. The results showed extract dosage of 10 mg /200 g BW, 20 mg /200 g BW, 40 mg /200 g BW had analgesic effect. Extract dosage of 20 mg /200 g BW and 40 mg /200 g BW in the Randall selitto Paw Pressure Test method is equivalent to mefenamic acid, extract dose 10 mg /200 g BW, 20 mg /200 g BW, and 40 mg /200 g BW the Tail Flick method is equivalent to Tramadol. Ethanol leaf extract is more effective as a narcotic analgesic by the Tail Flick method.

scholarly journals Preliminary assessment of the anti-inflammatory and analgesic effects of methanol leaf extract of Cussonia barteri (Araliaceae) in rodents

2019 ◽  
Vol 65 (3) ◽  
pp. 22-31
Author(s):  
Ighodaro Igbe ◽  
Osaze Edosuyi ◽  
Agbonlahor Okhuarobo ◽  
Adarki Pongri ◽  
Nkechi Maduako ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Tail Flick ◽  
Hot Plate ◽  
Analgesic Effects ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Ear Oedema

Summary Introduction: Potato (Solanum tuberosum L.) is an important vegetable crop in Syria. Potato tuber moth Cussonia barteri is a small tree that grows in the sub-Saharan part of Africa. Various parts of the plant are used for the treatment of a variety of ailments in ethno-medicine. Objective: To evaluate the anti-inflammatory and analgesic effect of the methanol leaf extract of Cussonia barteri. Material and methods: The leaves were air-dried, powdered and repeatedly extracted with methanol using a Soxhlet apparatus. The resulting methanol extract (100, 200 and 400 mg/kg) was evaluated for anti-inflammatory activity using carrageenan-induced paw oedema, xylene-induced ear oedema and formalin-induced arthritis tests. Analgesic effect was evaluated using acetic acid-induced mouse writhing, hot plate and tail flick tests. Results: All doses of the extract significantly (p<0.05) reduced carrageenan-induced paw oedema, however the 400 mg/kg dose gave a sustained effect. The extract significantly inhibited xylene induced ear oedema at all doses. There were no significant (p>0.05) reductions in paw swellings due to formalin. In the acetic acid induced writhing test, the extract significantly (p<0.05) decreased writhing at 400 mg/kg only. Reaction times were not significantly different from the control in the hot plate and tail flick tests. Conclusion: This study has shown that the methanol extract possesses acute anti-inflammatory and peripherally mediated analgesic effects.

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

scholarly journals Uji Efek Analgesik Ekstrak Daun Jarak Pagar (Jatropha Curcas L.) terhadap Tikus Wistar (Rattus Norvegicus)

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Oras B.M. Yensenem ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Jatropha Curcas ◽  
Rattus Norvegicus ◽  
Analgesic Effect ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Control Group ◽  
Tropical Country ◽  
Mean Values ◽  
Jatropha Curcas L

Abstract: Indonesia is a tropical country with the second largest biodiversity in the world after Brazil. Jatropha curcas (Jatropha curcas L.) was believed as a pain reliever (analgesic). This study was aimed to prove the analgesic effect of Jatropha curcas leaf extract on Wistar rats (Rattus norvegicus). This was an experimental study using hot stimulus method in the form of temperature of 65°C. Subjects were 15 male Wistar rats divided into 5 groups. Group 1, the negative control group; group 2, the positive control group given paracetamol 40 mg/kgBW; and 3 treatment groups (groups 3, 4, and 5) given Jatropha curcas extract in several doses (300 mg/kgBW, 600 mg/kgBW, and 1200mg/kgBW). Observation of Wistar rat respons (licking and jumping) was performed for 1 minute at minutes -30, 60, 90, and 120 after treatment. The results showed that the mean values of response against heat decreased in accordance to time and reached the lowest values at minute 120, except group 5 (the extract dose of 1200mg/kg BW) which reached the lowest value at minute 90. Overall, the lowest mean value was found in group 4 (the extract dose of 600mg/kg BW). Conclusion: Jatropha curcas (Jatropha curcas L.) leaf extract had an analgesic effect on Wistar rats (Rattus norvegicus).Keywords: Jatropha curcas L., Rattus norvegicus, analgesic Abstrak: Indonesia merupakan negara beriklim tropis dengan keaneka ragaman hayati terbesar kedua di dunia setelah Brazil. Penelitian ini bertujuan untuk mengetahui efek analgesik pemberian ekstrak daun jarak pagar (Jatropha curcas L.) pada tikus Wistar (Rattus norvegicus). Jenis penelitian ini ialah eksperimental dengan mengguna-kan metode rangsang panas berupa suhu 65°C. Subjek penelitian mengunakan hewan uji berupa tikus Wistar (Rattus norvegicus) jantan sebanyak 15 ekor yang dibagi menjadi 5 kelompok, yaitu: Kelompok kontrol negatif; kelompok kontrol positif yang diberikan parasetamol 40 mg/kgBB; dan 3 kelompok perlakuan yang diberikan ekstrak jarak pagar (Jatropha curcas L.), dengan dosis 300 mg/kgBB, 600 mg/kgBB, dan 1200mg/kgBB. Pengamatan terhadap respon tikus Wistar berupa jilat dan lompat dilakukan selama 1 menit pada menit ke-30, 60, 90, dan 120 setelah pemberian ekstrak jarak pagar. Hasil penelitian mendapatkan bahwa terdapat penurunan nilai rerata respons terhadap panas yang sesuai dengan perlangsungan waktu kecuali pada kelompok 5 (dosis ekstrak daun jarak pagar 1200mg/kg BB) yang mencapai nilai terendah pada menit ke-90. Secara keseluruhan, nilai rerata respons yang terendah didapatkan pada kelompok 4 (dosis ekstrak daun jarak pagar 600mg/kg BB) pada menit ke-120. Simpulan: Ekstrak daun jarak pagar (Jatropha curcas L.) memiliki efek analgesik terhadap tikus Wistar (Rattus norvegicus)Kata kunci: Jatropha curcas L., Rattus norvegicus, analgesic

scholarly journals Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica)  is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

scholarly journals Antipyretic effect of key lime (Citrus aurantiifolium) leaf extract on white rats

2012 ◽  
Vol 10 (2) ◽  
pp. 35-39
Author(s):  
AMELIA KARTIKA WIDOWATI ◽  
NUR HAFIDHA HIKMAYANI ◽  
ETI PONCORINI PAMUNGKASARI
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antipyretic Effect ◽  
White Rats ◽  
Key Lime ◽  
One Way Anova

Widowati AK, Hikmayani NH, Pamungkasari EP. 2012. Antipyretic effect of key lime (Citrus aurantifolia) leaf extract on white rats. Biofarmasi 10: 35-39. The objective of this study was to evaluate the antipyretic effect of key lime (Citrus aurantifolia L.) leaf extract on white rats (Rattus norvegicus) induced fever by using DPT vaccine. Thirty male white rats with the body weight of ±200 grams and the age of 2-3 months old were used in this study. Those rats were grouped equally into five groups, consisted of a negative control group (2 mL aquabidest), a positive control group (acetaminophen 6.3 mg/100 g of body weight), the first dose of key lime leaf extract (1.26 mg/100 g of body weight), the second dose (2.52 mg/100 g of body weight) and the third dose (5.04 mg/100 g of body weight). The measurement of rat temperature was conducted before and 2 hours after getting DPT vaccine, and every 30 minutes posttreatment until 120 minutes. Data at the 120th minute were analyzed by using One-Way Anova test. The results of One-Way Anova test showed that there were significant differences (p<0.05) among treatment groups. The results of post-hoc test analysis showed that the significant differences (p<0.05) were found between a negative control group and four other groups. There was no significant difference (p>0.05) between a positive control group and three groups of key lime leaf extract. It concluded that the key lime leaf extract has an antipyretic effect on white male rats.

scholarly journals UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) Dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) PADA TIKUS JANTAN PUTIH

2019 ◽  
Vol 2 (1) ◽  
pp. 24-28
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Purpose: To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide. Method: This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg/kgMB, group 3 single roselle extract dose 130 mg/kgMB, group 4 single meniran leaf extract dose 200 mg/kgMB, group 5 combination of meniran leaf extract dose of 100 mg/kgMB and rosella extract dose of 65 mg/kgMB, group 6 combination of meniran leaf extract dose of 200 mg/kgMB and rosella extract dose of 130 mg/kgMB, and group 7 meniran leaf extract combination dose 400 mg/kgMB and rosella extract dose 195 mg/kgMB. Result: The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella gave a significant effect compared to the negative control group did not have a significant effect in reducing blood glucose levels. Conclusion: Single meniran extract and high-dose combination extract are more effective than glibenclamide.

scholarly journals Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Rachmat Hidayat ◽  
Lusia Hayati
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Muscle Tissue ◽  
Leaf Extract ◽  
Negative Control ◽  
Diabetes Group ◽  
White Rats ◽  
Target Blood Glucose ◽  
Sugar Regulation ◽  
Sugar Levels

Diabetes mellitus is a chronic condition that disturbs the body's bloodsugar regulation. Bay leaves contain entirely various secondary metabolites,where this plant is rich in flavonoids, alkaloids, terpenes and glycosides. Thisstudy aims to assess the effect of bay leaf extract (Syzygium polyanthum) onblood sugar levels and the expression of GLUT4 protein in muscle tissue. A totalof 30 white rats (Rattus norvegicus) Wistar strain obtained from the EurekaResearch Laboratory (Palembang, Indonesia) weighing between 200 - 250 grams.Bay leaf simplicia was obtained from the Tawangmangu Herbal Research Center,Karanganyar, Indonesia. After 1 week of adaptation, the mice were randomlydivided into the following six groups, each containing 5 animals: Normal controlgroup, diabetes group (negative control), diabetes + metformin group (Met; 45mg / kg), Diabetes + BLE (75 mg/kg), diabetes + BLE group (150 mg / kg) anddiabetes + BLE group (300 mg/kg). Alloxan-induced white rats showed a verysignificant increase in blood sugar levels, where the use of the drug metforminwas able to reduce blood sugar levels significantly even though they had notreached the target blood glucose target of less than 200 mg / dL. The treatmentwith bay leaf extract was able to reduce blood sugar levels significantly. Theadministration of metformin drugs or bay leaf extract showed the ability toincrease the level of GLUT4 protein. In conclusion, bay leaf extract affectsreducing blood sugar levels in diabetes mellitus white rats by increasing glucoseintake to cells and tissues.

UJI EFEKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK DAUN MENIRAN (Phyllanthus niruri L.) dan KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) pada TIKUS JANTAN PUTIH SEBAGAI HEWAN PERCOBAAN

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Herviani Sari ◽  
Vera Estefania Kaban ◽  
Friska Raulina Situmorang ◽  
Firdaus Fahdi
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Background : Diabetes mellitus (DM) is a chronic disease caused by the body's inability to produce the hormone insulin or due to ineffective use of insulin production. Diabetes has become a global problem, DM prevalence of 4.6%, estimated in 2000 amounted to 5.6 million. One of the plants that can reduce blood glucose levels is meniran and rosella which are found in Indonesia. Objective : To determine the effect of decreasing blood glucose levels in white rats using a combination of meniran and rosella compared to glibenclamide.Method : This research was carried out experimentally. Simplicia of meniran leaves and rosella macerated using 80% ethanol. The research used 21 rats that were induced by alloxan and divided into 7 groups and all compared using glibenclamide.Group 1 (negative control) CMC Na 1%, group 2 (positive control) glibenclamide dose 0.45 mg / kgBB, group 3 single roselle extract dose 130 mg / kgBB, group 4 single meniran leaf extract dose 200 mg / kgBB, group 5 combination of meniran leaf extract dose of 100 mg / kgBB and rosella extract dose of 65 mg / kgBB, group 6 combination of meniran leaf extract dose of 200 mg / kgBB and rosella extract dose of 130 mg / kgBB, and group 7 meniran leaf extract combination dose 400 mg / kgBB and rosella extract dose 195 mg / kgBB. Result : The results showed that rats had hyperglycemia after being induced by alloxan.Data were analyzed using one way ANOVA method followed by LSD and tukeys' B post hoct test. Having a difference in the decrease in blood glucose levels between the positive control group and the five doses of meniran extract and rosella where p <0.05 gave a significant effect compared to the negative control group p> 0.05 did not have a significant effect in reducing blood glucose levels. Conclusion : Single meniran extract and high-dose combination extract are more effective than glibenclamide.

scholarly journals Pemberian Kombinasi Ekstrak Etanol Daun Pepaya (carica papaya l.) Pada Mencit

2019 ◽  
Vol 11 (02) ◽  
pp. 120-130
Author(s):  
Rani Prabandari
Keyword(s):  
Experimental Study ◽  
Mefenamic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Protection Mechanism ◽  
Natural Medicine ◽  
Relieve Pain ◽  
The World

The natural medicine has been known and used through out of the world. One of them is papaya. Efficacy papaya leave provide efficacy as fever, appetite enhancer, launsed menstruation and relieve pain. This study aims to determine whether the effect of aethanol extract of papaya leaf against power analgesic mefenamic acid on mice by using the method asetic acid induction. This study is an experimental study using twenty four mice. This animals were divided into six groups where each group consisting of four mice. Papaya leaves, mefenamic acid, and aquadest were give to each group as test substances and comparative solution. Observations were carried out for 10 minute using the method of asetic acid induction was a decreases of protection mechanism of the mice in of licking or stiff feet responses after the administration of test substances. There was a decrease of the response of mice to lick feet or stiff feet to the asetic acid induction were given after administration of papaya leaf extract. The extract of papaya leaves has an analgesic effect on mice.

scholarly journals EVALUATION OF MEMORY-ENHANCING ACTIVITY FOR ERYTHRININE FROM LEAF EXTRACT OF ERYTHRINA INDICA

2019 ◽  
pp. 101-103
Author(s):  
KAMALRAJ R ◽  
SUBHASHREE G R
Keyword(s):  
Normal Saline ◽  
Water Maze ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Test Method ◽  
Morris Water Maze Test ◽  
Memory Enhancement ◽  
Maze Test ◽  
Memory Enhancing

Objective: The objective of the study was to investigate the memory-enhancing activity for erythrinine (ring-c-oxygenated Erythrina alkaloid) from leaf extract of Erythrina indica in mice. Methods: The study protocol designed in the way that it was carried out for 21 successive days by administrating the 5 mg/kg, s.c doses of isolated erythrinine to mice and the profile was challenged against the mice feeds with normal saline as a positive control and the mice treated with 5 mg/kg, s.c corticosterone as a negative control for the amnesia using Morris water maze test method. Results: Erythrinine-administered mice are showing remarkable retention of the memory (9.07±0.52) on the 21st day as such of the positive control normal saline (10.14±0.22) and against the negative control corticosterone-injected mice (70.86±0.54). Conclusions: The dose of 5 mg/kg s.c of isolated erythrinine from the leaf of E. indica shows the remarkable memory enhancement when it was investigated with the memory weakening induced test by corticosterone in mice’s using 5 mg/kg s.c. However, further studies required to elucidate the exact mechanism of action for developing its as potent memory-enhancing drug.

Sign in / Sign up

Export Citation Format

Share Document