scholarly journals Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Rachmat Hidayat ◽  
Lusia Hayati
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Muscle Tissue ◽  
Leaf Extract ◽  
Negative Control ◽  
Diabetes Group ◽  
White Rats ◽  
Target Blood Glucose ◽  
Sugar Regulation ◽  
Sugar Levels

Diabetes mellitus is a chronic condition that disturbs the body's bloodsugar regulation. Bay leaves contain entirely various secondary metabolites,where this plant is rich in flavonoids, alkaloids, terpenes and glycosides. Thisstudy aims to assess the effect of bay leaf extract (Syzygium polyanthum) onblood sugar levels and the expression of GLUT4 protein in muscle tissue. A totalof 30 white rats (Rattus norvegicus) Wistar strain obtained from the EurekaResearch Laboratory (Palembang, Indonesia) weighing between 200 - 250 grams.Bay leaf simplicia was obtained from the Tawangmangu Herbal Research Center,Karanganyar, Indonesia. After 1 week of adaptation, the mice were randomlydivided into the following six groups, each containing 5 animals: Normal controlgroup, diabetes group (negative control), diabetes + metformin group (Met; 45mg / kg), Diabetes + BLE (75 mg/kg), diabetes + BLE group (150 mg / kg) anddiabetes + BLE group (300 mg/kg). Alloxan-induced white rats showed a verysignificant increase in blood sugar levels, where the use of the drug metforminwas able to reduce blood sugar levels significantly even though they had notreached the target blood glucose target of less than 200 mg / dL. The treatmentwith bay leaf extract was able to reduce blood sugar levels significantly. Theadministration of metformin drugs or bay leaf extract showed the ability toincrease the level of GLUT4 protein. In conclusion, bay leaf extract affectsreducing blood sugar levels in diabetes mellitus white rats by increasing glucoseintake to cells and tissues.

scholarly journals The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study

2020 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Lusia Hayati ◽  
Rachmat Hidayat
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Leaf Extract ◽  
Negative Control ◽  
Control Group ◽  
Diabetes Group ◽  
White Rats ◽  
Sugar Regulation ◽  
Sugar Levels

Sambiloto (Andrographis peniculata) is one of the most common plants inIndonesia. Sambiloto contains quite varied secondary metabolites, where thisplant is rich in flavonoids, alkaloids, terpenes and glycosides. This study aimsto assess the effect of Sambiloto (Andrographis peniculata) leaf extract on bloodsugar levels and the expression of GLUT4 protein in muscle tissue whichindicates the potential of the test extract's ability to improve blood glucose intaketo cells so that it can maintain blood sugar regulation. The process of extractingsambiloto is carried out by maceration in which 500 grams of simplicia aremacerated with 96% ethanol for 72 hours. After 1 week of adaptation, the micewere randomly divided into the following six groups, each containing 5 animals:Normal control group, diabetes group (negative control), diabetes + metformingroup (Met; 45 mg/kg), Diabetes + ES group (50 mg/kg), diabetes + ES group(100 mg/kg) and diabetes + ES group (200 mg/kg). The treatment with sambilotoextract was able to reduce blood sugar levels significantly, were at the ES 100and 200 mg/kg BW doses it was able to reduce blood sugar levels to reach thetarget below 200 mg/kg BW. The dominant flavonoids in Sambiloto leaf extractare believed to be responsible for the effect of blood glucose regulation. Inconclusion, Sambiloto extract affects lowering blood sugar levels in diabetesmellitus white rats by increasing glucose intake to cells and tissues.

scholarly journals Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue

2020 ◽  
Vol 1 (1) ◽  
pp. 9-16
Author(s):  
Patricia Wulandari
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Muscle Tissue ◽  
Morinda Citrifolia ◽  
Wild Plants ◽  
Fruit Extract ◽  
White Rats ◽  
Glucose Intake ◽  
Sugar Regulation ◽  
Sugar Levels

Noni fruit (Morinda citrifolia) is one of the most common plants inIndonesia. This plant is often found in various regions in Indonesia, where theseplants are often in the form of shrubs or wild plants that grow in yards orplantations. This study aims to assess the effect of noni (Morinda citrifolia) fruitextract on blood sugar levels and the expression of GLUT4 protein in muscletissue which shows the potential of the test extract's ability to improve bloodglucose intake to cells so that it can maintain blood sugar regulation. Thisresearch was an experimental study that used white rats as research subject.Induction of diabetes was done by injecting alloxan at a dose of 110 mg/kg BWintraperitoneally; then the white rats were given 10% glucose to drink. Alloxan-induced white rats showed a very significant increase in blood sugar levels,where the use of the drug metformin was able to reduce blood sugar levelssignificantly. In white rats induced with diabetes mellitus, there was a decreasein GLUT4 levels in muscle tissue. The administration of metformin drugs or nonifruit extracts shows the ability to increase levels of GLUT4 protein. In conclusion,noni fruit extract affects lowering blood sugar levels in diabetes mellitus whiterats by increasing glucose intake to cells and tissues.

scholarly journals UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 44-53
Author(s):  
Andri Priyoherianto ◽  
◽  
Panji Ratih Suci ◽  
Putri Rizki Cahya Fatimah ◽  
Atik Nur Wijayanti
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Water Hyacinth ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Normal Limits ◽  
Blood Glucose Levels ◽  
Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

scholarly journals Increased Plasma GLP-1 Levels after Resistant Starch Type 3 From Cassava Starch (Manihot Esculanta Crantz) Diet on Diabetic Rat

2018 ◽  
Vol 4 (2) ◽  
pp. 116
Author(s):  
Elly Nurus Sakinah
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Resistant Starch ◽  
Nutritional Therapy ◽  
Positive Control ◽  
Cassava Starch ◽  
Negative Control ◽  
Diabetic Rat ◽  
Sugar Levels ◽  
Type 3

Diabetes mellitus (DM) occur when human body is unable to produce enough insulin and/or unable to use insulin effectively, resulting in an increase of blood sugar levels (hyperglycemia). Indonesia  has  ranks  fourth  after  United  States,  China  and  India  in  the  prevalence  of  DM. Controling blood sugar and insulin levels can be done through the stimulation effect of Glucagon- like peptide-1 (GLP-1). The goal of this study was to determine the effects of resistant starch type 3 (RS3) from cassava starch in increasing plasma GLP-1 levels. This reasearch use male mouse (Ratttus novergicus) as a diabetic model by providing a high-fat diet (HFD) for one mounth than combine with intraoperitonel injection of low dose streptozotocin (STZ). The mouse then grouped into 4 groups randomly (1) normal or negative control, (2) cassava starch diet, (3) RS3 diet, (4) positive control. Blood sugar levels were measured before and after STZ injection to determine the diabetic conditions (blood sugar > 200 mg /dL). After 4 weeks of dietary administration blood sugar  and  plasma  GLP-1  levels  were  examined  using  ELISA.  Statistical  analysis  showed decreased in blood sugar levels and increased in plasma GLP-1 levels after one mounth of RS3 diet. This research shows that RS3 from cassava starch has a potential role as a nutritional therapy on diabetes mellitus condition. Keywords: Resistant starch type 3, cassava starch, GLP-1, Blood Sugar, Diabetes Mellitus

scholarly journals EFEKTIVITAS ANTIDIABETES EKSTRAK DAUN RAMBUSA (Passiflora foetida L.) PADA MENCIT (Mus musculus) DENGAN INDUKSI GLUKOSA

2015 ◽  
Vol 1 (2) ◽  
pp. 99-104
Author(s):  
Khildah Khaerati ◽  
Ihwan Ihwan ◽  
Musdalifah S Maya
Keyword(s):  
Blood Sugar ◽  
Leaf Extract ◽  
Drug Research ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antidiabetic Drug ◽  
Passiflora Foetida ◽  
Sugar Levels

Passiflora foetida leaves used by the society for generations as a natural antidiabetic drug research aims to test the effectiveness of the leaf extract of Passiflora foetida with variations of a dose of 250 mg / kg, 500 mg / kg, and 750 mg / kg in lowering blood sugar levels in mice. Passiflora foetida leaves were extracted by sokhletasi method with 70% ethanol, the extract was tested on 15 mice, the test animals were randomized into 5 groups consist of negative control group given 0.5% Na-CMC, positive control group was given glibenclamide 0, 65 mg / kg, and the treatment group were given the leaf extract of Passiflorafoetida with variations of each dose of 250 mg / kg, 500 mg / kg, 750 mg / kg. To increase blood sugar levels in mice induced glucose 50%. Measurement of blood sugar levels in mice were performed using an easy touch glucometer and blood is taken via the lateral tail vein. The results showed that the percentage of blood sugar levels drop to a negative control is 13.1%, for a dose of 250 mg / kg body weight of 29.52%, for a dose of 500 mg / kg was 38.79%, to dosis750 mg / kg was 49, 21%, and 48.1% for the positive control. The results showed that the leaf extract of Passiflorafoetida at a dose of 750 mg / kg is most effective as an antidiabetic

Improved cellular immunity and increased insulin in streptozotosin-induced mice using ethanol coriander (Coriandrum sativum) extract

2021 ◽  
pp. 3689-3694
Author(s):  
Dahliatul Qosimah ◽  
Sri Widyarti ◽  
MAG Beltran ◽  
Muhaimin Rifa’i
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Gastric Tube ◽  
Fat Metabolism ◽  
Relative Number ◽  
Positive Control ◽  
Negative Control ◽  
Coriandrum Sativum ◽  
Treatment Groups ◽  
Sugar Levels

An increase in blood sugar levels is an indication of diabetes mellitus (DM). DM is a metabolic disease characterized by disorders of carbohydrate and fat metabolism due to impaired activity, insulin secretion or both. Alternatively, the body's inability to secrete insulin. The purpose of this study was to determine the effect of coriander extract (Coriandrum sativum) on mice induced by streptozotocin (STZ) on the relative number of CD11b and insulin-IL6 cells. The study used Balb-C strain mice, aged 2.5-3 months, with five (5) treatment groups which were given STZ at a dose of 145mg/kg BW on the 8th day IP (Intraperitoneal) after adaptation for seven days, then add coriander extract with successive doses of 25 (T1), 50 (T2), and 100mg/kg BW (T3), negative control (healthy) (T4) and positive control (STZ) (T5) through the administration of intraperitoneal gastric tube. Blood Glucose is measured on the 13th day. Herbs are given on days 14-28. On the 29th day, the mice. An increase in blood sugar levels is an indication of diabetes mellitus (DM). DM is a metabolic disease characterized by disorders of carbohydrate and fat metabolism due to impaired activity, insulin secretion or both Alternatively, the body's inability to secrete insulin. The purpose of this study was to determine the effect of coriander extract (Coriandrum sativum) on mice induced by streptozotocin (STZ) on the relative number of CD11b and insulin-IL6 cells. The study used Balb-C strain mice, aged 2.5-3 months, with five (5) treatment groups which were given STZ at a dose of 145mg/kg BW on the 8th day IP (Intraperitoneal) after adaptation for seven days, then add coriander extract with successive doses of 25 (T1), 50 (T2), and 100mg/kg BW (T3), negative control (healthy) (T4) and positive control (STZ) (T5) through the administration of intraperitoneal gastric tube. Blood Glucose is measured on the 13th day. Herbs are given on days 14-28. On the 29th day, the mice were sacrificed Furthermore, the pancreas was taken for insulin examination, and the spleen was checked with the relative number of CD11b-IL6 and T CD4 cells using a flow cytometer. Data were quantitatively analyzed using the One Way ANOVA test (p≤0.05). The results showed that doses of coriander in T1 treatment causes an increase in insulin levels, decreased blood sugar levels, weight gain, proliferation and activation of CD4+ T cells, and decrease inflammation (CD11b cell-IL6) on the tissue. This research concludes that extracts of coriander can reduce Hyperglycemia in mice by modulation of cellular immunity and increased levels of insulin

scholarly journals Potential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against Intake of Glucose in Muscle Rats-Induced Diabetes Mellitus

2021 ◽  
Vol 2 (1) ◽  
pp. 68-72
Author(s):  
Rachmat Hidayat ◽  
Patricia Wulandari
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Secondary Metabolites ◽  
Protein Expression ◽  
Blood Sugar ◽  
Chronic Condition ◽  
Andrographis Paniculata ◽  
Glucose Intake ◽  
Sugar Regulation ◽  
Sugar Levels

Diabetes mellitus is a chronic condition that disturbs the body's blood sugarregulation. This disorder is characterized by a decrease in the ability of bodycells to intake glucose into cells. Due to the failure of cells in glucose intake,glucose buildup occurs in the extracellular, namely in the plasma. Thesemedicinal plants contain quite varied secondary metabolites, where theseplants are rich in flavonoids, alkaloids, terpenes and glycosides. The contentof these secondary metabolite compounds is believed to be rich in antioxidanteffects so that it has the effect of being able to suppress various oxidativestress conditions that cause damage to various organs due to blood sugardysregulation. The effect of Tinospora crispa, Andrographis paniculata,Cinamomum burmanii, Syzygium polyanthum and Momordica charantiaextracts on blood sugar levels and GLUT4 protein expression in muscle tissueshows the potential of the test extract's ability to improve blood glucose intaketo cells so as to maintain blood sugar regulation.

scholarly journals Effect of Giving Extract of Lannea Coromandelica (Lannea coromandelica (Houttuyn) Merrill) Leaf to Decrease the Blood Sugar Level of Mice (Mus musculus)

2019 ◽  
Vol 8 (4) ◽  
pp. 223-229
Author(s):  
Moh. Husaini ◽  
Supriadi Supriadi ◽  
Minarni R. Jura
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Leaf Extract ◽  
Negative Control ◽  
Animal Testing ◽  
Leaf Sample ◽  
Lannea Coromandelica ◽  
Sugar Levels ◽  
Tree Leaf

The Indian ash tree leaf (Lannea coromandelica (Houttuyn) Merrill) contains natural ingredients that can reduce blood glucose levels. This study aimed to determine the concentration of the Indian ash tree leaf extract which is the most effective to reduce blood sugar levels of mice. The leaf sample of the Indian ash tree plant was obtained from Talise sub-district, Mantikulore district, Palu, Central Sulawesi. The preparation of the extract was conducted by the maceration method. The animal testing used were 20 male mice that were induced by EDTA (ethylene diamine tetraacetic acid). Mice were divided into 5 groups as random with different treatments. Treatments I, II, and III were given an extract of 10, 20, and 40%, respectively, treatment IV was given Na-CMC as a negative control (-), and treatment V was given glibenclamide suspension as a positive control (+). The data obtained were analyzed by statistical analysis of variance analysis (ANOVA) and were continued by the Duncan test. The results showed that the Indian ash tree leaf extract can reduce the blood sugar levels of mice. The mean decreased blood glucose level at treatment I was 40.00 mg/dL, treatment II was 47.75 mg/dL, treatment III was 54.75 mg/dL, treatment IV was 1.75 mg/dL, and treatment V was 49.00 mg/dL. The conclusion of this research was that the Indian ash tree leaf extract could reduce the blood sugar level of mice with an effective concentration of 20% with α = 0.05.

scholarly journals AKTIVITAS ANTIHIPERGLIKEMIK PATI TAPIOKA TERMODIFIKASI EKSTRAK DAUN JAMBU BIJI MERAH PADA TIKUS DIABETES [ANTIHYPERGLICEMIC ACTIVITY OF TAPIOCA STARCH MODIFIED WITH RED GUAVA LEAF EXTRACT ON DIABETIC RAT]

2020 ◽  
Vol 43 (1) ◽  
pp. 1-10
Author(s):  
Elisa Diana Julianti ◽  
Nunung Nurjanah ◽  
Deddy Muchtadi ◽  
Nurheni Sri Palupi ◽  
Dewi Ratih Agungpriyono
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Leaf Extract ◽  
Diabetic Rat ◽  
Sprague Dawley ◽  
Tapioca Starch ◽  
Antihyperglycemic Activity ◽  
Sugar Levels ◽  
Guava Leaves ◽  
Guava Leaf

Guava leaves extract were known to be rich in polyphenols and had an antihyperglycemic activity. Carbohydrate source food modified with polyphenols had lower digestibility and glycemic index than native. For that reason, tapioca (Manihot utilissima) modified with guava leaves extract was expected for reducing the risk of diabetes mellitus. The antihyperglycemic activity of tapioca starch modified with red guava leaf extract and its native starch were evaluated in streptozotocin-induced diabetic Sprague Dawley rats aged 2 months (weight 175-250 g). Rats were fed with tapioca starch modified with 4 percent guava leaf extract and native tapioca for 35 days. The rats, the blood glucose level, and pancreatic Langerhans islets were assayed. The result showed that tapioca starch modified with 4 percent guava leaves extract had antihyperglycemic activity compared to its native starch. Tapioca starch modified with 4 percent guava leaves extract significantly increased beta-cell pancreas density and even though it did not significantly lower blood sugar levels in diabetic rats, but at the end of treatment, blood sugar levels were lower than the diabetic rat fed with native tapioca.ABSTRAK Ekstrak daun jambu biji diketahui kaya akan polifenol dan memiliki aktivitas antihiperglikemik. Modifikasi pangan sumber karbohidrat dengan polifenol memiliki daya cerna dan indeks glikemik lebih rendah dibanding aslinya. Oleh karena itu pati tapioka (Manihot utilissima) termodifikasi ekstrak daun jambu biji merah diharapkan dapat mengurangi risiko diabetes mellitus. Aktivitas antihiperglikemik dari pati tapioka termodifikasi dengan ekstrak daun jambu biji merah dan pati aslinya dievaluasi menggunakan tikus Sprague Dawley berusia 2 bulan (175-250 g) yang diinduksi dengan streptozotocin. Tikus diabetes diberi pakan tapioka terdimodifikasi 4 persen ekstrak daun jambu dan tapioka asli sebagai pembanding selama 35 hari. Parameter yang dinilai adalah glukosa darah dan gambaran histopatologis pankreas tikus. Hasil menunjukkan bahwa tapioka termodifikasi 4 persen ekstrak daun jambu memiliki aktivitas antihiperglikemik dibandingkan pati aslinya. Tapioka termodifikasi ekstrak daun jambu biji merah secara signifikan meningkatkan kepadatan sel beta pankreas dan meskipun tidak signifikan menurunkan kadar gula darah pada tikus diabetes, namun pada akhir perlakuan, kadar gula darah lebih rendah dibandingkan kelompok tikus diabetes yang diberi pakan tapioka asli.

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN BAWANG DAYAK (Eleutherine Palmifolia L. Merr) TERHADAP KADAR GULA DARAH MENCIT PUTIH (Mus muscullus) YANG DIINDUKSI GLUKOSA

2020 ◽  
Vol 5 (2) ◽  
pp. 288-297
Author(s):  
Eka Kumalasari ◽  
◽  
Syifa Maharani ◽  
Aditya Maulana Perdana Putra ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Test Group ◽  
Ethanol Extract ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Sugar Levels

Diabetes Mellitus (DM) is a collection of metabolic symptoms that arise in a person caused by an increase in blood glucose due to damage to insulin secretion or resistance to insulin or both. Diabetes mellitus medication can be either parenteral or oral which can cause side effects in long-term use such as mild diarrhea, bloating, fatigue acidosis, even hypoglycemic renal impairment. To prevent it can use alternative medicine using Dayak onion (Eleutherine palmifolia (L) Merr.). One of the active substances contained in the Dayak onion is the flavonoids. Flavonoids have antidiabetic activity through their functions as antioxidants. The study aims to determine the activity of Dayak scallion Extract (Eleutherine palmifolia (L) Merr.) To decrease blood sugar levels (Mus muscullus), which is glucose-induced. The research was conducted with research draft pre and post test with control group design. The mice used amounted to 25 male white mice that were divided into 5 groups i.e. negative control (CMC Na 0.5%), control positive (Glibenklamid 3 mg/kgBB), Dayak onion-leaf ethanol extract at a dose of 50 mg/kgBB, 100 mg/kgBB and 200 mg/kgBB. Observations of the decrease in blood sugar levels of each test group were conducted on the same day, 30 minutes, 60 and 90 after hyperglycemia. Results showed that Dayak onion leaves ethanol extract had activity as a lowering of blood sugar levels in glucose-induced white mice

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