Physicochemical Characteristics and Biological Activities of Rice and Amaranth Fermented by Bacillus subtilis KMKW4

2015 ◽  
Vol 44 (4) ◽  
pp. 540-548
Author(s):  
Su-Jin Yang ◽  
Rea-Hyun Lee ◽  
Joo-Heon Hong
Keyword(s):  
Bacillus Subtilis ◽  
Biological Activities ◽  
Physicochemical Characteristics

scholarly journals Influencing Factors on the Physicochemical Characteristics of Tea Polysaccharides

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3457
Author(s):  
Ting Hu ◽  
Peng Wu ◽  
Jianfeng Zhan ◽  
Weixin Wang ◽  
Junfeng Shen ◽  
...  
Keyword(s):  
Influencing Factors ◽  
Biological Activities ◽  
Structural Difference ◽  
Physicochemical Characteristics ◽  
Bioactive Constituents ◽  
Preparation Methods ◽  
Processing Technologies ◽  
Chemical Structures ◽  
Tea Polysaccharides ◽  
Fine Structures

Tea polysaccharides (TPSs) are one of the main bioactive constituents of tea with various biological activities such as hypoglycemic effect, antioxidant, antitumor, and immunomodulatory. The bioactivities of TPSs are directly associated with their structures such as chemical composition, molecular weight, glycosidic linkages, and conformation among others. To study the relationship between the structures of TPSs and their bioactivities, it is essential to elucidate the structure of TPSs, particularly the fine structures. Due to the vast variation nature of monosaccharide units and their connections, the structure of TPSs is extremely complex, which is also affected by several major factors including tea species, processing technologies of tea and isolation methods of TPSs. As a result of the complexity, there are few studies on their fine structures and chain conformation. In the present review, we aim to provide a detailed summary of the multiple factors influencing the characteristics of TPS chemical structures such as variations of tea species, degree of fermentation, and preparation methods among others as well as their applications. The main aspects of understanding the structural difference of TPSs and influencing factors are to assist the study of the structure and bioactivity relationship and ultimately, to control the production of the targeted TPSs with the most desired biological activity.

Study on Propolis Quality from China and Uruguay

2000 ◽  
Vol 55 (9-10) ◽  
pp. 778-784 ◽  
Author(s):  
Josep Serra Bonvehí ◽  
Francesc Ventura Coll
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Biological Activities ◽  
The State ◽  
Active Components ◽  
E Coli ◽  
Geographic Origins ◽  
Potential Activity ◽  
And Staphylococcus Aureus

Abstract The composition, bacteriostatic and ROO• -scavenging potential activities of fifteen propolis samples from various botanic and geographic origins were determined to obtain objective information related to propolis quality. Variance analysis showed significant differences (p ≤ 0.05) in the contents of polyphenols, flavonoids and active components between fresh and aged propolis. The state of the product (fresh or aged) could be differentiate by using flavonoid pattern and biological activities. A minimum propolis concentration of 80 μg/ml was required inhibit Bacillus subtilis and Staphylococcus aureus while 800 μg/ml was required to inhibit Escherichia coli using fresh propolis. Aged propolis inhibit B. subtilis and S. aureus at concentration of 100 μg/ml and E. coli at 1000 μg/ml. A minimum flavonoids percentage of 18 g/100 g and a maximum ROO• -scavenging potential activity of 4.3 μg/ml were determined in fresh propolis. Flavonoids levels in aged propolis were approximately 20% lower than in fresh propolis. A maximum flavonoids percentage of 19.8 g/100 g and a ROO•-scavenging potential activity between 5.7 to 6.4 μg/ml in aged propolis were quantified. Another objective was to assess the use of ROO•-scavenging potential activity in propolis quality.

scholarly journals in vitro Screening of Sesamum indicum Seeds for Antioxidant, Phytochemical and Biological Properties

2020 ◽  
Vol 32 (9) ◽  
pp. 2262-2266
Author(s):  
SHERAZ KHAN ◽  
SANIYA HASHIM KHAN ◽  
INAM ULLAH KHAN ◽  
WALI INAM ◽  
MUHAMMAD ASIM ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Sesamum Indicum ◽  
Total Antioxidant

Herbal medicines has been the most cost-effective and valuable medical practice to cure diseases and emphasize modern health care treatment. The present research was conducted to assess the biological activities of 10 fractions obtained from methanolic extract which was derived from dried seeds of Sesamum indicum. Antioxidant activity was assessed using DPPH radical scavenging, total antioxidant capacity and total reducing power assays. Highest free radical scavenging activity (80.3 ± 1.36%), total antioxidant capacity (104.7 ± 4.04 μg AAE/mg) and ferric reducing power activity (238.76 ± 1.23 μg AAE/mg) was shown by fraction SE. Fraction SE showed the highest phenolic contents (63.72 ± 1.5 μg GAE/mg) while fraction SG sample showed highest flavonoid contents (54.62 ± 2.61 μg QE QE/mg). Antibacterial activity was performed against four selected bacterial strains including Staphylococcus aureus, Escherichia coli, Enterobacter aerogens and Bacillus subtilis. Highest inhibition was shown by fraction SD (11 ± 1.04 mm) against Staphylococcus aureus, fraction SH against Bacillus subtilis (11 ± 1.06 mm) and fraction SB against Escherichia coli. All fractions were found inactive against the selected fungal strains. While performing antileishmanial activity, fraction SC showed highest percent mortality (78%) of Leishmania tropica. In brine shrimp lethality bioassay, fraction SG showed significant LD50 value (23.48 μg/mL).

scholarly journals Genome mining and UHPLC-MS/MS illuminate the specificity of secondary metabolite synthetic gene clusters in Bacillus subtilis NCD-2

2020 ◽  
Author(s):  
Zhenhe Su ◽  
Xiuye Chen ◽  
Xiaomeng Liu ◽  
Qinggang Guo ◽  
Shezeng Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Biological Activities ◽  
Genome Mining ◽  
Gene Clusters ◽  
Synthetic Gene ◽  
Integrative Approach ◽  
Amino Acid Sequence Similarity ◽  
Genetic Characteristics

Abstract Background Bacillus subtilisstrain NCD-2 is anexcellent biocontrol agent against plant soil-borne diseases and shows broad-spectrum antifungal activities. This study aimed to explore all the secondary metabolite synthetic gene clusters and related bioactive compounds in NCD-2. An integrative approach, which coupled genome mining with structural identification technologies using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-MS/MS), was conducted to interpret the chemical origins of the significant biological activities in NCD-2. Results Genome mining revealed that NCD-2 contained nine gene clustershaving predicted functionsinvolving secondary metabolites with bioactive abilities. They encoded six known products-fengycin, surfactin, bacillaene, subtilosin, bacillibactin, and bacilysin-as well as three unknown products.Interestingly, the synthetic gene clusters for surfactin and fengycin showed 83% and 92% amino acid sequence similarity levels with the corresponding productsin Bacillus velezensisstrain FZB42. A further comparison of gene clusters encoding fengycin and surfactinrevealed that strain NCD-2 had lost thefenC and fenDgenes in the fengycinbiosynthetic operon, and that the surfactin synthetic enzyme-related gene srfAB was divided into two parts.Abioinformatics analysis showed that fenEAmay function as fenCD in synthesizing fengycinand that the structure of thisfengycin synthetic gene clusteris likely unique to NCD-2.Five kinds of fengycin,with 26 homologs, and surfactin,with 4 homologs,were detectedfrom strain NCD-2, which indicated the non-typical and unique nature of this fengycin biosynthetic gene cluster.To the best of our knowledge, this is the first report of a non-typical gene cluster related to fengycin synthesis. Conclusions The data provide the genetic characteristics of secondary metabolite synthetic gene clusters for fengycinand surfactin in B. subtilis NCD-2, including the unique synthetic mechanism for fengycin, and suggest that bioactive secondary metabolites explain the biological activities of NCD-2.

scholarly journals Phenylpropanoids and Furanocoumarins as Antibacterial and Antimalarial Constituents of the Bhutanese Medicinal Plant Pleurospermum amabile

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Phurpa Wangchuk ◽  
Stephen G. Pyne ◽  
Paul A. Keller ◽  
Malai Taweechotipatr ◽  
Sumalee Kamchonwongpaisan
Keyword(s):  
Antibacterial Activity ◽  
Plasmodium Falciparum ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Antimalarial Activity ◽  
Biological Activities ◽  
Multidrug Resistant ◽  
Scientific Data ◽  
Methyl Eugenol ◽  
Weak Antibacterial Activity

With the objective of determining safety and verifying the traditional uses of the Bhutanese medicinal plant, Pleurospermum amabile Craib & W. W. Smith, we investigated its crude extracts and the isolated phytochemicals for their biological activities. Four phenylpropanoids [( E)-isomyristicin (1), ( E)-isoapiol (2), methyl eugenol (3) and ( E)-isoelemicin (4)] and six furanocoumarins [psoralen (5), bergapten (6), isoimperatorin (7), isopimpinellin (8), oxypeucedanin hydrate (9) and oxypeucedanin methanolate (10)] were isolated from this plant. Among the test samples, compound 10 showed weak antibacterial activity against Bacillus subtilis and best antimalarial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). None of the test samples showed cytotoxicity. This study generated scientific data that support the traditional medical uses of the plant.

scholarly journals Biological activities of a mixture of biosurfactant from Bacillus subtilis and alkaline lipase from Fusarium oxysporum

2011 ◽  
Vol 42 (1) ◽  
pp. 354-361 ◽  
Author(s):  
Cedenir Pereira de Quadros ◽  
Marta Cristina Teixeira Duarte ◽  
Gláucia Maria Pastore
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Biological Activities ◽  
Alkaline Lipase
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