Manual para aplicar rociado residual intradomiciliario en zonas urbanas para el control de Aedes aegypti

2019 ◽

Keyword(s):

La gravedad de la situación epidemiológica reciente en Latinoamérica, con la cocirculación de los virus del dengue, del chikungunya y de la fiebre de Zika, la aparición de casos de microcefalia y otros padecimientos asociados (p. ej., el síndrome de Guillain-Barré) y la emergencia de epizootias de fiebre amarilla, motivaron la declaración de emergencia en las Américas por la Organización Mundial de la Salud en 2016.1 Ante la ausencia de un tratamiento específico y de vacunas contra el dengue, el chikungunya y el Zika, y considerando las limitaciones de las estrategias actuals de control vectorial, se urgió a incrementar y complementar las lternativas disponibles para mejorar el control del mosquito vector Aedes aegypti. Además, existe la dificultad de mantener coberturas de vacunación homogéneas y adecuadas contra la fiebre amarilla en centros urbanos endémicos, lo cual conlleva el riesgo de la circulación urbana de dicha enfermedad … Manual para aplicar rociado residual intradomiciliario en zonas urbanas para el control de Aedes aegypti no solo está dirigido al personal operativo y los mandos medios y directivos de los programas de prevención y control de las enfermedades transmitidas por Aedes, sino también a la comunidad académica relacionada con la investigación operativa sobre RRI-Aedes, a los controladores de plagas privados y al público en general.

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A Simple PCR Assay for Discrimination of Dengue Vectors from Nsukka LGA, Nigeria

International Annals of Science ◽

10.21467/ias.10.1.67-77 ◽

2020 ◽

Vol 10 (1) ◽

pp. 67-77

Author(s):

Amos Watentena ◽

Ikem Chris Okoye ◽

Ikechukwu Eugene Onah ◽

Onwude Cosmas Ogbonnaya ◽

Emmanuel Ogudu

Keyword(s):

Aedes Aegypti ◽

Aedes Albopictus ◽

Mosquito Species ◽

Public Health Problem ◽

Major Public Health Problem ◽

Mosquito Vector ◽

Aedes Mosquitoes ◽

Allele Specific ◽

Arboviral Diseases ◽

Dengue Vectors

Mosquitoes of Aedes species are vectors of several arboviral diseases which continue to be a major public health problem in Nigeria. This study among other things, morphologically identified Aedes mosquitoes collected from Nsukka LGA and used an allele specific PCR amplification for discrimination of dengue vectors. Larval sampling, BG-sentinel traps and modified human landing catches were used for mosquito sampling in two selected autonomous communities of Nsukka LGA (Nsukka and Obimo). A total of 124 Aedes mosquitoes consisting of five (5) different species were collected from April to June, 2019 in a cross-sectional study that covered 126 households, under 76 distinct geographical coordinates. Larvae was mainly collected from plastic containers 73% (n=224), metallic containers 14% (n=43), earthen pots 9% (n=29) and used car tyres 3% (n=9), reared to adult stage 69.35% (n=86), and all mosquitoes were identified using standard morphological keys. Five (5) Aedes mosquito species were captured; Aedes aegypti 83(66.94%), Aedes albopictus 33(26.61%), Aedes simpsoni (4.48%), Aedes luteocephalus (≤1%) and Aedes vittatus (≤1%). Nsukka autonomous community had higher species diversity than Obimo. Allele specific amplification confirmed dengue vectors, Aedes aegypti and Aedes albopictus species on a 2% agarose gel. Since the most recent re-emergence of arboviral diseases is closely associated with Aedes species, findings of this study, therefore, give further evidence about the presence of potential arboviral vectors in Nigeria and describe the role of a simple PCR in discriminating some. Further entomological studies should integrate PCR assays in mosquito vector surveillance.

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Blue Tansy Essential Oil: Chemical Composition, Repellent Activity Against Aedes aegypti and Attractant Activity for Ceratitis capitata

Natural Product Communications ◽

10.1177/1934578x21990194 ◽

2021 ◽

Vol 16 (2) ◽

pp. 1934578X2199019 ◽

Cited By ~ 1

Author(s):

Iris Stappen ◽

Juergen Wanner ◽

Nurhayat Tabanca ◽

Ulrich R. Bernier ◽

Paul E. Kendra

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Ceratitis Capitata ◽

Stationary Phases ◽

Management Strategies ◽

Fruit Fly ◽

Effective Dosage ◽

Mosquito Vector ◽

Tea Tree ◽

Potential Applications

Blue tansy essential oil (BTEO) ( Tanacetum annuum L.) was analyzed by GC-MS and GC-FID using two different capillary column stationary phases. Sabinene (14.0%), camphor (13.6%), myrcene (8.0%), β-pinene (7.7%), and chamazulene (6.9%) were the main components using an SE52 column (non-polar). On a polar CW20M phase column, sabinene (15.1%), camphor (14.4%), α-phellandrene (7.9%), β-pinene (7.7%), and myrcene (6.9%) were the most abundant compounds. To assess the oil for potential applications in integrated pest management strategies, behavioral bioassays were conducted to test for repellency against yellow fever mosquito Aedes aegypti, and for attractant activity for Mediterranean fruit fly Ceratitis capitata. Results showed that BTEO was not effective in repelling Ae. aegypti (minimum effective dosage [MED]: 0.625 ± 0.109 mg/cm2 compared with the standard insect repellent DEET (N,N-diethyl-3-methylbenzamide). In assays with male C. capitata, BTEO displayed mild attraction compared with two positive controls (essential oils from tea tree Melaleuca alternifolia and African ginger bush Tetradenia riparia). Additional studies are needed to identify the specific attractant chemicals in BTEO and to determine if they confer a synergistic effect when combined with other known attractants for C. capitata. To the best of our knowledge, this study represents the first investigation of BTEO for repellency against the mosquito vector Ae. aegypti and for attractancy to C. capitata, a major agricultural pest worldwide.

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Impact of Wolbachia on Infection with Chikungunya and Yellow Fever Viruses in the Mosquito Vector Aedes aegypti

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0001892 ◽

2012 ◽

Vol 6 (11) ◽

pp. e1892 ◽

Cited By ~ 205

Author(s):

Andrew F. van den Hurk ◽

Sonja Hall-Mendelin ◽

Alyssa T. Pyke ◽

Francesca D. Frentiu ◽

Kate McElroy ◽

...

Keyword(s):

Aedes Aegypti ◽

Yellow Fever ◽

Mosquito Vector

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Sequential Infection of Aedes aegypti Mosquitoes with Chikungunya Virus and Zika Virus Enhances Early Zika Virus Transmission

Insects ◽

10.3390/insects9040177 ◽

2018 ◽

Vol 9 (4) ◽

pp. 177 ◽

Cited By ~ 12

Author(s):

Tereza Magalhaes ◽

Alexis Robison ◽

Michael Young ◽

William Black ◽

Brian Foy ◽

...

Keyword(s):

Aedes Aegypti ◽

Blood Meal ◽

Zika Virus ◽

Chikungunya Virus ◽

Vector Competence ◽

Virus Transmission ◽

Mosquito Vector ◽

Molecular Aspects ◽

Virus Interactions ◽

Sequential Infection

In urban settings, chikungunya, Zika, and dengue viruses are transmitted by Aedes aegypti mosquitoes. Since these viruses co-circulate in several regions, coinfection in humans and vectors may occur, and human coinfections have been frequently reported. Yet, little is known about the molecular aspects of virus interactions within hosts and how they contribute to arbovirus transmission dynamics. We have previously shown that Aedes aegypti exposed to chikungunya and Zika viruses in the same blood meal can become coinfected and transmit both viruses simultaneously. However, mosquitoes may also become coinfected by multiple, sequential feeds on single infected hosts. Therefore, we tested whether sequential infection with chikungunya and Zika viruses impacts mosquito vector competence. We exposed Ae. aegypti mosquitoes first to one virus and 7 days later to the other virus and compared infection, dissemination, and transmission rates between sequentially and single infected groups. We found that coinfection rates were high after sequential exposure and that mosquitoes were able to co-transmit both viruses. Surprisingly, chikungunya virus coinfection enhanced Zika virus transmission 7 days after the second blood meal. Our data demonstrate heterologous arbovirus synergism within mosquitoes, by unknown mechanisms, leading to enhancement of transmission under certain conditions.

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Tumbuhan Obat: Pendekatan Alternatif dalam Pengendalian Nyamuk Aedes aegypti dan Virus Dengue Penyebab Demam Dengue

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.541 ◽

2018 ◽

Vol 4 (2) ◽

pp. 83-90

Author(s):

Ign Joko Suyono ◽

Aditya K. Karim

Keyword(s):

Medicinal Plant ◽

Aedes Aegypti ◽

Dengue Fever ◽

Mosquito Control ◽

Dengue Shock Syndrome ◽

Viral Disease ◽

Mosquito Vector ◽

Major Health ◽

Alternative Approach ◽

Control And Prevention

Dengue is the most important emerging tropical viral disease of humans in the world today. Aedes aegypti is a major mosquito vector responsible for transmitting many viral diseases and this mosquito that spreads major health problems like dengue fever. The resistance of Ae. aegypti to insecticides is already widespread and represents a serious problem for programmes aimed at the control and prevention of dengue in tropical countries. The search for compounds extracted from medicinal plant preparations as alternatives insecticide for mosquito control is in immediate need. Alternative approach for control Ae. aegypti dan virus dengue using the medicinal plant will be discussed in this paper.Key words: Medicinal plant, Aedes aegypti, dengue fever, dengue haemorragi fever, dengue shock syndrome

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Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes

Frontiers in Immunology ◽

10.3389/fimmu.2021.660873 ◽

2021 ◽

Vol 12 ◽

Author(s):

Thiago H. J. F. Leite ◽

Álvaro G. A. Ferreira ◽

Jean-Luc Imler ◽

João T. Marques

Keyword(s):

Aedes Aegypti ◽

Cellular Responses ◽

Innate Immune ◽

Mosquito Vector ◽

Phagocytic Function ◽

Systemic Dissemination ◽

Latex Beads ◽

Medical Importance ◽

Immune Pathways

Aedes aegypti mosquitoes are vectors for arboviruses of medical importance such as dengue (DENV) and Zika (ZIKV) viruses. Different innate immune pathways contribute to the control of arboviruses in the mosquito vector including RNA interference, Toll and Jak-STAT pathways. However, the role of cellular responses mediated by circulating macrophage-like cells known as hemocytes remains unclear. Here we show that hemocytes are recruited to the midgut of Ae. aegypti mosquitoes in response to DENV or ZIKV. Blockade of the phagocytic function of hemocytes using latex beads induced increased accumulation of hemocytes in the midgut and a reduction in virus infection levels in this organ. In contrast, inhibition of phagocytosis by hemocytes led to increased systemic dissemination and replication of DENV and ZIKV. Hence, our work reveals a dual role for hemocytes in Ae. aegypti mosquitoes, whereby phagocytosis is not required to control viral infection in the midgut but is essential to restrict systemic dissemination. Further understanding of the mechanism behind this duality could help the design of vector-based strategies to prevent transmission of arboviruses.

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Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2014.31.31-33 ◽

2014 ◽

Vol 3 (1) ◽

pp. 31

Author(s):

Rahmawati Ekaputri ◽

Sudarsono Sudarsono ◽

Budi Mulyaningsih

Keyword(s):

Aedes Aegypti ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Larvicidal Activity ◽

Larval Mortality ◽

Probit Analysis ◽

Mosquito Vector ◽

Fruit Extract ◽

Vinca Rosea ◽

Layer Chromatography

Background: Vinca rosea is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of Vinca rosea was selected to investigation its larvicidal activity against the 3rd instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) Aedes aegypti. Five concentrations of Vinca fruit extract were tested against the 3rd instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC50 anf LC90) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC50 and LC90 values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.

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Co-occurrence of kdr Mutations V1016I and F1534C and Its Association With Phenotypic Resistance to Pyrethroids in Aedes aegypti (Diptera: Culicidae) Populations From Costa Rica

Journal of Medical Entomology ◽

10.1093/jme/tjz241 ◽

2019 ◽

Vol 57 (3) ◽

pp. 830-836

Author(s):

Aryana Zardkoohi ◽

David Castañeda ◽

Juan C Lol ◽

Carmen Castillo ◽

Francisco Lopez ◽

...

Keyword(s):

Costa Rica ◽

Aedes Aegypti ◽

Insecticide Resistance ◽

Vector Control ◽

Strong Association ◽

Resistance Management ◽

Management Plan ◽

Costa Rican ◽

Mosquito Vector ◽

Phenotypic Resistance

Abstract Aedes aegypti (Linnaeus, 1762) is considered the most important mosquito vector species for several arboviruses (e.g., dengue, chikungunya, Zika) in Costa Rica. The primary strategy for the control and prevention of Aedes-borne diseases relies on insecticide-based vector control. However, the emergence of insecticide resistance in the mosquito populations presents a significant threat to these prevention actions. The characterization of the mechanisms driving the insecticide resistance in Ae. aegypti is vital for decision making in vector control programs. Therefore, we analyzed the voltage-gated sodium channel (VGSC) gene for the presence of the V1016I and F1534C kdr mutations in Ae. aegypti populations from Puntarenas and Limon provinces, Costa Rica. The CDC bottle bioassays showed that both Costa Rican Ae. aegypti populations were resistant to permethrin and deltamethrin. In the case of kdr genotyping, results revealed the co-occurrence of V1016I and F1534C mutations in permethrin and deltamethrin-resistant populations, as well as the fixation of the 1534C allele. A strong association between these mutations and permethrin and deltamethrin resistance was found in Puntarenas. Limon did not show this association; however, our results indicate that the Limon population analyzed is not under the same selective pressure as Puntarenas for the VGSC gene. Therefore, our findings make an urgent call to expand the knowledge about the insecticide resistance status and mechanisms in the Costa Rican populations of Ae. aegypti, which must be a priority to develop an effective resistance management plan.

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