scholarly journals Blue Tansy Essential Oil: Chemical Composition, Repellent Activity Against Aedes aegypti and Attractant Activity for Ceratitis capitata

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199019 ◽  
Author(s):  
Iris Stappen ◽  
Juergen Wanner ◽  
Nurhayat Tabanca ◽  
Ulrich R. Bernier ◽  
Paul E. Kendra
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Ceratitis Capitata ◽  
Stationary Phases ◽  
Management Strategies ◽  
Fruit Fly ◽  
Effective Dosage ◽  
Mosquito Vector ◽  
Tea Tree ◽  
Potential Applications

Blue tansy essential oil (BTEO) ( Tanacetum annuum L.) was analyzed by GC-MS and GC-FID using two different capillary column stationary phases. Sabinene (14.0%), camphor (13.6%), myrcene (8.0%), β-pinene (7.7%), and chamazulene (6.9%) were the main components using an SE52 column (non-polar). On a polar CW20M phase column, sabinene (15.1%), camphor (14.4%), α-phellandrene (7.9%), β-pinene (7.7%), and myrcene (6.9%) were the most abundant compounds. To assess the oil for potential applications in integrated pest management strategies, behavioral bioassays were conducted to test for repellency against yellow fever mosquito Aedes aegypti, and for attractant activity for Mediterranean fruit fly Ceratitis capitata. Results showed that BTEO was not effective in repelling Ae. aegypti (minimum effective dosage [MED]: 0.625 ± 0.109 mg/cm2 compared with the standard insect repellent DEET (N,N-diethyl-3-methylbenzamide). In assays with male C. capitata, BTEO displayed mild attraction compared with two positive controls (essential oils from tea tree Melaleuca alternifolia and African ginger bush Tetradenia riparia). Additional studies are needed to identify the specific attractant chemicals in BTEO and to determine if they confer a synergistic effect when combined with other known attractants for C. capitata. To the best of our knowledge, this study represents the first investigation of BTEO for repellency against the mosquito vector Ae. aegypti and for attractancy to C. capitata, a major agricultural pest worldwide.

scholarly journals Chemical Composition of Essential Oil From Tetradenia riparia and Its Attractant Activity for Mediterranean Fruit Fly, Ceratitis capitata

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095395
Author(s):  
Eugene K. Blythe ◽  
Nurhayat Tabanca ◽  
Betul Demirci ◽  
Paul E. Kendra
Keyword(s):  
Essential Oil ◽  
Ceratitis Capitata ◽  
Chemical Constituents ◽  
Fruit Fly ◽  
Positive Control ◽  
Mediterranean Fruit Fly ◽  
Great Promise ◽  
Tea Tree ◽  
Flame Ionization ◽  
Aerial Parts

The Mediterranean fruit fly or medfly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the most economically important invasive pests worldwide, with over 300 known hosts. Essential oils have great promise for application in integrated pest management, where they function as natural repellents, attractants, and toxicants with a reduced impact on the environment. In this study, we evaluated essential oil from aerial parts of the African ginger bush, Tetradenia riparia (Hochst.) Codd (Lamiaceae), as a potential new attractant for male C. capitata. Tetradenia riparia essential oil (TREO) was analyzed by gas chromatography (GC)-flame ionization detection and GC-mass spectrometry (GC-MS). The primary compounds identified were fenchone (15%), δ-cadinene (11%), 14-hydroxy-β-caryophyllene (8%), and tau-cadinol (7%). In short-range laboratory bioassays with sterile male medflies, TREO exhibited attractancy comparable to that observed with a positive control, essential oil from tea tree ( Melaleuca alternifolia (Maiden and Betche) Cheel.). This study provides the first report of C. capitata behavioral response to TREO. Further research is needed, particularly with the 2 enantiomers of fenchone, to determine the chemical constituents responsible for the attraction of C. capitata.

scholarly journals Insecticidal and Attractant Activities of Magnolia citrata Leaf Essential Oil against Two Major Pests from Diptera: Aedes aegypti (Culicidae) and Ceratitis capitata (Tephritidae)

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2311
Author(s):  
Ngoc Anh Luu-Dam ◽  
Nurhayat Tabanca ◽  
Alden S. Estep ◽  
Duy Hung Nguyen ◽  
Paul E. Kendra
Keyword(s):  
Puerto Rico ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Insecticidal Activity ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Positive Control ◽  
Sterile Male ◽  
Leaf Essential Oil ◽  
First Time

In this study, Magnolia citrata Noot and Chalermglin (Magnoliaceae) essential oil (MCEO) was evaluated for insecticidal activity against the yellow fever mosquito Aedes aegypti and attractant activity for the Mediterranean fruit fly Ceratitis capitata. The leaves of Magnolia citrata (Giổi chanh) were collected from northwestern Vietnam, and the water-distilled MCEO was analyzed by gas-chromatography and mass spectrometry (GC-MS). The major constituents of MCEO were identified as linalool 19%, geranial 16%, citronellal 14%, neral 14%, and sabinene 12%. MCEO showed 100% mortality at 1 μg/μL against 1st instar larvae of Ae. aegypti (Orlando strain, ORL), and the oil exhibited 54% (ORL) and 68% (Puerto Rico strain) mortality at 5 μg/mosquito against Ae. aegypti adult females. Initial screens showed that MCEO had weak insecticidal activity compared to the positive control permethrin. In bioassays with sterile male C. capitata, MCEO exhibited moderately strong attraction, comparable to that observed with a positive control, Tetradenia riparia essential oil (TREO). Herein, the insecticidal and attractant activities of MCEO are reported for the first time.

scholarly journals TLC-Based Bioassay to Isolate Kairomones from Tea Tree Essential Oil That Attract Male Mediterranean Fruit Flies, Ceratitis capitata (Wiedemann)

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 683
Author(s):  
Nurhayat Tabanca ◽  
Jerome Niogret ◽  
Paul E. Kendra ◽  
Nancy D. Epsky
Keyword(s):  
Essential Oil ◽  
Short Range ◽  
Ceratitis Capitata ◽  
Control Strategies ◽  
The United States ◽  
Rapid Screening ◽  
Detection Methods ◽  
Invasive Pest ◽  
Vegetable Production ◽  
Tea Tree

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) poses a major threat to fruit and vegetable production in the United States and throughout the world. New attractants and detection methods could improve control strategies for this invasive pest. In this study, we developed a method that combined thin-layer chromatography (TLC) of tea tree essential oil (TTO) (Melaleuca alternifolia) with short-range bioassays to isolate attractive kairomones for male C. capitata. After development, the TLC chromatogram indicated that TTO separated into five major spots, designated as zones 1 to 5. When the TLC plate was exposed to flies, zones 1 and 3 were strongly attractive to male C. capitata. To confirm activity, the developed TLC plate was cut into five zones which were then tested in short-range bioassays. Again, flies were observed to aggregate around zones 1 and 3, which corresponded with Rf values of 0.93 and 0.59. In addition, zones 1 to 5 were separated using preparative-TLC, and olfactory responses to volatile emissions from the five fractions were quantified by electroantennography (EAG). Highest amplitude EAG responses were recorded with fractions 1 and 3, further supporting the bioactivity of these samples. In conclusion, a TLC-based bioassay system can provide an effective, rapid screening protocol for initial isolation of insect kairomones from complex mixtures such as essential oils or plant extracts. Further analysis of TTO fractions 1 and 3 is needed to identify the specific constituents attractive to male C. capitata.

scholarly journals Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

2021 ◽  
Vol 15 (7) ◽  
pp. e0009587
Author(s):  
Ivana Sierra ◽  
Jose Manuel Latorre-Estivalis ◽  
Lucila Traverso ◽  
Paula V. Gonzalez ◽  
Ariel Aptekmann ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Vector Control ◽  
Gene Families ◽  
Control Group ◽  
Mosquito Vector ◽  
Rna Seq ◽  
Chemosensory Proteins ◽  
The Impact

Background Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

Toxic effect of essential oil and its compounds isolated from Sphaeranthus amaranthoides Burm. f. against dengue mosquito vector Aedes aegypti Linn.

2019 ◽  
Vol 160 ◽  
pp. 163-170 ◽  
Author(s):  
Annamalai Thanigaivel ◽  
Kanagaraj Muthu-Pandian Chanthini ◽  
Sengodan Karthi ◽  
Prabhakaran Vasantha-Srinivasan ◽  
Athirstam Ponsankar ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Toxic Effect ◽  
Mosquito Vector

scholarly journals Pemanfaatan Jeruk Purut (Citrus Hystrix) Sebagai Biolarvasida

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Titik Lestari
Keyword(s):  
Essential Oil ◽  
Literature Review ◽  
Aedes Aegypti ◽  
Research Methods ◽  
Chemical Compound ◽  
Mosquito Vector ◽  
The Third ◽  
Chemical Content ◽  
Lethal Time ◽  
Third Instar Larvae

Absctract: Citrus hystrix, Biolarvicides. Insectisides and pestisides were used as larvicides to control mosquito vector Aedes aegypti particularly. Some research suggested there had been a larvae resistance to pesticides. Biolarvicides with basic ingredients plant was another alternative that can be used. Lime (Citrus hystrix) was one of plants that often used as biolarvicides, both lits fruit and leaves. This paper aimed to know about chemical compound in lime and its potential as biolarvicidal. Research methods was literature review from several research journals. The results showed that the presence of coumpounds were essential oil, flavonoids, saponins, terpeesn, limonoids, citronellal, linalool, citronellol, acetate citronelil, kariofilin and geraniol. LC50 of lime leaves as biolarvicides againts the third instar larvae of Aedes aegypti was 279.882 ppm, biolarvicides oxicity values (LC90) was 4000 ppm with the lethal time (LT90) was 12.67 hours. Based on results of this study concluded that the presence of the chemical content of Citrus hystrix plant potential as biolarvicides.

scholarly journals Differential Responses of Ceratitis Capitata to Infection by the Entomopathogenic Fungus Purpureocillium Lilacinum.

2021 ◽  
Author(s):  
Wafa Djobbi ◽  
Meriem Msaad Guerfali ◽  
Agnès Vallier ◽  
Kamel Charaabi ◽  
Justin Maire ◽  
...  
Keyword(s):  
Entomopathogenic Fungus ◽  
Ceratitis Capitata ◽  
Sublethal Effects ◽  
Glycosyl Hydrolase ◽  
Fruit Fly ◽  
Over Expression ◽  
Purpureocillium Lilacinum ◽  
Potential Applications ◽  
Metabolic Activities ◽  
Nutrient Reserves

Abstract Ceratitis capitata (medfly), is one of the most injurious pests of fruits with quarantine importance because of its extremely wide host range. The use of entomopathogenic fungi constitutes a promising approach for potential applications in integrated pest management. Nonetheless, developing methods of insect control can also involve the use of fungal machinery to produce metabolic disturbance that can increase its effectiveness by producing a detrimental effect on insect development. Insect species, such as Ceratitis capitata, depend on reproduction potential, nutrient reserves, metabolic activities and immune response for their survival. Accordingly, the purpose of this study is to use the entomopathogenic fungus Purpureocillium lilacinum to investigate, its sublethal effects on Ceratitis capitata. Laboratory bioassays were conducted on medfly V8 strain. The bioassays were monitored to determine the virulence of P. lilacinum on the fruit fly. P. lilacinum was tested against 5 days-old males and females, through abdominal topical applications. Following the fungal inoculation, we showed (i) a significant increase of sugar amount in tissues, (ii) a significant decrease in carbohydrase activities, digestive glycosyl hydrolase and proteinase activities in whole midguts of treated flies, (iii) an over-expression of Takeout and Attacin-A genes induced by infection. Moreover, the up-regulations observed for relish, cecropin 1, ceratotox-A and defensin genes are due to physiological mechanisms occurring during infection.

Toxicity of some essential oil formulations against the Mediterranean fruit fly Ceratitis capitata (Wiedemann) (Diptera Tephritidae)

2012 ◽  
Vol 42 ◽  
pp. 223-229 ◽  
Author(s):  
Giovanni Benelli ◽  
Guido Flamini ◽  
Angelo Canale ◽  
Pier Luigi Cioni ◽  
Barbara Conti
Keyword(s):  
Essential Oil ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
The Mediterranean ◽  
Essential Oil Formulations

scholarly journals Lantana montevidensis Essential Oil: Chemical Composition and Mosquito Repellent Activity against Aedes aegypti

2016 ◽  
Vol 11 (11) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Eugene K. Blythe ◽  
Nurhayat Tabanca ◽  
Betul Demirci ◽  
Maia Tsikolia ◽  
Jeffrey R. Bloomquist ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Positive Control ◽  
Repellent Activity ◽  
Effective Dosage ◽  
Mosquito Repellent ◽  
Plant Parts ◽  
Sesquiterpene Hydrocarbons

The essential oil (EO) of Lantana montevidensis (Spreng.) Briq. (L. sellowiana Link & Otto) was investigated for its chemical composition and mosquito repellent activity. The essential oil obtained by hydrodistillation of aerial plant parts was analyzed by GC-FID and GC-MS. The major constituents were β-elemene (22.0%), β-caryophyllene (20.1%), and germacrene D (9.4%). Sesquiterpene hydrocarbons were present in considerable quantities (78.9%) in the L. montevidensis EO, followed by oxygenated sesquiterpenes (8.9%), monoterpene hydrocarbons (7.7%), oxygenated monoterpenes (1.9%), diterpenes (1.2%) and other compounds (0.2%). The oil of L. montevidensis was repellent with a minimum effective dosage (MED) of 0.021 ± 0.013 mg/cm2 as compared with that of the positive control N,N-diethyl-3-methylbenzamide (DEET) with a MED of 0.006 ± 0.001 mg/cm2) against Aedes aegypti L. The major compound β-elemene was tested individually for its repellency and had a MED value of 0.23 ± 0.14 mg/cm2 (DEET was 0.008 ± 0.001 mg/cm2). This is the first report on the repellent activity of L. montevidensis EO and β-elemene using human-based in vivo assays against Ae. aegypti.

scholarly journals Integrated Control of Aedes Aegypti With Parasitic Nematodes, Biological Control Agents, Chemical Insecticides and Plant Essential Oil

2021 ◽  
Author(s):  
Jinfeng Xiong ◽  
Hui Zhang ◽  
Caixia Li ◽  
Rui Ma ◽  
Hui Ai ◽  
...  
Keyword(s):  
Biological Control ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
High Efficiency ◽  
Pcr Analysis ◽  
Control Group ◽  
Mosquito Vector ◽  
Biological Control Agents ◽  
Competitive Binding Assay ◽  
Density Control

Abstract Aedes aegypti can transmit dengue fever, yellow fever, Chikungunya fever, Zika virus disease and vector density control is the most effective way to prevent these infectious diseases. However, the extensive use of chemical pesticides has caused a series of problems, such as environmental pollution, killing non-target organisms and so on. In this study, a parasitic nematode, Romanomermis wuchangensis was used in the larviciding evaluation of Ae. aegypti, while the activity of four chemical insecticides and biological control agents were tested. Besides, Mentha haplocalyx essential oil was isolated and its olfactory physiological function with OBP1 protein of Ae. aegypti antenna was measured by the prokaryotic expression and fluorescence competitive binding assay. Compared with the control group, R. wuchangensis indicated high efficiency and environmental friendliness in the control of Ae. aegypti. After the second instar larvae were parasitized, the mortality of two treatment groups exceeded 75%. Compared to control group, the quantitative real-time PCR analysis results demonstrated that SOD, POD and CAT genes had obvious high expression levels in the nematodes parasitic groups. The antioxidant enzyme test results also exhibited obvious difference of SOD, CAT and POD during the nematode parasitic period. Besides, Bacillus thuringiensis (Bti) and chemical insecticide experimental results also showed great insecticidal efficacy against mosquito larvae. Five chemical components including Menthol, Pinene, Limonene, Isopulegol and Pulegone were identified from M. haplocalyx and exhibited great binding ability with AaegOBP1 protein. Present results illustrated that the integrated application of these various mosquito vector control methods in the future has broad prospects.

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