scholarly journals The effect of hormonal treatment with kisspeptin, GnRH and hCG on semen characteristics in buck Cyprus goats during non-breeding season as compared with breeding season

2019 ◽  
Vol 12 (1) ◽  
pp. 74-88
Author(s):  
Maad AL-Ameri ◽  
Talal Abdulkareem ◽  
Ahmed Taha
Keyword(s):  
Body Weight ◽  
Breeding Season ◽  
Normal Saline ◽  
Hormonal Treatment ◽  
Post Treatment ◽  
Abu Ghraib ◽  
Plasma Testosterone ◽  
Control Group ◽  
Testosterone Concentration ◽  
Semen Characteristics

The current study aimed to determine the effect of hormonal treatment with kisspeptin, GnRH and hCG on plasma testosterone concentration and semen characteristics in buck Cyprus goats during non-breeding season as compared with breeding season. This study was executed at the Ruminant Researches Station pertaining to the Directorate of Agricultural Researches, Ministry of Agriculture, Abu-Ghraib, Baghdad (latitude 33˚20' N) for the period from November 15th, 2012 to December 〖31〗^st 2013. A total of 20 buck Cyprus goats 2 years old and averages 55 kg body weight. During non-breeding season bucks were randomly divided into five equal groups (4 bucks / group). The first group (A1) was regarded as a control group, i.m injected with normal saline, whereas, the second (A2) and third (A3) groups were i.v injected with 4 and 8 µg / kg body weight of Kisspeptin-10 respectively. The fourth (A4) and fifth (A5) groups were i.m injected with hCG (250 IU / buck) and GnRH (20 µg / buck) respectively. Plasma testosterone concentrations were significantly (P≤ 0.01 and P≤ 0.05) increased at 20, 30, 40 and 50 min post-treatment in A5, A2 and A3 groups as compared with control A1. Mass motility and individual motility were significantly (P≤ 0.01) in A5, A4, A2 and A3 as compared with control during non-breeding season. In conclusion, our results show that Kisspeptin-10, GnRH and hCG can stimulate the quiescent hypothalamic–pituitary–gonadal axis of bucks during non-breeding season by increasing plasma testosterone concentration post treatment that leads improving some semen characteristics during non-breeding season.

scholarly journals Peripheral Administration of RF9 Does Not Affect Hypothalamic-PituitaryGonadal Axis in Normal Fed Adult Male Macaque

2020 ◽  
pp. 1-5
Author(s):  
Aalia Batool ◽  
Madiha Wazir ◽  
Rahim Ullah ◽  
Aalia Batool ◽  
Rabia Naz ◽  
...  
Keyword(s):  
Adult Male ◽  
Normal Saline ◽  
Time Windows ◽  
Treated Group ◽  
Plasma Testosterone ◽  
Control Group ◽  
Hpg Axis ◽  
Testosterone Levels ◽  
Physiological Conditions ◽  
Short Time

Stress represses hypothalamic-pituitary-gonadal axis (HPG-axis) but RF9, a synthetic peptide, rescues such repression. To assess the role of RF9 in regulating HPG-axis under normal physiological conditions in higher primates, RF9 was administered to intact adult male rhesus monkeys and response of the HPG-axis was examined by measuring plasma testosterone as an end parameter of the axis. Control group (n=4) received normal saline whereas the treated group (n=4) received RF9. On the first day of experiment, four bolus injections of normal saline (1ml/animal) were administered intravenously at 2-hr interval to the control monkeys. Similarly, on the second day of experiment, treated group received four iv bolus injections of RF9 (0.1mg/kg BW) at 2-hr interval. Serial blood samples were collected at 20 min interval during a 6-hr period which started just after first saline/RF9 injection. Plasma testosterone levels were measured by using a specific EIA. Overall means of plasma testosterone levels and plasma testosterone area under curve (AUC) and overall mean testosterone and mean testosterone AUC in short time windows following each injection of RF9 and saline were comparable between the groups. Our results demonstrate that RF9 has no role in regulating HPG-axis under normal physiological conditions in adult male monkeys.

scholarly journals A COMPARATIVE EVALUATION OF ANTI-DIABETIC POTENTIALITY FOUND IN DIFFERENT MARKETED POLYHERBAL FORMULATION USING GLUCOCORTICOID-INDUCED HYPERGLYCAEMIA IN RABBIT

2017 ◽  
Vol 9 (4) ◽  
pp. 83
Author(s):  
Pranjal Boruah ◽  
Jashabir Chakraborty ◽  
Suvakanta Dash
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Blood Glucose ◽  
Normal Saline ◽  
Side Effect ◽  
Body Weight Loss ◽  
Control Group ◽  
Therapeutic Equivalence ◽  
Polyherbal Formulation ◽  
Single Dosage

Objective: The aim of this study was performed to evaluate Antidiabetic potentiality found in different marketed polyherbal formulation using glucocorticoid-induced hyperglycaemia in the rabbit.Methods: The potentiality of different polyherbal formulation was investigated using dexamethasone (DEX) induced hyperglycaemia in Rabbit. Eight male rabbits were divided into four groups of two each. The first group is regarded as control group received 3 ml of normal saline daily by using the gastric tube for 15 d and remaining three group received (0.35 mg/Kg B.W. single dosage) of dexamethasone tablets which were powdered, dissolved in 3 ml of normal saline daily for 15 d. After 15 d the blood glucose estimated by using a glucometer and it is found that DXE treatment leads to significant increase in levels of glucose and a significant decrease in body weight. After that second group received metformin tablet. The third and fourth group received polyherbal formulation A and formulation B, which are powdered and dissolved in 3 ml of normal saline daily for 15 d at the dose of 0.5 gm/kg body weight orally. After completion of regular administration for 15 d, the blood glucose was again estimated and compare the results of each the group.Conclusion: The Anti-diabetic polyherbal marketed formulations were having less side effect as compared to standard metformin tablet (e. g. body weight loss). And both the polyherbal formulations were found a therapeutic equivalence to each other, also having the approximately similar potentiality to standard metformin tablet.Results: The result was found that the polyherbal marketed formulations were having less side effect as compared to standard metformin tablet (e. g. body weight loss). And both the polyherbal formulations were found significantly decreased in blood glucose level at equal potentiality, which can be consider as therapeutic equivalence to each other, and both the formulation also having the approximately similar potentiality to standard metformin tablet. 

scholarly journals A study of the teratogenic effect of Ciprofloxacin

1970 ◽  
pp. 9-11
Author(s):  
Selim Mohammed Jahangir ◽  
AFM Saiful Islam
Keyword(s):  
Body Weight ◽  
Strong Evidence ◽  
Normal Saline ◽  
Teratogenic Effect ◽  
Control Group ◽  
Modern Drug ◽  
Pregnant Mice ◽  
Experimental Group ◽  
Proven Benefit ◽  
In Pregnancy

Drugs or medicinal agent should only be used in pregnancy if there is proven benefit to the mother and no potential teratogenic risks. Present study was performed to evaluate the teratogenic effect of modern drug, Ciprofloxacin a Fluoroquinolone derivative, which has been introduced in Bangladesh. This was felt necessary because this has been found to be used indiscriminately at all levels of the prescribers and there is no strong evidence to its safety in pregnancy The work described here is to evaluate the teratogenic effect of Ciprofloxacin using swiss albino mice. The pregnant mice were divided into two different groups which received Normal Saline and Ciprofloxacin [25 mg/kg body weight.] given from 6th to 15th day of gestation. Neither the control group nor the experimental group revealed any sign of external deformity and cleft palate. DOI: 10.3329/bjpp.v22i1.3562 Bangladesh J Physiol Pharmacol 2006; 22(1/2) : 9-11

The influence of injectable sodium selenite on semen characteristics and testosterone concentration in Aardi goats

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
A. Hajalshaikh ◽  
M. J. Al-Hassan ◽  
H. E. Mohamed
Keyword(s):  
Body Weight ◽  
Sodium Selenite ◽  
Semen Quality ◽  
Sperm Count ◽  
Plasma Concentrations ◽  
Control Group ◽  
Progressive Motility ◽  
Group I ◽  
Semen Characteristics ◽  
And Control

The objective of this study was to test the effects of sodium selenite (SS) injection on semen characteristics and testosterone plasma concentrations in male Aardi goats. Fifteen animals were assigned into three groups; control (group I, with no supplemental SS); group II; injected intramuscular with 0.1 mg/kg SS body weight; group III injected 0.05 mg/kg SS body weight. Blood samples were collected once weekly (week 0 and four weeks after treatment) in the morning (at 8 am). Semen was collected by electro-ejaculator on a weekly basis post SS supplementation. Sperm count, motility %, progressive motility characteristic; average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR) and linearity (LIN) were analyzed. Significant differences were found between the two treated-groups and control after week four for motility and progressive motility. After five weeks, significant differences were found between groups, for motility and progressive motility. The live % showed significant differences after five weeks between treated and control groups. After five weeks, significant differences were found between the three groups, for motility and progressive motility. The live % showed significant differences after 5 weeks between different treatments. However, VAP, VSL, VCL, STR, and LIN showed non-significant differences, suggesting that bucks might already have a good motility characteristic. No significant effects of SS supplementation on testosterone plasma levels, and the reported values were 1.28; 1.35, and 1.36 ng/ml for control; group 1, and group 2; respectively. In conclusion, SS improved reproduction in goats <italic>via</italic> the enhancement of semen quality in Aardi goats.

ENHANCED TESTOSTERONE IN FEMALE PATIENTS WITH ANOREXIA NERVOSA: ITS NORMALIZATION AFTER WEIGHT GAIN

1979 ◽  
Vol 90 (2) ◽  
pp. 328-335 ◽  
Author(s):  
B. Baranowska ◽  
S. Zgliczyński
Keyword(s):  
Body Weight ◽  
Anorexia Nervosa ◽  
Weight Gain ◽  
Serum Testosterone ◽  
Gonadal Hormones ◽  
High Serum ◽  
Control Group ◽  
Testosterone Concentration ◽  
Female Patients ◽  
Serum Lh

ABSTRACT In order to elucidate the mechanism of disturbances of gonadal hormones secretion in anorexia nervosa 14 female patients were investigated. A control group also consisted of 14 women of the same age. The serum LH, progesterone, oestrogens: oestrone + oestradiol (Oe1 + Oe2), oestriol (Oe3) and testosterone were determined by radioimmunological methods. In patients with anorexia nervosa the serum testosterone and Oe3 concentrations were dramatically elevated, whereas LH, progesterone and Oe1 + Oe2 were decreased as compared with the control group. Considerable weight gain induced by cyproheptadine treatment caused a normalization of the serum testosterone and Oe3 concentrations in all the patients. A negative correlation between the testosterone level and the deficit in body weight was observed. The mechanism causing the dramatically high serum testosterone concentration in the female patients with anorexia nervosa is discussed.

scholarly journals Influence of Different Feeding Ratio of Pomegranate Peels Powder on Testicular Dimensions, Body Weight and Testosterone Concentration in Karadi Male Lambs

2019 ◽  
Vol 12 (2) ◽  
Keyword(s):  
Body Weight ◽  
Blood Serum ◽  
Average Daily Gain ◽  
Serum Testosterone ◽  
Control Group ◽  
Final Body Weight ◽  
Testosterone Concentration ◽  
Scrotal Circumference ◽  
Total Body ◽  
Daily Gain

The current study was presented to conclude the influence of different ratio of pomegranate peel powder (PP) in feed of Karadi male lambs, they divided to 4 groups (0% as a control, 1% treatment 1, 2% treatment 2 and 4% as treatment 3), on scrotal circumference (SC), scrotum height (SH), body weight (BW) and blood serum testosterone concentration (T). In this study, sixteen (16) of Karadi male lambs used with 24.30 ± 0.40 kg weight and with 4-5 months old, all lambs have been randomly divided equally into four (4) groups and individually penned in a box for during 63 days. Results in our study showed that SC was significantly different and recorded higher 17.875±0.54 cm in group that fed on 1% PP, also SH recorded higher and significant different in group fed on 1% PP compared with other groups 10.000±0.32 cm. Average blood serum testosterone reduces in all groups compared to control group that fed on 0% of PP (11.418±0.15 n.mol/L). Final body weight in our study had significantly different (P < 0.05) between groups, 1% PP recorded higher weight (34.9 ± 0.58) kg. Each average daily gain, total body gain and initial body weight had not significant different in all groups. Correlation coefficient between SC, SH, blood serum testosterone concentration and final BW computed and ranged between (-0.397 to + 0.914).

scholarly journals EFFICACY OF IVERMECTIN AGAINST GASTROINTESTINAL NEMATODIASIS AND ECTOPARASITES IN CROSSBRED CATTLE IN BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 191-197
Author(s):  
M. Ahammed ◽  
M. A. Ali ◽  
M. A. Ehsan ◽  
M. Mostafa
Keyword(s):  
Body Weight ◽  
Treatment Period ◽  
Hematological Parameters ◽  
Research Work ◽  
Live Weight ◽  
Treated Group ◽  
Post Treatment ◽  
Control Group ◽  
Crossbred Cattle ◽  
Group A

The experiment was carried out to determine the efficacy of Ivermectin (Ivomec® S/C Formulation) against gastrointestinal nematodiasis and ectoparasites including stephanofilaria on crossbred cattle, to determine the effect of drug on certain hematological parameters like hemoglobin (Hb) content, packed cell volume (PCV) and erythrocyte sedimentation rate (ESR)in crossbred cattle and to determine the effect of the drug on live weight. A total of 100 cattle were selected randomly and examined for presence of both endo and ectoparasites including stephanofilaria. Gastrointestinal nematodiasis were detected by examination of fecal samples and ectoparasites as well as stephanofilaria were detected by physical examination. Out of 100 cattle, 20 were found to suffer from both endo and ectoparasites. These 20 cattle were selected finally for the research work. Then these cattle were divided into two groups, group A (treated group, n=15) and group B (control group, n=5). Ivermectin was injected subcutaneously to the cattle of group A @ 200µm/kg body weight (1ml/50 kg body weight). The therapeutic efficacy of the drug against gastrointestinal nematodiasis was determined by investigation of fecal egg count reduction and the efficacy was 100% against the common nematodes in crossbred cattle on day 7, 14, 21 and 28 of post treatment period. Ticks within a markable area (25 square inches) were counted on day 0 and lice infestation was marked as infected on day 0. Ivermectin showed 100% effectiveness at the 7, 14, 21 and 28 day of post treatment period against both tick and lice infestation. The efficacy of the drug against stephanofilariasis (humpsore) was determined by reduction of diameter of the sore on the day 14, 42 and 56 of post treatment period. All the sores were completely healed up by the 56 days. During the study of hematological parameters it was seen that Hb and PCV were increased whereas ESR values were decreased on post treatment days. In this study the mean live weight of the cattle of treated group was increased after treatment with ivermectin and increased body weight was 5.13% on the 28th day of post treatment period. From the above findings it may be concluded that ivermectin was 100% effective against gastrointestinal nematodes common in crossbred cattle. Effectiveness of the drug against external parasites was 100%. In this study hematological parameters were changed significantly after treatment with ivermectin, body weight of animal treated with ivermectin was increased and no adverse effect of the drug was found in this study.

An integrated approach to the management of patients with endometrial hyperplasia and metabolic syndrome

GYNECOLOGY ◽  
2021 ◽  
Vol 23 (1) ◽  
pp. 55-61
Author(s):  
Irina A. Lapina ◽  
Yulia E. Dobrokhotova ◽  
Lyudmila A. Ozolinya ◽  
Tatiana G. Chirvon ◽  
Vladislav V. Taranov
Keyword(s):  
Metabolic Syndrome ◽  
Body Weight ◽  
Endometrial Hyperplasia ◽  
Clinical Manifestations ◽  
Hormonal Treatment ◽  
Integrated Approach ◽  
Overweight And Obesity ◽  
Metabolic Parameters ◽  
Control Group ◽  
Control Value

Despite the achieved success in the treatment of endometrial hyperplasia processes, some issues related to the treatment of these diseases have not been completely resolved, especially in the presence of metabolic syndrome. Aim. To optimize the management of patients with endometrial hyperplasia and metabolic syndrome. Materials and methods. The study included 50 women with a verified diagnosis of recurrent endometrial hyperplasia. As a control value, a BMI of 25.0 kg/m2 was taken, two subgroups were identified with normal body weight and with overweight and obesity of the 1st degree. Treatment with GnRH was carried out in the following regimen: intramuscular injection of the drug at a dose of 3.75 mg once every 4 weeks with an assessment of the results after 3 and 6 months. In the course of the study, we assessed metabolic parameters, parameters of the hemostasis and fibrinolysis system before and during therapy. The baseline studied parameters were compared with the control group of healthy women without endometrial hyperplastic processes and overweight as well as patients of the control group with metabolic syndrome. Results. Hormonal treatment of recurrent endometrial hyperplasia without atypia using GnRH a positive effect on the dynamics of clinical manifestations, which is confirmed by the data of ultrasound and histological research methods and does not cause a sharp procoagulant shift, regardless of the patients body weight. Conclusion. Our results allow GnRH to be the drugs of choice in the treatment of endometrial hyperplasia in overweight patients, since they have a fairly safe and effective effect. It is necessary to remember about an integrated approach to patient management and to carry out the prevention of endothelial dysfunction in this group of patients due to the high risk of complications to potentiate a beneficial effect on hemostasiological and metabolic parameters.

Testicular function and semen characteristics of Awassi rams treated with melatonin out of the breeding season

2009 ◽  
Vol 57 (4) ◽  
pp. 531-540 ◽  
Author(s):  
Vera Faigl ◽  
Mónika Keresztes ◽  
Margit Kulcsár ◽  
Sándor Nagy ◽  
Zsuzsanna Keresztes ◽  
...  
Keyword(s):  
Breeding Season ◽  
Slow Release ◽  
Endocrine Function ◽  
Semen Parameters ◽  
Control Group ◽  
Melatonin Treatment ◽  
Semen Characteristics ◽  
Igf I ◽  
Semen Donor

The objective of this study was to evaluate the effect of long-term melatonin treatment applied during the non-breeding season on semen characteristics, endocrine function of testicles and baseline level of insulin-like growth factor-I (IGF-I) in Awassi rams kept in the temperate continental zone of Europe and used as semen donors in an artificial insemination (AI) programme. On 23 February (day 0), slow-release melatonin implants were inserted subcutaneously into rams (n = 8). Control animals (n = 8) received no treatment. In both groups, basic semen parameters (concentration, total motility, fast and slow forward motility, morphology), GnRH-induced testosterone response and basal IGF-I concentration were evaluated on days 0, 47 and 71. No differences were found in concentration of spermatozoa, total motility, and numbers of spermatozoa with fast and slow progressive motility and normal/abnormal morphology between the melatonin-treated and the control group. However, in melatonin-treated animals, basal and GnRH-induced testosterone levels were slightly elevated on day 47 and became significantly higher on day 71 (P < 0.05) as compared to controls. There was no difference in plasma IGF-I levels between the groups. In conclusion, slow-release melatonin applied during the non-breeding season improves testicular testosterone production but does not influence the semen characteristics and the IGF-I level of semen donor Awassi rams used in an AI programme and kept in the temperate continental zone of Europe.

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