scholarly journals Role of alcoholic extract of Roket (Eruca sativa) leaves on male reproduction of experimentally induced-oxidative stressed rats

2015 ◽  
Vol 39 (2) ◽  
pp. 47-54
Author(s):  
Baraa Najim AL-Okaily
Keyword(s):  
Hydrogen Peroxide ◽  
Epithelial Cells ◽  
Tap Water ◽  
Ethanolic Extract ◽  
Protective Role ◽  
Male Reproduction ◽  
Seminiferous Tubules ◽  
Eruca Sativa ◽  
Histological Sections

     This study conducted to find out the protective role of ethanolic extract of Eruca sativa leaves against the deleterious effect of hydrogen peroxide on some aspect of male reproduction in adult rats. Forty adult male rats were randomly assigned into four equal groups as follows: control group received tap water (untreated); group T1 were received tap water containing 0.5% H2O2; group T2 were received tap water containing 0.5% H2O2 plus administration of 300 mg/kg. B.W. ethanolic extract of Eruca sativa leaves and group T3 administration ethanolic extract of Eruca sativa leaves only at the same dose of group T2. All treatments continued for 60 days. At the end of the experiment, samples of tests and epididymis tissues were taken to prepare histological sections for                                                                                                                                                                                                       measurement the diameter of seminiferous tubules, thickness of epithelial cells of seminiferous tubules and histological examination of testes and epididymis. The results in Group T1 showed a significant decrease in the diameter and thickness of epithelial cells of seminiferous tubules, but these parameters clarified a significant increase in T2 and T3 groups as compared with T1 group. Histological sections of testis and epididymis in group T1 revealed incomplete spermatogenesis, cell debris, vacculation of Sertoli cells and view sperms in the lumen of seminiferous tubules and epididymis. Besides, normal obvious histological architecture of seminiferous tubules and epididymis with complete spermatogenesis were shown in sections of testis and epididymis of T2 and T3 groups' as compared to T1. In conclusion, hydrogen peroxide may impair spermatogenesis, furthermore, the results confirm the protective role of E. sativa leaves extract against oxidative stress induced by H2O2 in rats.

scholarly journals Role of Alpha Lipoic Acid in Ameliorating the Histological Alterations of Pituitary-Testicular Axis –induced by Hydrogen Peroxide in Rats

2020 ◽  
Vol 13 (2) ◽  
pp. 125-136
Author(s):  
Mohammad Alwan ◽  
Baraa Al-Okialy
Keyword(s):  
Hydrogen Peroxide ◽  
Lipoic Acid ◽  
Tap Water ◽  
Degenerative Changes ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Alpha Lipoic Acid ◽  
Water Group ◽  
Testicular Weight

The present study was aimed to investigate the role of alpha-lipoic acid ( ALA) as an antioxidant against in ameliorating histological disorders of pituitary- testicular axis- induced by hydrogen peroxide (H2O2) in adult Wistar rats. Forty adult male rats were randomly divided into four equal groups (10 rats /group) and were handled daily as follows for 56 days: Control group ( C) were intubated distilled water and received ordinary tap water; group T1 were intubated 60mg/kg B.W of ALA and received ordinary tap water; group T2 were administered H2O2 in tap water at a concentration of 0.05%, while group T3 were intubated 60mg/kg B.W of ALA and received ordinary tap water containing 0.05% H2O2. At the end of the experiment, body weights were recorded, then pituitary and testes were excised for histopathological study and testicular weight was recorded too. Rats administered H2O2 showed a significant decrease in testes weight to body weight ratio accompanied with major histopathological changes of the testes in comparison with other groups including; a significant decrease in the diameter of seminiferous tubules, high of germinal epithelial cell and degenerative changes with incomplete spermatogenesis. Besides, a significant decrease in the number of Leydig's cells in comparison with other experimental groups. Furthermore, pituitary gland of group T2 manifested a severe histological alteration in architecture characterized by atrophy with marked necrotic and degenerative changes. Whereas, rats administered ALA (group T3) shows an improvement of histological changes of pituitary and testicular tissues induced by hydrogen peroxide. In conclusion, the results indicated that alpha-lipoic acid mitigated pituitary-testicular dysfunctions induced by H2O2 through its antioxidant effects via scavenging free radicals.

scholarly journals Histological changes in pituitary-testes axis in rats exposed to cadmium chloride: Protective role of Eruca sativa seeds

2017 ◽  
Vol 41 (1) ◽  
pp. 76-85
Author(s):  
Baraa Najim Al-Okaily
Keyword(s):  
Pituitary Gland ◽  
Cadmium Chloride ◽  
Tap Water ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Interstitial Tissue ◽  
Eruca Sativa

     The present study was carried out to investigate the protective effect of Eruca sativa seeds against changes of pituitary and testes in cadmium treated rats. Forty adult male albino rats were used and divided into 4 equal groups and treated for 8 weeks as follow: The first group was given distilled water and used as a control group. The second group (G1) was administered tap water containing 30 ppm/ L of cadmium chloride. Animals in third group (G2) received tap water containing Cadmium chloride as in G1 plus 250 mg/Kg B.W ethanolic extract of Eruca sativa seeds, while rats in fourth group (G3) administered 250 mg/Kg B.W of ethanolic extract of Eruca sativa seeds only. After 8 weeks of experiment the animals were anesthetized, the pituitary and testes were excised for histological studies. Results showed that cadmium chloride intake caused severe alterations in the pituitary gland manifested by a marked  apoptotic  cells  of  pars distalis, accompanied with marked necrosis leading to left large multiple spaces in their parenchyma. Whereas testes revealed shrinkage, irregular arrangement of seminiferous tubules and increase intertubular spaces. A considerable changes was also found in the seminiferous tubules with loss most of their epithelial layer and filled with cellular debris, incomplete spermatogenesis, congested blood vessels and few Leydig's cells. Administration of Eruca sativa extract reduce the deleterious effects of cadmium chloride on pituitary gland as well as more or less complete spermatogenesis process with thickness of interstitial tissue in most of seminiferous tubules. It could be suggested that the antioxidant properties of Eruca sativa extract protects the deleterious histological damage induced by cadmium chloride in adult rats.

PROTECTIVE ROLE OF FICUS RACEMOSA IN DIABETES INDUCED NEUROPATHY: STRUCTURAL AND FUNCTIONAL EVIDENCES

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (11) ◽  
pp. 58-60
Author(s):  
N Solanki ◽  
◽  
S. K Bhavsar
Keyword(s):  
Sciatic Nerve ◽  
Diabetic Neuropathy ◽  
Ethanolic Extract ◽  
Protective Role ◽  
Diabetic Rats ◽  
Dependent Manner ◽  
Traditional System ◽  
Ficus Racemosa ◽  
Dose Dependent

Ficus racemosa is used in traditional system of medicine for various health problems and diseases, and is commonly known as Gular fig. The main objective was to study its effects against streptozotocin induced diabetic neuropathy by structural and functional marker. Investigation of diabetic neuropathy was carried out through functional and structural assessment in streptozotocin induced in diabetic rats. Diabetic rats were treated for 28 days in dose dependent manner of Ficus racemosa aqueous extract (250 mg/kg and 500 mg/kg) and ethanolic extract (200 mg/kg and 400 mg/kg). Study showed marked protection observed by Ficus racemosa in hippocampus region of brain and sciatic nerve tissues. Ficus racemosa treatment showed improvement in functional and structural markers, which strongly suggest its protective role in diabetic neuropathy.

Cytotoxic effects of cadmium in mammary epithelial cells: Protective role of the macrocycle [15]pyN5

2012 ◽  
Vol 50 (6) ◽  
pp. 2180-2187 ◽  
Author(s):  
Sandrina Gonçalves ◽  
Ana Sofia Fernandes ◽  
Nuno G. Oliveira ◽  
Joana Marques ◽  
Judite Costa ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Protective Role ◽  
Mammary Epithelial ◽  
Cytotoxic Effects

Protective role of fructose in the metabolism of astroglial C6 cells exposed to hydrogen peroxide

2009 ◽  
Vol 344 (13) ◽  
pp. 1676-1681 ◽  
Author(s):  
Ivan Spasojević ◽  
Aleksandar Bajić ◽  
Katarina Jovanović ◽  
Mihajlo Spasić ◽  
Pavle Andjus
Keyword(s):  
Hydrogen Peroxide ◽  
Protective Role ◽  
C6 Cells

scholarly journals Protective Role of Agrimonia eupatoria L. in Heavy Metal Induced Nephrotoxicity

2018 ◽  
Vol 62 (2) ◽  
pp. 13-18
Author(s):  
D. Marcinčáková ◽  
M. Falis ◽  
P. Schusterová ◽  
P. Váczi ◽  
S. Marcinčák ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Potassium Dichromate ◽  
Ethanolic Extract ◽  
Protective Role ◽  
Rabbit Kidney ◽  
Cell Adherence ◽  
Metal Compounds ◽  
Sulfate Heptahydrate ◽  
Chloride Dihydrate

Abstract The aim of this study was to evaluate the potential protective role of Agrimonia eupatoria L. in heavy metal induced nephrotoxicity. Rabbit kidney epithelial cells (RK13) were used as the model cell line. They were exposed to three different heavy metal compounds: cadmium chloride dihydrate CdCl2.2H2O (15 and 20 mg.l−1), potassium dichromate K2Cr2O7 (1, 10 mg.l−1), and zinc sulfate heptahydrate ZnSO4.7H2O (50, 150 mg.l−1) simultaneously with agrimony (ethanolic extract, 100 mg.l−1). The cell response was recorded using the xCELLigence system or real-time cell analysis (RTCA) as a cell index (CI) and expressed as cell adherence (%) compared to control cells without treatment. The potential nephroprotective effects were recorded in cells treated with chromium (1 a 10 mg.l−1) and agrimony, where the cell adherence increased from 95.11 ± 11.25 % and 7.24 ± 0.33 % to 103.26 ± 1.23 % and 68.54 ± 4.89 % (P < 0.05) respectfully and also with a combination of agrimony and zinc (150 mg.l−1), where the adherence increased from 57.45 ± 1.98 % to 95.4 ± 6.95 %. During the cell exposure to cadmium in combination with agrimony, the protective effect was not recorded; the adherence of cells was even decreased (P < 0.05).

scholarly journals Protective role of Indoleamine 2,3 dioxygenase in Respiratory Syncytial Virus associated immune response in airway epithelial cells

Virology ◽  
2017 ◽  
Vol 512 ◽  
pp. 144-150 ◽  
Author(s):  
Devi Rajan ◽  
Raghavan Chinnadurai ◽  
Evan L. O'Keefe ◽  
Seyhan Boyoglu-Barnum ◽  
Sean O. Todd ◽  
...  
Keyword(s):  
Immune Response ◽  
Respiratory Syncytial Virus ◽  
Epithelial Cells ◽  
Protective Role ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Indoleamine 2,3 Dioxygenase ◽  
Syncytial Virus
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