A spirostanol glycoside from wild yam (Diosccorea villosa) extract and its cytostatic activity on three cancer cells

We studied the estrogenic activity and cellular effect of wild yam extract in MCF-7 human breast cancer cells. The extract increased the activity of the progesterone receptor and pS2 genes at the mRNA levels in human breast cancer MCF-7 cells, although the effects were not as prominent as those of 17β-estradiol (E2). Western blot analysis showed that the level of estrogen receptor α protein was down-regulated after treatment with E2 or wild yam extract. Wild yam extract also inhibited proliferation of MCF-7 cells. These data indicate that wild yam extract acts as a weak phytoestrogen and protects against proliferation in human breast carcinoma MCF-7 cells.

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Secondary Metabolites from a Culture of the Fungus Neosartorya pseudofischeri and Their In Vitro Cytostatic Activity in Human Cancer Cells

Planta Medica ◽

10.1055/s-0032-1315301 ◽

2012 ◽

Vol 78 (16) ◽

pp. 1767-1776 ◽

Cited By ~ 22

Author(s):

Amnat Eamvijarn ◽

Anake Kijjoa ◽

Céline Bruyère ◽

Véronique Mathieu ◽

Leka Manoch ◽

...

Keyword(s):

Secondary Metabolites ◽

Cancer Cells ◽

Human Cancer ◽

Cytostatic Activity ◽

Human Cancer Cells

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Оценка эффективности противоопухолевого воздействия и индукции апоптоза в клетках карциномы почки человека биологически активными добавками, содержащими ресвератрол, индол-3-карбинол и кордицепин, флуоресцентными методами визуализации

Оптика и спектроскопия ◽

10.21883/os.2021.06.50983.3k-21 ◽

2021 ◽

Vol 129 (6) ◽

pp. 727

Author(s):

Н.В. Полуконова ◽

Д.С. Исаев ◽

А.М. Мыльников ◽

А.Б. Бучарская ◽

А.В. Полуконова ◽

...

Keyword(s):

Cancer Cells ◽

Kidney Cancer ◽

Human Tumor ◽

Human Kidney ◽

Antitumor Agent ◽

Cytostatic Activity ◽

Antitumor Effects ◽

Human Renal Cell Carcinoma ◽

Low Concentrations

We compared the effectiveness of antitumor effects and apoptosis induction on human renal cell carcinoma A498 extracts of cruciferous plant material (indole-3-carbinol), Chinese mushroom (cordycepin), French red wine (resveratrol) at low concentrations after 24 and 48 h using fluorescent methods of imaging apoptosis and necrosis in human tumor cells in vitro . Propidium iodide and acridine orange were used as dyes in the "alive and dead" test, which allowed us to detect the number of dead cells and cells in which apoptosis had started. It was found that indole-3-carbinol at low concentrations (0.0288 and 0.1152 mg/ml) has a pronounced cytotoxic and cytostatic activity against human kidney cancer cells, significantly exceeding the action of resveratrol at the same concentrations. At the same time, cordycepin has no cytotoxic and cytostatic activity at these concentrations. Indole-3-carbinol also has the most pronounced apoptotic activity at concentrations of 0.0144-0.1152 mg/ml, after 48 h the number of kidney cancer cells in apoptosis increases by 6.8-10 times compared to control. It is concluded that indole-3-carbinol is a promising antitumor agent for use in the complex therapy of patients with kidney cancer.

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Evaluation of wild yam (Dioscorea villosa) root extract as a potential epigenetic agent in breast cancer cells

In Vitro Cellular & Developmental Biology - Animal ◽

10.1007/s11626-014-9807-5 ◽

2014 ◽

Vol 51 (1) ◽

pp. 59-71 ◽

Cited By ~ 5

Author(s):

Pranapda Aumsuwan ◽

Shabana I. Khan ◽

Ikhlas A. Khan ◽

Bharathi Avula ◽

Larry A. Walker ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Root Extract ◽

Wild Yam

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Carboranyl Derivatives of Rofecoxib with Cytostatic Activity against Human Melanoma and Colon Cancer Cells

Scientific Reports ◽

10.1038/s41598-020-59059-3 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Antonio Buzharevski ◽

Svetlana Paskaš ◽

Menyhárt-Botond Sárosi ◽

Markus Laube ◽

Peter Lönnecke ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Human Melanoma ◽

Cytostatic Activity ◽

Colon Cancer Cells ◽

Derivatives Of

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Cytoprotective Compounds Interfere with the Nutraceutical Potential of Bread Supplemented with Green Coffee Beans

Antioxidants ◽

10.3390/antiox8070228 ◽

2019 ◽

Vol 8 (7) ◽

pp. 228

Author(s):

Urszula Gawlik-Dziki ◽

Marcin Luty ◽

Dariusz Dziki ◽

Michał Świeca ◽

Katarzyna Piwowarczyk ◽

...

Keyword(s):

Cancer Cells ◽

Negative Impact ◽

Cytostatic Activity ◽

Wheat Bread ◽

Green Coffee ◽

Cytoprotective Activity ◽

Phenolic Contents ◽

Green Coffee Beans ◽

Coffee Beans ◽

Motile Activity

The proliferation and motile activity of prostate epithelial (Pnt2) and cancer cells (DU-145; PC-3) in the presence of bioavailable compounds from green coffee beans (GCB), wholemeal wheat bread (WMWB), and its GCB-fortified variant were analyzed. The considerable cytostatic and anti-invasive activity of GCB extracts was correlated with its phenolic contents. WMWB extract contained significantly lower levels of phenolics but still displayed relatively high cytostatic activity. However, the cytostatic properties of WMWB compounds were hardly augmented by 3% GCB flour supplementation. The cytoprotective activity of the WMWB compounds exerts a negative impact on the cytostatic activity of GCB compounds. These data confirm the relatively high chemopreventive potential of GCB. However, they also indicate that subtle interactions between bioavailable compounds in GCB and WMWB can negatively affect the nutraceutic potential of the fortified bread. Apparently, gastrointestinal processing differentially regulates the availability of individual compounds and affects the balance between the cytostatic and cytoprotective activity of the whole product. Our data show that comprehensive research is necessary before the fortification of a specific carrier with a specific supplement can be recommended.

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Isoliquiritigenin Nanosuspension Enhances Cytostatic Effects in A549 Lung Cancer Cells

Planta Medica ◽

10.1055/a-1134-3378 ◽

2020 ◽

Vol 86 (08) ◽

pp. 538-547 ◽

Cited By ~ 1

Author(s):

Fangxia Qiao ◽

Yue Zhao ◽

Yaping Mai ◽

Jueshuo Guo ◽

Luning Dong ◽

...

Keyword(s):

Electron Microscopy ◽

Cancer Cells ◽

A549 Cells ◽

Cytostatic Activity ◽

Licorice Root ◽

Normal Cells ◽

X Ray ◽

Pure Drug ◽

Pvp K30

AbstractIsoliquiritigenin, a flavonoid extracted from licorice root, has been shown to be active against most cancer cells; however, its antitumor activity is limited by its poor water solubility. The aim of this study was to develop a stable isoliquiritigenin nanosuspension for enhanced solubility and to evaluate its in vitro cytostatic activity in A549 cells. The nanosuspension of isoliquiritigenin was prepared through wet media milling with HPC SSL (hydroxypropyl cellulose-SSL) and PVP K30 (polyinylpyrrolidone-K30) as stabilizers, and the samples were then characterized according to particle size, zeta-potential, SEM (scanning electron microscopy), TEM (transmission electron microscopy), DSC (differential scanning calorimetry), XRPD (X-ray powder diffraction), FTIR (Fourier transform infrared spectroscopy), XPS (X-ray photoelectron spectroscopy), and in vitro release. The isoliquiritigenin nanosuspension prepared with HPC SSL and PVP K30 had particle sizes of 238.1 ± 4.9 nm and 354.1 ± 9.1 nm, respectively. Both nanosuspensions showed a surface charge of approximately − 20 mV and a lamelliform or ellipse shape. The dissolution of isoliquiritigenin from the 2 nanosuspensions was markedly higher than that of free isoliquiritigenin. In vitro studies on A549 cells indicated that the cytotoxicity and cellular uptake significantly improved after treatment with both nanosuspensions in comparison to the isoliquiritigenin solution. Furthermore, cell apoptosis analysis showed a 7.5 – 10-fold increase in the apoptosis rate induced by both nanosuspensions compared with pure drug. However, the cytotoxicity of pure drug and nanosuspension on normal cells (HELF) was lower, which indicated both isoliquiritigenin nanosuspensions have low toxicity to normal cells. Therefore, the isoliquiritigenin nanosuspension prepared with HPC SSL and PVP K30 as stabilizers may be a promising approach to improve the solubility and cytostatic activity of isoliquiritigenin.

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